PTM - CPLM Gene Name UniProt ID Type Position Code RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Acetylation 425 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 448 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 641 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 351 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 362 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 425 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 619 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 485 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 654 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 487 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 100 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 391 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 666 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 765 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 773 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 584 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 459 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 508 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 398 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 658 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 413 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 538 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 684 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 777 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 589 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 406 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 635 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 430 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 414 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 354 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 427 K RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 Ubiquitination 554 K

PTM - dbPAF Gene Name UniProt ID Position Type Species RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 232 S Homo sapiens(Human) RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 249 S Homo sapiens(Human) RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 35 S Homo sapiens(Human) RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 387 S Homo sapiens(Human) RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 401 Y Homo sapiens(Human) RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 419 S Homo sapiens(Human) RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 437 Y Homo sapiens(Human) RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 706 S Homo sapiens(Human) RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 710 Y Homo sapiens(Human) RNF216; TRIAD3; UBCE7IP1; ZIN Q9NWF9 719 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9NWF9 K425-ac Acetylation LsPETSGkRKKRkQM Q9NWF9 S35-p Phosphorylation RDGPITIsDSSDEER Q9NWF9 S249-p Phosphorylation FEDQQLAsDDEEPGP Q9NWF9 S340-p Phosphorylation DRIIINPsSSLLASQ Q9NWF9 S387-p Phosphorylation ADFKVLSsQDIkWAL Q9NWF9 Y401-p Phosphorylation LHELKGHyAITRKAL Q9NWF9 S419-p Phosphorylation IKKWQELsPETSGkR Q9NWF9 Y437-p Phosphorylation kQMNQYSyIDFKFEQ Q9NWF9 S607-p Phosphorylation DELVRCPsCSFPALL Q9NWF9 S706-p Phosphorylation MCYLCRVsINGyDHF Q9NWF9 Y710-p Phosphorylation CRVsINGyDHFCQHP Q9NWF9 S719-p Phosphorylation HFCQHPRsPGAPCQE Q9NWF9 K100-sm Sumoylation GQTEREPkPGPSHNQ Q9NWF9 K351-sm Sumoylation LASQDETkLPkIDFF Q9NWF9 K354-sm Sumoylation QDETkLPkIDFFDYS Q9NWF9 K448-sm Sumoylation KFEQGDIkIEKRMFF Q9NWF9 K485-sm Sumoylation EQEFYEQkIKEMAEH Q9NWF9 K589-sm Sumoylation LYkYYERkAEEEVAA Q9NWF9 K619-sm Sumoylation ALLDSDVkRFSCPNP Q9NWF9 K658-sm Sumoylation LAEKDDIkYRTSIEE Q9NWF9 K666-sm Sumoylation YRTSIEEkMTAARIR Q9NWF9 K777-sm Sumoylation PLEkPVEkVQRVEAL Q9NWF9 K100-ub Ubiquitination GQTEREPkPGPSHNQ Q9NWF9 K351-ub Ubiquitination LASQDETkLPkIDFF Q9NWF9 K354-ub Ubiquitination QDETkLPkIDFFDYS Q9NWF9 K362-ub Ubiquitination IDFFDYSkLTPLDQR Q9NWF9 K391-ub Ubiquitination VLSsQDIkWALHELK Q9NWF9 K425-ub Ubiquitination LsPETSGkRKKRkQM Q9NWF9 K430-ub Ubiquitination SGkRKKRkQMNQYSy Q9NWF9 K485-ub Ubiquitination EQEFYEQkIKEMAEH Q9NWF9 K584-ub Ubiquitination LPQTILYkYYERkAE Q9NWF9 K619-ub Ubiquitination ALLDSDVkRFSCPNP Q9NWF9 K658-ub Ubiquitination LAEKDDIkYRTSIEE Q9NWF9 K666-ub Ubiquitination YRTSIEEkMTAARIR Q9NWF9 K765-ub Ubiquitination KNGENTFkRIGPPLE Q9NWF9 K773-ub Ubiquitination RIGPPLEkPVEkVQR Q9NWF9 K777-ub Ubiquitination PLEkPVEkVQRVEAL

PTM - dbPTM UniProt ID Position Code Type Resources References Q9NWF9 35 S Phosphorylation Swiss-Prot 1010711 17924679 Q9NWF9 100 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NWF9 351 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NWF9 362 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NWF9 391 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9NWF9 425 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NWF9 485 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NWF9 584 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9NWF9 666 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NWF9 765 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NWF9 773 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NWF9 387 S Phosphorylation Phosphositeplus.1010730 17525332 Q9NWF9 419 S Phosphorylation Phosphositeplus.1010730 19664994;22115753 Q9NWF9 401 Y Phosphorylation Phosphositeplus.1010730 17525332 Q9NWF9 35 S Phosphorylation HPRD 9.0 17924679 Q9NWF9 419 S Phosphorylation HPRD 9.0 19664994

PTM - HPRD Symbol Position Code Type References RNF216 35 S Phosphorylation 17924679 RNF216 41 S Phosphorylation 19664994 RNF216 419 S Phosphorylation 19664994 RNF216 476 S Phosphorylation 19664994

PTM - UniProt UniProt ID Position Type PMIDs Q9NWF9 419 Phosphoserine 23186163

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 54476 119981 - K Ubiquitination 21987572 54476 119981 - K Sumoylation 21693764 54476 119981 - K Ubiquitination 21963094 54476 119981 - K Ubiquitination 21139048 54476 119981 - K Ubiquitination 21890473 54476 119981 - K Ubiquitination 21906983 54476 119981 - K Ubiquitination 22505724 54476 119981 - K Ubiquitination 23000965 54476 119981 - K Ubiquitination 25147182 54476 119981 - K Sumoylation 25391492

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID RNF216 Homo sapiens (Human) 478; 577; 204; 237; 174; 203; 397; 163; 297; 585 "478,RTSIEEKMTAARI; 577,NGENTFKRIGPPL; 204,LSSQDIKWALHEL; 237,SPETSGKRKKRKQ; 174,DFFDYSKLTPLDQ; 203,LSSQDIKWALHEL; 397,PQTILYKYYERKA; 163,ASQDETKLPKIDF; 297,QEFYEQKIKEMAE; 585,IGPPLEKPVEKVQ" 478:21906983; 577:21906983; 204:21890473; 237:21906983; 174:21906983; 203:21906983; 397:21890473; 163:21906983; 297:21906983; 585:21906983 RNF216 Homo sapiens (Human) 100 "100,QTEREPKPGPSHN" 100:21906983 RNF216 Homo sapiens (Human) 641; 448 "641,PQTILYKYYERKA; 448,LSSQDIKWALHEL" 641:21890473; 448:21890473 RNF216 Homo sapiens (Human) 204; 397 "204,LSSQDIKWALHEL; 397,PQTILYKYYERKA" 204:21890473; 397:21890473 RNF216 Homo sapiens (Human) 584; 391 "584,PQTILYKYYERKA; 391,LSSQDIKWALHEL" 584:21890473; 391:21890473 RNF216 Homo sapiens (Human) 288; 395; 204; 397; 387; 13; 47; 107 "288,RTSIEEKMTAARI; 395,IGPPLEKPVEKVQ; 204,LSSQDIKWALHEL; 397,PQTILYKYYERKA; 387,NGENTFKRIGPPL; 13,LSSQDIKWALHEL; 47,SPETSGKRKKRKQ; 107,QEFYEQKIKEMAE" 288:21906983; 395:21906983; 204:21890473; 397:21890473; 387:21906983; 13:21906983; 47:21906983; 107:21906983 RNF216 Homo sapiens (Human) 408; 822; 157; 419; 542; 723; 830; 641; 448; 482 "408,ASQDETKLPKIDF; 822,NGENTFKRIGPPL; 157,QTEREPKPGPSHN; 419,DFFDYSKLTPLDQ; 542,QEFYEQKIKEMAE; 723,RTSIEEKMTAARI; 830,IGPPLEKPVEKVQ; 641,PQTILYKYYERKA; 448,LSSQDIKWALHEL; 482,SPETSGKRKKRKQ" "408:21906983; 822:21906983; 157:21906983; 419:21906983; 542:21906983; 723:21906983; 830:21906983; 641:21890473; 448:21890473,21906983; 482:21906983" RNF216 Homo sapiens (Human) 425; 100; 773; 666; 765; 351; 485; 584; 391; 362 "425,SPETSGKRKKRKQ; 100,QTEREPKPGPSHN; 773,IGPPLEKPVEKVQ; 666,RTSIEEKMTAARI; 765,NGENTFKRIGPPL; 351,ASQDETKLPKIDF; 485,QEFYEQKIKEMAE; 584,PQTILYKYYERKA; 391,LSSQDIKWALHEL; 362,DFFDYSKLTPLDQ" "425:21906983; 100:21906983; 773:21906983; 666:21906983; 765:21906983; 351:21906983; 485:21906983; 584:21890473; 391:21890473,21906983; 362:21906983"