iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Rno-105233

UUCD1 version

UUC-RaN-01036

Ensembl Protein ID

ENSRNOP00000028752.5

UniProt Accession

B2RYG6; OTUB1_RAT

Genbank Protein ID

EDM12666.1; AAI66771.1

Protein Name

Ubiquitin thioesterase OTUB1; Deubiquitinating enzyme OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Otubain-1; Ubiquitin-specific-processing protease OTUB1

Genbank Nucleotide ID

CH473953; BC166771

Gene Name

Otub1

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSRNOG00000021175.5	ENSRNOT00000028752.5	ENSRNOP00000028752.5

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
DNA & RNA Element
TRANSFAC	RAID2
Protein-protein Interaction
IID	iRefIndex	HINT
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/OTU	3.10e-51	173.8	81	271

Active Site

Position(s)	Description	Evidence
88	N/A	{ECO:0000250\|UniProtKB:Q96FW1}
91	Nucleophile	{ECO:0000250\|UniProtKB:Q96FW1}
265		{ECO:0000250\|UniProtKB:Q96FW1}

Domain Profile

DUB/OTU

   S: 2    lilktkgDGnCLlrAvsqs..........................................................llgvfdddslsd 32
           +i+kt++DGnC++rA+++s                                                          ll++f+d+s+sd
   Q: 81   YIRKTRPDGNCFYRAFGFShleallddskelqrfkavsakskedlvsqgfteftiedfhntfmdlieqvekqtsvadLLASFNDQSTSD 169
           89*************************************************************************************** PP
   S: 33   alveaLreltseylqkesdffsefieekedekefcqklvepeak.geeihifaLaqiLsrpiivvdvdsleskvggillpllfpeeklk 120
           +lv++Lr+lts ylq+es+ff++fie+++++kefcq++vep++k +++ihi+aLaq+Ls++i+v+++d++e   gg+++p++fpe + +
   Q: 170  YLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKeSDHIHIIALAQALSVSIQVEYMDRGE---GGTTNPHVFPEGS-E 254
           **********************************************************************9...9************.* PP
   S: 121  kpivLlYrsgHYdaLva 137
           ++++LlYr+gHYd+L++
   Q: 255  PKVYLLYRPGHYDILYK 271
           ***************96 PP

Organism

Rattus norvegicus

Functional Description
(View)

Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain (By similarity).

Protein Sequence
(Fasta)

MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE 60
YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATCGCCAGTG CAGCGCTGTT TAAAGATGGC GGCGGAGGAA CCTCAGCAGC AGAAGCAGGA 60
GCCGCTGGGC AGCGATTCCG AAGGTACGAA CGGAGGGAAG GATGTTCTGC CCGGGGTAGC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-1064--Adaptive immunity
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0227--DNA damage
KW-0234--DNA repair
KW-0378--Hydrolase
KW-0391--Immunity
KW-0597--Phosphoprotein
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0833--Ubl conjugation pathway

Interpro

IPR003323--OTU_dom
IPR030298--OTUB1
IPR016615--Otubain
IPR019400--Peptidase_C65_otubain

PROSITE

PS50802--OTU

Pfam

PF10275--Peptidase_C65

Gene Ontology

GO:0005737--C:cytoplasm
GO:0070062--C:extracellular exosome
GO:0005634--C:nucleus
GO:0019784--F:NEDD8-specific protease activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0043130--F:ubiquitin binding
GO:0002250--P:adaptive immune response
GO:0006974--P:cellular response to DNA damage stimulus
GO:0071347--P:cellular response to interleukin-1
GO:0006281--P:DNA repair
GO:2000780--P:negative regulation of double-strand break repair
GO:1901315--P:negative regulation of histone H2A K63-linked ubiquitination
GO:0071108--P:protein K48-linked deubiquitination

KEGG

rno:293705

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001871	Ailuropoda melanoleuca	97.80	9.00e-156	542.00
IUUC-Apl-003233	Anas platyrhynchos	58.46	5.00e-19	85.10
IUUC-Aca-004648	Anolis carolinensis	85.77	1.00e-137	481.00
IUUC-Aly-005710	Arabidopsis lyrata	42.12	5.00e-52	197.00
IUUC-Ath-007112	Arabidopsis thaliana	41.97	6.00e-51	194.00
IUUC-Afl-008439	Aspergillus flavus	34.18	2.00e-08	52.40
IUUC-Ame-010662	Astyanax mexicanus	83.27	2.00e-130	457.00
IUUC-Bta-013533	Bos taurus	99.26	5.00e-153	533.00
IUUC-Bol-016100	Brassica oleracea	42.05	2.00e-50	192.00
IUUC-Bra-017705	Brassica rapa	39.15	3.00e-45	175.00
IUUC-Cel-018295	Caenorhabditis elegans	34.42	6.00e-42	164.00
IUUC-Cja-019190	Callithrix jacchus	99.63	4.00e-154	536.00
IUUC-Cfa-020430	Canis familiaris	99.60	1.00e-149	521.00
IUUC-Cpo-022355	Cavia porcellus	100.00	3.00e-154	537.00
IUUC-Cre-022997	Chlamydomonas reinhardtii	38.80	8.00e-47	180.00
IUUC-Csa-023286	Chlorocebus sabaeus	96.43	1.00e-151	528.00
IUUC-Cin-025507	Ciona intestinalis	43.33	2.00e-44	172.00
IUUC-Csv-026273	Ciona savignyi	49.80	5.00e-69	254.00
IUUC-Dre-027843	Danio rerio	78.95	1.00e-126	445.00
IUUC-Dno-029165	Dasypus novemcinctus	98.50	1.00e-117	415.00
IUUC-Dor-030943	Dipodomys ordii	100.00	3.00e-154	537.00
IUUC-Dme-031922	Drosophila melanogaster	56.40	2.00e-78	285.00
IUUC-Ete-032296	Echinops telfairi	96.86	9.00e-90	323.00
IUUC-Eca-033695	Equus caballus	98.41	5.00e-148	516.00
IUUC-Fca-036441	Felis catus	99.60	1.00e-149	521.00
IUUC-Gmo-038908	Gadus morhua	80.68	8.00e-127	446.00
IUUC-Ggr-040020	Gaeumannomyces graminis	31.41	9.00e-33	135.00
IUUC-Gga-040460	Gallus gallus	81.92	4.00e-134	470.00
IUUC-Gac-042417	Gasterosteus aculeatus	81.60	8.00e-123	432.00
IUUC-Gma-043712	Glycine max	41.13	1.00e-51	196.00
IUUC-Ggo-044552	Gorilla gorilla	99.37	1.00e-92	332.00
IUUC-Hsa-046766	Homo sapiens	99.63	4.00e-154	536.00
IUUC-Itr-048312	Ictidomys tridecemlineatus	100.00	3.00e-154	537.00
IUUC-Lch-050602	Latimeria chalumnae	80.95	4.00e-119	420.00
IUUC-Laf-053705	Loxodonta africana	100.00	3.00e-154	537.00
IUUC-Mcc-055745	Macaca mulatta	93.43	1.00e-150	525.00
IUUC-Meu-056569	Macropus eugenii	85.98	1.00e-135	474.00
IUUC-Mtr-057712	Medicago truncatula	43.49	9.00e-47	180.00
IUUC-Mvi-060358	Microbotryum violaceum	33.94	2.00e-15	75.90
IUUC-Mmr-061123	Microcebus murinus	99.57	7.00e-128	449.00
IUUC-Mdo-062613	Monodelphis domestica	87.45	9.00e-124	436.00
IUUC-Mmu-063658	Mus musculus	100.00	3.00e-154	537.00
IUUC-Mac-065274	Musa acuminata	38.00	1.00e-39	156.00
IUUC-Mpu-066172	Mustela putorius furo	99.63	4.00e-154	536.00
IUUC-Mlu-066966	Myotis lucifugus	98.89	4.00e-153	533.00
IUUC-Nle-069519	Nomascus leucogenys	87.63	2.00e-93	335.00
IUUC-Ont-071901	Oreochromis niloticus	82.87	3.00e-125	441.00
IUUC-Ola-087331	Oryzias latipes	81.46	3.00e-86	310.00
IUUC-Oga-088064	Otolemur garnettii	99.63	4.00e-154	536.00
IUUC-Oar-089251	Ovis aries	98.43	6.00e-148	516.00
IUUC-Ptr-091430	Pan troglodytes	99.63	6.00e-154	536.00
IUUC-Psi-093823	Pelodiscus sinensis	88.12	3.00e-134	470.00
IUUC-Pma-094366	Petromyzon marinus	72.01	3.00e-103	367.00
IUUC-Pfo-096734	Poecilia formosa	80.48	5.00e-114	403.00
IUUC-Pab-097919	Pongo abelii	100.00	3.00e-127	447.00
IUUC-Pop-099401	Populus trichocarpa	41.24	1.00e-48	186.00
IUUC-Ppe-101977	Prunus persica	42.38	1.00e-51	196.00
IUUC-Pva-102499	Pteropus vampyrus	99.60	4.00e-149	520.00
IUUC-Sha-107081	Sarcophilus harrisii	96.90	9.00e-149	519.00
IUUC-Sly-111640	Solanum lycopersicum	42.01	3.00e-49	188.00
IUUC-Ssc-116202	Sus scrofa	99.63	4.00e-154	536.00
IUUC-Tru-117664	Takifugu rubripes	77.70	5.00e-123	433.00
IUUC-Tca-121467	Theobroma cacao	41.54	2.00e-50	192.00
IUUC-Tbe-128116	Tupaia belangeri	98.14	2.00e-93	335.00
IUUC-Ttr-128622	Tursiops truncatus	98.89	2.00e-151	528.00
IUUC-Vvi-131389	Vitis vinifera	43.75	5.00e-53	201.00
IUUC-Xma-134191	Xiphophorus maculatus	80.88	5.00e-115	407.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence