PTM - CPLM Gene Name UniProt ID Type Position Code ITCH Q96J02 Ubiquitination 446 K ITCH Q96J02 Ubiquitination 432 K ITCH Q96J02 Ubiquitination 478 K ITCH Q96J02 Ubiquitination 34 K ITCH Q96J02 Ubiquitination 66 K ITCH Q96J02 Ubiquitination 513 K ITCH Q96J02 Ubiquitination 861 K ITCH Q96J02 Ubiquitination 881 K ITCH Q96J02 Ubiquitination 350 K ITCH Q96J02 Ubiquitination 858 K

PTM - dbPAF Gene Name UniProt ID Position Type Species ITCH Q96J02 10 S Homo sapiens(Human) ITCH Q96J02 13 S Homo sapiens(Human) ITCH Q96J02 15 T Homo sapiens(Human) ITCH Q96J02 2 S Homo sapiens(Human) ITCH Q96J02 214 T Homo sapiens(Human) ITCH Q96J02 217 S Homo sapiens(Human) ITCH Q96J02 218 T Homo sapiens(Human) ITCH Q96J02 221 S Homo sapiens(Human) ITCH Q96J02 236 S Homo sapiens(Human) ITCH Q96J02 240 S Homo sapiens(Human) ITCH Q96J02 242 S Homo sapiens(Human) ITCH Q96J02 244 S Homo sapiens(Human) ITCH Q96J02 251 S Homo sapiens(Human) ITCH Q96J02 343 Y Homo sapiens(Human) ITCH Q96J02 344 Y Homo sapiens(Human) ITCH Q96J02 375 Y Homo sapiens(Human) ITCH Q96J02 385 T Homo sapiens(Human) ITCH Q96J02 393 S Homo sapiens(Human) ITCH Q96J02 397 Y Homo sapiens(Human) ITCH Q96J02 4 S Homo sapiens(Human) ITCH Q96J02 420 Y Homo sapiens(Human) ITCH Q96J02 450 S Homo sapiens(Human) ITCH Q96J02 495 Y Homo sapiens(Human) ITCH Q96J02 503 T Homo sapiens(Human) ITCH Q96J02 504 T Homo sapiens(Human) ITCH Q96J02 505 T Homo sapiens(Human) ITCH Q96J02 506 Y Homo sapiens(Human) ITCH Q96J02 589 Y Homo sapiens(Human) ITCH Q96J02 6 S Homo sapiens(Human) ITCH Q96J02 70 T Homo sapiens(Human) ITCH Q96J02 74 T Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q96J02 K53-m1 Methylation TVDGQSKkTEKCNNT Q96J02 S2-p Phosphorylation ______MsDSGsQLG Q96J02 S6-p Phosphorylation __MsDSGsQLGsMGS Q96J02 S10-p Phosphorylation DSGsQLGsMGSLTMK Q96J02 T214-p Phosphorylation GSRSKDEtRVstNGs Q96J02 S217-p Phosphorylation SKDEtRVstNGsDDP Q96J02 T218-p Phosphorylation KDEtRVstNGsDDPE Q96J02 S221-p Phosphorylation tRVstNGsDDPEDAG Q96J02 S236-p Phosphorylation AGENRRVsGNNsPSL Q96J02 S240-p Phosphorylation RRVsGNNsPSLsNGG Q96J02 S244-p Phosphorylation GNNsPSLsNGGFKPs Q96J02 S251-p Phosphorylation sNGGFKPsRPPRPsR Q96J02 S257-p Phosphorylation PsRPPRPsRPPPPTP Q96J02 Y343-p Phosphorylation VDQHGRVyyVDHVEk Q96J02 Y344-p Phosphorylation DQHGRVyyVDHVEkR Q96J02 Y375-p Phosphorylation VDNMGRIyYVDHFTR Q96J02 T385-p Phosphorylation DHFTRTTtWQRPTLE Q96J02 S393-p Phosphorylation WQRPTLEsVRNyEQW Q96J02 Y397-p Phosphorylation TLEsVRNyEQWQLQR Q96J02 Y420-p Phosphorylation QFNQRFIyGNQDLFA Q96J02 S450-p Phosphorylation GWEkRTDsNGRVYFV Q96J02 Y495-p Phosphorylation FTVDGIPyFVDHNRR Q96J02 T503-p Phosphorylation FVDHNRRtttyIDPR Q96J02 T504-p Phosphorylation VDHNRRtttyIDPRT Q96J02 T505-p Phosphorylation DHNRRtttyIDPRTG Q96J02 Y506-p Phosphorylation HNRRtttyIDPRTGk Q96J02 Y589-p Phosphorylation PGEEGLDyGGVAREW Q96J02 K34-ub Ubiquitination AKLKENKkNWFGPSP Q96J02 K66-ub Ubiquitination NTNSPKWkQPLTVIV Q96J02 K90-ub Ubiquitination VWSHQTLkSDVLLGT Q96J02 K107-ub Ubiquitination LDIYETLkSNNMKLE Q96J02 K350-ub Ubiquitination yyVDHVEkRTTWDRP Q96J02 K432-ub Ubiquitination LFATSQSkEFDPLGP Q96J02 K446-ub Ubiquitination PLPPGWEkRTDsNGR Q96J02 K478-ub Ubiquitination SQGQLNEkPLPEGWE Q96J02 K513-ub Ubiquitination yIDPRTGkSALDNGP Q96J02 K665-ub Ubiquitination GFSLPFYkRILNKPV Q96J02 K861-ub Ubiquitination FCIEKVGkENWLPRS Q96J02 K881-ub Ubiquitination RLDLPPYkSYEQLKE

PTM - dbPTM UniProt ID Position Code Type Resources References Q96J02 385 T Phosphorylation Swiss-Prot 1010711 16888620 Q96J02 420 Y Phosphorylation Swiss-Prot 1010711 16387660;17053785 Q96J02 450 S Phosphorylation Swiss-Prot 1010711 16888620 Q96J02 420 Y Phosphorylation PhosphoELM.10011 16387660 Q96J02 34 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q96J02 432 K Ubiquitylation Phosphositeplus.1010730 20972266;21890473;21906983 Q96J02 446 K Ubiquitylation Phosphositeplus.1010730 20639865;21890473;21906983 Q96J02 478 K Ubiquitylation Phosphositeplus.1010730 20972266;21890473;21906983 Q96J02 513 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q96J02 66 K Ubiquitylation Phosphositeplus.1010730 21890473 Q96J02 861 K Ubiquitylation Phosphositeplus.1010730 20972266;21890473 Q96J02 343 Y Phosphorylation Phosphositeplus.1010730 19534553 Q96J02 420 Y Phosphorylation Phosphositeplus.1010730 16387660;17053785 Q96J02 2 S Acetylation HPRD 9.0 19413330 Q96J02 2 S Phosphorylation HPRD 9.0 20068231 Q96J02 4 S Phosphorylation HPRD 9.0 20068231 Q96J02 10 S Phosphorylation HPRD 9.0 20068231 Q96J02 13 S Phosphorylation HPRD 9.0 20068231 Q96J02 15 T Phosphorylation HPRD 9.0 20068231

PTM - HPRD Symbol Position Code Type References ITCH 10 S Phosphorylation 20068231 ITCH 13 S Phosphorylation 20068231 ITCH 15 T Phosphorylation 20068231 ITCH 2 S Acetylation 19413330 ITCH 2 S Phosphorylation 20068231 ITCH 4 S Phosphorylation 20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q96J02 420 Y 16387660 Fyn LTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q96J02 2 N-acetylserine 19413330 Q96J02 385 Phosphothreonine; by SGK3 16888620 Q96J02 420 Phosphotyrosine; by FYN 16387660 Q96J02 450 Phosphoserine; by SGK3 16888620

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type ITCH IPI00061780 S 13 QLGSMGpSLTMKSQ phosphorylation ITCH IPI00061780 T 15 GSMGSLpTMKSQLQ phosphorylation ITCH IPI00061780 T 70 KWKQPLpTVIVTPV phosphorylation ITCH IPI00061780 T 74 PLTVIVpTPVSKLH phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 83737 123747 - K Ubiquitination 17038327 83737 123747 - K Ubiquitination 17013377 83737 123747 - K Ubiquitination 20484045 83737 123747 - K Ubiquitination 19341794 83737 123747 - K Ubiquitination 19343052 83737 123747 - K Ubiquitination 20818436 83737 123747 - K Ubiquitination 19808964 83737 123747 - K Ubiquitination 20504295 83737 123747 - K Ubiquitination 20639865 83737 123747 - K Ubiquitination 21987572 83737 123747 - K Ubiquitination 20972266 83737 123747 - K Ubiquitination 21139048 83737 123747 - K Ubiquitination 21890473 83737 123747 - K Ubiquitination 21906983 83737 123747 - K Ubiquitination 22053931 83737 123747 - K Ubiquitination 22505724 83737 123747 - K Ubiquitination 21963094 83737 123747 - K Ubiquitination 23000965 83737 123747 - K Ubiquitination 23564455 83737 123747 - K Ubiquitination 24101513 83737 123747 - K Ubiquitination 25147182

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID ITCH Homo sapiens (Human) 34; 343; 357; 424; 820; 472; 66; 840; 391; 437; 389; 405 "34,KLKENKKNWFGPS; 343,FATSQSKEFDPLG; 357,LPPGWEKRTDSNG; 424,IDPRTGKSALDNG; 820,CIEKVGKENWLPR; 472,IDPRTGKSALDNG; 66,TNSPKWKQPLTVI; 840,LDLPPYKSYEQLK; 391,FATSQSKEFDPLG; 437,QGQLNEKPLPEGW; 389,QGQLNEKPLPEGW; 405,LPPGWEKRTDSNG" "34:21906983,21890473; 343:21906983; 357:21906983; 424:21906983; 820:21890473; 472:21890473; 66:21890473; 840:21890473; 391:21890473; 437:21890473; 389:21906983; 405:21890473" ITCH Homo sapiens (Human) 34; 820; 66; 472; 840; 391; 437; 405 "34,KLKENKKNWFGPS; 820,CIEKVGKENWLPR; 66,TNSPKWKQPLTVI; 472,IDPRTGKSALDNG; 840,LDLPPYKSYEQLK; 391,FATSQSKEFDPLG; 437,QGQLNEKPLPEGW; 405,LPPGWEKRTDSNG" 34:21890473; 820:21890473; 66:21890473; 472:21890473; 840:21890473; 391:21890473; 437:21890473; 405:21890473 ITCH Homo sapiens (Human) 820; 391; 437 "820,CIEKVGKENWLPR; 391,FATSQSKEFDPLG; 437,QGQLNEKPLPEGW" 820:20972266; 391:20972266; 437:20972266 ITCH Homo sapiens (Human) 34; 820; 66; 472; 840; 391; 437; 405 "34,KLKENKKNWFGPS; 820,CIEKVGKENWLPR; 66,TNSPKWKQPLTVI; 472,IDPRTGKSALDNG; 840,LDLPPYKSYEQLK; 391,FATSQSKEFDPLG; 437,QGQLNEKPLPEGW; 405,LPPGWEKRTDSNG" 34:21890473; 820:21890473; 66:21890473; 472:21890473; 840:21890473; 391:21890473; 437:21890473; 405:21890473 ITCH Homo sapiens (Human) 478; 446; 432; 350 "478,QGQLNEKPLPEGW; 446,LPPGWEKRTDSNG; 432,FATSQSKEFDPLG; 350,YVDHVEKRTTWDR" "478:21139048; 446:21139048,22053931; 432:21139048,22053931; 350:22053931" ITCH Homo sapiens (Human) 34; 478; 881; 513; 66; 446; 432; 350; 861 "34,KLKENKKNWFGPS; 478,QGQLNEKPLPEGW; 881,LDLPPYKSYEQLK; 513,IDPRTGKSALDNG; 66,TNSPKWKQPLTVI; 446,LPPGWEKRTDSNG; 432,FATSQSKEFDPLG; 350,YVDHVEKRTTWDR; 861,CIEKVGKENWLPR" "34:21890473,21906983; 478:21890473,21906983; 881:21890473; 513:21890473,21906983; 66:21890473; 446:21890473,21906983,22053931; 432:21890473,21906983,22053931; 350:22053931; 861:21890473" ITCH Homo sapiens (Human) 34; 820; 66; 472; 840; 391; 437; 405 "34,KLKENKKNWFGPS; 820,CIEKVGKENWLPR; 66,TNSPKWKQPLTVI; 472,IDPRTGKSALDNG; 840,LDLPPYKSYEQLK; 391,FATSQSKEFDPLG; 437,QGQLNEKPLPEGW; 405,LPPGWEKRTDSNG" "34:21890473,21906983; 820:21890473; 66:21890473; 472:21890473,21906983; 840:21890473; 391:21890473,21906983; 437:21890473,21906983; 405:21890473,21906983" ITCH Homo sapiens (Human) 34; 820; 66; 472; 840; 391; 437; 405 "34,KLKENKKNWFGPS; 820,CIEKVGKENWLPR; 66,TNSPKWKQPLTVI; 472,IDPRTGKSALDNG; 840,LDLPPYKSYEQLK; 391,FATSQSKEFDPLG; 437,QGQLNEKPLPEGW; 405,LPPGWEKRTDSNG" 34:21890473; 820:21890473; 66:21890473; 472:21890473; 840:21890473; 391:21890473; 437:21890473; 405:21890473