PTM - CPLM Gene Name UniProt ID Type Position Code UPF1; KIAA0221; RENT1 Q92900 Acetylation 592 K UPF1; KIAA0221; RENT1 Q92900 Acetylation 786 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 439 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 264 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 339 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 378 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 386 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 587 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 638 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 688 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 790 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 926 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 929 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 793 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 797 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 892 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 845 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 321 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 467 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 604 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 544 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 786 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 455 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 340 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 888 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 282 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 220 K UPF1; KIAA0221; RENT1 Q92900 Ubiquitination 551 K

PTM - dbPAF Gene Name UniProt ID Position Type Species UPF1; KIAA0221; RENT1 Q92900 10 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1084 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1089 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1100 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1107 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1110 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1111 T Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1112 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1118 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1124 T Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1127 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 1129 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 113 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 114 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 136 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 139 T Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 215 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 247 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 28 T Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 31 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 344 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 346 T Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 350 T Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 46 T Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 475 T Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 485 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 488 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 565 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 568 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 595 T Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 692 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 879 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 946 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 956 S Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 958 Y Homo sapiens(Human) UPF1; KIAA0221; RENT1 Q92900 970 Y Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q92900 K386-ac Acetylation GDLAPLWkGIGHVIK Q92900 K592-ac Acetylation LQkLQQLkDEtGELS Q92900 K786-ac Acetylation TEAANVEkITTkLLk Q92900 R433-m1 Methylation WKSTSFDrMQSALkT Q92900 R811-m1 Methylation ITPYEGQrSYLVQYM Q92900 R939-m1 Methylation NTINPGArFMTTAMy Q92900 R1019-m1 Methylation ANGPAAGrGTPKGKT Q92900 S10-p Phosphorylation VEAYGPSsQTLTFLD Q92900 T28-p Phosphorylation AELLGADtQGsEFEF Q92900 S31-p Phosphorylation LGADtQGsEFEFTDF Q92900 Y113-p Phosphorylation EEDEEDTyyTKDLPI Q92900 Y114-p Phosphorylation EDEEDTyyTKDLPIH Q92900 Y136-p Phosphorylation HDPACVVyCNTSKKW Q92900 T151-p Phosphorylation FCNGRGNtSGSHIVN Q92900 S247-p Phosphorylation SWLVKIPsEQEQLRA Q92900 Y451-p Phosphorylation DETSVSGyIYHKLLG Q92900 T475-p Phosphorylation CQLPKRFtAQGLPDL Q92900 Y488-p Phosphorylation DLNHSQVyAVKTVLQ Q92900 Y518-p Phosphorylation VTSATIVyHLARQGN Q92900 S565-p Phosphorylation KSREAIDsPVSFLAL Q92900 T595-p Phosphorylation LQQLkDEtGELSSAD Q92900 S692-p Phosphorylation KAAkAGLsQSLFERL Q92900 Y879-p Phosphorylation VALTRARyGVIIVGN Q92900 Y946-p Phosphorylation rFMTTAMyDAREAII Q92900 S956-p Phosphorylation REAIIPGsVyDRSSQ Q92900 Y958-p Phosphorylation AIIPGsVyDRSSQGR Q92900 Y970-p Phosphorylation QGRPSSMyFQTHDQI Q92900 S1084-p Phosphorylation QMSQPGLsQPELsQD Q92900 S1089-p Phosphorylation GLsQPELsQDSYLGD Q92900 S1100-p Phosphorylation YLGDEFKsQIDVALs Q92900 S1107-p Phosphorylation sQIDVALsQDstyQG Q92900 S1110-p Phosphorylation DVALsQDstyQGERA Q92900 T1111-p Phosphorylation VALsQDstyQGERAy Q92900 Y1112-p Phosphorylation ALsQDstyQGERAyQ Q92900 Y1118-p Phosphorylation tyQGERAyQHGGVTG Q92900 S1127-p Phosphorylation HGGVTGLsQy_____ Q92900 Y1129-p Phosphorylation GVTGLsQy_______ Q92900 K200-ub Ubiquitination LLGFIPAkADSVVVL Q92900 K264-ub Ubiquitination ITAQQINkLEELWKE Q92900 K282-ub Ubiquitination ATLEDLEkPGVDEEP Q92900 K321-ub Ubiquitination ADYDKKLkESQTQDN Q92900 K378-ub Ubiquitination DEICLRYkGDLAPLW Q92900 K386-ub Ubiquitination GDLAPLWkGIGHVIK Q92900 K439-ub Ubiquitination DrMQSALkTFAVDET Q92900 K551-ub Ubiquitination KIHQTGLkVVRLCAK Q92900 K587-ub Ubiquitination DSMPELQkLQQLkDE Q92900 K604-ub Ubiquitination ELSSADEkRYRALKR Q92900 K638-ub Ubiquitination AGDPRLAkMQFRSIL Q92900 K688-ub Ubiquitination VMCKKAAkAGLsQSL Q92900 K786-ub Ubiquitination TEAANVEkITTkLLk Q92900 K790-ub Ubiquitination NVEkITTkLLkAGAk Q92900 K793-ub Ubiquitination kITTkLLkAGAkPDQ Q92900 K797-ub Ubiquitination kLLkAGAkPDQIGII Q92900 K926-ub Ubiquitination ESLMQFSkPRkLVNT Q92900 K929-ub Ubiquitination MQFSkPRkLVNTINP

PTM - dbPTM UniProt ID Position Code Type Resources References Q92900 1089 S Phosphorylation Swiss-Prot 1010711 11544179;16452507 Q92900 1107 S Phosphorylation Swiss-Prot 1010711 11544179;16452507;17525332;18669648;19413330;19690332 Q92900 1127 S Phosphorylation Swiss-Prot 1010711 17525332;19690332 Q92900 1129 Y Phosphorylation Swiss-Prot 1010711 18220336 Q92900 1107 S Phosphorylation PhosphoELM.10011 18669648;17525332;17081983 Q92900 1110 S Phosphorylation PhosphoELM.10011 18669648;17081983 Q92900 1127 S Phosphorylation PhosphoELM.10011 18669648;18220336;17525332 Q92900 1111 T Phosphorylation PhosphoELM.10011 18669648 Q92900 1112 Y Phosphorylation PhosphoELM.10011 18669648 Q92900 1129 Y Phosphorylation PhosphoELM.10011 18220336 Q92900 264 K Ubiquitylation Phosphositeplus.1010730 21890473 Q92900 282 K Ubiquitylation Phosphositeplus.1010730 21906983 Q92900 321 K Ubiquitylation Phosphositeplus.1010730 21906983 Q92900 378 K Ubiquitylation Phosphositeplus.1010730 21890473 Q92900 386 K Ubiquitylation Phosphositeplus.1010730 21890473 Q92900 439 K Ubiquitylation Phosphositeplus.1010730 21890473 Q92900 587 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q92900 604 K Ubiquitylation Phosphositeplus.1010730 21906983 Q92900 638 K Ubiquitylation Phosphositeplus.1010730 21890473 Q92900 688 K Ubiquitylation Phosphositeplus.1010730 21890473 Q92900 790 K Ubiquitylation Phosphositeplus.1010730 21890473 Q92900 797 K Ubiquitylation Phosphositeplus.1010730 21906983 Q92900 926 K Ubiquitylation Phosphositeplus.1010730 21890473 Q92900 1084 S Phosphorylation Phosphositeplus.1010730 11544179 Q92900 1089 S Phosphorylation Phosphositeplus.1010730 11544179;21965535 Q92900 1107 S Phosphorylation Phosphositeplus.1010730 11544179;17525332;18669648;19415658;19664994;19664995;19690332;20068231;20164059;20363803;21406692;21659604;21965535;22115753 Q92900 1110 S Phosphorylation Phosphositeplus.1010730 19415658;19690332;20068231;20164059;21406692;22115753 Q92900 1127 S Phosphorylation Phosphositeplus.1010730 11544179;17525332;18220336;19276368;19415658;19664994;19664995;19690332;20068231;21406692;21965535;22115753 Q92900 1111 T Phosphorylation Phosphositeplus.1010730 19690332 Q92900 28 T Phosphorylation Phosphositeplus.1010730 21965535 Q92900 475 T Phosphorylation Phosphositeplus.1010730 20068231 Q92900 1112 Y Phosphorylation Phosphositeplus.1010730 20562096 Q92900 1129 Y Phosphorylation Phosphositeplus.1010730 18220336;19415658 Q92900 113 Y Phosphorylation Phosphositeplus.1010730 20562096 Q92900 488 Y Phosphorylation Phosphositeplus.1010730 17360941;21394647 Q92900 946 Y Phosphorylation Phosphositeplus.1010730 20007894;21403953;21447384 Q92900 958 Y Phosphorylation Phosphositeplus.1010730 21403953 Q92900 215 S Phosphorylation HPRD 9.0 20068231 Q92900 485 S Phosphorylation HPRD 9.0 20068231 Q92900 568 S Phosphorylation HPRD 9.0 20068231 Q92900 1107 S Phosphorylation HPRD 9.0 17525332,18669648,19413330,19415658,18077418,19664995,19651622,19664994,18767875,20068231 Q92900 1110 S Phosphorylation HPRD 9.0 17081983,18669648,19415658,19651622,20068231 Q92900 1127 S Phosphorylation HPRD 9.0 17525332,18669648,19415658,18077418,19664995,19664994,20068231 Q92900 475 T Phosphorylation HPRD 9.0 20068231 Q92900 1111 T Phosphorylation HPRD 9.0 17081983,18669648,20068231 Q92900 1124 T Phosphorylation HPRD 9.0 20068231 Q92900 1112 Y Phosphorylation HPRD 9.0 18669648,20068231 Q92900 1118 Y Phosphorylation HPRD 9.0 19415658 Q92900 1129 Y Phosphorylation HPRD 9.0 19415658 Q92900 1107 S Phosphorylation SysPTM 1.1 18669648 Q92900 1110 S Phosphorylation SysPTM 1.1 17081983 Q92900 1111 T Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References UPF1 1096 S Phosphorylation 17525332,18669648,19413330,19415658,18077418,19664995,19651622,19664994,18767875,20068231 UPF1 1099 S Phosphorylation 17081983,18669648,19415658,19651622,20068231 UPF1 1100 T Phosphorylation 17081983,18669648,20068231 UPF1 1101 Y Phosphorylation 18669648,20068231 UPF1 1107 S Phosphorylation 17525332,18669648,19413330,19415658,18077418,19664995,19651622,19664994,18767875,20068231 UPF1 1110 S Phosphorylation 17081983,18669648,19415658,19651622,20068231 UPF1 1111 T Phosphorylation 17081983,18669648,20068231 UPF1 1112 Y Phosphorylation 18669648,20068231 UPF1 1113 T Phosphorylation 20068231 UPF1 1116 S Phosphorylation 17525332,18669648,19415658,18077418,19664995,19664994,20068231 UPF1 1118 Y Phosphorylation 19415658 UPF1 1124 T Phosphorylation 20068231 UPF1 1127 S Phosphorylation 17525332,18669648,19415658,18077418,19664995,19664994,20068231 UPF1 1129 Y Phosphorylation 19415658 UPF1 215 S Phosphorylation 20068231 UPF1 464 T Phosphorylation 20068231 UPF1 474 S Phosphorylation 20068231 UPF1 475 T Phosphorylation 20068231 UPF1 485 S Phosphorylation 20068231 UPF1 557 S Phosphorylation 20068231 UPF1 568 S Phosphorylation 20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q92900 1110 S 18669648 HTP Phosphorylation Q92900 1110 S 17081983 HTP Phosphorylation Q92900 1112 Y 18669648 HTP Phosphorylation Q92900 1107 S 18669648 HTP Phosphorylation Q92900 1107 S 17525332 HTP Phosphorylation Q92900 1107 S 17081983 HTP Phosphorylation Q92900 1129 Y 18220336 HTP Phosphorylation Q92900 1111 T 18669648 HTP Phosphorylation Q92900 1127 S 18669648 HTP Phosphorylation Q92900 1127 S 18220336 HTP Phosphorylation Q92900 1127 S 17525332 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q92900 10 Phosphoserine 24275569 Q92900 31 Phosphoserine 24275569 Q92900 565 Phosphoserine 23186163 Q92900 956 Phosphoserine 23186163 Q92900 1019 Omega-N-methylarginine 24129315 Q92900 1089 Phosphoserine 11544179;16452507 Q92900 1107 Phosphoserine 17525332;18669648;19690332;20068231;21406692;23186163;11544179;16452507 Q92900 1110 Phosphoserine 20068231;21406692 Q92900 1127 Phosphoserine 20068231

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type UPF1 IPI00034049 S 1107 QIDVALpSQDSTYQ phosphorylation UPF1 IPI00034049 S 1110 VALSQDpSTYQGER phosphorylation UPF1 IPI00034049 S 1127 GGVTGLpSQY____ phosphorylation UPF1 IPI00034049 S 215 CRQPCApSQSSLKD phosphorylation UPF1 IPI00034049 S 485 LPDLNHpSQVYAVK phosphorylation UPF1 IPI00034049 T 139 CVVYCNpTSKKWFC phosphorylation UPF1 IPI00034049 T 475 QLPKRFpTAQGLPD phosphorylation UPF1 IPI00034049 Y 1112 LSQDSTpYQGERAY phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 5976 111908 - K Ubiquitination 18781797 5976 111908 - K Ubiquitination 21139048 5976 111908 - K Ubiquitination 21890473 5976 111908 - K Ubiquitination 21906983 5976 111908 - K Ubiquitination 22053931 5976 111908 - K Ubiquitination 22505724 5976 111908 - K FAT10ylation 22797925 5976 111908 - K Ubiquitination 21963094 5976 111908 - K Ubiquitination 23000965 5976 111908 - K Ubiquitination 24816145 5976 111908 - K Sumoylation 25027693 5976 111908 - K Ubiquitination 25147182 5976 111908 - K Neddylation 18247557

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID UPF1 Homo sapiens (Human) 576; 627; 375; 339; 428; 264; 677; 367; 779; 915 "576,SMPELQKLQQLKD; 627,GDPRLAKMQFRSI; 375,DLAPLWKGIGHVI; 339,WDLGLNKKRIAYF; 428,RMQSALKTFAVDE; 264,TAQQINKLEELWK; 677,MCKKAAKAGLSQS; 367,EICLRYKGDLAPL; 779,VEKITTKLLKAGA; 915,SLMQFSKPRKLVN" 576:21890473; 627:21890473; 375:21890473; 339:21890473; 428:21890473; 264:21890473; 677:21890473; 367:21890473; 779:21890473; 915:21890473 UPF1 Homo sapiens (Human) 576; 627; 375; 339; 428; 264; 677; 367; 779; 915 "576,SMPELQKLQQLKD; 627,GDPRLAKMQFRSI; 375,DLAPLWKGIGHVI; 339,WDLGLNKKRIAYF; 428,RMQSALKTFAVDE; 264,TAQQINKLEELWK; 677,MCKKAAKAGLSQS; 367,EICLRYKGDLAPL; 779,VEKITTKLLKAGA; 915,SLMQFSKPRKLVN" 576:21890473; 627:21890473; 375:21890473; 339:21890473; 428:21890473; 264:21890473; 677:21890473; 367:21890473; 779:21890473; 915:21890473 UPF1 Homo sapiens (Human) 926; 790; 339; 282; 797; 587; 378; 439; 321; 386; 264; 638; 604; 688 "926,SLMQFSKPRKLVN; 790,VEKITTKLLKAGA; 339,WDLGLNKKRIAYF; 282,TLEDLEKPGVDEE; 797,LLKAGAKPDQIGI; 587,SMPELQKLQQLKD; 378,EICLRYKGDLAPL; 439,RMQSALKTFAVDE; 321,DYDKKLKESQTQD; 386,DLAPLWKGIGHVI; 264,TAQQINKLEELWK; 638,GDPRLAKMQFRSI; 604,LSSADEKRYRALK; 688,MCKKAAKAGLSQS" "926:21890473; 790:21890473; 339:21890473; 282:21906983; 797:21906983; 587:21890473,21906983; 378:21890473; 439:21890473,22053931; 321:21906983; 386:21890473; 264:21890473; 638:21890473,22053931; 604:21906983; 688:21890473" UPF1 Homo sapiens (Human) 576; 375; 339; 786; 282; 677; 915; 593; 627; 428; 321; 264; 367; 779 "576,SMPELQKLQQLKD; 375,DLAPLWKGIGHVI; 339,WDLGLNKKRIAYF; 786,LLKAGAKPDQIGI; 282,TLEDLEKPGVDEE; 677,MCKKAAKAGLSQS; 915,SLMQFSKPRKLVN; 593,LSSADEKRYRALK; 627,GDPRLAKMQFRSI; 428,RMQSALKTFAVDE; 321,DYDKKLKESQTQD; 264,TAQQINKLEELWK; 367,EICLRYKGDLAPL; 779,VEKITTKLLKAGA" "576:21890473,21906983; 375:21890473; 339:21890473; 786:21906983; 282:21906983; 677:21890473; 915:21890473; 593:21906983; 627:21890473; 428:21890473; 321:21906983; 264:21890473; 367:21890473; 779:21890473" UPF1 Homo sapiens (Human) 638; 439 "638,GDPRLAKMQFRSI; 439,RMQSALKTFAVDE" "638:22053931; 439:21139048,22053931"