iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-046204

UUCD1 version

UUC-HoS-01163

Ensembl Protein ID

ENSP00000285679.6

UniProt Accession

Q9UHP3; C0LSZ0; Q6DHZ9; Q9H9W1; UBP25_HUMAN

Genbank Protein ID

AAF32263.1; AAF24998.1; ACN76567.1; EAX10041.1; AAH75792.1; BAB14107.1

Protein Name

Ubiquitin carboxyl-terminal hydrolase 25; Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25

Genbank Nucleotide ID

AF170562; AF134213; FJ763652; CH471079; BC075792; AK022574

Gene Name

USP25; USP21

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000155313.15	ENST00000285679.10	ENSP00000285679.6
ENSG00000155313.15	ENST00000351097.9	ENSP00000299574.6
ENSG00000155313.15	ENST00000285681.6	ENSP00000285681.2
ENSG00000155313.15	ENST00000453553.1	ENSP00000404086.1
ENSG00000155313.15	ENST00000449491.1	ENSP00000402354.1
ENSG00000155313.15	ENST00000400183.6	ENSP00000383044.2

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
RepTar	miRNAMap	SomamiR	miRcode
RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Protein 3D Structure
PDB
Disease-associated Information
GWASdb	OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	UniProt	BioGRID	mUbiSiDa
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
UBD/Alpha-Helix/UBA	UBA	23754700
UBD/Alpha-Helix/UIM	UIM	23754700
DUB/USP	USP	18538659; 19615732

Classification

Family	E-value	Score	Start	End
DUB/USP	1.00e-62	214.0	169	655
UBD/Alpha-Helix/DUIM	0.018	16.7	97	115
UBD/Alpha-Helix/UBA	0.00018	23.2	19	54

Active Site

Position(s)	Description	Evidence
178	N/A	{ECO:0000269\|PubMed:19440361}
599		{ECO:0000250}
607		{ECO:0000250}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrellle....kkkeklneenpkepkgellkslqklfeelwkskkkavsprellkalkkeaeefsgye 88
           +GlkN+gNtC++ +v+Q+L+n+ e+r+l+l+    ++ ++l+++++++++ +++++l+ lf+ l+ +k+k+v+p++ +++lk++ ++ + ++
   Q: 169  VGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNykppSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKS-NDSQ 259
           6******************************99988889999999999999*******************************99997.9*** PP
   S: 89   qqDaqEflaflldqleeelkkeknkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpvpgke 180
           qqD++Ef++ lld le+++++                  + +ee +++k k+++++lF g++    + +  +++ + +e f +++l+v+g +
   Q: 260  QQDVSEFTHKLLDWLEDAFQM------------------KAEEETDEEKPKNPMVELFYGRFLAVGVLE--GKKFENTEMFGQYPLQVNGFK 331
           *******************33..................123456788999************777664..444556899************ PP
   S: 181  etkkeslqdcLesftkeeelegenkyycekckkkqeaekklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfcts..... 267
                +l++cLe+ + e e+e+   +++e+  k+    +++++++lP+vL+++L+Rf+f+++ +++eki++k+efp+ L l++++++     
   Q: 332  -----DLHECLEAAMIEGEIES---LHSENSGKS---GQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRnreit 412
           .....9**************96...999998865...589***********************************************88888 PP
   S: 268  .........................................................................................pee 270
                                                                                                    p  
   Q: 413  rikreeikrlkdyltvlqqrlerylsygsgpkrfplvdvlqyalefasskpvctspvddidassppsgsipsqtlpstteqqgalsselP-- 502
           888888888888888888888888888888888888888877777777777777777777777777777777777665555555555550.. PP
   S: 271  kqke....................................................................................kkkY 278
              +                                                                                    ++ Y
   Q: 503  ---StspssvaaissrsvihkpftqsrippdlpmhpaprhiteeelsvlesclhrwrteiendtrdlqesisrihrtielmysdksmiQVPY 591
           ...0555555556666666666666666666666666666666666667777777777777777777788888888888888888888**** PP
   S: 279  eLiavvvHsGslssGHYvayvknekgk.WllfdDskVtevkeeevleetg.....staYvlfYk 336
           +L+av+vH+G+++ GHY+ay+ +++++ W++++D  Vt++++ee ++++      ++aY+l+Y 
   Q: 592  RLHAVLVHEGQANAGHYWAYIFDHRESrWMKYNDIAVTKSSWEELVRDSFggyrnASAYCLMYI 655
           **********************966556***************99997779************6 PP

UBD/Alpha-Helix/DUIM

   S: 1    dEdeeLqlAlalSlqEeee 19
           d+ ++Lq+A+alSl E ++
   Q: 97   DDKDDLQRAIALSLAESNR 115
           6889************875 PP

UBD/Alpha-Helix/UBA

   S: 5    dkleqlve.MGF.dreealqaLraannnleaAveyL 38
             l+ql+e +G+ d++  +qaL ++n+nle Av++L
   Q: 19   TFLNQLREiTGInDTQILQQALKDSNGNLELAVAFL 54
           569*******************************98 PP

Organism

Homo sapiens

Functional Description
(View)

Homodimer or oligomer. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels.

Protein Sequence
(Fasta)

MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDTQILQQAL KDSNGNLELA VAFLTAKNAK 60
TPQQEETTYY QTALPGNDRY ISVGSQADTN VIDLTGDDKD DLQRAIALSL AESNRAFRET 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

AAGCAGCCCG CGGACCGGCA GCAAAGGAAC GTGCGAACGC GTGACGCCGC CCGACTGGCT 60
CGCGCTCTCC CGTGCCCCGG CGTCCTCCGC CCGCTCATGG CCCGGGCCGC CGCGGACGAG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0378--Hydrolase
KW-1017--Isopeptide bond
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0645--Protease
KW-1185--Reference proteome
KW-0677--Repeat
KW-0788--Thiol protease
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR001394--Peptidase_C19_UCH
IPR009060--UBA-like
IPR003903--UIM_dom
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS50330--UIM
PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF00443--UCH
PF02809--UIM

SMART

SM00726--UIM

Gene Ontology

GO:0005829--C:cytosol
GO:0005783--C:endoplasmic reticulum
GO:0005634--C:nucleus
GO:0051117--F:ATPase binding
GO:0008233--F:peptidase activity
GO:0032183--F:SUMO binding
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0036459--F:thiol-dependent ubiquitinyl hydrolase activity
GO:0043130--F:ubiquitin binding
GO:0031625--F:ubiquitin protein ligase binding
GO:1904455--F:ubiquitin-specific protease activity involved in negative regulation of ERAD pathway
GO:0006464--P:cellular protein modification process
GO:1904293--P:negative regulation of ERAD pathway
GO:0016579--P:protein deubiquitination
GO:0071108--P:protein K48-linked deubiquitination
GO:0070536--P:protein K63-linked deubiquitination
GO:0006508--P:proteolysis
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

hsa:29761

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001340	Ailuropoda melanoleuca	94.01	0.00e+00	1976.00
IUUC-Apl-004030	Anas platyrhynchos	83.35	0.00e+00	1815.00
IUUC-Aca-004753	Anolis carolinensis	64.86	0.00e+00	992.00
IUUC-Ani-009503	Aspergillus nidulans	33.02	9.00e-11	63.20
IUUC-Ame-010624	Astyanax mexicanus	70.69	0.00e+00	1076.00
IUUC-Bta-013166	Bos taurus	96.39	0.00e+00	1969.00
IUUC-Cel-018346	Caenorhabditis elegans	28.61	2.00e-29	125.00
IUUC-Cja-019150	Callithrix jacchus	99.36	0.00e+00	1467.00
IUUC-Cfa-020649	Canis familiaris	96.79	0.00e+00	1454.00
IUUC-Cpo-021807	Cavia porcellus	93.65	0.00e+00	2056.00
IUUC-Csa-023388	Chlorocebus sabaeus	99.35	0.00e+00	1469.00
IUUC-Cho-024343	Choloepus hoffmanni	83.60	0.00e+00	970.00
IUUC-Cin-025302	Ciona intestinalis	37.50	3.00e-96	345.00
IUUC-Cgl-026575	Colletotrichum gloeosporioides	53.19	6.00e-10	60.50
IUUC-Dre-028016	Danio rerio	77.01	0.00e+00	1190.00
IUUC-Dno-029495	Dasypus novemcinctus	93.56	0.00e+00	1439.00
IUUC-Dor-030948	Dipodomys ordii	82.04	0.00e+00	1427.00
IUUC-Fca-035876	Felis catus	96.92	0.00e+00	1994.00
IUUC-Fal-036762	Ficedula albicollis	84.41	0.00e+00	1115.00
IUUC-Gmo-039490	Gadus morhua	72.52	0.00e+00	1528.00
IUUC-Gga-040817	Gallus gallus	85.22	0.00e+00	1348.00
IUUC-Gac-042473	Gasterosteus aculeatus	72.39	0.00e+00	1565.00
IUUC-Ggo-044939	Gorilla gorilla	95.12	0.00e+00	1959.00
IUUC-Kpa-049349	Komagataella pastoris	28.12	3.00e-10	61.60
IUUC-Lch-049572	Latimeria chalumnae	79.27	0.00e+00	1218.00
IUUC-Loc-052070	Lepisosteus oculatus	76.18	0.00e+00	1222.00
IUUC-Lma-053078	Leptosphaeria maculans	30.56	5.00e-12	67.40
IUUC-Laf-054426	Loxodonta africana	96.90	0.00e+00	1831.00
IUUC-Mcc-055858	Macaca mulatta	99.49	0.00e+00	1499.00
IUUC-Mga-059808	Meleagris gallopavo	85.61	0.00e+00	1827.00
IUUC-Mmr-060987	Microcebus murinus	98.07	0.00e+00	1450.00
IUUC-Mmu-064245	Mus musculus	93.66	0.00e+00	1984.00
IUUC-Mpu-066304	Mustela putorius furo	96.35	0.00e+00	1428.00
IUUC-Mlu-067139	Myotis lucifugus	96.06	0.00e+00	2001.00
IUUC-Nle-069075	Nomascus leucogenys	99.62	0.00e+00	2022.00
IUUC-Ont-072150	Oreochromis niloticus	76.14	0.00e+00	1200.00
IUUC-Oan-073054	Ornithorhynchus anatinus	80.20	0.00e+00	1690.00
IUUC-Ocu-074102	Oryctolagus cuniculus	94.30	0.00e+00	1446.00
IUUC-Ola-086529	Oryzias latipes	69.93	0.00e+00	1503.00
IUUC-Oga-088939	Otolemur garnettii	95.94	0.00e+00	1993.00
IUUC-Oar-090266	Ovis aries	97.25	0.00e+00	1430.00
IUUC-Ptr-090546	Pan troglodytes	99.62	0.00e+00	2023.00
IUUC-Pan-092311	Papio anubis	99.36	0.00e+00	1498.00
IUUC-Psi-093459	Pelodiscus sinensis	91.03	0.00e+00	1175.00
IUUC-Pfo-096726	Poecilia formosa	74.91	0.00e+00	1182.00
IUUC-Pab-097608	Pongo abelii	96.64	0.00e+00	1979.00
IUUC-Pca-100718	Procavia capensis	91.90	0.00e+00	1236.00
IUUC-Pva-103150	Pteropus vampyrus	92.60	0.00e+00	1463.00
IUUC-Rno-105727	Rattus norvegicus	94.22	0.00e+00	1953.00
IUUC-Sha-107051	Sarcophilus harrisii	89.71	0.00e+00	1858.00
IUUC-Ssl-108532	Sclerotinia sclerotiorum	28.85	8.00e-09	56.60
IUUC-Sar-113097	Sorex araneus	86.66	0.00e+00	1819.00
IUUC-Ssc-115877	Sus scrofa	94.26	0.00e+00	1961.00
IUUC-Tgu-117473	Taeniopygia guttata	78.23	0.00e+00	1682.00
IUUC-Tru-118135	Takifugu rubripes	67.46	0.00e+00	1419.00
IUUC-Tsy-119471	Tarsius syrichta	89.99	0.00e+00	1792.00
IUUC-Tni-120139	Tetraodon nigroviridis	67.71	0.00e+00	1340.00
IUUC-Tbe-127549	Tupaia belangeri	90.58	0.00e+00	1868.00
IUUC-Ttr-128896	Tursiops truncatus	90.06	0.00e+00	1890.00
IUUC-Vpa-130500	Vicugna pacos	93.58	0.00e+00	1333.00
IUUC-Xtr-132896	Xenopus tropicalis	81.17	0.00e+00	1727.00
IUUC-Xma-133349	Xiphophorus maculatus	74.72	0.00e+00	1605.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence