iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000135913.10	ENST00000258399.7	ENSP00000258399.3
ENSG00000135913.10	ENST00000415516.5	ENSP00000400902.1
ENSG00000135913.10	ENST00000338465.5	ENSP00000345043.5

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
TargetScan	RepTar	SomamiR	miRcode
RAID2
Protein-protein Interaction
IID	PINA	HINT	Mentha
InWeb_IM
Protein 3D Structure
PDB	MMDB
Disease-associated Information
GWASdb
Drug and target
TTD
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	Phospho.ELM	UniProt	PHOSIDA
BioGRID	mUbiSiDa
DNA Methylation
MethyCancer	TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
DUB/USP	USP	14715245
UBD/Alpha-Helix/UIM	UIM	11406394

Classification

Family	E-value	Score	Start	End
DUB/USP	2.60e-88	298.1	341	950
UBD/Alpha-Helix/UIM	0.0013	20.2	807	824
UBD/Alpha-Helix/UIM	0.00036	12.7	828	846

Active Site

Position(s)	Description	Evidence
350	Nucleophile	{ECO:0000269\|PubMed:27296872}
906	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrelllekkkeklneenpkepkgellkslqklfeelw..kskkkavsprellkalkkeaeefsgyeqq 90
           +G++NlgNtCYmN++LQ+L+++++++++ll+   + ++++  k p ++l++ +++l+ +    +s++k+   +++++a++++ae+fsgy+q+
   Q: 341  QGFSNLGNTCYMNAILQSLFSLQSFANDLLK---QGIPWK--KIPLNALIRRFAHLLVKKDicNSETKKDLLKKVKNAISATAERFSGYMQN 427
           69*****************************...*****9..679999999999999776567***************************** PP
   S: 91   DaqEflaflldqleeelkkeknkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpvpgkeet 182
           Da+Efl+++ldql+e+++k  nk++++e  + +++++d+   + ++ +++++ ++++++++++i+c++c+++  ++e+f+dls+++p++++ 
   Q: 428  DAHEFLSQCLDQLKEDMEK-LNKTWKTEPVSGEENSPDI---SATRAYTCPVITNLEFEVQHSIICKACGEIIPKREQFNDLSIDLPRRKKP 515
           *****************55.9999999999999999999...7999*****************************************99988 PP
   S: 183  kke.slqdcLesftkeeelegenkyycekckkkqeaekklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfcts...... 267
            ++ s+qd+L+ f+++eele    y+cekc +k+ a  ++k+++lP+vLilhLkR+sf+ + + ++ki ++v +p+ L+ls++ct+      
   Q: 516  LPPrSIQDSLDLFFRAEELE----YSCEKCGGKC-ALVRHKFNRLPRVLILHLKRYSFNVALSLNNKIGQQVIIPRYLTLSSHCTEntkppf 602
           6545***************9....*********9.89999**********************************************99**** PP
   S: 268  ............................................................................................ 267
                                                                                                       
   Q: 603  tlgwsahmaisrplkasqmvnscitspstpskkftfksksslalcldsdsedelkrsvalsqrlcemlgneqqqedlekdsklcpiepdkse 694
           *************9999999999999999999999999999999999999999999999999999999999888888888888888888888 PP
   S: 268  ............................................................................................ 267
                                                                                                       
   Q: 695  lensgfdrmseeellaavleiskrdaspslshedddkptsspdtgfaeddiqempenpdtmetekpktiteldpasfteitkdcdenkenkt 786
           88888888888888888888888888888887777777777777777777777777777777777777777776666666666665555555 PP
   S: 268  peekqke..................................................................................... 274
           p     e                                                                                     
   Q: 787  P-----Egsqgevdwlqqydmerereeqelqqalaqslqeqeaweqkedddlkratelslqefnnsfvdalgsdedsgnedvfdmeyteaea 873
           0.....05555555555555555555556666666666666677777777777777777777888888888888888888888888888888 PP
   S: 275  ............kkkYeLiavvvHsG.slssGHYvayvknekgk.WllfdDskVtevkeeevleetgstaYvlfYkr 337
                       +++Y+Li+vv+H G ++ssGHY+++v++ k++ W++++D +V++++e+ v++++++++Y++fY++
   Q: 874  eelkrnaetgnlPHSYRLISVVSHIGsTSSSGHYISDVYDIKKQaWFTYNDLEVSKIQEAAVQSDRDRSGYIFFYMH 950
           888899999999****************************99999******************************98 PP

UBD/Alpha-Helix/UIM

   S: 2    EdeeLqlAlalSlqEeee 19
           E++eLq+Ala+SlqE+e 
   Q: 807  EEQELQQALAQSLQEQEA 824
           9**************985 PP

UBD/Alpha-Helix/UIM

   S: 1    dEdeeLqlAlalSlqEeee 19
           +Ed++L +A +lSlqE ++
   Q: 828  KEDDDLKRATELSLQEFNN 846
           69**************876 PP

Organism

Homo sapiens

Functional Description
(View)

Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) (PubMed:21596315). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1 (PubMed:27296872).

Protein Sequence
(Fasta)

MSPLKIHGPI RIRSMQTGIT KWKEGSFEIV EKENKVSLVV HYNTGGIPRI FQLSHNIKNV 60
VLRPSGAKQS RLMLTLQDNS FLSIDKVPSK DAEEMRLFLD AVHQNRLPAA MKPSQGSGSF 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GTGCGTGCGT GTTTGCGTGT GGTCACAGTT CCCGCCATCA GCCGCTCTCC CTGACTAGGG 60
GTAGCTAGCG GCCTCCCTCC TTTGGGTTCG GTATCCCTAC TTCCATTACC CTCGGCTCCT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0131--Cell cycle
KW-0132--Cell division
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0498--Mitosis
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-0645--Protease
KW-1185--Reference proteome
KW-0677--Repeat
KW-0788--Thiol protease
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR001394--Peptidase_C19_UCH
IPR003903--UIM_dom
IPR032069--USP37-like_PH
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS50330--UIM
PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF00443--UCH
PF16674--UCH_N
PF02809--UIM

SMART

SM00726--UIM

Gene Ontology

GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0004197--F:cysteine-type endopeptidase activity
GO:0019901--F:protein kinase binding
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0036459--F:thiol-dependent ubiquitinyl hydrolase activity
GO:0051301--P:cell division
GO:0000082--P:G1/S transition of mitotic cell cycle
GO:0007067--P:mitotic nuclear division
GO:0016579--P:protein deubiquitination
GO:0035871--P:protein K11-linked deubiquitination
GO:0071108--P:protein K48-linked deubiquitination
GO:0006275--P:regulation of DNA replication
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

hsa:57695

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001400	Ailuropoda melanoleuca	95.52	0.00e+00	1743.00
IUUC-Apl-004033	Anas platyrhynchos	77.47	0.00e+00	1435.00
IUUC-Aca-005370	Anolis carolinensis	69.73	0.00e+00	1312.00
IUUC-Ame-011884	Astyanax mexicanus	57.43	0.00e+00	985.00
IUUC-Bta-012725	Bos taurus	94.69	0.00e+00	1711.00
IUUC-Cja-019939	Callithrix jacchus	97.04	0.00e+00	1715.00
IUUC-Cfa-020835	Canis familiaris	95.72	0.00e+00	1798.00
IUUC-Cpo-022067	Cavia porcellus	93.43	0.00e+00	1559.00
IUUC-Cre-022867	Chlamydomonas reinhardtii	29.63	2.00e-24	107.00
IUUC-Csa-023085	Chlorocebus sabaeus	98.88	0.00e+00	1820.00
IUUC-Cho-024390	Choloepus hoffmanni	87.11	2.00e-165	576.00
IUUC-Cin-025496	Ciona intestinalis	37.25	1.00e-42	168.00
IUUC-Csv-025812	Ciona savignyi	36.22	7.00e-42	165.00
IUUC-Dre-028255	Danio rerio	57.26	0.00e+00	946.00
IUUC-Dno-028986	Dasypus novemcinctus	94.40	0.00e+00	1697.00
IUUC-Dor-031062	Dipodomys ordii	80.00	0.00e+00	887.00
IUUC-Ete-032359	Echinops telfairi	82.76	0.00e+00	817.00
IUUC-Eca-033964	Equus caballus	94.29	0.00e+00	1732.00
IUUC-Eeu-034492	Erinaceus europaeus	86.21	0.00e+00	824.00
IUUC-Fca-036359	Felis catus	94.90	0.00e+00	1734.00
IUUC-Fal-037640	Ficedula albicollis	77.58	0.00e+00	1405.00
IUUC-Gmo-039600	Gadus morhua	54.51	0.00e+00	933.00
IUUC-Gga-040126	Gallus gallus	77.37	0.00e+00	1368.00
IUUC-Gac-041557	Gasterosteus aculeatus	53.91	0.00e+00	914.00
IUUC-Ggo-045571	Gorilla gorilla	95.69	0.00e+00	1609.00
IUUC-Itr-048207	Ictidomys tridecemlineatus	94.79	0.00e+00	1700.00
IUUC-Lch-050357	Latimeria chalumnae	64.16	0.00e+00	1036.00
IUUC-Loc-052849	Lepisosteus oculatus	61.37	0.00e+00	1004.00
IUUC-Laf-054570	Loxodonta africana	92.36	0.00e+00	1643.00
IUUC-Mcc-054989	Macaca mulatta	98.77	0.00e+00	1684.00
IUUC-Meu-056273	Macropus eugenii	64.73	4.00e-171	595.00
IUUC-Mga-059814	Meleagris gallopavo	75.76	0.00e+00	1375.00
IUUC-Mmr-061726	Microcebus murinus	95.10	0.00e+00	1746.00
IUUC-Mdo-062974	Monodelphis domestica	85.22	0.00e+00	1572.00
IUUC-Mmu-064069	Mus musculus	90.41	0.00e+00	1619.00
IUUC-Mpu-066008	Mustela putorius furo	94.90	0.00e+00	1703.00
IUUC-Nle-069259	Nomascus leucogenys	99.29	0.00e+00	1785.00
IUUC-Opr-070882	Ochotona princeps	78.42	1.00e-140	494.00
IUUC-Ont-072441	Oreochromis niloticus	54.65	0.00e+00	927.00
IUUC-Oan-073151	Ornithorhynchus anatinus	76.26	7.00e-119	420.00
IUUC-Ocu-074879	Oryctolagus cuniculus	93.57	0.00e+00	1714.00
IUUC-Ola-087007	Oryzias latipes	53.35	0.00e+00	885.00
IUUC-Oga-088385	Otolemur garnettii	93.81	0.00e+00	1729.00
IUUC-Oar-090305	Ovis aries	92.76	0.00e+00	1736.00
IUUC-Ptr-091299	Pan troglodytes	99.80	0.00e+00	1808.00
IUUC-Pan-091996	Papio anubis	98.85	0.00e+00	1765.00
IUUC-Psi-093176	Pelodiscus sinensis	77.82	0.00e+00	1435.00
IUUC-Pma-094331	Petromyzon marinus	48.62	0.00e+00	768.00
IUUC-Ppa-095732	Physcomitrella patens	31.09	9.00e-25	108.00
IUUC-Pfo-096503	Poecilia formosa	56.29	0.00e+00	928.00
IUUC-Pab-098543	Pongo abelii	98.01	0.00e+00	960.00
IUUC-Pca-101099	Procavia capensis	87.20	0.00e+00	1298.00
IUUC-Pva-103163	Pteropus vampyrus	88.16	0.00e+00	1603.00
IUUC-Rno-105766	Rattus norvegicus	91.43	0.00e+00	1636.00
IUUC-Sha-106610	Sarcophilus harrisii	81.37	0.00e+00	1472.00
IUUC-Sar-113256	Sorex araneus	89.22	4.00e-110	392.00
IUUC-Ssc-116353	Sus scrofa	94.60	0.00e+00	1689.00
IUUC-Tgu-116971	Taeniopygia guttata	77.37	0.00e+00	1372.00
IUUC-Tru-117932	Takifugu rubripes	45.12	0.00e+00	664.00
IUUC-Tsy-119185	Tarsius syrichta	90.48	0.00e+00	1129.00
IUUC-Tni-119842	Tetraodon nigroviridis	60.13	1.00e-110	393.00
IUUC-Tbe-127281	Tupaia belangeri	83.68	0.00e+00	656.00
IUUC-Ttr-128177	Tursiops truncatus	94.81	0.00e+00	1340.00
IUUC-Vpa-130031	Vicugna pacos	94.90	0.00e+00	1770.00
IUUC-Xtr-132949	Xenopus tropicalis	65.22	0.00e+00	1162.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence