iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-046563

UUCD1 version

UUC-HoS-01177

Ensembl Protein ID

ENSP00000290810.3

UniProt Accession

Q13564; A6NCK0; A6NFN4; A8MU28; B2R700; B3KUP9; ULA1_HUMAN

Genbank Protein ID

AAC50477.1; AAP35030.1; BAG53511.1; BAH12735.1; BAG35647.1; AAC23784.1; CAB66732.1; EAW83042.1; AAH00480.1; AAH13301.1

Protein Name

NEDD8-activating enzyme E1 regulatory subunit; Amyloid beta precursor protein-binding protein 1, 59 kDa; Amyloid protein-binding protein 1; Proto-oncogene protein 1

Genbank Nucleotide ID

U50939; AY197612; AK097680; AK298159; AK312784; AC004638; AC044802; CH471092; BC000480; BC013301

Gene Name

NAE1; APPBP1; HPP1

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000159593.14	ENST00000562428.6	ENSP00000463546.1
ENSG00000159593.14	ENST00000359087.8	ENSP00000351990.4
ENSG00000159593.14	ENST00000290810.7	ENSP00000290810.3
ENSG00000159593.14	ENST00000566663.5	ENSP00000462937.2
ENSG00000159593.14	ENST00000379463.6	ENSP00000368776.2
ENSG00000159593.14	ENST00000567743.5	ENSP00000455562.1
ENSG00000159593.14	ENST00000563253.5	ENSP00000454471.1
ENSG00000159593.14	ENST00000563185.6	ENSP00000454506.2
ENSG00000159593.14	ENST00000569388.5	ENSP00000456847.1
ENSG00000159593.14	ENST00000565535.5	ENSP00000455967.1
ENSG00000159593.14	ENST00000569348.5	ENSP00000456564.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	circBase	circRNADb	CircNet
miRTarBase	microRNA	TRANSFAC	miRWalk
RepTar	miRNAMap	SomamiR	miRcode
RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Protein 3D Structure
PDB	MMDB	SCOP
Disease-associated Information
GWASdb	OMIM
Drug and target
DrugBank	Manually
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	UniProt	BioGRID	mUbiSiDa
DNA Methylation
TCGA	ICGC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E1	E1_ThiF	12740388; 19509147

Classification

Family	E-value	Score	Start	End
E1	4.10e-134	449.5	13	525

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnvea 92
           YDRQ+rlwG+++qe+l+sa+v+l++a+++G+E+lKnLvl G+gs+t++D+++v+ ++ +++F+++++++gk++Ae+a+e+l+eln+dv++++
   Q: 13   YDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSF 104
           ******************************************************************************************** PP
   S: 93   heesiteleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketp 180
           +ees+++l ++++ Ff++f+vVv+++ +++++++++++++n ++pl++++t+Gl+G++r+iik++++++s++d   +++++++Pf++l+e++
   Q: 105  VEESPENLLDNDPsFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDnalEDLRLDKPFPELREHF 196
           *******************************************************************************************9 PP
   S: 181  n.........aaehtiewavlfnklleeeageeevlekldseekeegkdkvkselks......edeenfeeaieiavkafakttins.ikql 256
           +         +++++++w+v+++k+l+++++e++++ +++++eke+++d++++++ +      edeenfeeai+++++a+++t+i+s i+++
   Q: 197  QsydldhmekKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKnengapEDEENFEEAIKNVNTALNTTQIPSsIEDI 288
           9999999998***********************************************9********************************** PP
   S: 257  lksfecdivtkskspfwv.......................................................................... 274
           +++++c ++tk++++fw+                                                                          
   Q: 289  FNDDRCINITKQTPSFWIlaralkefvakegqgnlpvrgtipdmiadsgkyiklqnvyrekakkdaaavgnhvakllqsigqapesisekel 380
           ******************************************************************************************** PP
   S: 275  .sfcsnaealqvp...........ekvekdeevvkasap.....lylllralerfekkkgrkpgelsfekddds..avdlvtaaanlraesl 347
             +csn++ l+v            ++++kde+++++++p     lyl+lra++rf+k++gr+pg++++++++d+  + +++t ++    +++
   Q: 381  kLLCSNSAFLRVVrcrslaeeyglDTINKDEIISSMDNPdneivLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIgkLKSCLTGFL----QEY 468
           *99********7777777888888**************9***********************************999********....*** PP
   S: 348  giepkldddlikeiagniipaiattnavvgGlaaqEviKvvsgkfeplknfflfdae 404
           g++ +++dd+++e++++++++ +t++a++gG+aaqEviK+++++f+ ++n++++ ++
   Q: 469  GLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGM 525
           *****************************************************9776 PP

Organism

Homo sapiens

Functional Description
(View)

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Protein Sequence
(Fasta)

MAQLGKLLKE QKYDRQLRLW GDHGQEALES AHVCLINATA TGTEILKNLV LPGIGSFTII 60
DGNQVSGEDA GNNFFLQRSS IGKNRAEAAM EFLQELNSDV SGSFVEESPE NLLDNDPSFF 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CGCAGAGTCG GCTGCGTCAG CAGGCAATGG CCTTGGAGCA CCGGCCTGAG CTACCCAGGG 60
AGACGGTGGA TCTCCAGGAA ACCGCGAGTG CCTAGGAGCC CCTCCCCACC TTTTCCGGGG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0053--Apoptosis
KW-0131--Cell cycle
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0472--Membrane
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005886--C:plasma membrane
GO:0019781--F:NEDD8 activating enzyme activity
GO:0046982--F:protein heterodimerization activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0033314--P:mitotic DNA replication checkpoint
GO:0051402--P:neuron apoptotic process
GO:0045116--P:protein neddylation
GO:0042981--P:regulation of apoptotic process
GO:0043523--P:regulation of neuron apoptotic process
GO:0007165--P:signal transduction

KEGG

hsa:8883

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000764	Aegilops tauschii	42.53	2.00e-125	442.00
IUUC-Aml-002333	Ailuropoda melanoleuca	97.20	0.00e+00	1083.00
IUUC-Atr-002644	Amborella trichopoda	41.27	1.00e-113	403.00
IUUC-Apl-003446	Anas platyrhynchos	83.82	0.00e+00	917.00
IUUC-Aca-004450	Anolis carolinensis	80.11	0.00e+00	915.00
IUUC-Aly-006187	Arabidopsis lyrata	40.46	2.00e-124	439.00
IUUC-Ath-007600	Arabidopsis thaliana	41.03	8.00e-126	444.00
IUUC-Ago-007900	Ashbya gossypii	27.07	7.00e-40	158.00
IUUC-Acl-008333	Aspergillus clavatus	34.87	4.00e-82	299.00
IUUC-Afl-008529	Aspergillus flavus	36.54	4.00e-87	315.00
IUUC-Afu-009071	Aspergillus fumigatus	33.87	2.00e-82	300.00
IUUC-Ani-009443	Aspergillus nidulans	34.05	5.00e-80	291.00
IUUC-Ang-009669	Aspergillus niger	33.69	3.00e-83	302.00
IUUC-Aor-009914	Aspergillus oryzae	35.87	7.00e-84	305.00
IUUC-Ate-010450	Aspergillus terreus	34.88	4.00e-85	308.00
IUUC-Ame-011183	Astyanax mexicanus	72.02	0.00e+00	830.00
IUUC-Bgr-012111	Blumeria graminis	33.33	4.00e-67	249.00
IUUC-Bta-012583	Bos taurus	97.75	0.00e+00	1090.00
IUUC-Bci-013892	Botrytis cinerea	33.39	9.00e-66	244.00
IUUC-Bdi-014592	Brachypodium distachyon	43.32	6.00e-128	451.00
IUUC-Bol-016399	Brassica oleracea	40.08	4.00e-123	434.00
IUUC-Bra-017506	Brassica rapa	39.05	1.00e-118	420.00
IUUC-Cel-018456	Caenorhabditis elegans	36.16	1.00e-94	340.00
IUUC-Cja-020007	Callithrix jacchus	97.95	0.00e+00	1092.00
IUUC-Cfa-020324	Canis familiaris	97.75	0.00e+00	1088.00
IUUC-Cpo-022248	Cavia porcellus	66.54	0.00e+00	643.00
IUUC-Csa-023149	Chlorocebus sabaeus	99.34	0.00e+00	936.00
IUUC-Cho-024537	Choloepus hoffmanni	85.47	0.00e+00	808.00
IUUC-Cin-025763	Ciona intestinalis	49.53	2.00e-156	545.00
IUUC-Csv-026113	Ciona savignyi	46.67	1.00e-146	513.00
IUUC-Cgl-026428	Colletotrichum gloeosporioides	31.64	2.00e-65	243.00
IUUC-Cne-026884	Cryptococcus neoformans	30.07	2.00e-72	267.00
IUUC-Cme-027210	Cyanidioschyzon merolae	27.11	3.00e-19	90.50
IUUC-Dre-028140	Danio rerio	73.35	0.00e+00	850.00
IUUC-Dno-030033	Dasypus novemcinctus	97.89	0.00e+00	966.00
IUUC-Dor-030513	Dipodomys ordii	68.69	3.00e-115	408.00
IUUC-Dse-031536	Dothistroma septosporum	35.47	7.00e-86	311.00
IUUC-Dme-031683	Drosophila melanogaster	44.47	2.00e-127	449.00
IUUC-Ete-032420	Echinops telfairi	83.14	0.00e+00	887.00
IUUC-Eca-033992	Equus caballus	97.98	0.00e+00	917.00
IUUC-Eeu-034713	Erinaceus europaeus	79.92	1.00e-117	417.00
IUUC-Fca-036548	Felis catus	96.63	0.00e+00	1078.00
IUUC-Fal-037600	Ficedula albicollis	80.65	0.00e+00	886.00
IUUC-Fox-038037	Fusarium oxysporum	33.94	2.00e-77	283.00
IUUC-Fso-038387	Fusarium solani	34.77	5.00e-79	288.00
IUUC-Gmo-038562	Gadus morhua	71.21	0.00e+00	823.00
IUUC-Ggr-040005	Gaeumannomyces graminis	34.48	4.00e-74	272.00
IUUC-Gga-041129	Gallus gallus	84.11	0.00e+00	937.00
IUUC-Gac-042558	Gasterosteus aculeatus	72.50	0.00e+00	833.00
IUUC-Gma-043610	Glycine max	41.44	6.00e-120	424.00
IUUC-Ggo-045611	Gorilla gorilla	99.07	0.00e+00	1103.00
IUUC-Hvu-047790	Hordeum vulgare	38.11	9.00e-77	280.00
IUUC-Itr-048066	Ictidomys tridecemlineatus	97.94	0.00e+00	1091.00
IUUC-Kpa-049309	Komagataella pastoris	35.55	2.00e-82	299.00
IUUC-Lch-050038	Latimeria chalumnae	76.42	0.00e+00	856.00
IUUC-Lpe-051535	Leersia perrieri	41.45	5.00e-111	394.00
IUUC-Loc-051917	Lepisosteus oculatus	69.37	0.00e+00	794.00
IUUC-Lma-053112	Leptosphaeria maculans	35.69	5.00e-87	315.00
IUUC-Laf-053690	Loxodonta africana	96.44	0.00e+00	1082.00
IUUC-Mcc-055117	Macaca mulatta	97.03	0.00e+00	1078.00
IUUC-Meu-056761	Macropus eugenii	77.18	0.00e+00	827.00
IUUC-Mor-057230	Magnaporthe oryzae	33.63	3.00e-78	285.00
IUUC-Mpo-057514	Magnaporthe poae	33.63	8.00e-72	264.00
IUUC-Mtr-058551	Medicago truncatula	40.74	7.00e-117	414.00
IUUC-Mla-059183	Melampsora laricipopulina	36.80	7.00e-96	344.00
IUUC-Mga-060144	Meleagris gallopavo	82.95	0.00e+00	915.00
IUUC-Mvi-060221	Microbotryum violaceum	35.81	3.00e-82	299.00
IUUC-Mmr-061325	Microcebus murinus	94.97	0.00e+00	1059.00
IUUC-Mdo-062532	Monodelphis domestica	91.74	0.00e+00	1030.00
IUUC-Mmu-063956	Mus musculus	97.00	0.00e+00	1083.00
IUUC-Mac-064521	Musa acuminata	40.75	4.00e-123	435.00
IUUC-Mpu-066716	Mustela putorius furo	96.81	0.00e+00	1021.00
IUUC-Mlu-067459	Myotis lucifugus	95.87	0.00e+00	1063.00
IUUC-Nfi-068245	Neosartorya fischeri	34.22	2.00e-82	300.00
IUUC-Ncr-068910	Neurospora crassa	33.88	6.00e-77	281.00
IUUC-Nle-070034	Nomascus leucogenys	96.38	0.00e+00	1050.00
IUUC-Opr-070552	Ochotona princeps	87.43	0.00e+00	886.00
IUUC-Ont-071426	Oreochromis niloticus	73.16	0.00e+00	851.00
IUUC-Oan-073389	Ornithorhynchus anatinus	83.60	0.00e+00	776.00
IUUC-Ocu-074911	Oryctolagus cuniculus	96.25	0.00e+00	1082.00
IUUC-Oba-075437	Oryza barthii	42.94	4.00e-118	418.00
IUUC-Obr-076684	Oryza brachyantha	42.97	2.00e-113	402.00
IUUC-Ogl-077170	Oryza glaberrima	42.94	4.00e-118	418.00
IUUC-Ogu-078844	Oryza glumaepatula	43.71	4.00e-111	395.00
IUUC-Oin-079105	Oryza indica	42.94	1.00e-117	416.00
IUUC-Olo-080943	Oryza longistaminata	40.44	5.00e-106	378.00
IUUC-Oni-082891	Oryza nivara	40.08	2.00e-111	396.00
IUUC-Opu-083457	Oryza punctata	42.94	1.00e-117	416.00
IUUC-Oru-084960	Oryza rufipogon	40.44	5.00e-106	378.00
IUUC-Osa-085358	Oryza sativa	43.13	4.00e-118	418.00
IUUC-Ola-086420	Oryzias latipes	71.05	7.00e-168	583.00
IUUC-Olu-087625	Ostreococcus lucimarinus	30.85	2.00e-60	227.00
IUUC-Oga-088810	Otolemur garnettii	96.07	0.00e+00	1077.00
IUUC-Oar-089196	Ovis aries	97.21	0.00e+00	1087.00
IUUC-Ptr-090617	Pan troglodytes	99.63	0.00e+00	1107.00
IUUC-Pan-092761	Papio anubis	99.06	0.00e+00	1100.00
IUUC-Psi-093191	Pelodiscus sinensis	86.65	0.00e+00	963.00
IUUC-Pma-094380	Petromyzon marinus	62.07	0.00e+00	663.00
IUUC-Pno-095084	Phaeosphaeria nodorum	32.79	3.00e-74	274.00
IUUC-Ppa-095875	Physcomitrella patens	40.04	4.00e-119	421.00
IUUC-Pfo-096467	Poecilia formosa	72.40	0.00e+00	833.00
IUUC-Pab-098255	Pongo abelii	98.65	0.00e+00	914.00
IUUC-Pop-099722	Populus trichocarpa	41.03	6.00e-124	437.00
IUUC-Pca-100807	Procavia capensis	78.87	3.00e-118	418.00
IUUC-Ppe-101280	Prunus persica	40.68	8.00e-123	434.00
IUUC-Pva-102333	Pteropus vampyrus	92.74	0.00e+00	1023.00
IUUC-Pgr-103401	Puccinia graminis	32.30	2.00e-81	296.00
IUUC-Ptt-103889	Puccinia triticina	27.67	1.00e-58	221.00
IUUC-Pte-104355	Pyrenophora teres	36.77	1.00e-90	327.00
IUUC-Pyt-104545	Pyrenophora triticirepentis	36.19	4.00e-91	328.00
IUUC-Rno-105810	Rattus norvegicus	96.44	0.00e+00	1078.00
IUUC-Sce-106397	Saccharomyces cerevisiae	26.02	4.00e-37	149.00
IUUC-Sha-106770	Sarcophilus harrisii	90.11	2.00e-154	537.00
IUUC-Sja-107926	Schizosaccharomyces japonicus	33.14	1.00e-77	283.00
IUUC-Spo-108276	Schizosaccharomyces pombe	34.83	1.00e-82	300.00
IUUC-Ssl-108387	Sclerotinia sclerotiorum	32.55	6.00e-68	251.00
IUUC-Smo-109296	Selaginella moellendorffii	42.42	7.00e-123	434.00
IUUC-Sit-110157	Setaria italica	44.40	1.00e-134	473.00
IUUC-Sly-111105	Solanum lycopersicum	40.19	2.00e-121	429.00
IUUC-Stu-112469	Solanum tuberosum	40.71	2.00e-123	436.00
IUUC-Sar-113083	Sorex araneus	73.26	0.00e+00	752.00
IUUC-Sbi-113813	Sorghum bicolor	44.40	1.00e-133	470.00
IUUC-Sre-115058	Sporisorium reilianum	38.89	4.00e-85	309.00
IUUC-Tgu-116772	Taeniopygia guttata	80.27	0.00e+00	885.00
IUUC-Tru-118686	Takifugu rubripes	71.46	0.00e+00	834.00
IUUC-Tsy-119111	Tarsius syrichta	89.05	5.00e-149	521.00
IUUC-Tni-120231	Tetraodon nigroviridis	66.23	0.00e+00	751.00
IUUC-Tca-121491	Theobroma cacao	40.72	3.00e-116	412.00
IUUC-Tre-122103	Trichoderma reesei	37.00	9.00e-84	304.00
IUUC-Tvi-122672	Trichoderma virens	36.88	8.00e-76	278.00
IUUC-Tae-125679	Triticum aestivum	43.13	1.00e-127	450.00
IUUC-Tur-126487	Triticum urartu	41.41	6.00e-121	427.00
IUUC-Tme-127097	Tuber melanosporum	38.73	5.00e-94	338.00
IUUC-Ttr-128465	Tursiops truncatus	90.13	0.00e+00	995.00
IUUC-Uma-129645	Ustilago maydis	33.83	1.00e-90	327.00
IUUC-Vda-129989	Verticillium dahliae	30.68	2.00e-56	213.00
IUUC-Vpa-130529	Vicugna pacos	92.74	0.00e+00	1030.00
IUUC-Vvi-131059	Vitis vinifera	41.63	2.00e-121	429.00
IUUC-Xtr-132002	Xenopus tropicalis	78.75	0.00e+00	906.00
IUUC-Xma-133930	Xiphophorus maculatus	72.81	0.00e+00	838.00
IUUC-Yli-134505	Yarrowia lipolytica	35.05	3.00e-86	312.00
IUUC-Zma-135895	Zea mays	44.66	6.00e-133	468.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence