iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
DNA & RNA Element
microRNA	TRANSFAC	RAID2
Protein-protein Interaction
IID
Post-translational Modifications (PTMs)
dbPAF	PhosphositePlus	UniProt
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/USP	1.10e-67	229.1	412	790

Active Site

Position(s)	Description	Evidence
420	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}
745	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 2    GlkNlgNtCYmNSvLQcLsnipelrelllekkkeklneenpkepkgellkslqklfeelw......kskkk.............avspr 71
           Gl N gN CY+N++LQ+L+++p++ +l++   +   +  +++ +++++++s+ +l++e++      k ++              a++p+
   Q: 412  GLINKGNWCYINATLQALVACPPMYHLMKF-IPLYSK-VQRPCTSTPMIDSFVRLMNEFTnmpvppKPRQAlgdkivrdirpgaAFEPT 498
           9***************************99.333333.334799***************7666654333337777888888888***** PP
   S: 72   ellkalkkeaeefs.gyeqqDaqEflaflldqleeelkk.................eknkpkeeeeeedkselsde............. 129
            +++ l  +++++s + +q+Da+E+l f+l++l+ee+ +                                    +             
   Q: 499  YIYRLLTVIKSSLSeKGRQEDAEEYLGFILNGLHEEMLSlkkllspthekhsvsngPG-----------------Shliedeeledtge 570
           *9999999999966599********************545545555544444444422.................02344444555566 PP
   S: 130  ............aeekkkkknk...........slisdlFqgqlksqikclecdnvssreepfldlslpvpgkeetkkeslqdcLesft 195
                       +        k           ++i+ +F+g+++ +++ ++  ++s + +pf++l+l++++++ +   ++qd+Les +
   Q: 571  gsedeweqvgpkN--------KtsvtrqadfvqTPITGIFGGHIR-SVVYQQSSKESATLQPFFTLQLDIQSDKIR---TVQDALESLV 647
           6666555555551........333444555666***********9.688999999*******************99...********** PP
   S: 196  keeelegenkyycekckkkqeaekklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfctspeekqke...kkkYeLi 281
           ++e+++g    y++k k+++e++++++l+klP+vL+lhLkRf ++++++++ k+ k++e+p  L++s+ + sp  k+k+   +++Y+L+
   Q: 648  ARESVQG----YTTKTKQEVEVSRRVTLEKLPPVLVLHLKRFVYEKTGGCQ-KLVKNIEYPVDLEISRELLSPGVKNKNfkcHRTYRLF 731
           ******8....99999****************************9998886.***************9999987777767779****** PP
   S: 282  avvvHsG.slssGHYvayvknekgk.WllfdDskVtevkeeevleetg.staYvlfYkr 337
           avv+H+G s+ +GHY+++v +   + Wl++dD++V+++++ +v++ ++ +taY+l+Y+r
   Q: 732  AVVYHHGnSATGGHYTTDVFQIGLNgWLRIDDQTVKVINQYQVVRPSAdRTAYLLYYRR 790
           *********************87777*****************998778********98 PP

Organism

Rattus norvegicus

Functional Description
(View)

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage.

Protein Sequence
(Fasta)

MALHNPQYIF GDFSPDEFNQ FFVTPRSSVE LPPYSGTQCG IQAEEELLDG QEHQRIEFGV 60
DEVIEPSDGL QRAPSYSISS TLNPQAPEFI LGCPTSKKTP DDIEKDETYS SIDQYPASAL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GTGTCTGTGC GGCGAGAAGA TGGCGGCGAC GGGGGACGCG GCGTGAAAAG CCGGAGGAGC 60
TCCGAACCTC ATTGAGACGG GCCGCCATGG CCCTCCACAA CCCACAGGTA GCGCGGGCCG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0227--DNA damage
KW-0234--DNA repair
KW-0967--Endosome
KW-0378--Hydrolase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR009818--Ataxin-2_C
IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF07145--PAM2
PF00443--UCH

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005769--C:early endosome
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0043234--C:protein complex
GO:0004197--F:cysteine-type endopeptidase activity
GO:0044325--F:ion channel binding
GO:0002039--F:p53 binding
GO:0003723--F:RNA binding
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0006914--P:autophagy
GO:0006974--P:cellular response to DNA damage stimulus
GO:0071347--P:cellular response to interleukin-1
GO:0030330--P:DNA damage response, signal transduction by p53 class mediator
GO:0006281--P:DNA repair
GO:0043124--P:negative regulation of I-kappaB kinase/NF-kappaB signaling
GO:0016579--P:protein deubiquitination
GO:0010506--P:regulation of autophagy
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

rno:307905

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000082	Aegilops tauschii	31.31	3.00e-46	180.00
IUUC-Aml-001584	Ailuropoda melanoleuca	81.11	0.00e+00	1199.00
IUUC-Atr-002927	Amborella trichopoda	36.25	1.00e-60	227.00
IUUC-Apl-003988	Anas platyrhynchos	75.00	0.00e+00	1093.00
IUUC-Aca-004461	Anolis carolinensis	86.53	3.00e-161	560.00
IUUC-Aly-006038	Arabidopsis lyrata	35.97	9.00e-56	211.00
IUUC-Ath-007328	Arabidopsis thaliana	36.22	3.00e-54	206.00
IUUC-Ago-007717	Ashbya gossypii	31.24	1.00e-47	184.00
IUUC-Acl-008188	Aspergillus clavatus	35.99	9.00e-64	238.00
IUUC-Afl-008456	Aspergillus flavus	38.49	2.00e-49	189.00
IUUC-Afu-009067	Aspergillus fumigatus	37.47	3.00e-67	250.00
IUUC-Ani-009483	Aspergillus nidulans	33.54	4.00e-62	233.00
IUUC-Ang-009639	Aspergillus niger	35.60	9.00e-61	228.00
IUUC-Aor-010040	Aspergillus oryzae	35.98	1.00e-61	231.00
IUUC-Ate-010310	Aspergillus terreus	35.18	2.00e-62	234.00
IUUC-Ame-011012	Astyanax mexicanus	74.94	7.00e-167	581.00
IUUC-Bgr-012311	Blumeria graminis	34.95	4.00e-45	176.00
IUUC-Bta-013299	Bos taurus	80.52	0.00e+00	1167.00
IUUC-Bci-013783	Botrytis cinerea	37.63	3.00e-59	223.00
IUUC-Bdi-015039	Brachypodium distachyon	32.76	1.00e-50	194.00
IUUC-Bol-015806	Brassica oleracea	36.99	3.00e-63	236.00
IUUC-Bra-017076	Brassica rapa	37.24	2.00e-63	237.00
IUUC-Cja-018715	Callithrix jacchus	82.62	0.00e+00	1251.00
IUUC-Cfa-020194	Canis familiaris	82.12	0.00e+00	1221.00
IUUC-Cpo-022272	Cavia porcellus	74.15	0.00e+00	1027.00
IUUC-Cre-022713	Chlamydomonas reinhardtii	35.88	4.00e-36	146.00
IUUC-Csa-023310	Chlorocebus sabaeus	83.50	0.00e+00	1261.00
IUUC-Cho-024966	Choloepus hoffmanni	72.42	0.00e+00	858.00
IUUC-Cin-025616	Ciona intestinalis	47.13	5.00e-106	378.00
IUUC-Csv-026043	Ciona savignyi	46.34	4.00e-100	358.00
IUUC-Cgl-026421	Colletotrichum gloeosporioides	34.13	2.00e-46	180.00
IUUC-Cne-026962	Cryptococcus neoformans	35.79	9.00e-51	195.00
IUUC-Cme-027113	Cyanidioschyzon merolae	30.68	7.00e-40	158.00
IUUC-Dre-028072	Danio rerio	76.99	0.00e+00	723.00
IUUC-Dno-029489	Dasypus novemcinctus	81.86	0.00e+00	1256.00
IUUC-Dor-031068	Dipodomys ordii	74.47	5.00e-143	501.00
IUUC-Dse-031553	Dothistroma septosporum	37.11	3.00e-59	223.00
IUUC-Dme-031759	Drosophila melanogaster	41.72	4.00e-84	306.00
IUUC-Ete-032549	Echinops telfairi	73.55	0.00e+00	809.00
IUUC-Eca-033380	Equus caballus	81.74	0.00e+00	1239.00
IUUC-Eeu-034818	Erinaceus europaeus	79.62	0.00e+00	1179.00
IUUC-Fca-035535	Felis catus	79.92	0.00e+00	1098.00
IUUC-Fal-036627	Ficedula albicollis	73.21	0.00e+00	1061.00
IUUC-Fox-037904	Fusarium oxysporum	37.06	3.00e-56	213.00
IUUC-Fso-038170	Fusarium solani	33.79	2.00e-53	204.00
IUUC-Gmo-039499	Gadus morhua	67.68	0.00e+00	668.00
IUUC-Ggr-040042	Gaeumannomyces graminis	37.27	2.00e-63	238.00
IUUC-Gga-040571	Gallus gallus	75.28	0.00e+00	1091.00
IUUC-Gac-041345	Gasterosteus aculeatus	74.57	0.00e+00	656.00
IUUC-Gma-044407	Glycine max	36.15	3.00e-60	226.00
IUUC-Ggo-044652	Gorilla gorilla	84.00	0.00e+00	1299.00
IUUC-Hsa-045864	Homo sapiens	84.12	0.00e+00	1301.00
IUUC-Hvu-047071	Hordeum vulgare	33.50	4.00e-54	205.00
IUUC-Itr-048216	Ictidomys tridecemlineatus	88.98	0.00e+00	1019.00
IUUC-Kpa-049132	Komagataella pastoris	31.70	2.00e-48	187.00
IUUC-Lch-050040	Latimeria chalumnae	68.20	0.00e+00	895.00
IUUC-Loc-052449	Lepisosteus oculatus	77.99	0.00e+00	709.00
IUUC-Lma-053248	Leptosphaeria maculans	36.00	1.00e-58	221.00
IUUC-Laf-054121	Loxodonta africana	81.99	0.00e+00	1226.00
IUUC-Mcc-055514	Macaca mulatta	83.50	0.00e+00	1259.00
IUUC-Meu-056873	Macropus eugenii	69.59	0.00e+00	787.00
IUUC-Mor-057243	Magnaporthe oryzae	33.67	3.00e-58	220.00
IUUC-Mpo-057439	Magnaporthe poae	36.68	3.00e-62	233.00
IUUC-Mtr-058457	Medicago truncatula	34.87	3.00e-59	222.00
IUUC-Mla-058929	Melampsora laricipopulina	31.90	4.00e-59	223.00
IUUC-Mga-060157	Meleagris gallopavo	74.35	0.00e+00	1087.00
IUUC-Mvi-060442	Microbotryum violaceum	33.08	2.00e-55	211.00
IUUC-Mmr-061062	Microcebus murinus	83.98	0.00e+00	1291.00
IUUC-Mdo-062798	Monodelphis domestica	77.36	0.00e+00	1160.00
IUUC-Mmu-064050	Mus musculus	92.70	0.00e+00	1453.00
IUUC-Mac-065619	Musa acuminata	35.04	1.00e-55	210.00
IUUC-Mpu-066554	Mustela putorius furo	81.76	0.00e+00	1195.00
IUUC-Mlu-068003	Myotis lucifugus	82.00	0.00e+00	1234.00
IUUC-Nfi-068612	Neosartorya fischeri	37.20	1.00e-64	241.00
IUUC-Ncr-068878	Neurospora crassa	35.40	2.00e-53	204.00
IUUC-Nle-069661	Nomascus leucogenys	84.25	0.00e+00	1282.00
IUUC-Opr-070944	Ochotona princeps	79.36	9.00e-162	563.00
IUUC-Ont-071266	Oreochromis niloticus	77.19	0.00e+00	688.00
IUUC-Oan-073442	Ornithorhynchus anatinus	76.76	0.00e+00	1071.00
IUUC-Ocu-073788	Oryctolagus cuniculus	76.11	0.00e+00	1065.00
IUUC-Oba-075085	Oryza barthii	34.53	3.00e-38	151.00
IUUC-Obr-076663	Oryza brachyantha	33.93	5.00e-47	182.00
IUUC-Ogl-076937	Oryza glaberrima	30.48	9.00e-50	191.00
IUUC-Ogu-078909	Oryza glumaepatula	30.42	2.00e-47	184.00
IUUC-Oin-079617	Oryza indica	32.00	1.00e-49	191.00
IUUC-Olo-081043	Oryza longistaminata	26.59	1.00e-25	110.00
IUUC-Ome-081646	Oryza meridionalis	25.26	4.00e-24	106.00
IUUC-Oni-082914	Oryza nivara	30.42	1.00e-47	184.00
IUUC-Oru-084878	Oryza rufipogon	30.42	1.00e-47	184.00
IUUC-Osa-085822	Oryza sativa	31.29	5.00e-52	198.00
IUUC-Ola-086931	Oryzias latipes	57.94	0.00e+00	777.00
IUUC-Oga-088443	Otolemur garnettii	83.75	0.00e+00	1286.00
IUUC-Oar-089325	Ovis aries	79.39	0.00e+00	1098.00
IUUC-Ptr-091584	Pan troglodytes	81.00	0.00e+00	1208.00
IUUC-Pan-092551	Papio anubis	81.75	0.00e+00	1234.00
IUUC-Psi-093366	Pelodiscus sinensis	70.70	0.00e+00	653.00
IUUC-Pno-095136	Phaeosphaeria nodorum	35.77	8.00e-54	205.00
IUUC-Ppa-095574	Physcomitrella patens	37.78	5.00e-61	228.00
IUUC-Pfo-097462	Poecilia formosa	77.25	0.00e+00	691.00
IUUC-Pab-098547	Pongo abelii	84.12	0.00e+00	1275.00
IUUC-Pop-099529	Populus trichocarpa	37.44	2.00e-63	236.00
IUUC-Pca-101066	Procavia capensis	73.84	0.00e+00	1088.00
IUUC-Ppe-102110	Prunus persica	36.90	2.00e-61	229.00
IUUC-Pva-103015	Pteropus vampyrus	76.65	0.00e+00	1141.00
IUUC-Ptt-103998	Puccinia triticina	33.00	2.00e-55	210.00
IUUC-Pte-104417	Pyrenophora teres	33.42	1.00e-54	208.00
IUUC-Pyt-104820	Pyrenophora triticirepentis	34.68	2.00e-54	207.00
IUUC-Sce-106160	Saccharomyces cerevisiae	27.85	3.00e-38	153.00
IUUC-Sha-106590	Sarcophilus harrisii	77.23	0.00e+00	1154.00
IUUC-Sja-107669	Schizosaccharomyces japonicus	34.93	3.00e-57	216.00
IUUC-Spo-108353	Schizosaccharomyces pombe	37.56	2.00e-65	243.00
IUUC-Ssl-108599	Sclerotinia sclerotiorum	36.39	3.00e-57	216.00
IUUC-Smo-108842	Selaginella moellendorffii	35.84	1.00e-38	153.00
IUUC-Sit-110781	Setaria italica	32.65	4.00e-57	216.00
IUUC-Sly-111797	Solanum lycopersicum	34.53	6.00e-57	215.00
IUUC-Stu-112188	Solanum tuberosum	35.61	2.00e-56	213.00
IUUC-Sbi-114862	Sorghum bicolor	33.61	6.00e-47	182.00
IUUC-Sre-115162	Sporisorium reilianum	35.70	7.00e-68	252.00
IUUC-Ssc-115505	Sus scrofa	93.20	1.00e-164	571.00
IUUC-Tgu-116535	Taeniopygia guttata	73.05	0.00e+00	1069.00
IUUC-Tru-117542	Takifugu rubripes	76.13	0.00e+00	718.00
IUUC-Tni-120200	Tetraodon nigroviridis	57.14	0.00e+00	815.00
IUUC-Tca-121548	Theobroma cacao	36.19	2.00e-45	177.00
IUUC-Tre-122124	Trichoderma reesei	34.60	3.00e-60	226.00
IUUC-Tvi-122457	Trichoderma virens	34.88	2.00e-54	207.00
IUUC-Tae-125646	Triticum aestivum	34.68	1.00e-56	214.00
IUUC-Tme-127180	Tuber melanosporum	40.43	5.00e-72	265.00
IUUC-Tbe-127530	Tupaia belangeri	69.59	0.00e+00	963.00
IUUC-Ttr-128549	Tursiops truncatus	74.09	0.00e+00	1080.00
IUUC-Uma-129381	Ustilago maydis	34.12	1.00e-66	248.00
IUUC-Vda-129889	Verticillium dahliae	32.89	3.00e-52	200.00
IUUC-Vpa-130100	Vicugna pacos	76.60	0.00e+00	1100.00
IUUC-Vvi-131366	Vitis vinifera	36.64	3.00e-65	243.00
IUUC-Xtr-132048	Xenopus tropicalis	62.30	0.00e+00	914.00
IUUC-Xma-133593	Xiphophorus maculatus	77.25	0.00e+00	687.00
IUUC-Yli-134338	Yarrowia lipolytica	31.42	2.00e-34	140.00
IUUC-Zma-135586	Zea mays	32.12	5.00e-50	192.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence