PTM - CPLM Gene Name UniProt ID Type Position Code NLE1; HUSSY-07 Q9NVX2 Ubiquitination 326 K NLE1; HUSSY-07 Q9NVX2 Ubiquitination 447 K NLE1; HUSSY-07 Q9NVX2 Ubiquitination 176 K NLE1; HUSSY-07 Q9NVX2 Ubiquitination 439 K NLE1; HUSSY-07 Q9NVX2 Ubiquitination 189 K NLE1; HUSSY-07 Q9NVX2 Ubiquitination 224 K

PTM - dbPAF Gene Name UniProt ID Position Type Species NLE1; HUSSY-07 Q9NVX2 128 Y Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 131 S Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 133 S Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 136 T Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 137 T Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 161 S Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 246 T Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 248 S Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 381 S Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 384 S Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 40 T Homo sapiens(Human) NLE1; HUSSY-07 Q9NVX2 79 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9NVX2 T40-p Phosphorylation FDVPVDItPDRLQLV Q9NVX2 S79-p Phosphorylation SLGKTLEsQAVETEK Q9NVX2 Y128-p Phosphorylation AFSPTGKyLASGSGD Q9NVX2 K224-ub Ubiquitination RYVASSSkDGSVRIW Q9NVX2 K326-ub Ubiquitination QGSLQELkERALSRY

PTM - dbPTM UniProt ID Position Code Type Resources References Q9NVX2 2 A Acetylation Swiss-Prot 1010711 19413330 Q9NVX2 79 S Phosphorylation Swiss-Prot 1010711 17525332 Q9NVX2 79 S Phosphorylation PhosphoELM.10011 17525332 Q9NVX2 224 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NVX2 326 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9NVX2 79 S Phosphorylation Phosphositeplus.1010730 17525332 Q9NVX2 40 T Phosphorylation Phosphositeplus.1010730 20068231 Q9NVX2 128 Y Phosphorylation Phosphositeplus.1010730 20068231 Q9NVX2 79 S Phosphorylation HPRD 9.0 17525332 Q9NVX2 131 S Phosphorylation HPRD 9.0 20068231 Q9NVX2 133 S Phosphorylation HPRD 9.0 20068231 Q9NVX2 381 S Phosphorylation HPRD 9.0 20068231 Q9NVX2 384 S Phosphorylation HPRD 9.0 20068231 Q9NVX2 40 T Phosphorylation HPRD 9.0 20068231 Q9NVX2 136 T Phosphorylation HPRD 9.0 20068231 Q9NVX2 137 T Phosphorylation HPRD 9.0 20068231 Q9NVX2 246 T Phosphorylation HPRD 9.0 20068231 Q9NVX2 128 Y Phosphorylation HPRD 9.0 20068231

PTM - HPRD Symbol Position Code Type References NLE1 128 Y Phosphorylation 20068231 NLE1 131 S Phosphorylation 20068231 NLE1 133 S Phosphorylation 20068231 NLE1 136 T Phosphorylation 20068231 NLE1 137 T Phosphorylation 20068231 NLE1 246 T Phosphorylation 20068231 NLE1 381 S Phosphorylation 20068231 NLE1 384 S Phosphorylation 20068231 NLE1 40 T Phosphorylation 20068231 NLE1 79 S Phosphorylation 17525332 NLE1 89 S Phosphorylation 20068231 NLE1 92 S Phosphorylation 20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9NVX2 79 S 17525332 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9NVX2 2 N-acetylalanine 19413330;22223895;22814378 Q9NVX2 79 Phosphoserine 17525332

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type NLE1 IPI00018196 S 130 TGKYLApSGSGDTT phosphorylation NLE1 IPI00018196 S 380 INQVLFpSPDSRIV phosphorylation NLE1 IPI00018196 S 383 VLFSPDpSRIVASA phosphorylation NLE1 IPI00018196 T 135 ASGSGDpTTVRFWD phosphorylation NLE1 IPI00018196 T 136 SGSGDTpTVRFWDL phosphorylation NLE1 IPI00018196 T 39 DVPVDIpTPDRLQL phosphorylation NLE1 IPI00018196 Y 127 FSPTGKpYLASGSG phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 54475 119980 - K Ubiquitination 21906983 54475 119980 - K Ubiquitination 22505724 54475 119980 - K Ubiquitination 21963094 54475 119980 - K Neddylation 24654937 54475 119980 - K Sumoylation 24654937 54475 119980 - K Ubiquitination 24816145

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID NLE1 Homo sapiens (Human) 302 "302,GSLQELKERALSR" 302:21906983 NLE1 Homo sapiens (Human) 182; 284 "182,YVASSSKDGSVRI; 284,GSLQELKERALSR" 182:21906983; 284:21906983 NLE1 Homo sapiens (Human) 326; 224 "326,GSLQELKERALSR; 224,YVASSSKDGSVRI" 326:21906983; 224:21906983