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Basic Information Integrated Annotations

Tag Content
UUCD2 ID IUUC-Sce-106425
UUCD1 version UUC-SaC-00080
Ensembl Protein ID YBR195C
UniProt Accession P13712; D6VQI9; MSI1_YEAST
Genbank Protein ID AAA34804.1; CAA79682.1; CAA85157.1; AAT92853.1; DAA07309.1
Protein Name Chromatin assembly factor 1 subunit p50; IRA1 multicopy suppressor; Protein MSI1
Genbank Nucleotide ID M27300; Z21487; Z36064; AY692834; BK006936
Gene Name MSI1; CAC3; YBR195C; YBR1405
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
YBR195C YBR195C YBR195C
Annotation
mRNA Expression
GEOFFGED
Protein-protein Interaction
IIDiRefIndexHINTMentha
Post-translational Modifications (PTMs)
CPLMdbPAFdbPTMBioGRID
Protein Expression/Proteomics
GPMDB
Status Unreviewed
Classification
Family E-Value Score Start End
E3 adaptor/Cullin RING/DCX/DWD 2.30e-24 82.9 382 413
Active Site
Position(s) Description Evidence
N/A N/A N/A
Domain Profile

   E3 adaptor/Cullin RING/DCX/DWD

   S: 1    gHtdsvtclaf..sd.tllvsGsdDgtikvWD 29
    gH +v+++++ +d +l++s ++D+++++W
   Q: 382 GHMLGVNDISWdaHDpWLMCSVANDNSVHIWK 413
    68889********888***************8 PP
   

Organism Saccharomyces cerevisiae
Functional Description
(View)

Functional Description



     Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts (By similarity). Independently, MSI1 is involved in regulation of the RAS/cAMP pathway via sequestration of NPR1.
Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts (By similarity). Independently, MSI1 is involved in regulation of the RAS/cAMP pathway via sequestration of NPR1.
Protein Sequence
(Fasta)
MNQCAKDITH EASSIPIDLQ ERYSHWKKNT KLLYDYLNTN STKWPSLTCQ FFPDLDTTSD 60
EHRILLSSFT SSQKPEDETI YISKISTLGH IKWSSLNNFD MDEMEFKPEN STRFPSKHLV 120
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Protein Fasta Sequence



>IUUC-Sce-106425|E3,WD_repeat|Saccharomyces cerevisiae
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Nucleotide Sequence
(Fasta)
ATGAATCAGT GCGCGAAGGA CATAACTCAT GAAGCCTCCA GTATACCCAT CGATTTGCAA 60
GAAAGATACT CGCACTGGAA GAAAAACACT AAACTACTTT ATGATTACCT AAACACGAAT 120
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Nucleotide Fasta Sequence



>IUUC-Sce-106425|E3,WD_repeat|Saccharomyces cerevisiae
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Sequence Source Ensembl
Keyword

KW-0114--cAMP
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0853--WD repeat

Interpro

IPR022052--Histone-bd_RBBP4_N
IPR013979--TIF_beta_prop-like
IPR015943--WD40/YVTN_repeat-like_dom
IPR001680--WD40_repeat
IPR019775--WD40_repeat_CS
IPR017986--WD40_repeat_dom

PROSITE

PS00678--WD_REPEATS_1
PS50082--WD_REPEATS_2
PS50294--WD_REPEATS_REGION

Pfam

PF12265--CAF1C_H4-bd
PF08662--eIF2A
PF00400--WD40

SMART

SM00320--WD40

Gene Ontology

GO:0033186--C:CAF-1 complex
GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0006335--P:DNA replication-dependent nucleosome assembly

KEGG sce:YBR195C
Orthology
iUUCD ID Species Identity E-value Score
IUUC-Ago-007712 Ashbya gossypii 55.71 2.00e-138 483.00
IUUC-Kpa-049119 Komagataella pastoris 34.93 4.00e-60 223.00
Created Date 25-Jun-2017

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