iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Family	Domain	References (PMIDs)
E3 adaptor/Cullin RING/SCF/F-box	F-box	18256526; 17344412; 9990507; 14970237
E3 adaptor/Cullin RING/DCX/DWD	WD_repeat	18256526; 17344412; 9990507; 14970237

Classification

Family	E-value	Score	Start	End
E3 adaptor/Cullin RING/DCX/DWD	1.00e-45	149.8	445	480
E3 adaptor/Cullin RING/SCF/F-box	0.0015	16.7	300	345
UBD/Beta-Prp	2.50e-61	205.5	408	698

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 adaptor/Cullin RING/DCX/DWD

   S: 2    Htdsvtclaf.sdtllvsGsdDgtikvWDlrtgvllk 37
           H+++v+ +++   ++lv+Gs D t++vWDlrtg + +
   Q: 445  HEGDVWTFEYvG-DTLVTGSTDRTVRVWDLRTGECKQ 480
           999*****9964.679******************987 PP

E3 adaptor/Cullin RING/SCF/F-box

   S: 102  lldrlpeeilveilklldkeellraarvcrrwq.elasqkalwktv 146
            l+ +p ei   +l++ld+++l+++++v+++w+  ++s+++lwk++
   Q: 300  FLTGFPAEITNLVLTHLDAPSLCAVSQVSHHWYkLVSSNEELWKSL 345
           567789***************************6677799***986 PP

UBD/Beta-Prp

   S: 12   vitcvkfspdksllatgsrdgtikvwdvttgkllrslsgheaevtavkfspdgkilvtgseDrtikvwdlstgkcvhtlkghedsvls.... 99
            it+++f +d++++++++ + +i ++d++tg l+rsl++he+ v++ ++  d  +lvtgs+Drt++vwdl+tg+c +++ gh++++++    
   Q: 408  LITKLQF-DDDKIIVSTC-SPRINIYDTKTGVLIRSLEEHEGDVWTFEYVGD--TLVTGSTDRTVRVWDLRTGECKQVFYGHTSTIRCikiv 495
           699****.7788888888.**************************9999999..8************************************* PP
   S: 100  ......................lsfspsgdklvsGshdrtirvwdletgqclee...........................tlsghdgvvnc 142
                                  +++p   +++s s+d tir+w   +  cl +                           tl+gh+++v+ 
   Q: 496  qgnqsttdtddvekenrpasndANSMP--PYIISSSRDCTIRLW---SLPCLDDppfvnvnenpdqnndftsattnpfyirTLRGHTDSVRE 582
           *****************9775555555..***************...6668888899999******************************** PP
   S: 143  vvvhpdgnllvsGslDrtvrlWdlktgkllrtltgghtdsvyslsfdsngkelvsgsldgtvklwdlqtgecvrtlnghtdnvksvrfspnd 234
           v+  +  +l+vs+s+D+t+r+W+ +tg +l+ l+g h ++vys+++++++++++s+++d ++++w+l++ge ++tl+gh++ v++v f   +
   Q: 583  VACLG--DLIVSASYDGTLRVWKASTGVCLHVLRG-HVGRVYSVTINPSRQQCISAGTDAKIRIWNLESGELLQTLHGHSNLVSQVTFN--Q 669
           *9999..***************************9.****************************************************9..9 PP
   S: 235  erlvsgs..sDgsiriWdlrsgdllktlq 261
           ++lvs+s   D s+r+Wdl +g +   l+
   Q: 670  NILVSASapPDTSLRVWDLNTGSCRDILK 698
           9999999779*************987765 PP

Organism

Schizosaccharomyces pombe

Functional Description
(View)

Involved in maintenance of ploidy through proteasome dependent degradation of CDK inhibitor rum1 and S-phase initiator cdc18. Functions as a recognition factor for rum1 and cdc18, which are subsequently ubiquitinated and targeted to the 26S proteasome for degradation. Together with pop2, required for cig2 instability during G2 and M phase and cig2 degradation in exponentially growing cells. Regulates cell-cycle progression under starvation through the rum1 protein.

Protein Sequence
(Fasta)

MNEKKKDSLS VLDSNEFFGE TTMVSPSIDV SSSPRPNVER FSPCSTKKDL LEGNNIMTRI 60
PEELSRVSLQ FDSKGSQQSM IFTNNRCLSD KENLENLQNL LYLHCDLNRP HLSCELPSEH 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

TTAAAATTCA TTGCCAAGAG TTTATGGTCT TTACGATCTA TCTCGCTGAA CTTCAAAAAA 60
ACCAAGAAAA AGTATTGCTC TAATGCATTA AGACGATAAC TATTTGCAAA ATAATTTCAT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0833--Ubl conjugation pathway
KW-0853--WD repeat

Interpro

IPR001810--F-box_dom
IPR020472--G-protein_beta_WD-40_rep
IPR015943--WD40/YVTN_repeat-like_dom
IPR001680--WD40_repeat
IPR019775--WD40_repeat_CS
IPR017986--WD40_repeat_dom

PROSITE

PS50181--FBOX
PS00678--WD_REPEATS_1
PS50082--WD_REPEATS_2
PS50294--WD_REPEATS_REGION

Pfam

PF12937--F-box-like
PF00400--WD40

PRINTS

PR00320--GPROTEINBRPT

SMART

SM00256--FBOX
SM00320--WD40

Gene Ontology

GO:0005634--C:nucleus
GO:0019005--C:SCF ubiquitin ligase complex
GO:0030674--F:protein binding, bridging
GO:0000747--P:conjugation with cellular fusion
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

spo:SPBC1718.01

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Cin-025516	Ciona intestinalis	26.91	2.00e-36	144.00
IUUC-Cne-027018	Cryptococcus neoformans	34.33	1.00e-101	362.00
IUUC-Gac-042423	Gasterosteus aculeatus	29.78	1.00e-55	208.00
IUUC-Mla-058993	Melampsora laricipopulina	37.35	2.00e-94	337.00
IUUC-Ont-071489	Oreochromis niloticus	29.06	8.00e-58	216.00
IUUC-Pfo-096289	Poecilia formosa	30.06	3.00e-61	227.00
IUUC-Pgr-103612	Puccinia graminis	41.52	9.00e-85	306.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence