PTM - CPLM Gene Name UniProt ID Type Position Code ANAPC5; APC5 Q9UJX4 Ubiquitination 229 K ANAPC5; APC5 Q9UJX4 Ubiquitination 238 K ANAPC5; APC5 Q9UJX4 Ubiquitination 289 K ANAPC5; APC5 Q9UJX4 Ubiquitination 403 K ANAPC5; APC5 Q9UJX4 Ubiquitination 562 K ANAPC5; APC5 Q9UJX4 Ubiquitination 698 K ANAPC5; APC5 Q9UJX4 Ubiquitination 547 K ANAPC5; APC5 Q9UJX4 Ubiquitination 669 K ANAPC5; APC5 Q9UJX4 Ubiquitination 524 K ANAPC5; APC5 Q9UJX4 Ubiquitination 682 K ANAPC5; APC5 Q9UJX4 Ubiquitination 210 K ANAPC5; APC5 Q9UJX4 Ubiquitination 245 K ANAPC5; APC5 Q9UJX4 Ubiquitination 683 K ANAPC5; APC5 Q9UJX4 Ubiquitination 80 K ANAPC5; APC5 Q9UJX4 Ubiquitination 726 K ANAPC5; APC5 Q9UJX4 Ubiquitination 703 K ANAPC5; APC5 Q9UJX4 Ubiquitination 224 K ANAPC5; APC5 Q9UJX4 Ubiquitination 367 K ANAPC5; APC5 Q9UJX4 Ubiquitination 397 K ANAPC5; APC5 Q9UJX4 Ubiquitination 167 K ANAPC5; APC5 Q9UJX4 Ubiquitination 168 K ANAPC5; APC5 Q9UJX4 Ubiquitination 366 K ANAPC5; APC5 Q9UJX4 Ubiquitination 393 K

PTM - dbPAF Gene Name UniProt ID Position Type Species ANAPC5; APC5 Q9UJX4 15 T Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 178 T Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 179 S Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 195 S Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 202 S Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 204 S Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 217 S Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 221 S Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 232 T Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 235 S Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 255 Y Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 258 Y Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 277 Y Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 413 S Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 421 S Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 47 T Homo sapiens(Human) ANAPC5; APC5 Q9UJX4 674 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9UJX4 T15-p Phosphorylation LYFNPMMtNGVVHAN Q9UJX4 T47-p Phosphorylation LLNEMSRtGEGAVSL Q9UJX4 T178-p Phosphorylation EDADMELtsRDEGER Q9UJX4 S179-p Phosphorylation DADMELtsRDEGERK Q9UJX4 S195-p Phosphorylation EKEELDVsVREEEVS Q9UJX4 S217-p Phosphorylation KQAEFFLsQQAsLLk Q9UJX4 S221-p Phosphorylation FFLsQQAsLLkNDET Q9UJX4 T232-p Phosphorylation NDETkALtPAsLQkE Q9UJX4 S235-p Phosphorylation TkALtPAsLQkELNN Q9UJX4 Y255-p Phosphorylation PDFAEAHyLSyLNNL Q9UJX4 Y258-p Phosphorylation AEAHyLSyLNNLRVQ Q9UJX4 Y277-p Phosphorylation STHSLLHyFDRLILT Q9UJX4 S421-p Phosphorylation LSELIDIsIAQKTAI Q9UJX4 S596-p Phosphorylation ELYWRSSsPtIALPM Q9UJX4 T598-p Phosphorylation YWRSSsPtIALPMLL Q9UJX4 S674-p Phosphorylation VAKCQVAsAASYDQP Q9UJX4 K77-ub Ubiquitination GPDITLSkLYKLIEE Q9UJX4 K167-ub Ubiquitination QYFQNGEkkTVEDAD Q9UJX4 K168-ub Ubiquitination YFQNGEkkTVEDADM Q9UJX4 K224-ub Ubiquitination sQQAsLLkNDETkAL Q9UJX4 K229-ub Ubiquitination LLkNDETkALtPAsL Q9UJX4 K238-ub Ubiquitination LtPAsLQkELNNLLk Q9UJX4 K245-ub Ubiquitination kELNNLLkFNPDFAE Q9UJX4 K289-ub Ubiquitination ILTGAESkSNGEEGY Q9UJX4 K366-ub Ubiquitination VLLEHSVkKAVHFGL Q9UJX4 K393-ub Ubiquitination QQRAFAGkTANkLMD Q9UJX4 K397-ub Ubiquitination FAGkTANkLMDALkD Q9UJX4 K403-ub Ubiquitination NkLMDALkDSDLLHW Q9UJX4 K524-ub Ubiquitination DRAMNDGkYHLADSL Q9UJX4 K547-ub Ubiquitination SIEGVYRkAVVLQAQ Q9UJX4 K562-ub Ubiquitination NQMSEAHkLLQKLLV Q9UJX4 K683-ub Ubiquitination ASYDQPKkAEALEAA Q9UJX4 K698-ub Ubiquitination IENLNEAkNYFAkVD Q9UJX4 K703-ub Ubiquitination EAkNYFAkVDCKERI Q9UJX4 K726-ub Ubiquitination RLYHTLGkTQERNRC

PTM - dbPTM UniProt ID Position Code Type Resources References Q9UJX4 178 T Phosphorylation Swiss-Prot 1010711 19007248 Q9UJX4 195 S Phosphorylation Swiss-Prot 1010711 14657031 Q9UJX4 217 S Phosphorylation Swiss-Prot 1010711 15302935 Q9UJX4 179 S Phosphorylation PhosphoELM.10011 22067460 Q9UJX4 195 S Phosphorylation PhosphoELM.10011 22067460;14657031 Q9UJX4 202 S Phosphorylation PhosphoELM.10011 15302935 Q9UJX4 217 S Phosphorylation PhosphoELM.10011 15302935 Q9UJX4 221 S Phosphorylation PhosphoELM.10011 22067460 Q9UJX4 178 T Phosphorylation PhosphoELM.10011 22067460 Q9UJX4 232 T Phosphorylation PhosphoELM.10011 22067460 Q9UJX4 167 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 168 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 224 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 229 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UJX4 238 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UJX4 245 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 289 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UJX4 366 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 393 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 397 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 403 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UJX4 524 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 547 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 562 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 683 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 698 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 726 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX4 179 S Phosphorylation Phosphositeplus.1010730 22067460 Q9UJX4 195 S Phosphorylation Phosphositeplus.1010730 14657031;18420821;21406692;22067460 Q9UJX4 217 S Phosphorylation Phosphositeplus.1010730 15302935;19690332 Q9UJX4 221 S Phosphorylation Phosphositeplus.1010730 18420821;22067460 Q9UJX4 421 S Phosphorylation Phosphositeplus.1010730 20068231 Q9UJX4 674 S Phosphorylation Phosphositeplus.1010730 18420821 Q9UJX4 15 T Phosphorylation Phosphositeplus.1010730 18420821 Q9UJX4 178 T Phosphorylation Phosphositeplus.1010730 22067460 Q9UJX4 232 T Phosphorylation Phosphositeplus.1010730 18420821;22067460 Q9UJX4 217 S Phosphorylation HPRD 9.0 15302935 Q9UJX4 413 S Phosphorylation HPRD 9.0 20068231 Q9UJX4 421 S Phosphorylation HPRD 9.0 20068231 Q9UJX4 178 T Phosphorylation HPRD 9.0 19007248

PTM - HPRD Symbol Position Code Type References ANAPC5 178 T Phosphorylation 19007248 ANAPC5 217 S Phosphorylation 15302935 ANAPC5 413 S Phosphorylation 20068231 ANAPC5 421 S Phosphorylation 20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9UJX4 178 T 22067460 HTP Phosphorylation Q9UJX4 202 S 15302935 HTP Phosphorylation Q9UJX4 232 T 22067460 HTP Phosphorylation Q9UJX4 195 S 22067460 HTP Phosphorylation Q9UJX4 195 S 14657031 HTP Phosphorylation Q9UJX4 221 S 22067460 HTP Phosphorylation Q9UJX4 217 S 15302935 HTP Phosphorylation Q9UJX4 179 S 22067460 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9UJX4 195 Phosphoserine 14657031 Q9UJX4 232 Phosphothreonine 23186163

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 51433 119537 - K Ubiquitination 21987572 51433 119537 - K Ubiquitination 21139048 51433 119537 - K Ubiquitination 21890473 51433 119537 - K Ubiquitination 21906983 51433 119537 - K Ubiquitination 22053931 51433 119537 - K Ubiquitination 22505724 51433 119537 - K Ubiquitination 21963094 51433 119537 - K Ubiquitination 25015289 51433 119537 - K Ubiquitination 25147182 51433 119537 - K Neddylation 18247557

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID ANAPC5 Homo sapiens (Human) 262; 247; 126 "262,ENLNEAKNYFAKV; 247,SYDQPKKAEALEA; 126,QMSEAHKLLQKLL" 262:21906983; 247:21906983; 126:21890473 ANAPC5 Homo sapiens (Human) 139; 291; 190; 126; 130; 450 "139,TPASLQKELNNLL; 291,KLMDALKDSDLLH; 190,LTGAESKSNGEEG; 126,RAMNDGKYHLADS; 130,LKNDETKALTPAS; 450,QMSEAHKLLQKLL" 139:21890473; 291:21890473; 190:21890473; 126:21906983; 130:21890473; 450:21890473 ANAPC5 Homo sapiens (Human) 392; 190; 364; 213; 349; 228 "392,LYHTLGKTQERNR; 190,RAMNDGKYHLADS; 364,ENLNEAKNYFAKV; 213,IEGVYRKAVVLQA; 349,SYDQPKKAEALEA; 228,QMSEAHKLLQKLL" 392:21906983; 190:21906983; 364:21906983; 213:21906983; 349:21906983; 228:21906983 ANAPC5 Homo sapiens (Human) 713; 238; 685; 549; 168; 511; 380; 229; 167; 224; 289; 670; 534; 366; 384; 245; 390 "713,LYHTLGKTQERNR; 238,TPASLQKELNNLL; 685,ENLNEAKNYFAKV; 549,QMSEAHKLLQKLL; 168,FQNGEKKTVEDAD; 511,RAMNDGKYHLADS; 380,PRAFAGKTANKLM; 229,LKNDETKALTPAS; 167,YFQNGEKKTVEDA; 224,QQASLLKNDETKA; 289,LTGAESKSNGEEG; 670,SYDQPKKAEALEA; 534,IEGVYRKAVVLQA; 366,LLEHSVKKAVHFG; 384,AGKTANKLMDALK; 245,ELNNLLKFNPDFA; 390,KLMDALKDSDLLH" 713:21906983; 238:21906983; 685:21906983; 549:21906983; 168:21906983; 511:21906983; 380:21906983; 229:21906983; 167:21906983; 224:21906983; 289:21906983; 670:21906983; 534:21906983; 366:21906983; 384:21906983; 245:21906983; 390:21906983 ANAPC5 Homo sapiens (Human) 238; 289; 562; 403; 229 "238,TPASLQKELNNLL; 289,LTGAESKSNGEEG; 562,QMSEAHKLLQKLL; 403,KLMDALKDSDLLH; 229,LKNDETKALTPAS" 238:21890473; 289:21890473; 562:21890473; 403:21890473; 229:21890473 ANAPC5 Homo sapiens (Human) 238; 168; 683; 547; 524; 403; 229; 167; 224; 289; 393; 698; 562; 397; 366; 245; 726 "238,TPASLQKELNNLL; 168,FQNGEKKTVEDAD; 683,SYDQPKKAEALEA; 547,IEGVYRKAVVLQA; 524,RAMNDGKYHLADS; 403,KLMDALKDSDLLH; 229,LKNDETKALTPAS; 167,YFQNGEKKTVEDA; 224,QQASLLKNDETKA; 289,LTGAESKSNGEEG; 393,QRAFAGKTANKLM; 698,ENLNEAKNYFAKV; 562,QMSEAHKLLQKLL; 397,AGKTANKLMDALK; 366,LLEHSVKKAVHFG; 245,ELNNLLKFNPDFA; 726,LYHTLGKTQERNR" "238:21890473,21906983; 168:21906983; 683:21906983; 547:21906983; 524:21906983; 403:21890473,21906983; 229:21890473,21906983; 167:21906983; 224:21906983; 289:21890473,21906983,22053931; 393:21906983; 698:21906983; 562:21890473,21906983; 397:21906983; 366:21906983; 245:21906983; 726:21906983" ANAPC5 Homo sapiens (Human) 103; 117; 168; 108; 124; 46; 47 "103,QQASLLKNDETKA; 117,TPASLQKELNNLL; 168,LTGAESKSNGEEG; 108,LKNDETKALTPAS; 124,ELNNLLKFNPDFA; 46,YFQNGEKKTVEDA; 47,FQNGEKKTVEDAD" "103:21906983; 117:21890473,21906983; 168:21890473,21906983; 108:21890473,21906983; 124:21906983; 46:21906983; 47:21906983" ANAPC5 Homo sapiens (Human) 289 "289,LTGAESKSNGEEG" 289:22053931