iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

mRNA Expression
GEO	FFGED
Protein-protein Interaction
IID	iRefIndex	HINT	Mentha
Post-translational Modifications (PTMs)
BioGRID
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E1	E1_ThiF	9545234; 10993680

Classification

Family	E-value	Score	Start	End
E1	8.80e-81	270.6	3	184

Active Site

Position(s)	Description	Evidence
168	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU10132}

Domain Profile

   S: 19   akvllvGagglGcEllKnLvlaGv.gsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesiteleeeivFfkkfdvVvlaldn 119
           +k+l++GagglGcE+lKnL+++ + +++++vD dt+e++nlnrqFLf+++d+gk+KA+vaa+ ++ ++p+ +v+ah +++t+l  +  F+k+f++++++ld 
   Q: 3    CKILVLGAGGLGCEILKNLTMLSFvKQVHIVDIDTIELTNLNRQFLFCDKDIGKPKAQVAAQYVNTRFPQLEVVAHVQDLTTLPPS--FYKDFQFIISGLDA 102
           89*********************99*************************************************************..************** PP
   S: 120  rearrkvnrlclna........rvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpnaaehtiewa 190
           +e rr +n+ ++++        ++p+i++gt+Gl+G+v++ii+g+t+c++cs    +++q+t+P+ct++++p+++eh++e++
   Q: 103  IEPRRFINETLVKLtlesnyeiCIPFIDGGTEGLKGHVKTIIPGITACWECSIdtlPSQQDTVPMCTIANNPRCIEHVVEYV 184
           *****************************************************9***************************9 PP

Organism

Saccharomyces cerevisiae

Functional Description
(View)

Catalytic subunit of the dimeric UBA3-ULA1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBC12.

Protein Sequence
(Fasta)

MDCKILVLGA GGLGCEILKN LTMLSFVKQV HIVDIDTIEL TNLNRQFLFC DKDIGKPKAQ 60
VAAQYVNTRF PQLEVVAHVQ DLTTLPPSFY KDFQFIISGL DAIEPRRFIN ETLVKLTLES 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGGACTGCA AGATATTGGT GTTAGGCGCA GGTGGTCTAG GATGCGAAAT CCTGAAGAAT 60
TTGACAATGT TAAGCTTTGT TAAACAGGTT CATATTGTAG ATATAGACAC AATCGAGCTA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR030468--Uba3
IPR033127--UBQ-activ_enz_E1_Cys_AS

PROSITE

PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF00899--ThiF

Gene Ontology

GO:0016881--F:acid-amino acid ligase activity
GO:0005524--F:ATP binding
GO:0019781--F:NEDD8 activating enzyme activity
GO:0045116--P:protein neddylation

KEGG

sce:YPR066W

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001124	Aegilops tauschii	36.53	1.00e-39	155.00
IUUC-Aml-001578	Ailuropoda melanoleuca	42.36	9.00e-71	258.00
IUUC-Atr-002591	Amborella trichopoda	36.24	1.00e-41	162.00
IUUC-Apl-003671	Anas platyrhynchos	43.09	5.00e-72	263.00
IUUC-Aca-004749	Anolis carolinensis	44.16	9.00e-74	268.00
IUUC-Aly-005747	Arabidopsis lyrata	40.07	1.00e-52	198.00
IUUC-Ath-006623	Arabidopsis thaliana	39.74	2.00e-52	197.00
IUUC-Ago-007934	Ashbya gossypii	51.13	2.00e-89	320.00
IUUC-Acl-008047	Aspergillus clavatus	39.60	4.00e-62	229.00
IUUC-Afl-008615	Aspergillus flavus	32.42	2.00e-33	134.00
IUUC-Afu-008996	Aspergillus fumigatus	39.26	3.00e-56	210.00
IUUC-Ani-009281	Aspergillus nidulans	37.07	7.00e-35	139.00
IUUC-Ang-009539	Aspergillus niger	39.93	2.00e-52	197.00
IUUC-Aor-010151	Aspergillus oryzae	37.30	1.00e-50	191.00
IUUC-Ate-010502	Aspergillus terreus	40.53	2.00e-54	204.00
IUUC-Ame-011837	Astyanax mexicanus	43.65	4.00e-65	239.00
IUUC-Bgr-012068	Blumeria graminis	40.00	3.00e-54	203.00
IUUC-Bta-013003	Bos taurus	42.68	1.00e-71	261.00
IUUC-Bci-013983	Botrytis cinerea	40.53	1.00e-57	214.00
IUUC-Bdi-014429	Brachypodium distachyon	35.89	3.00e-41	160.00
IUUC-Bol-015813	Brassica oleracea	36.05	5.00e-43	166.00
IUUC-Bra-018023	Brassica rapa	36.46	2.00e-42	164.00
IUUC-Cel-018684	Caenorhabditis elegans	38.71	3.00e-59	220.00
IUUC-Cja-019200	Callithrix jacchus	43.22	1.00e-70	258.00
IUUC-Cfa-021038	Canis familiaris	42.68	2.00e-71	260.00
IUUC-Cre-022778	Chlamydomonas reinhardtii	38.56	1.00e-61	228.00
IUUC-Csa-024079	Chlorocebus sabaeus	42.68	1.00e-71	261.00
IUUC-Cho-025052	Choloepus hoffmanni	45.65	2.00e-57	214.00
IUUC-Cin-025113	Ciona intestinalis	40.38	6.00e-57	212.00
IUUC-Csv-026231	Ciona savignyi	40.58	6.00e-64	236.00
IUUC-Cgl-026535	Colletotrichum gloeosporioides	41.06	5.00e-59	219.00
IUUC-Cne-026850	Cryptococcus neoformans	42.52	6.00e-63	232.00
IUUC-Cme-027275	Cyanidioschyzon merolae	31.55	8.00e-47	179.00
IUUC-Dre-028751	Danio rerio	42.02	4.00e-63	233.00
IUUC-Dno-029571	Dasypus novemcinctus	42.68	3.00e-71	260.00
IUUC-Dor-030395	Dipodomys ordii	42.36	3.00e-71	260.00
IUUC-Dse-031329	Dothistroma septosporum	36.22	5.00e-52	196.00
IUUC-Dme-031776	Drosophila melanogaster	39.87	4.00e-59	219.00
IUUC-Ete-032894	Echinops telfairi	40.96	2.00e-31	127.00
IUUC-Eca-034389	Equus caballus	42.68	2.00e-71	261.00
IUUC-Eeu-035152	Erinaceus europaeus	39.49	6.00e-60	222.00
IUUC-Fca-036032	Felis catus	42.68	2.00e-71	261.00
IUUC-Fal-037506	Ficedula albicollis	43.31	6.00e-72	262.00
IUUC-Fox-037871	Fusarium oxysporum	38.57	1.00e-48	184.00
IUUC-Fso-038350	Fusarium solani	42.67	3.00e-58	217.00
IUUC-Gmo-039246	Gadus morhua	44.12	2.00e-64	237.00
IUUC-Ggr-039859	Gaeumannomyces graminis	41.28	3.00e-60	223.00
IUUC-Gga-040843	Gallus gallus	42.12	4.00e-67	246.00
IUUC-Gac-042610	Gasterosteus aculeatus	43.04	3.00e-64	237.00
IUUC-Gma-043031	Glycine max	36.59	8.00e-44	169.00
IUUC-Ggo-044788	Gorilla gorilla	42.86	1.00e-70	258.00
IUUC-Hsa-045849	Homo sapiens	42.99	9.00e-72	261.00
IUUC-Hvu-047819	Hordeum vulgare	36.59	3.00e-42	163.00
IUUC-Itr-047863	Ictidomys tridecemlineatus	42.36	2.00e-71	261.00
IUUC-Kpa-049393	Komagataella pastoris	43.71	3.00e-66	243.00
IUUC-Lch-049471	Latimeria chalumnae	43.55	4.00e-65	239.00
IUUC-Lpe-051297	Leersia perrieri	35.89	5.00e-48	183.00
IUUC-Loc-052910	Lepisosteus oculatus	43.69	1.00e-64	238.00
IUUC-Lma-053181	Leptosphaeria maculans	39.02	2.00e-61	227.00
IUUC-Laf-054314	Loxodonta africana	42.01	1.00e-69	255.00
IUUC-Mcc-054870	Macaca mulatta	42.99	9.00e-72	261.00
IUUC-Mor-056919	Magnaporthe oryzae	40.27	4.00e-58	216.00
IUUC-Mpo-057614	Magnaporthe poae	41.61	6.00e-58	216.00
IUUC-Mtr-058110	Medicago truncatula	37.23	1.00e-43	168.00
IUUC-Mla-058949	Melampsora laricipopulina	43.19	1.00e-64	238.00
IUUC-Mga-059455	Meleagris gallopavo	42.77	2.00e-71	261.00
IUUC-Mvi-060270	Microbotryum violaceum	38.74	1.00e-58	218.00
IUUC-Mmr-061155	Microcebus murinus	42.04	5.00e-70	256.00
IUUC-Mdo-062037	Monodelphis domestica	42.68	8.00e-71	258.00
IUUC-Mmu-063670	Mus musculus	42.68	1.00e-71	261.00
IUUC-Mac-064711	Musa acuminata	35.42	1.00e-39	155.00
IUUC-Mpu-066734	Mustela putorius furo	42.41	3.00e-71	259.00
IUUC-Mlu-067068	Myotis lucifugus	42.36	6.00e-71	259.00
IUUC-Nfi-068349	Neosartorya fischeri	39.93	1.00e-57	215.00
IUUC-Ncr-068626	Neurospora crassa	39.40	6.00e-55	206.00
IUUC-Nle-069366	Nomascus leucogenys	42.99	4.00e-71	259.00
IUUC-Ont-072196	Oreochromis niloticus	42.72	3.00e-62	230.00
IUUC-Oan-073511	Ornithorhynchus anatinus	41.94	3.00e-69	253.00
IUUC-Ocu-073773	Oryctolagus cuniculus	42.99	6.00e-72	262.00
IUUC-Oba-075726	Oryza barthii	35.79	2.00e-45	174.00
IUUC-Obr-076286	Oryza brachyantha	35.89	3.00e-48	184.00
IUUC-Ogu-078473	Oryza glumaepatula	36.05	2.00e-45	174.00
IUUC-Oin-079393	Oryza indica	36.24	6.00e-49	186.00
IUUC-Olo-080446	Oryza longistaminata	36.65	1.00e-46	178.00
IUUC-Ome-081737	Oryza meridionalis	36.65	1.00e-46	178.00
IUUC-Oni-082853	Oryza nivara	35.79	2.00e-45	174.00
IUUC-Opu-083590	Oryza punctata	36.24	7.00e-49	186.00
IUUC-Oru-084184	Oryza rufipogon	35.79	2.00e-45	174.00
IUUC-Osa-085402	Oryza sativa	36.24	6.00e-49	186.00
IUUC-Ola-086671	Oryzias latipes	41.83	7.00e-57	212.00
IUUC-Olu-087738	Ostreococcus lucimarinus	35.19	2.00e-44	171.00
IUUC-Oga-088101	Otolemur garnettii	42.99	6.00e-72	262.00
IUUC-Oar-090293	Ovis aries	42.01	2.00e-69	254.00
IUUC-Ptr-090503	Pan troglodytes	42.99	9.00e-72	261.00
IUUC-Pan-092477	Papio anubis	41.40	5.00e-69	253.00
IUUC-Psi-093442	Pelodiscus sinensis	42.68	4.00e-71	259.00
IUUC-Pma-094247	Petromyzon marinus	40.82	2.00e-60	224.00
IUUC-Pno-094780	Phaeosphaeria nodorum	37.92	2.00e-61	227.00
IUUC-Ppa-095338	Physcomitrella patens	39.09	7.00e-62	229.00
IUUC-Pfo-097137	Poecilia formosa	43.69	1.00e-62	231.00
IUUC-Pab-098060	Pongo abelii	43.31	2.00e-64	237.00
IUUC-Pop-099470	Populus trichocarpa	38.28	5.00e-50	189.00
IUUC-Pca-100500	Procavia capensis	37.74	1.00e-46	178.00
IUUC-Ppe-102008	Prunus persica	34.84	9.00e-40	155.00
IUUC-Pva-103095	Pteropus vampyrus	50.00	2.00e-46	177.00
IUUC-Pgr-103666	Puccinia graminis	43.19	5.00e-65	239.00
IUUC-Ptt-103901	Puccinia triticina	43.85	2.00e-66	244.00
IUUC-Pte-104198	Pyrenophora teres	38.94	7.00e-61	225.00
IUUC-Pyt-104792	Pyrenophora triticirepentis	39.00	3.00e-59	220.00
IUUC-Rno-105446	Rattus norvegicus	42.68	1.00e-71	261.00
IUUC-Sha-106664	Sarcophilus harrisii	42.68	1.00e-70	258.00
IUUC-Sja-107982	Schizosaccharomyces japonicus	42.35	2.00e-67	247.00
IUUC-Spo-108345	Schizosaccharomyces pombe	39.56	4.00e-66	243.00
IUUC-Ssl-108540	Sclerotinia sclerotiorum	38.33	7.00e-50	189.00
IUUC-Smo-109133	Selaginella moellendorffii	38.36	5.00e-60	223.00
IUUC-Sit-109724	Setaria italica	36.24	1.00e-41	162.00
IUUC-Sly-110916	Solanum lycopersicum	38.08	2.00e-50	190.00
IUUC-Stu-112236	Solanum tuberosum	38.08	3.00e-50	190.00
IUUC-Sar-113044	Sorex araneus	42.99	1.00e-71	261.00
IUUC-Sbi-114276	Sorghum bicolor	36.24	2.00e-41	161.00
IUUC-Sre-114963	Sporisorium reilianum	41.47	8.00e-61	225.00
IUUC-Ssc-116301	Sus scrofa	42.01	1.00e-69	254.00
IUUC-Tgu-117265	Taeniopygia guttata	43.31	6.00e-72	262.00
IUUC-Tru-117526	Takifugu rubripes	43.63	6.00e-64	236.00
IUUC-Tsy-119483	Tarsius syrichta	42.36	4.00e-71	259.00
IUUC-Tni-120363	Tetraodon nigroviridis	43.45	1.00e-65	241.00
IUUC-Tca-121731	Theobroma cacao	35.89	2.00e-41	161.00
IUUC-Tre-122221	Trichoderma reesei	38.93	8.00e-50	188.00
IUUC-Tvi-122352	Trichoderma virens	41.67	1.00e-58	218.00
IUUC-Tae-123864	Triticum aestivum	36.59	1.00e-42	165.00
IUUC-Tur-126070	Triticum urartu	34.51	2.00e-32	130.00
IUUC-Tme-127199	Tuber melanosporum	41.22	6.00e-58	216.00
IUUC-Tbe-128076	Tupaia belangeri	42.36	4.00e-71	259.00
IUUC-Ttr-128695	Tursiops truncatus	52.25	1.00e-32	132.00
IUUC-Uma-129638	Ustilago maydis	40.80	2.00e-62	231.00
IUUC-Vda-129963	Verticillium dahliae	41.53	6.00e-53	199.00
IUUC-Vpa-130637	Vicugna pacos	36.57	1.00e-53	201.00
IUUC-Vvi-131613	Vitis vinifera	35.54	5.00e-40	156.00
IUUC-Xtr-132924	Xenopus tropicalis	42.68	5.00e-63	233.00
IUUC-Xma-133356	Xiphophorus maculatus	44.01	1.00e-63	235.00
IUUC-Yli-134365	Yarrowia lipolytica	42.95	8.00e-63	232.00
IUUC-Zma-135941	Zea mays	36.24	2.00e-41	161.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence