iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Sce-106222

Ensembl Protein ID

YOR026W

UniProt Accession

P26449; D6W292; BUB3_YEAST

Genbank Protein ID

AAA34459.1; CAA60742.1; CAA99216.1; DAA10808.1

Protein Name

Cell cycle arrest protein BUB3

Genbank Nucleotide ID

M64707; X87331; Z74934; BK006948

Gene Name

BUB3; YOR026W; OR26.16

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
YOR026W	YOR026W	YOR026W

Annotation

mRNA Expression
GEO	FFGED
Protein-protein Interaction
IID	HINT	Mentha
Protein 3D Structure
PDB	MMDB
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
UBD/Beta-Prp	Beta-Prp	21070969

Classification

Family	E-value	Score	Start	End
E3 adaptor/Cullin RING/DCX/DWD	7.70e-08	30.9	253	285
UBD/Beta-Prp	4.10e-44	149.4	3	318

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 adaptor/Cullin RING/DCX/DWD

   S: 5    svtclaf.sd.tllvsGsdDgtikvWDlrtgvl 35
           +v++++f +  ++l +++ Dg i +W+l t ++
   Q: 253  PVNSIEFsPRhKFLYTAGSDGIISCWNLQTRKK 285
           79*****955599**************988766 PP

UBD/Beta-Prp

   S: 2    iktlkghhksvitcvkfspdksllatgsrdgtikvwdvttgkl....lrsl.sgheaevtavkfspdgk..ilvtgseDrtikvwdlstgkcvhtlkghed 95
           i+++++++k++i+++k++p+ksll+ +s+dg+++v++++ + +    l+sl ++h     +++f+++++  i+v + + +++kv dl  ++ +++l+++e+
   Q: 3    IVQIEQAPKDYISDIKIIPSKSLLLITSWDGSLTVYKFDIQAKnvdlLQSLrYKHP--LLCCNFIDNTDlqIYVGTVQGEILKV-DLIGSPSFQALTNNEA 100
           7899************************************9999999999989988..*********9999*************.**************** PP
   S: 96   svlslsfspsg.dklvsGshdrtirv.....wdletgqcleetlsghdgvvncvvvhpdg.nllvsGslDrtvrlWdl.....ktgkllrtltgghtdsvy 184
           ++  ++++++g dkl+++s+d  i+v     +      + + ++s++++v n + +++++ + l+ G+++++v++++l      +g++ +   +++ ++v 
   Q: 101  NLGICRICKYGdDKLIAASWDGLIEVidprnYGDGVIAVKN-LNSNNTKVKNKIFTMDTNsSRLIVGMNNSQVQWFRLplcedDNGTIEESGLKYQIRDVA 200
           *********999**************888888888888887.******************9*****************99998888888888999****** PP
   S: 185  slsfdsngkelvsgsldgtvklwdlq....tgecvrtlnght.........dnvksvrfspnderlvsgssDgsiriWdlrsgdllktlqlhn.ssvisva 271
            l  +++g  + s++    v+++d q    +++   ++++h+          +v+s++fsp++++l++++sDg i++W+l++++ +k++++ n +sv+++a
   Q: 201  LLPKEQEGYACSSIDGRVAVEFFDDQgddyNSSKRFAFRCHRlnlkdtnlaYPVNSIEFSPRHKFLYTAGSDGIISCWNLQTRKKIKNFAKFNeDSVVKIA 301
           ******************************88889*********************************************************99******* PP
   S: 272  vlsfgdnvlvlgsedskvni 291
           +   +dn+l+l+++d+++++
   Q: 302  C---SDNILCLATSDDTFKT 318
           *...***********99975 PP

Organism

Saccharomyces cerevisiae

Functional Description
(View)

Required for cell cycle arrest in response to loss of microtubule function. Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism.

Protein Sequence
(Fasta)

MQIVQIEQAP KDYISDIKII PSKSLLLITS WDGSLTVYKF DIQAKNVDLL QSLRYKHPLL 60
CCNFIDNTDL QIYVGTVQGE ILKVDLIGSP SFQALTNNEA NLGICRICKY GDDKLIAASW 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGCAGATAG TACAAATTGA GCAGGCCCCA AAAGACTACA TAAGCGACAT CAAAATAATC 60
CCTTCCAAGT CACTGCTTTT GATTACGTCT TGGGATGGCT CTTTAACAGT CTACAAATTC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0131--Cell cycle
KW-0181--Complete proteome
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0853--WD repeat

Interpro

IPR015943--WD40/YVTN_repeat-like_dom
IPR001680--WD40_repeat
IPR017986--WD40_repeat_dom

PROSITE

PS50082--WD_REPEATS_2
PS50294--WD_REPEATS_REGION

Pfam

PF00400--WD40

SMART

SM00320--WD40

Gene Ontology

GO:0000778--C:condensed nuclear chromosome kinetochore
GO:0033597--C:mitotic checkpoint complex
GO:0043130--F:ubiquitin binding
GO:0044774--P:mitotic DNA integrity checkpoint
GO:0007094--P:mitotic spindle assembly checkpoint

KEGG

sce:YOR026W

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ago-007870	Ashbya gossypii	39.05	3.00e-58	216.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence