PTM - CPLM Gene Name UniProt ID Type Position Code USP48; USP31 Q86UV5 Acetylation 956 K USP48; USP31 Q86UV5 Ubiquitination 170 K USP48; USP31 Q86UV5 Ubiquitination 587 K USP48; USP31 Q86UV5 Ubiquitination 681 K USP48; USP31 Q86UV5 Ubiquitination 868 K USP48; USP31 Q86UV5 Ubiquitination 945 K USP48; USP31 Q86UV5 Ubiquitination 956 K USP48; USP31 Q86UV5 Ubiquitination 199 K USP48; USP31 Q86UV5 Ubiquitination 368 K

PTM - dbPAF Gene Name UniProt ID Position Type Species USP48; USP31 Q86UV5 162 Y Homo sapiens(Human) USP48; USP31 Q86UV5 287 S Homo sapiens(Human) USP48; USP31 Q86UV5 326 Y Homo sapiens(Human) USP48; USP31 Q86UV5 394 S Homo sapiens(Human) USP48; USP31 Q86UV5 398 T Homo sapiens(Human) USP48; USP31 Q86UV5 501 S Homo sapiens(Human) USP48; USP31 Q86UV5 502 T Homo sapiens(Human) USP48; USP31 Q86UV5 620 S Homo sapiens(Human) USP48; USP31 Q86UV5 627 S Homo sapiens(Human) USP48; USP31 Q86UV5 628 T Homo sapiens(Human) USP48; USP31 Q86UV5 820 T Homo sapiens(Human) USP48; USP31 Q86UV5 859 Y Homo sapiens(Human) USP48; USP31 Q86UV5 865 Y Homo sapiens(Human) USP48; USP31 Q86UV5 886 S Homo sapiens(Human) USP48; USP31 Q86UV5 887 S Homo sapiens(Human) USP48; USP31 Q86UV5 888 S Homo sapiens(Human) USP48; USP31 Q86UV5 890 T Homo sapiens(Human) USP48; USP31 Q86UV5 910 S Homo sapiens(Human) USP48; USP31 Q86UV5 914 T Homo sapiens(Human) USP48; USP31 Q86UV5 924 Y Homo sapiens(Human) USP48; USP31 Q86UV5 935 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q86UV5 K956-ac Acetylation VSANQTLkELKIQIM Q86UV5 R267-m1 Methylation EKLEGDNrYFCENCQ Q86UV5 Y162-p Phosphorylation LQNSNRRyIDPSGFV Q86UV5 Y326-p Phosphorylation EILDMEPyVEHKGGS Q86UV5 S394-p Phosphorylation EEDLAEPsKSQtRKP Q86UV5 T398-p Phosphorylation AEPsKSQtRKPKCGK Q86UV5 S620-p Phosphorylation QDGDAEQsNGKMNGs Q86UV5 S627-p Phosphorylation sNGKMNGstLNKDES Q86UV5 T628-p Phosphorylation NGKMNGstLNKDESK Q86UV5 T820-p Phosphorylation VDHVIKItRIEVGDV Q86UV5 Y859-p Phosphorylation QQRDLREyTQATIyV Q86UV5 Y865-p Phosphorylation EyTQATIyVHkVVDN Q86UV5 S886-p Phosphorylation SAPELNVsssETEED Q86UV5 S887-p Phosphorylation APELNVsssETEEDK Q86UV5 S888-p Phosphorylation PELNVsssETEEDKE Q86UV5 S910-p Phosphorylation KDPDFNQsNGGTKRQ Q86UV5 Y924-p Phosphorylation QKISHQNyIAYQkQV Q86UV5 S935-p Phosphorylation QkQVIRRsMRHRKVR Q86UV5 K9-ub Ubiquitination APRLQLEkAAWRWAE Q86UV5 K170-ub Ubiquitination IDPSGFVkALGLDTG Q86UV5 K199-ub Ubiquitination LLEDTLSkQKNPDVR Q86UV5 K258-ub Ubiquitination DCISEFLkEEKLEGD Q86UV5 K276-ub Ubiquitination FCENCQSkQNATRKI Q86UV5 K368-ub Ubiquitination PQSGEWYkFNDEDIE Q86UV5 K551-ub Ubiquitination GGPRLTVkALCKECV Q86UV5 K587-ub Ubiquitination NLLKAAVkGSDGFWV Q86UV5 K669-ub Ubiquitination NERRLVSkEAWSKLQ Q86UV5 K681-ub Ubiquitination KLQQYFPkAPEFPSY Q86UV5 K689-ub Ubiquitination APEFPSYkECCSQCk Q86UV5 K696-ub Ubiquitination kECCSQCkILEREGE Q86UV5 K868-ub Ubiquitination QATIyVHkVVDNKKV Q86UV5 K929-ub Ubiquitination QNyIAYQkQVIRRsM Q86UV5 K945-ub Ubiquitination HRKVRGEkALLVSAN Q86UV5 K956-ub Ubiquitination VSANQTLkELKIQIM

PTM - dbPTM UniProt ID Position Code Type Resources References Q86UV5 886 S Phosphorylation Swiss-Prot 1010711 17081983;19413330 Q86UV5 887 S Phosphorylation Swiss-Prot 1010711 17081983 Q86UV5 888 S Phosphorylation Swiss-Prot 1010711 17081983 Q86UV5 890 T Phosphorylation Swiss-Prot 1010711 17081983 Q86UV5 956 K Acetylation Swiss-Prot 1010711 19608861 Q86UV5 170 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q86UV5 199 K Ubiquitylation Phosphositeplus.1010730 21906983 Q86UV5 368 K Ubiquitylation Phosphositeplus.1010730 21906983 Q86UV5 587 K Ubiquitylation Phosphositeplus.1010730 21890473 Q86UV5 681 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q86UV5 868 K Ubiquitylation Phosphositeplus.1010730 21890473 Q86UV5 945 K Ubiquitylation Phosphositeplus.1010730 21890473 Q86UV5 956 K Acetylation Phosphositeplus.1010730 19608861 Q86UV5 886 S Phosphorylation Phosphositeplus.1010730 20068231;21406692 Q86UV5 887 S Phosphorylation Phosphositeplus.1010730 20068231;21406692 Q86UV5 888 S Phosphorylation Phosphositeplus.1010730 20068231;20363803;21406692 Q86UV5 956 K Acetylation HPRD 9.0 19608861 Q86UV5 886 S Phosphorylation HPRD 9.0 17081983,19413330,19651622,20068231 Q86UV5 887 S Phosphorylation HPRD 9.0 17081983,19651622,20068231 Q86UV5 888 S Phosphorylation HPRD 9.0 17081983,19651622,20068231 Q86UV5 890 T Phosphorylation HPRD 9.0 17081983 Q86UV5 886 S Phosphorylation SysPTM 1.1 17081983 Q86UV5 887 S Phosphorylation SysPTM 1.1 17081983 Q86UV5 888 S Phosphorylation SysPTM 1.1 17081983 Q86UV5 890 T Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References USP48 886 S Phosphorylation 17081983,19413330,19651622,20068231 USP48 887 S Phosphorylation 17081983,19651622,20068231 USP48 888 S Phosphorylation 17081983,19651622,20068231 USP48 890 T Phosphorylation 17081983 USP48 956 K Acetylation 19608861

PTM - UniProt UniProt ID Position Type PMIDs Q86UV5 886 Phosphoserine 20068231;21406692 Q86UV5 887 Phosphoserine 20068231 Q86UV5 888 Phosphoserine 20068231;21406692 Q86UV5 956 N6-acetyllysine 19608861

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type USP48 IPI00328815 K 956 SANQTLaKELKIQI acetylation USP48 IPI00328815 S 287 RKIRLLpSLPCTLN phosphorylation USP48 IPI00328815 S 886 APELNVpSSSETEE phosphorylation USP48 IPI00328815 S 887 PELNVSpSSETEED phosphorylation USP48 IPI00328815 S 888 ELNVSSpSETEEDK phosphorylation USP48 IPI00328815 T 890 NVSSSEpTEEDKEE phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 84196 123941 - K Ubiquitination 22118674 84196 123941 - K Ubiquitination 21987572 84196 123941 - K Ubiquitination 21963094 84196 123941 - K Ubiquitination 21139048 84196 123941 - K Ubiquitination 21890473 84196 123941 - K Ubiquitination 21906983 84196 123941 - K Ubiquitination 23000965

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID USP48 Homo sapiens (Human) 170; 199 "170,DPSGFVKALGLDT; 199,LEDTLSKQKNPDV" "170:21890473,21906983; 199:21906983" USP48 Homo sapiens (Human) 219; 406 "219,LQQYFPKAPEFPS; 406,ATIYVHKVVDNKK" "219:21890473,21906983; 406:21890473" USP48 Homo sapiens (Human) 70; 81 "70,RKVRGEKALLVSA; 81,SANQTLKELKIQI" 70:21890473; 81:21890473 USP48 Homo sapiens (Human) 170; 368; 199 "170,DPSGFVKALGLDT; 368,QSGEWYKFNDEDI; 199,LEDTLSKQKNPDV" "170:21890473,21906983; 368:21906983; 199:21906983" USP48 Homo sapiens (Human) 170; 880; 957; 968; 368; 199; 586; 693 "170,DPSGFVKALGLDT; 880,ATIYVHKVVDNKK; 957,RKVRGEKALLVSA; 968,SANQTLKELKIQI; 368,QSGEWYKFNDEDI; 199,LEDTLSKQKNPDV; 586,LLKAAVKGSDGFW; 693,LQQYFPKAPEFPS" "170:21890473,21906983; 880:21890473; 957:21890473; 968:21890473; 368:21906983; 199:21906983; 586:21890473; 693:21890473,21906983" USP48 Homo sapiens (Human) 170; 868; 368; 681; 199; 587 "170,DPSGFVKALGLDT; 868,ATIYVHKVVDNKK; 368,QSGEWYKFNDEDI; 681,LQQYFPKAPEFPS; 199,LEDTLSKQKNPDV; 587,LLKAAVKGSDGFW" "170:21890473,21906983; 868:21890473; 368:21906983; 681:21890473,21906983; 199:21906983; 587:21890473" USP48 Homo sapiens (Human) 170; 368; 681; 199; 587 "170,DPSGFVKALGLDT; 368,QSGEWYKFNDEDI; 681,LQQYFPKAPEFPS; 199,LEDTLSKQKNPDV; 587,LLKAAVKGSDGFW" "170:21890473,21906983; 368:21906983; 681:21890473,21906983; 199:21906983; 587:21890473" USP48 Homo sapiens (Human) 170; 880; 957; 968; 586; 693 "170,DPSGFVKALGLDT; 880,ATIYVHKVVDNKK; 957,RKVRGEKALLVSA; 968,SANQTLKELKIQI; 586,LLKAAVKGSDGFW; 693,LQQYFPKAPEFPS" 170:21890473; 880:21890473; 957:21890473; 968:21890473; 586:21890473; 693:21890473 USP48 Homo sapiens (Human) 170; 880; 957; 968; 586; 693 "170,DPSGFVKALGLDT; 880,ATIYVHKVVDNKK; 957,RKVRGEKALLVSA; 968,SANQTLKELKIQI; 586,LLKAAVKGSDGFW; 693,LQQYFPKAPEFPS" 170:21890473; 880:21890473; 957:21890473; 968:21890473; 586:21890473; 693:21890473 USP48 Homo sapiens (Human) 170; 868; 681; 587 "170,DPSGFVKALGLDT; 868,ATIYVHKVVDNKK; 681,LQQYFPKAPEFPS; 587,LLKAAVKGSDGFW" 170:21890473; 868:21890473; 681:21890473; 587:21890473