iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Rno-105927

UUCD1 version

UUC-RaN-00973

Ensembl Protein ID

ENSRNOP00000071283.1

UniProt Accession

Q9EQX9; UBE2N_RAT

Genbank Protein ID

BAB20414.1; AAH90072.1

Protein Name

Ubiquitin-conjugating enzyme E2 N; Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; Ubiquitin carrier protein N; Ubiquitin-protein ligase N

Genbank Nucleotide ID

AB032739; BC090072

Gene Name

Ube2n

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSRNOG00000058053.1	ENSRNOT00000089874.1	ENSRNOP00000071283.1

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO
DNA & RNA Element
microRNA	TRANSFAC	RAID2
Protein-protein Interaction
IID	Mentha
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	UniProt
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E2/UBC	1.30e-47	161.4	7	139

Active Site

Position(s)	Description	Evidence
87	Glycyl thioester intermediate	N/A

Domain Profile

E2/UBC

   S: 1    RlkkelkelekdppegisakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilkee 89
           R+ ke+++l ++p++gi+a+p+++ +   ++v+i Gp+d+p+egg+Fkle+ +pe+YP+ +Pkv+f+tki+hPnv++ G++Cl+ilk  
   Q: 7    RIIKETQRLLAEPVPGIKAEPDES-NARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILK-- 92
           899*********************.9************************************************************9.. PP
   S: 90   ekWspalsvesvllsiqsllaepnpesplneeaaellkknreeykkk 136
           +kWspal++++vllsiq+ll+ pnp++pl +++ae++k+n+++  ++
   Q: 93   DKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQAIET 139
           ****************************************9976655 PP

Organism

Rattus norvegicus

Functional Description
(View)

The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.

Protein Sequence
(Fasta)

MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE 60
EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CTCTGGACGC CCAGTTGCGC GAATTCTGCC GCGGGCGAAG AAGCTCGGCG CCGCCTCGCG 60
CCGCCTCCCC CAAAGTCCCT GCCCCGCCGC GGAGCGTCAC TTCCGCCACC CCGGGCTGGG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0227--DNA damage
KW-0234--DNA repair
KW-1017--Isopeptide bond
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0035370--C:UBC13-UEV1A complex
GO:0000151--C:ubiquitin ligase complex
GO:0005524--F:ATP binding
GO:0061631--F:ubiquitin conjugating enzyme activity
GO:0061630--F:ubiquitin protein ligase activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0006301--P:postreplication repair
GO:0070534--P:protein K63-linked ubiquitination
GO:0016567--P:protein ubiquitination
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

rno:116725

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000100	Aegilops tauschii	79.59	2.00e-69	253.00
IUUC-Aml-001889	Ailuropoda melanoleuca	96.55	1.00e-80	291.00
IUUC-Atr-002818	Amborella trichopoda	79.59	6.00e-70	255.00
IUUC-Apl-003469	Anas platyrhynchos	97.26	7.00e-83	298.00
IUUC-Aca-005051	Anolis carolinensis	99.34	4.00e-87	312.00
IUUC-Aly-006258	Arabidopsis lyrata	80.95	1.00e-70	258.00
IUUC-Ath-007639	Arabidopsis thaliana	80.27	3.00e-70	256.00
IUUC-Ago-007837	Ashbya gossypii	75.20	2.00e-54	204.00
IUUC-Acl-008263	Aspergillus clavatus	71.62	8.00e-64	235.00
IUUC-Afl-008733	Aspergillus flavus	72.30	6.00e-64	236.00
IUUC-Afu-008946	Aspergillus fumigatus	71.62	8.00e-64	235.00
IUUC-Ani-009482	Aspergillus nidulans	71.33	3.00e-64	236.00
IUUC-Ang-009678	Aspergillus niger	72.30	1.00e-63	235.00
IUUC-Aor-010025	Aspergillus oryzae	72.30	6.00e-64	236.00
IUUC-Ate-010254	Aspergillus terreus	71.23	1.00e-62	231.00
IUUC-Ame-010742	Astyanax mexicanus	97.37	3.00e-85	306.00
IUUC-Bgr-012156	Blumeria graminis	65.56	2.00e-54	204.00
IUUC-Bta-012636	Bos taurus	99.34	4.00e-87	312.00
IUUC-Bci-013761	Botrytis cinerea	72.48	4.00e-63	233.00
IUUC-Bdi-014796	Brachypodium distachyon	80.95	1.00e-70	258.00
IUUC-Bol-016613	Brassica oleracea	80.95	1.00e-70	258.00
IUUC-Bra-016938	Brassica rapa	80.95	1.00e-70	258.00
IUUC-Cel-018119	Caenorhabditis elegans	87.92	1.00e-74	271.00
IUUC-Cja-019108	Callithrix jacchus	99.34	4.00e-87	312.00
IUUC-Cfa-020561	Canis familiaris	98.68	2.00e-86	310.00
IUUC-Cpo-022280	Cavia porcellus	96.03	3.00e-83	300.00
IUUC-Cre-022978	Chlamydomonas reinhardtii	78.91	4.00e-67	246.00
IUUC-Csa-023860	Chlorocebus sabaeus	92.11	5.00e-80	289.00
IUUC-Cho-024459	Choloepus hoffmanni	99.34	4.00e-87	312.00
IUUC-Cin-025278	Ciona intestinalis	80.27	7.00e-69	252.00
IUUC-Csv-025952	Ciona savignyi	72.97	2.00e-60	224.00
IUUC-Cgl-026752	Colletotrichum gloeosporioides	71.43	2.00e-61	227.00
IUUC-Cne-026809	Cryptococcus neoformans	74.44	5.00e-57	212.00
IUUC-Cme-027100	Cyanidioschyzon merolae	65.77	1.00e-56	211.00
IUUC-Dre-027931	Danio rerio	98.03	2.00e-85	307.00
IUUC-Dno-030059	Dasypus novemcinctus	98.03	9.00e-86	308.00
IUUC-Dor-030269	Dipodomys ordii	99.22	5.00e-73	265.00
IUUC-Dse-031505	Dothistroma septosporum	68.03	9.00e-60	221.00
IUUC-Dme-032097	Drosophila melanogaster	80.13	2.00e-72	263.00
IUUC-Ete-032369	Echinops telfairi	99.34	4.00e-87	312.00
IUUC-Eca-033230	Equus caballus	99.34	4.00e-87	312.00
IUUC-Fca-036161	Felis catus	94.74	6.00e-83	298.00
IUUC-Fal-036781	Ficedula albicollis	96.05	2.00e-84	303.00
IUUC-Fox-037840	Fusarium oxysporum	71.92	8.00e-61	225.00
IUUC-Fso-038118	Fusarium solani	71.92	5.00e-61	226.00
IUUC-Gmo-039561	Gadus morhua	92.76	2.00e-82	297.00
IUUC-Ggr-039807	Gaeumannomyces graminis	72.79	1.00e-62	231.00
IUUC-Gga-041024	Gallus gallus	99.34	4.00e-87	312.00
IUUC-Gac-042450	Gasterosteus aculeatus	92.72	3.00e-81	293.00
IUUC-Gma-043982	Glycine max	80.95	1.00e-70	258.00
IUUC-Ggo-044660	Gorilla gorilla	98.68	2.00e-86	310.00
IUUC-Hsa-045881	Homo sapiens	99.34	4.00e-87	312.00
IUUC-Hvu-047346	Hordeum vulgare	80.95	1.00e-70	258.00
IUUC-Itr-048350	Ictidomys tridecemlineatus	91.45	3.00e-78	283.00
IUUC-Kpa-049285	Komagataella pastoris	70.27	6.00e-63	232.00
IUUC-Lch-050548	Latimeria chalumnae	94.41	3.00e-79	286.00
IUUC-Lpe-051394	Leersia perrieri	80.27	2.00e-70	257.00
IUUC-Loc-052205	Lepisosteus oculatus	97.37	3.00e-85	307.00
IUUC-Lma-053056	Leptosphaeria maculans	72.60	1.00e-62	231.00
IUUC-Laf-053540	Loxodonta africana	99.30	3.00e-81	293.00
IUUC-Mcc-054863	Macaca mulatta	99.34	4.00e-87	312.00
IUUC-Meu-056482	Macropus eugenii	99.30	3.00e-81	293.00
IUUC-Mor-057014	Magnaporthe oryzae	72.60	3.00e-61	226.00
IUUC-Mpo-057586	Magnaporthe poae	72.79	1.00e-62	231.00
IUUC-Mtr-058552	Medicago truncatula	72.67	8.00e-67	245.00
IUUC-Mla-058920	Melampsora laricipopulina	69.39	1.00e-60	225.00
IUUC-Mga-059649	Meleagris gallopavo	95.97	4.00e-82	296.00
IUUC-Mvi-060483	Microbotryum violaceum	75.51	4.00e-65	239.00
IUUC-Mmr-061167	Microcebus murinus	99.34	4.00e-87	312.00
IUUC-Mdo-062701	Monodelphis domestica	99.34	4.00e-87	312.00
IUUC-Mmu-064070	Mus musculus	99.34	4.00e-87	312.00
IUUC-Mac-064742	Musa acuminata	80.27	1.00e-70	258.00
IUUC-Mpu-066401	Mustela putorius furo	99.34	5.00e-87	312.00
IUUC-Mlu-067229	Myotis lucifugus	97.96	1.00e-82	297.00
IUUC-Nfi-068526	Neosartorya fischeri	71.62	9.00e-64	235.00
IUUC-Ncr-068643	Neurospora crassa	72.11	3.00e-62	230.00
IUUC-Nle-070146	Nomascus leucogenys	99.34	4.00e-87	312.00
IUUC-Opr-070232	Ochotona princeps	77.63	4.00e-63	233.00
IUUC-Ont-072369	Oreochromis niloticus	94.00	4.00e-82	296.00
IUUC-Oan-073174	Ornithorhynchus anatinus	95.39	3.00e-83	300.00
IUUC-Ocu-074712	Oryctolagus cuniculus	99.34	4.00e-87	312.00
IUUC-Oba-075408	Oryza barthii	80.27	2.00e-70	257.00
IUUC-Obr-076881	Oryza brachyantha	80.27	2.00e-70	257.00
IUUC-Ogl-077242	Oryza glaberrima	80.27	2.00e-70	257.00
IUUC-Ogu-078465	Oryza glumaepatula	80.27	2.00e-70	257.00
IUUC-Oin-080035	Oryza indica	80.43	4.00e-66	243.00
IUUC-Olo-080555	Oryza longistaminata	80.27	2.00e-70	257.00
IUUC-Ome-081653	Oryza meridionalis	80.27	2.00e-70	257.00
IUUC-Oni-082400	Oryza nivara	80.27	2.00e-70	257.00
IUUC-Opu-083130	Oryza punctata	80.43	2.00e-65	241.00
IUUC-Oru-084649	Oryza rufipogon	80.27	2.00e-70	257.00
IUUC-Osa-085629	Oryza sativa	80.27	2.00e-70	257.00
IUUC-Ola-087083	Oryzias latipes	95.33	2.00e-82	297.00
IUUC-Olu-087886	Ostreococcus lucimarinus	67.35	3.00e-58	216.00
IUUC-Oga-088983	Otolemur garnettii	95.33	1.00e-82	298.00
IUUC-Oar-090440	Ovis aries	98.68	2.00e-86	310.00
IUUC-Ptr-090572	Pan troglodytes	99.34	4.00e-87	312.00
IUUC-Pan-092503	Papio anubis	97.37	2.00e-85	307.00
IUUC-Psi-093419	Pelodiscus sinensis	99.26	9.00e-78	281.00
IUUC-Pma-094136	Petromyzon marinus	92.86	3.00e-43	165.00
IUUC-Pno-094838	Phaeosphaeria nodorum	72.30	2.00e-63	234.00
IUUC-Ppa-095892	Physcomitrella patens	81.63	2.00e-70	257.00
IUUC-Pfo-096074	Poecilia formosa	96.00	5.00e-83	299.00
IUUC-Pab-097778	Pongo abelii	99.34	4.00e-87	312.00
IUUC-Pop-099707	Populus trichocarpa	79.59	7.00e-70	255.00
IUUC-Pca-100334	Procavia capensis	82.89	1.00e-72	264.00
IUUC-Ppe-101149	Prunus persica	80.95	1.00e-70	258.00
IUUC-Pva-102979	Pteropus vampyrus	99.34	4.00e-87	312.00
IUUC-Pgr-103654	Puccinia graminis	71.43	8.00e-61	225.00
IUUC-Ptt-103752	Puccinia triticina	70.14	2.00e-58	217.00
IUUC-Pte-104137	Pyrenophora teres	71.23	9.00e-62	228.00
IUUC-Pyt-104513	Pyrenophora triticirepentis	71.23	9.00e-62	228.00
IUUC-Sce-106178	Saccharomyces cerevisiae	70.27	1.00e-61	228.00
IUUC-Sha-107493	Sarcophilus harrisii	99.34	4.00e-87	312.00
IUUC-Sja-107878	Schizosaccharomyces japonicus	66.89	8.00e-58	216.00
IUUC-Spo-108062	Schizosaccharomyces pombe	68.71	6.00e-57	212.00
IUUC-Ssl-108395	Sclerotinia sclerotiorum	70.95	2.00e-61	227.00
IUUC-Smo-109587	Selaginella moellendorffii	74.00	2.00e-63	234.00
IUUC-Sit-110151	Setaria italica	80.95	2.00e-70	258.00
IUUC-Sly-111322	Solanum lycopersicum	80.95	1.00e-70	258.00
IUUC-Stu-112722	Solanum tuberosum	80.27	4.00e-70	256.00
IUUC-Sar-113464	Sorex araneus	99.30	3.00e-81	293.00
IUUC-Sbi-114864	Sorghum bicolor	80.27	7.00e-70	255.00
IUUC-Sre-114927	Sporisorium reilianum	78.08	7.00e-67	245.00
IUUC-Ssc-116096	Sus scrofa	99.34	4.00e-87	312.00
IUUC-Tgu-117266	Taeniopygia guttata	99.30	3.00e-81	293.00
IUUC-Tru-117903	Takifugu rubripes	94.04	8.00e-82	295.00
IUUC-Tsy-118952	Tarsius syrichta	99.30	3.00e-81	293.00
IUUC-Tni-120038	Tetraodon nigroviridis	94.04	8.00e-82	295.00
IUUC-Tca-121752	Theobroma cacao	79.86	2.00e-65	241.00
IUUC-Tre-121979	Trichoderma reesei	73.97	3.00e-62	230.00
IUUC-Tvi-122515	Trichoderma virens	73.29	2.00e-61	228.00
IUUC-Tae-125309	Triticum aestivum	80.95	1.00e-70	258.00
IUUC-Tur-126687	Triticum urartu	78.72	3.00e-65	240.00
IUUC-Tme-127129	Tuber melanosporum	73.97	4.00e-63	233.00
IUUC-Tbe-127846	Tupaia belangeri	99.30	3.00e-81	293.00
IUUC-Ttr-128808	Tursiops truncatus	97.37	3.00e-84	303.00
IUUC-Uma-129548	Ustilago maydis	78.77	2.00e-67	247.00
IUUC-Vda-129810	Verticillium dahliae	71.23	8.00e-61	225.00
IUUC-Vpa-130221	Vicugna pacos	90.65	7.00e-69	251.00
IUUC-Vvi-131416	Vitis vinifera	66.85	7.00e-66	242.00
IUUC-Xtr-132366	Xenopus tropicalis	96.10	3.00e-84	303.00
IUUC-Xma-132986	Xiphophorus maculatus	95.33	1.00e-82	297.00
IUUC-Yli-134605	Yarrowia lipolytica	72.97	1.00e-63	235.00
IUUC-Zma-135587	Zea mays	77.12	3.00e-68	250.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence