iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Rno-105449

Ensembl Protein ID

ENSRNOP00000045245.2

UniProt Accession

Q8CIN9; Q6AY29; RFFL_RAT

Genbank Protein ID

AAN60074.1; AAH79216.1

Protein Name

E3 ubiquitin-protein ligase rififylin; FYVE-RING finger protein Sakura; RING finger and FYVE-like domain-containing protein 1; RING-type E3 ubiquitin transferase rififylin

Genbank Nucleotide ID

AY157969; BC079216

Gene Name

Rffl

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSRNOG00000007596.8	ENSRNOT00000041389.4	ENSRNOP00000045245.2
ENSRNOG00000007596.8	ENSRNOT00000076398.2	ENSRNOP00000068077.1
ENSRNOG00000007596.8	ENSRNOT00000076598.2	ENSRNOP00000067950.1

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO
DNA & RNA Element
microRNA	TRANSFAC	RAID2
Protein-protein Interaction
IID
Post-translational Modifications (PTMs)
dbPAF	PhosphositePlus	UniProt
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E3 activity/RING/RING	90.36	0.00e+00	649.00	IUUC-Hsa-045798

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Organism

Rattus norvegicus

Functional Description
(View)

E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Also ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate apoptosis downstream of death domain receptors. Also negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.

Protein Sequence
(Fasta)

MWASCCNWFC LDGQPEETPP PQGARTQAYS NPGYSSFPSP TGSEPSCKAC GVHFASTTRK 60
QTCLDCKKNF CMTCSSQEGN GPRLCLLCLR FRATAFQREE LMKMKVKDLR DYLSLHDIST 120

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Nucleotide Sequence
(Fasta)

GGTGGACTTC CTGGGGCCGC CCGTAAGGAT GTGGTGCTGG CACCCGGGCG CCCCTCAGCC 60
GCGGAGTTGC GGCGGCCGCC GACCCGGCAG CCCCGCTGCC TGAGTGGCCG CAGTGGGGAT 120

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Sequence Source

Ensembl

Keyword

KW-0025--Alternative splicing
KW-0053--Apoptosis
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0967--Endosome
KW-0449--Lipoprotein
KW-0472--Membrane
KW-0479--Metal-binding
KW-0564--Palmitate
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR001370--BIR_rpt
IPR003034--SAP_dom
IPR011011--Znf_FYVE_PHD
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD

PROSITE

PS50089--ZF_RING_2

SMART

SM00184--RING

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0010008--C:endosome membrane
GO:0005886--C:plasma membrane
GO:0055038--C:recycling endosome membrane
GO:0002947--C:tumor necrosis factor receptor superfamily complex
GO:0061630--F:ubiquitin protein ligase activity
GO:0008270--F:zinc ion binding
GO:0006915--P:apoptotic process
GO:2001271--P:negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
GO:1902042--P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
GO:1901797--P:negative regulation of signal transduction by p53 class mediator
GO:0010804--P:negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0070936--P:protein K48-linked ubiquitination
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0010762--P:regulation of fibroblast migration
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

rno:282844

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001630	Ailuropoda melanoleuca	85.40	9.00e-175	605.00
IUUC-Apl-004124	Anas platyrhynchos	65.38	8.00e-123	433.00
IUUC-Ame-011616	Astyanax mexicanus	57.18	2.00e-103	369.00
IUUC-Bta-012374	Bos taurus	85.08	3.00e-178	617.00
IUUC-Cja-019780	Callithrix jacchus	87.64	0.00e+00	630.00
IUUC-Cfa-021252	Canis familiaris	89.26	3.00e-177	613.00
IUUC-Cpo-022047	Cavia porcellus	89.53	0.00e+00	636.00
IUUC-Csa-023161	Chlorocebus sabaeus	89.26	0.00e+00	667.00
IUUC-Cho-024316	Choloepus hoffmanni	86.47	4.00e-144	503.00
IUUC-Dre-028596	Danio rerio	55.52	3.00e-108	384.00
IUUC-Dno-029333	Dasypus novemcinctus	89.53	0.00e+00	632.00
IUUC-Ete-033163	Echinops telfairi	80.11	6.00e-160	556.00
IUUC-Eca-033994	Equus caballus	89.26	0.00e+00	635.00
IUUC-Eeu-035162	Erinaceus europaeus	82.00	5.00e-119	420.00
IUUC-Fca-036418	Felis catus	87.88	0.00e+00	659.00
IUUC-Fal-037427	Ficedula albicollis	65.30	4.00e-130	457.00
IUUC-Gga-041053	Gallus gallus	68.32	5.00e-132	464.00
IUUC-Gac-042281	Gasterosteus aculeatus	44.35	9.00e-74	270.00
IUUC-Ggo-045436	Gorilla gorilla	90.36	0.00e+00	676.00
IUUC-Hsa-045798	Homo sapiens	90.36	0.00e+00	676.00
IUUC-Itr-048077	Ictidomys tridecemlineatus	89.23	2.00e-179	621.00
IUUC-Lch-050639	Latimeria chalumnae	62.26	5.00e-125	440.00
IUUC-Laf-053355	Loxodonta africana	87.16	5.00e-174	603.00
IUUC-Mcc-055222	Macaca mulatta	88.43	0.00e+00	627.00
IUUC-Meu-056692	Macropus eugenii	74.10	3.00e-155	540.00
IUUC-Mga-059298	Meleagris gallopavo	68.04	2.00e-131	461.00
IUUC-Mmr-061792	Microcebus murinus	87.02	2.00e-179	621.00
IUUC-Mdo-062986	Monodelphis domestica	77.96	6.00e-165	573.00
IUUC-Mmu-063356	Mus musculus	97.80	0.00e+00	705.00
IUUC-Mpu-066273	Mustela putorius furo	88.43	0.00e+00	664.00
IUUC-Mlu-066953	Myotis lucifugus	87.88	0.00e+00	645.00
IUUC-Nle-070075	Nomascus leucogenys	89.81	0.00e+00	675.00
IUUC-Opr-070838	Ochotona princeps	85.95	2.00e-174	604.00
IUUC-Ont-071967	Oreochromis niloticus	75.52	2.00e-56	212.00
IUUC-Ocu-074807	Oryctolagus cuniculus	87.05	0.00e+00	645.00
IUUC-Obr-076159	Oryza brachyantha	41.07	4.00e-08	50.40
IUUC-Ola-086903	Oryzias latipes	68.21	7.00e-56	211.00
IUUC-Oga-088536	Otolemur garnettii	87.02	8.00e-176	609.00
IUUC-Oar-089838	Ovis aries	83.70	6.00e-173	599.00
IUUC-Ptr-090962	Pan troglodytes	90.63	0.00e+00	677.00
IUUC-Pan-092853	Papio anubis	88.71	0.00e+00	664.00
IUUC-Psi-093164	Pelodiscus sinensis	69.51	7.00e-124	436.00
IUUC-Pma-094104	Petromyzon marinus	45.02	3.00e-57	215.00
IUUC-Pab-098304	Pongo abelii	89.53	0.00e+00	671.00
IUUC-Pca-100316	Procavia capensis	84.57	1.00e-170	592.00
IUUC-Ppe-101490	Prunus persica	36.59	2.00e-09	57.00
IUUC-Pva-102612	Pteropus vampyrus	72.73	3.00e-148	517.00
IUUC-Sha-107578	Sarcophilus harrisii	79.34	2.00e-151	528.00
IUUC-Sar-113638	Sorex araneus	82.37	6.00e-168	583.00
IUUC-Ssc-116401	Sus scrofa	88.15	0.00e+00	656.00
IUUC-Tru-118765	Takifugu rubripes	50.55	2.00e-83	302.00
IUUC-Tsy-118850	Tarsius syrichta	85.48	2.00e-143	501.00
IUUC-Tbe-128086	Tupaia belangeri	81.54	1.00e-171	595.00
IUUC-Ttr-128249	Tursiops truncatus	67.68	1.00e-133	469.00
IUUC-Vpa-130816	Vicugna pacos	87.21	7.00e-41	160.00
IUUC-Xtr-132262	Xenopus tropicalis	43.16	6.00e-69	254.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence