PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus MOV10 RNF216 22658674 biogrid exp G G G G G G G G G G G G G G G G G G G G G G KIR2DL4 RNF216 21270397 innatedb,biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G TLR5 RNF216 15107846 hprd,biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G TIRAP RNF216 16968706 innatedb,biogrid exp G GP G G G GP G GP GP G GP G G G G GP GP GP GP G GP G GP GP GP G GP GP G BRCA1 RNF216 22990118 biogrid exp P P G GP P P P P P P P GP P P P P P P P P P P GP RNF216 RNF216 16169070 hprd,biogrid,i2d,mint,intact exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G TLR4 RNF216 15107846 innatedb,hprd,biogrid exp G GP G G GP GP G G G G G GP G GP G G G GP G GP G UBE2C RNF216 15107846 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G UBE2H RNF216 15107846 biogrid exp GP GP G G GP GP G GP G G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G TOLLIP RNF216 20936779 i2d exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G TLR3 RNF216 15107846 hprd,biogrid exp GP GP P G P GP GP GP GP G GP P GP GP GP GP G GP G GP GP G AGFG1 RNF216 11854271 hprd exp GP GP G G GP GP G G GP G GP GP GP GP G GP GP GP GP G GP GP G G GP GP GP GP G UBE2L6 RNF216 15107846 hprd,biogrid exp GP GP G G GP GP G G GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G RNF216 NXF1 22658674 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G RNF216 RNF11 15231748 biogrid,i2d,mint,intact exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G XPO1 RNF216 26673895 biogrid exp GP GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G UBE2L3 RNF216 15107846, 25402006 hprd,biogrid exp, ortho, pred GP G G G G GP G GP GP G GP G G G G GP GP G GP G G G GP GP GP G GP GP G TLR9 RNF216 15107846, 25402006 innatedb,hprd,biogrid exp, pred GP G G G GP GP GP GP G GP P GP GP G P GP GP P G P P GP GP GP GP GP GP G RIPK1 RNF216 11854271,16968706, 25402006 hprd,biogrid exp, pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G TRAF3 RNF216 19893624, 25402006 innatedb,biogrid exp, pred G GP G G GP GP G G G G GP GP GP GP G GP GP GP GP G GP G GP G GP G GP GP G TICAM1 RNF216 16968706, 25402006 biogrid exp, pred GP GP G P G GP GP GP GP GP GP G GP P GP GP GP GP GP GP GP GP GP P G RNF216 TNFRSF11A 25402006 pred GP P G GP P P GP P P GP G GP P P G P G G P GP GP P G TRAF2 RNF216 25402006 pred P P P P P P P P P P P P P P P P P P P GP GP P CA11 RNF216 25402006 pred GP G G G GP GP G G GP G GP G G G G GP GP G GP G GP GP G G GP GP GP GP G CCDC50 RNF216 25402006 pred GP GP G G GP GP G G G G GP G GP GP G G GP GP G G GP G G G G GP GP GP G RNF216 IKBKG 25402006 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G BTK RNF216 25402006 pred G G GP G G GP GP G G GP G BIRC3 RNF216 25402006 pred GP G GP G G GP P GP G GP G GP G GP P G GP GP GP GP GP NUP62 RNF216 25402006 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP P G GP GP GP GP GP GP GP GP G TNFSF14 RNF216 25402006 pred GP P P P P P P GP GP G P P G P P P P P G P P EDA2R RNF216 25402006 pred P P P G P P G P P G GP G G GP P P P P G OPTN RNF216 25402006 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G ZBP1 RNF216 25402006 pred P P P GP P P G RNF216 RIPK3 25402006 pred GP P G GP P GP GP G P P GP GP G GP P P GP G GP GP GP GP GP GP GP P BIRC6 RNF216 16082366 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G TRAF1 RNF216 25402006 pred G G G G GP GP G G GP G GP G G GP G G GP G G G G G G G GP G G G CRADD RNF216 25402006 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP G GP G GP GP G GP GP G GP GP G TRIM38 RNF216 25402006 pred GP GP G GP P G GP GP GP GP GP GP G GP GP GP GP GP GP GP GP GP GP GP P G ZMYND11 RNF216 25402006 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G TNFRSF4 RNF216 25402006 pred P GP GP G BIRC2 RNF216 25402006 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP G GP G GP G GP GP GP GP GP GP G MAP3K14 RNF216 25402006 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G MAVS RNF216 25402006 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G RNF216 TBK1 25402006 pred GP GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G CD27 RNF216 25402006 pred G G G GP G G G G G G GP G G G G G G G G G G GP G G TRPC4AP RNF216 25402006 pred GP GP G G GP GP G GP GP G GP GP GP G G GP GP GP GP G GP G GP G GP GP GP GP G TRAF5 RNF216 25402006 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP G GP G GP GP GP GP GP GP GP GP G TMEM173 RNF216 25402006 pred GP G G G GP GP G GP GP G G G GP GP G G GP G GP G GP GP GP G G GP GP GP G RIPK2 RNF216 25402006 pred GP GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP GP GP GP GP G G GP G RNF135 RNF216 25402006 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP G GP G GP GP GP GP GP G GP GP G OTUD5 RNF216 25402006 pred GP GP G G G GP G G GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G TRADD RNF216 25402006 pred GP GP G G GP GP G G GP G GP G GP GP G G GP G G G GP GP GP G G GP GP GP G TNFRSF9 RNF216 25402006 pred GP G G G GP G GP GP P G P G GP G GP G P GP GP GP GP G TNFRSF8 RNF216 25402006 pred GP G G G G G G G GP G G G G GP G G G G G G G G G G GP G G FADD RNF216 25402006 pred GP GP G G G GP G GP GP G GP G GP GP G GP GP G GP G GP G GP GP GP GP GP GP G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q9NWF9 Q9Y3C5 two hybrid 15231748 physical association X 2 Q9NWF9 Q8ZBZ9 two hybrid pooling approach 20711500 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B UBE2L3 RNF216 MI:0018(two hybrid)|MI:0018(two hybrid) 15107846 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|- (biogrid)|MI:0468(hprd) BIOGRID_86604|HPRD_12619 prey|- bait|- TICAM1 RNF216 MI:0004(affinity chromatography technology) 16968706 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_122421 bait prey RNF216 RNF11 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 15231748 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association) (intact)|MI:0471(mint)|(biogrid)|MI:0471(mint) UniProt_78527|MINT_60203|BIOGRID_259441|MINT_31438 prey|prey|prey|prey bait|bait|bait|bait BRCA1 RNF216 MI:0004(affinity chromatography technology) 22990118 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_245662 bait prey UBE2L6 RNF216 MI:0018(two hybrid)|MI:0018(two hybrid) 15107846 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) -|MI:0915(physical association) MI:0468(hprd)|(biogrid) HPRD_12741|BIOGRID_86606 -|prey -|bait TLR4 RNF216 MI:0018(two hybrid)|MI:0018(two hybrid) 15107846 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) -|MI:0407(direct interaction) MI:0468(hprd)|(biogrid) HPRD_11921|BIOGRID_77906 -|prey -|bait TLR5 RNF216 MI:0018(two hybrid)|MI:0018(two hybrid) 15107846 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) -|MI:0407(direct interaction) MI:0468(hprd)|(biogrid) HPRD_11925|BIOGRID_77907 -|prey -|bait UBE2C RNF216 MI:0018(two hybrid) 15107846 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_86605 prey bait TRAF3 RNF216 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 19893624 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid) BIOGRID_153212|BIOGRID_153208 prey|bait bait|prey UBE2H RNF216 MI:0018(two hybrid) 15107846 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_86603 prey bait TIRAP RNF216 MI:0004(affinity chromatography technology) 16968706 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_122420 prey bait TLR3 RNF216 MI:0018(two hybrid)|MI:0018(two hybrid) 15107846 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) -|MI:0407(direct interaction) MI:0468(hprd)|(biogrid) HPRD_11915|BIOGRID_77905 -|prey -|bait SUMO2 RNF216 MI:0004(affinity chromatography technology) 21693764 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_165393 bait prey RIPK1 RNF216 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0493(in vivo) 11854271,16968706 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|- (biogrid)|(biogrid)|(biogrid)|(biogrid)|MI:0468(hprd) BIOGRID_101575|BIOGRID_101576|BIOGRID_122419|BIOGRID_122423|HPRD_12381 bait|prey|prey|bait|- prey|bait|bait|prey|- KIR2DL4 RNF216 MI:0018(two hybrid)|MI:0018(two hybrid) 21270397 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(biogrid) BIOGRID_153016|BIOGRID_153017 bait|bait prey|prey TLR9 RNF216 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 15107846 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) -|MI:0407(direct interaction)|MI:0407(direct interaction) MI:0468(hprd)|(biogrid)|(biogrid) HPRD_13773|BIOGRID_86598|BIOGRID_77999 -|bait|prey -|prey|bait AGFG1 RNF216 MI:0493(in vivo) 11854271 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) - MI:0468(hprd) HPRD_7908 - - UBC RNF216 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0415(enzymatic study)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 21139048,21890473,21906983,21963094,21987572,22505724,23000965 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid) BIOGRID_177273|BIOGRID_167842|BIOGRID_174776|BIOGRID_224444|BIOGRID_163794|BIOGRID_270326|BIOGRID_166465 bait|bait|bait|bait|bait|bait|bait prey|prey|prey|prey|prey|prey|prey

PPI - HINT Gene A Gene B PMID Method Quality RIPK1 RNF216 11854271,11854271,16968706 0018,0004,0004 LC RIPK1 RNF216 11854271,11854271,16968706 0018,0004,0004 LC TLR4 RNF216 15107846 0018 LC TLR3 RNF216 15107846 0018 LC TLR5 RNF216 15107846 0018 LC BRCA1 RNF216 22990118 0018 LC RIPK1 RNF216 11854271,11854271 0018,0004 LC KIR2DL4 RNF216 21270397,21270397 0018,0004 LC TLR9 RNF216 15107846 0018 LC RNF216 RNF216 16169070,16169070 0018,0398 HT RNF216 RNF11 15231748 0018 HT

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q9NWF9 Q9HCE1 RNF216 MOV10 0.126 22658674 Q9NWF9 Q9NR96 RNF216 TLR9 0.523 15107846 Q9NWF9 O00206 RNF216 TLR4 0.309 15107846 Q9NWF9 O00762 RNF216 UBE2C 0.21 15107846 Q9NWF9 O14933 RNF216 UBE2L6 0.396 unassigned1304,15107846 Q9NWF9 O15455 RNF216 TLR3 0.309 15107846 Q9NWF9 O60602 RNF216 TLR5 0.309 15107846 Q9NWF9 P12504 RNF216 VIF 0.569 15367624,12600646 Q9NWF9 P58753 RNF216 TIRAP 0.21 16968706 Q9NWF9 P62256 RNF216 UBE2H 0.21 15107846 Q9NWF9 Q7LC44 RNF216 ARC 0.376 24945773 Q9NWF9 Q8ZBZ9 RNF216 DINB 0.126 20711500 Q9NWF9 Q8IUC6 RNF216 TICAM1 0.21 16968706 Q9NWF9 Q6P5F9 RNF216 XPO1 0.126 26673895 Q9NWF9 P69721 RNF216 VIF 0.74 12600646,15367624 Q9NWF9 P69720 RNF216 VIF 0.74 12600646,15367624 Q9NWF9 P69722 RNF216 VIF 0.74 12600646,15367624 Q9NWF9 Q9NWF9 RNF216 RNF216 0.332 16169070 Q9NWF9 P69723 RNF216 VIF 0.74 12600646,15367624 Q9NWF9 Q13546 RNF216 RIPK1 0.659 16968706,11854271 Q9Y3C5 Q9NWF9 RNF11 RNF216 0.332 15231748 Q9NWF9 Q8TF42 RNF216 UBASH3B 0.236 unassigned1304 Q9NWF9 Q92567 RNF216 FAM168A 0.236 unassigned1304 Q9NWF9 Q96CV9 RNF216 OPTN 0.236 unassigned1304 Q9NWF9 Q14149 RNF216 MORC3 0.236 unassigned1304 Q9NWF9 Q99706 RNF216 KIR2DL4 0.454 21270397 Q9NWF9 Q9BSL1 RNF216 UBAC1 0.236 unassigned1304 Q9NWF9 P21580 RNF216 TNFAIP3 0.236 unassigned1304 Q9NWF9 J3KQ25 RNF216 ZFAND2A {ECO:0000313|ENSEMBL:ENSP00000386031} 0.236 unassigned1304 Q9NWF9 P45974 RNF216 USP5 0.236 unassigned1304 Q9NWF9 Q5VVQ6 RNF216 YOD1 0.236 unassigned1304 Q9NWF9 P57075 RNF216 UBASH3A 0.236 unassigned1304 Q9NWF9 O75808 RNF216 CAPN15 0.236 unassigned1304 Q9NWF9 P68036 RNF216 UBE2L3 0.396 unassigned1304,15107846 Q9NWF9 P38398 RNF216 BRCA1 0.183 22990118 Q9UBU9 Q9NWF9 NXF1 RNF216 0.126 22658674 Q9NWF9 Q14457 RNF216 BECN1 0.376 25484083 Q9NWF9 Q13114 RNF216 TRAF3 0.376 19893624

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 TLR4 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database UBE2C RNF216 MI:0045 experimental interaction detection MI:0461 interaction database UBE2L6 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database XPO1 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database TLR3 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database TLR5 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database UBC RNF216 MI:0045 experimental interaction detection MI:0461 interaction database BRCA1 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database SUMO3 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database TIRAP RNF216 MI:0045 experimental interaction detection MI:0461 interaction database SUMO2 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database UBE2H RNF216 MI:0045 experimental interaction detection MI:0461 interaction database UBE2L3 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database TRAF3 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database RIPK1 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database BECN1 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database TICAM1 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database KIR2DL4 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database MOV10 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database TLR9 RNF216 MI:0045 experimental interaction detection MI:0461 interaction database RNF216 NXF1 MI:0045 experimental interaction detection MI:0461 interaction database RNF216 RNF11 MI:0045 experimental interaction detection MI:0461 interaction database