iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Mmu-064260

Ensembl Protein ID

ENSMUSP00000088291.2

UniProt Accession

B2RUR8; Q8CFS0; OTU7B_MOUSE

Genbank Protein ID

EDL38862.1; AAI41398.1; AAH37040.1; AAI41399.1

Protein Name

OTU domain-containing protein 7B; Cellular zinc finger anti-NF-kappa-B protein; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne

Genbank Nucleotide ID

AC092094; BC141397; BC037040; BC141398

Gene Name

Otud7b

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000038495.14	ENSMUST00000090785.8	ENSMUSP00000088291.2

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	miRTarBase	microRNA	TRANSFAC
RepTar	RAID2
Protein-protein Interaction
IID	iRefIndex	HINT
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	UniProt
PHOSIDA	mUbiSiDa
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/OTU	8.20e-37	127.8	184	365
UBD/ZnF/ZnF_A20	6.90e-16	59.0	793	827

Active Site

Position(s)	Description	Evidence
191	N/A	{ECO:0000250}
194	Nucleophile	{ECO:0000269\|PubMed:23334419}
358	Proton acceptor	{ECO:0000269\|PubMed:23334419}

Domain Profile

DUB/OTU

   S: 2    lilktkgDGnCLlrAvsqsllgvfddds.lsdalveaLreltseylqkesdffsefieek...........edekefcqklvepe.... 74
           l+l+t+gDGnCLl+A+s +++g++d+d+ l++al+  + + +++++ ++++++++++++k           ++e++++ kl+++e    
   Q: 184  LPLATTGDGNCLLHAASLGMWGFHDRDLvLRKALYALMEKGVEKEALRRRWRWQQTQQNKesglvytedewQKEWNELIKLASSEprmh 272
           6899********************************************************************************98877 PP
   S: 75   ...akg.................eeihifaLaqiLsrpiivvdvdsles.........kvggillpllfpeek.lkkpivLlYrsgHYd 133
                g                 ee+h+f+La++L+rpi+vv++++l++         ++ggi+lpl++p+++ +++p+vL+Y+++H++
   Q: 273  lgsN-GasgggvesseepvyeslEEFHVFVLAHVLKRPIVVVADTMLRDsggeafapiPFGGIYLPLEVPASQcHRSPLVLAYDQAHFS 360
           7640.256689****************************************************************************** PP
   S: 134  aLval 138
           aLv++
   Q: 361  ALVSM 365
           **986 PP

UBD/ZnF/ZnF_A20

   S: 1    dpskllCkk.gCgfYGnpetngyCSkCyreelrrr 34
           +p++++Ck+ +C+fYG+petn+ CS+Cyreelrrr
   Q: 793  PPTQTKCKQpNCSFYGHPETNNLCSCCYREELRRR 827
           59********************************9 PP

Organism

Mus musculus

Functional Description
(View)

Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B (PubMed:23334419). Negatively regulates mucosal immunity against infections (PubMed:23334419). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (PubMed:26903241). Mediates deubiquitination of EGFR (By similarity). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains. Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure. Hydrolyzes both linear and branched forms of polyubiquitin (By similarity).

Protein Sequence
(Fasta)

MTLDMDAVLS DFVRSTGAEP GLARDLLEGK NWDVSAALSD FEQLRQVHAG NLSPPFSGGS 60
TCPKTPEKGG SDREPTRPSR PILQRQDDVI QEKRLSRGIS HASSSIVSLA RSHVSSNGGG 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CGGACGGCCG AAAGCCACCG ATCCTGAACC AGCCGCTGGA GTCGGAGTGG CGACCCGGTG 60
TGGAGGAGCC GGCGGAAAGC CATTGGGAAG AGGCCAGCTC CGGTCAGTCC CGGCTTGGCT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-1064--Adaptive immunity
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0378--Hydrolase
KW-0391--Immunity
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR003323--OTU_dom
IPR002653--Znf_A20

PROSITE

PS50802--OTU
PS51036--ZF_A20

Pfam

PF02338--OTU
PF01754--zf-A20

SMART

SM00259--ZnF_A20

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0008234--F:cysteine-type peptidase activity
GO:0003677--F:DNA binding
GO:0070530--F:K63-linked polyubiquitin binding
GO:1990380--F:Lys48-specific deubiquitinase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0008270--F:zinc ion binding
GO:0002250--P:adaptive immune response
GO:0002385--P:mucosal immune response
GO:0043124--P:negative regulation of I-kappaB kinase/NF-kappaB signaling
GO:0032717--P:negative regulation of interleukin-8 production
GO:1900181--P:negative regulation of protein localization to nucleus
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0016579--P:protein deubiquitination
GO:0071947--P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0035871--P:protein K11-linked deubiquitination
GO:0071108--P:protein K48-linked deubiquitination
GO:0070536--P:protein K63-linked deubiquitination

KEGG

mmu:229603

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002119	Ailuropoda melanoleuca	90.41	0.00e+00	1383.00
IUUC-Apl-003567	Anas platyrhynchos	65.51	0.00e+00	929.00
IUUC-Bta-013398	Bos taurus	90.88	0.00e+00	1350.00
IUUC-Cel-018468	Caenorhabditis elegans	50.67	6.00e-85	308.00
IUUC-Cja-019965	Callithrix jacchus	89.24	0.00e+00	1327.00
IUUC-Cfa-020191	Canis familiaris	90.17	0.00e+00	1375.00
IUUC-Cpo-022232	Cavia porcellus	88.89	0.00e+00	1311.00
IUUC-Csa-023593	Chlorocebus sabaeus	91.23	0.00e+00	1419.00
IUUC-Cho-024729	Choloepus hoffmanni	81.80	2.00e-171	596.00
IUUC-Csv-026177	Ciona savignyi	30.56	5.00e-03	34.30
IUUC-Dno-029900	Dasypus novemcinctus	89.43	0.00e+00	1337.00
IUUC-Dor-031121	Dipodomys ordii	84.92	0.00e+00	683.00
IUUC-Ete-032473	Echinops telfairi	84.72	0.00e+00	994.00
IUUC-Eca-033946	Equus caballus	91.48	0.00e+00	1012.00
IUUC-Eeu-034670	Erinaceus europaeus	88.51	0.00e+00	1323.00
IUUC-Fca-035871	Felis catus	89.81	0.00e+00	1280.00
IUUC-Fso-038203	Fusarium solani	30.61	2.30e-02	34.70
IUUC-Gmo-039373	Gadus morhua	76.39	0.00e+00	670.00
IUUC-Gga-041105	Gallus gallus	66.32	0.00e+00	971.00
IUUC-Gac-041806	Gasterosteus aculeatus	56.39	0.00e+00	807.00
IUUC-Ggo-045472	Gorilla gorilla	90.76	0.00e+00	1377.00
IUUC-Hsa-046473	Homo sapiens	90.52	0.00e+00	1377.00
IUUC-Itr-048841	Ictidomys tridecemlineatus	91.94	0.00e+00	1400.00
IUUC-Lch-050432	Latimeria chalumnae	62.16	0.00e+00	940.00
IUUC-Mcc-055261	Macaca mulatta	91.00	0.00e+00	1420.00
IUUC-Meu-056477	Macropus eugenii	78.02	0.00e+00	916.00
IUUC-Mga-059533	Meleagris gallopavo	66.24	0.00e+00	963.00
IUUC-Mdo-062847	Monodelphis domestica	80.82	0.00e+00	1139.00
IUUC-Mpu-066876	Mustela putorius furo	85.93	0.00e+00	1272.00
IUUC-Mlu-068039	Myotis lucifugus	89.35	0.00e+00	1340.00
IUUC-Nle-069260	Nomascus leucogenys	90.40	0.00e+00	1377.00
IUUC-Opr-070564	Ochotona princeps	89.12	0.00e+00	763.00
IUUC-Ocu-073824	Oryctolagus cuniculus	92.28	0.00e+00	932.00
IUUC-Oga-088124	Otolemur garnettii	89.82	0.00e+00	1333.00
IUUC-Oar-090086	Ovis aries	91.23	0.00e+00	1354.00
IUUC-Ptr-091622	Pan troglodytes	91.98	0.00e+00	1065.00
IUUC-Pan-092724	Papio anubis	91.00	0.00e+00	1416.00
IUUC-Psi-093333	Pelodiscus sinensis	63.70	0.00e+00	914.00
IUUC-Pab-098667	Pongo abelii	90.40	0.00e+00	1359.00
IUUC-Pca-100701	Procavia capensis	88.86	0.00e+00	712.00
IUUC-Pva-102136	Pteropus vampyrus	89.10	0.00e+00	1325.00
IUUC-Rno-105546	Rattus norvegicus	97.03	0.00e+00	1431.00
IUUC-Sha-106732	Sarcophilus harrisii	81.85	0.00e+00	1053.00
IUUC-Sar-113562	Sorex araneus	78.34	0.00e+00	1068.00
IUUC-Ssc-116191	Sus scrofa	90.76	0.00e+00	1364.00
IUUC-Tgu-116942	Taeniopygia guttata	73.23	0.00e+00	770.00
IUUC-Tsy-119343	Tarsius syrichta	78.91	0.00e+00	1154.00
IUUC-Tbe-127283	Tupaia belangeri	92.37	0.00e+00	740.00
IUUC-Ttr-128692	Tursiops truncatus	87.09	0.00e+00	1261.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence