iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Loc-052377

Ensembl Protein ID

ENSLOCP00000020254.1

UniProt Accession

W5NHZ5; W5NHZ5_LEPOC

Genbank Protein ID

ENSLOCP00000020254

Protein Name

E3 ubiquitin-protein ligase RNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8

Genbank Nucleotide ID

AHAT01008937

Gene Name

rnf8; RNF8

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSLOCG00000016402.1	ENSLOCT00000020288.1	ENSLOCP00000020254.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/RING/RING	8.40e-07	29.6	395	433

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C+iC e f +  ++l+C H f+++C+  w       ++++ CP+Cr
   Q: 395  CIICSEYFIE-AVTLNCAHSFCLHCIMDW------RRRKDECPICR 433
           ********99.899***************......**********8 PP

Organism

Lepisosteus oculatus

Functional Description
(View)

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains, leading to degradation of substrate proteins. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation.

Protein Sequence
(Fasta)

MAEEAESGAV SGEEDDAAKD EIWCLRRVGK RAEWLRLPEK EEVTLGRSFD VTYQTLSRSC 60
PLMISRNHCK FKQNEEGHWT VTDNKSLNGV WVNGLRITPE RTHTLHVGDT IQLGVPLGGK 120

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Nucleotide Sequence
(Fasta)

ATGGCAGAGG AAGCAGAGTC TGGAGCGGTT TCAGGCGAGG AAGACGATGC CGCGAAAGAT 60
GAAATTTGGT GCCTGAGAAG AGTCGGAAAA AGGGCTGAGT GGCTCCGTCT GCCCGAGAAA 120

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Sequence Source

Ensembl

Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000253--FHA_dom
IPR017335--RNF8
IPR008984--SMAD_FHA_domain
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50006--FHA_DOMAIN
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00498--FHA

SMART

SM00240--FHA
SM00184--RING

Gene Ontology

GO:0008270--F:zinc ion binding

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002124	Ailuropoda melanoleuca	49.68	5.00e-84	305.00
IUUC-Apl-003664	Anas platyrhynchos	73.86	2.00e-33	134.00
IUUC-Aca-005036	Anolis carolinensis	46.83	2.00e-89	322.00
IUUC-Ame-011537	Astyanax mexicanus	53.93	7.00e-120	424.00
IUUC-Bta-013297	Bos taurus	47.68	4.00e-82	298.00
IUUC-Cja-019201	Callithrix jacchus	48.38	5.00e-85	308.00
IUUC-Cfa-021041	Canis familiaris	49.48	5.00e-90	324.00
IUUC-Csa-024133	Chlorocebus sabaeus	48.31	8.00e-89	320.00
IUUC-Cho-024624	Choloepus hoffmanni	40.30	2.00e-42	166.00
IUUC-Cgl-026486	Colletotrichum gloeosporioides	36.99	9.00e-08	52.00
IUUC-Dre-027918	Danio rerio	54.14	4.00e-117	414.00
IUUC-Dno-029774	Dasypus novemcinctus	47.82	8.00e-90	323.00
IUUC-Dor-030190	Dipodomys ordii	39.56	4.00e-59	222.00
IUUC-Ete-032301	Echinops telfairi	43.37	7.00e-60	224.00
IUUC-Eca-033607	Equus caballus	48.14	2.00e-71	263.00
IUUC-Eeu-034888	Erinaceus europaeus	57.92	3.00e-57	215.00
IUUC-Fca-036075	Felis catus	50.32	1.00e-91	330.00
IUUC-Fal-037569	Ficedula albicollis	47.52	2.00e-76	279.00
IUUC-Fso-038129	Fusarium solani	35.62	2.00e-07	50.80
IUUC-Gmo-038588	Gadus morhua	44.44	1.00e-91	330.00
IUUC-Ggr-039871	Gaeumannomyces graminis	36.99	2.00e-07	51.60
IUUC-Gga-040777	Gallus gallus	45.88	2.00e-84	306.00
IUUC-Gac-042463	Gasterosteus aculeatus	50.43	1.00e-85	310.00
IUUC-Ggo-045269	Gorilla gorilla	48.73	7.00e-90	324.00
IUUC-Hsa-046249	Homo sapiens	49.26	3.00e-87	315.00
IUUC-Itr-048314	Ictidomys tridecemlineatus	48.52	2.00e-91	329.00
IUUC-Lch-049805	Latimeria chalumnae	52.73	3.00e-102	365.00
IUUC-Mcc-055344	Macaca mulatta	48.31	1.00e-89	323.00
IUUC-Meu-055950	Macropus eugenii	47.75	3.00e-77	282.00
IUUC-Mor-057012	Magnaporthe oryzae	31.19	2.00e-08	54.70
IUUC-Mga-060049	Meleagris gallopavo	46.47	9.00e-82	297.00
IUUC-Mmr-061431	Microcebus murinus	48.84	2.00e-87	316.00
IUUC-Mdo-062016	Monodelphis domestica	50.45	4.00e-72	267.00
IUUC-Mmu-063235	Mus musculus	45.20	1.00e-89	323.00
IUUC-Mpu-066449	Mustela putorius furo	49.79	4.00e-93	335.00
IUUC-Mlu-067588	Myotis lucifugus	49.15	6.00e-82	298.00
IUUC-Nle-069192	Nomascus leucogenys	48.73	7.00e-90	324.00
IUUC-Opr-070300	Ochotona princeps	39.87	2.00e-69	256.00
IUUC-Ont-071998	Oreochromis niloticus	49.28	2.00e-94	340.00
IUUC-Oan-073610	Ornithorhynchus anatinus	60.12	1.00e-49	189.00
IUUC-Ocu-074603	Oryctolagus cuniculus	47.65	3.00e-85	308.00
IUUC-Ola-086681	Oryzias latipes	46.27	8.00e-93	334.00
IUUC-Oga-088183	Otolemur garnettii	48.55	2.00e-89	322.00
IUUC-Oar-090255	Ovis aries	46.56	3.00e-73	269.00
IUUC-Ptr-091029	Pan troglodytes	48.35	1.00e-81	296.00
IUUC-Pan-092241	Papio anubis	48.31	2.00e-89	322.00
IUUC-Psi-093506	Pelodiscus sinensis	48.58	5.00e-86	311.00
IUUC-Pma-094579	Petromyzon marinus	54.84	3.00e-26	112.00
IUUC-Pfo-097014	Poecilia formosa	51.54	2.00e-92	332.00
IUUC-Pab-098530	Pongo abelii	48.25	4.00e-83	301.00
IUUC-Pca-100939	Procavia capensis	37.61	1.00e-52	200.00
IUUC-Pva-103148	Pteropus vampyrus	44.56	3.00e-75	275.00
IUUC-Rno-105338	Rattus norvegicus	47.69	8.00e-94	337.00
IUUC-Sce-106282	Saccharomyces cerevisiae	33.64	1.00e-07	50.80
IUUC-Sha-107187	Sarcophilus harrisii	46.97	5.00e-60	224.00
IUUC-Sar-113356	Sorex araneus	41.45	4.00e-55	208.00
IUUC-Sre-115193	Sporisorium reilianum	33.33	4.00e-08	53.50
IUUC-Ssc-116290	Sus scrofa	50.00	4.00e-95	341.00
IUUC-Tru-117910	Takifugu rubripes	48.95	9.00e-96	343.00
IUUC-Tsy-119329	Tarsius syrichta	46.78	2.00e-76	279.00
IUUC-Tni-120521	Tetraodon nigroviridis	47.25	2.00e-88	319.00
IUUC-Tre-121992	Trichoderma reesei	31.31	7.00e-08	51.60
IUUC-Ttr-128997	Tursiops truncatus	41.07	1.00e-69	256.00
IUUC-Uma-129518	Ustilago maydis	32.91	2.00e-07	50.80
IUUC-Vpa-130154	Vicugna pacos	45.45	2.00e-80	292.00
IUUC-Xtr-132805	Xenopus tropicalis	46.23	1.00e-88	320.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence