iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Bra-016773

Ensembl Protein ID

Bra032482.1-P

UniProt Accession

M4EUK0; M4EUK0_BRARP

Protein Name

NEDD8-activating enzyme E1 regulatory subunit

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
Bra032482	Bra032482.1	Bra032482.1-P

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	5.00e-97	326.7	18	452

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveahees 96
           YDRQ+r+wGe +q++l++a+++l+ +g++G+E lKnLvl+Gvgs+tvvD+++ve+ +l+++F++++++vg+sKA++++++l+eln++v++++ ee+
   Q: 18   YDRQLRIWGELGQAALEEASICLLHCGPTGSEALKNLVLGGVGSFTVVDGSKVELGDLGNNFMVDERSVGQSKAKSVCAFLQELNDSVKATFIEEN 113
           ************************************************************************************************ PP
   S: 97   iteleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpn....... 181
           +++l +++  Ff++f++V++++  ++++ k++r+c++a+v l+ ++++Gl G++r  + ++t+++s++d   d++++++P+++lk++++       
   Q: 114  PDTLITTNAsFFSQFTLVIATQLVEDSMVKLDRICREANVMLVFARSYGLSGFLR--VSEHTVIDSKPDhflDDLRLNNPWPELKSFVEtidlnvs 207
           ******999*********************************************5..579***************************999999999 PP
   S: 182  ..aaehtiewavlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfecdivtkskspfwv 274
             +a+++i+++v++ k+++e+a++++++ ++ +eek+e+kd+vks++ s+de+n++eaie+a+k fa++ i+s i+q+ ++ +c ++++++s+fwv
   Q: 208  dpVAHKHIPYVVILVKMADEWAQSHSGNLPSIREEKKEFKDLVKSKMISMDEDNYKEAIEAAFKVFAPRGISSeIQQICND-TCSELSSNSSDFWV 302
           99*******************************************************************************.9************* PP
   S: 275  ...........................................................................sfcsnaealq........... 284
                                                                                      sfc+na++l+           
   Q: 303  mvaalkefisnegdgeaplegsipdmtsstehyinlqkiyqakaeadvlvmekrvrnilkkigrdpssiskpaikSFCKNARKLKvcryrmvedef 398
           *************************************************************************************88888887777 PP
   S: 285  vpekvekdeevvkasap.....lylllralerfekkkgrkpgelsfekdddsavdl 335
           ++++v++ ++ ++ +++     +y+llra++rf +++++ pg+++  +d+d+  +l
   Q: 399  SNPSVTEIQKCLAGEDYsgamgFYVLLRAVDRFTANYNKFPGQFDAGMDEDI--SL 452
           779999999988888889*********************************5..33 PP

Organism

Brassica rapa

Functional Description
(View)

Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.

Protein Sequence
(Fasta)

MPPMKKEEAT MSEPKTKYDR QLRIWGELGQ AALEEASICL LHCGPTGSEA LKNLVLGGVG 60
SFTVVDGSKV ELGDLGNNFM VDERSVGQSK AKSVCAFLQE LNDSVKATFI EENPDTLITT 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGCCTCCGA TGAAGAAGGA AGAGGCAACA ATGTCAGAGC CTAAAACCAA GTACGATCGT 60
CAACTCAGGT ACACACACTA CCCTCCTCCT CAAATCACTC ATCGAAGGCT TCTTCTCATG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0019781--F:NEDD8 activating enzyme activity
GO:0045116--P:protein neddylation

Orthology

iUUCD ID

Species

Identity

E-value

Score

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence