PTM - CPLM Gene Name UniProt ID Type Position Code USP14; TGT P54578 Acetylation 291 K USP14; TGT P54578 Acetylation 313 K USP14; TGT P54578 Acetylation 449 K USP14; TGT P54578 Ubiquitination 49 K USP14; TGT P54578 Ubiquitination 130 K USP14; TGT P54578 Ubiquitination 214 K USP14; TGT P54578 Ubiquitination 291 K USP14; TGT P54578 Ubiquitination 300 K USP14; TGT P54578 Ubiquitination 380 K USP14; TGT P54578 Ubiquitination 441 K USP14; TGT P54578 Ubiquitination 443 K USP14; TGT P54578 Ubiquitination 449 K USP14; TGT P54578 Ubiquitination 62 K USP14; TGT P54578 Ubiquitination 313 K USP14; TGT P54578 Ubiquitination 336 K USP14; TGT P54578 Ubiquitination 267 K USP14; TGT P54578 Ubiquitination 342 K USP14; TGT P54578 Ubiquitination 400 K USP14; TGT P54578 Ubiquitination 334 K USP14; TGT P54578 Ubiquitination 375 K USP14; TGT P54578 Ubiquitination 54 K USP14; TGT P54578 Ubiquitination 269 K USP14; TGT P54578 Ubiquitination 224 K USP14; TGT P54578 Ubiquitination 238 K USP14; TGT P54578 Ubiquitination 134 K USP14; TGT P54578 Ubiquitination 345 K USP14; TGT P54578 Ubiquitination 348 K USP14; TGT P54578 Ubiquitination 454 K

PTM - dbPAF Gene Name UniProt ID Position Type Species USP14; TGT P54578 136 Y Homo sapiens(Human) USP14; TGT P54578 143 S Homo sapiens(Human) USP14; TGT P54578 148 S Homo sapiens(Human) USP14; TGT P54578 151 Y Homo sapiens(Human) USP14; TGT P54578 222 S Homo sapiens(Human) USP14; TGT P54578 228 S Homo sapiens(Human) USP14; TGT P54578 230 S Homo sapiens(Human) USP14; TGT P54578 232 S Homo sapiens(Human) USP14; TGT P54578 235 T Homo sapiens(Human) USP14; TGT P54578 237 S Homo sapiens(Human) USP14; TGT P54578 276 S Homo sapiens(Human) USP14; TGT P54578 285 Y Homo sapiens(Human) USP14; TGT P54578 299 T Homo sapiens(Human) USP14; TGT P54578 302 S Homo sapiens(Human) USP14; TGT P54578 314 S Homo sapiens(Human) USP14; TGT P54578 315 S Homo sapiens(Human) USP14; TGT P54578 388 T Homo sapiens(Human) USP14; TGT P54578 389 S Homo sapiens(Human) USP14; TGT P54578 393 S Homo sapiens(Human) USP14; TGT P54578 394 S Homo sapiens(Human) USP14; TGT P54578 4 Y Homo sapiens(Human) USP14; TGT P54578 401 Y Homo sapiens(Human) USP14; TGT P54578 405 S Homo sapiens(Human) USP14; TGT P54578 412 S Homo sapiens(Human) USP14; TGT P54578 417 Y Homo sapiens(Human) USP14; TGT P54578 430 S Homo sapiens(Human) USP14; TGT P54578 432 S Homo sapiens(Human) USP14; TGT P54578 436 Y Homo sapiens(Human) USP14; TGT P54578 459 T Homo sapiens(Human) USP14; TGT P54578 492 S Homo sapiens(Human) USP14; TGT P54578 52 T Homo sapiens(Human) USP14; TGT P54578 7 T Homo sapiens(Human) USP14; TGT P54578 74 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P54578 K14-ac Acetylation TVKWGKEkFEGVELN P54578 K49-ac Acetylation ARQKVMVkGGtLkDD P54578 K284-ac Acetylation CFINQEVkyLFTGLk P54578 K291-ac Acetylation kyLFTGLkLrLQEEI P54578 K313-ac Acetylation QRNALYIkssKISRL P54578 K348-ac Acetylation AkVLKDVkFPLMLDM P54578 K391-ac Acetylation QQPNTSDkKSsPQKE P54578 K449-ac Acetylation RkQDEWIkFDDDkVS P54578 K454-ac Acetylation WIkFDDDkVSIVtPE P54578 R293-m1 Methylation LFTGLkLrLQEEItk P54578 Y4-p Phosphorylation ____MPLySVTVKWG P54578 T52-p Phosphorylation KVMVkGGtLkDDDWG P54578 S74-p Phosphorylation MTLLMMGsADALPEE P54578 S83-p Phosphorylation DALPEEPsAKTVFVE P54578 Y136-p Phosphorylation LkDALKRyAGALRAs P54578 S143-p Phosphorylation yAGALRAsGEMAsAQ P54578 S148-p Phosphorylation RAsGEMAsAQyITAA P54578 Y151-p Phosphorylation GEMAsAQyITAALRD P54578 S222-p Phosphorylation LEAIEDDsVkETDss P54578 S228-p Phosphorylation DsVkETDsssAsAAt P54578 S229-p Phosphorylation sVkETDsssAsAAtP P54578 S230-p Phosphorylation VkETDsssAsAAtPs P54578 S232-p Phosphorylation ETDsssAsAAtPskk P54578 T235-p Phosphorylation sssAsAAtPskkKSL P54578 S237-p Phosphorylation sAsAAtPskkKSLID P54578 S276-p Phosphorylation kENQLQLsCFINQEV P54578 Y285-p Phosphorylation FINQEVkyLFTGLkL P54578 T299-p Phosphorylation LrLQEEItkQsPTLQ P54578 S302-p Phosphorylation QEEItkQsPTLQRNA P54578 S314-p Phosphorylation RNALYIkssKISRLP P54578 S315-p Phosphorylation NALYIkssKISRLPA P54578 S394-p Phosphorylation NTSDkKSsPQKEVKY P54578 S412-p Phosphorylation SFADDIGsNNCGyYD P54578 Y417-p Phosphorylation IGsNNCGyYDLQAVL P54578 S432-p Phosphorylation THQGRSSsSGHyVSW P54578 Y436-p Phosphorylation RSSsSGHyVSWVkRk P54578 T459-p Phosphorylation DDkVSIVtPEDILRL P54578 S492-p Phosphorylation VEIMEEEsEQ_____ P54578 K49-ub Ubiquitination ARQKVMVkGGtLkDD P54578 K62-ub Ubiquitination DDDWGNIkIKNGMTL P54578 K130-ub Ubiquitination IRSVPELkDALKRyA P54578 K214-ub Ubiquitination MMRVLQQkLEAIEDD P54578 K224-ub Ubiquitination AIEDDsVkETDsssA P54578 K238-ub Ubiquitination AsAAtPskkKSLIDQ P54578 K239-ub Ubiquitination sAAtPskkKSLIDQF P54578 K269-ub Ubiquitination EEEVTKGkENQLQLs P54578 K284-ub Ubiquitination CFINQEVkyLFTGLk P54578 K291-ub Ubiquitination kyLFTGLkLrLQEEI P54578 K300-ub Ubiquitination rLQEEItkQsPTLQR P54578 K313-ub Ubiquitination QRNALYIkssKISRL P54578 K342-ub Ubiquitination EKESVNAkVLKDVkF P54578 K375-ub Ubiquitination VSFRSKFkDLEDKKV P54578 K441-ub Ubiquitination GHyVSWVkRkQDEWI P54578 K443-ub Ubiquitination yVSWVkRkQDEWIkF P54578 K449-ub Ubiquitination RkQDEWIkFDDDkVS P54578 K454-ub Ubiquitination WIkFDDDkVSIVtPE

PTM - dbPTM UniProt ID Position Code Type Resources References P54578 136 Y Phosphorylation Swiss-Prot 1010711 18669648 P54578 143 S Phosphorylation Swiss-Prot 1010711 19367720;19690332 P54578 291 K Acetylation Swiss-Prot 1010711 19608861 P54578 313 K Acetylation Swiss-Prot 1010711 19608861 P54578 449 K Acetylation Swiss-Prot 1010711 19608861 P54578 130 K Ubiquitylation Phosphositeplus.1010730 21890473 P54578 214 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54578 224 K Ubiquitylation Phosphositeplus.1010730 21906983 P54578 238 K Ubiquitylation Phosphositeplus.1010730 21906983 P54578 291 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54578 300 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54578 313 K Ubiquitylation Phosphositeplus.1010730 21906983 P54578 342 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54578 441 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54578 443 K Ubiquitylation Phosphositeplus.1010730 21890473 P54578 449 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54578 454 K Ubiquitylation Phosphositeplus.1010730 21906983 P54578 49 K Ubiquitylation Phosphositeplus.1010730 21890473 P54578 62 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54578 291 K Acetylation Phosphositeplus.1010730 19608861 P54578 313 K Acetylation Phosphositeplus.1010730 19608861 P54578 449 K Acetylation Phosphositeplus.1010730 19608861 P54578 143 S Phosphorylation Phosphositeplus.1010730 18669648;19362540;19367720;19664995;19690332;19835603;20044836;20068231;20166139;20815410;21406692;21659604;22115753 P54578 148 S Phosphorylation Phosphositeplus.1010730 19835603 P54578 314 S Phosphorylation Phosphositeplus.1010730 20044836 P54578 315 S Phosphorylation Phosphositeplus.1010730 20044836 P54578 235 T Phosphorylation Phosphositeplus.1010730 18234856;20068231 P54578 299 T Phosphorylation Phosphositeplus.1010730 21406692 P54578 136 Y Phosphorylation Phosphositeplus.1010730 18669648 P54578 151 Y Phosphorylation Phosphositeplus.1010730 19835603 P54578 4 Y Phosphorylation Phosphositeplus.1010730 18180459 P54578 436 Y Phosphorylation Phosphositeplus.1010730 20562096 P54578 256 K Acetylation HPRD 9.0 19608861 P54578 291 K Acetylation HPRD 9.0 19608861 P54578 313 K Acetylation HPRD 9.0 19608861 P54578 449 K Acetylation HPRD 9.0 19608861 P54578 143 S Phosphorylation HPRD 9.0 18669648,19664995,19651622,18767875,20166139,20068231 P54578 235 T Phosphorylation HPRD 9.0 18669648,20068231 P54578 136 Y Phosphorylation HPRD 9.0 18669648 P54578 285 Y Phosphorylation HPRD 9.0 18452278

PTM - HPRD Symbol Position Code Type References USP14 101 Y Phosphorylation 18669648 USP14 108 S Phosphorylation 18669648,19664995,19651622,18767875,20166139,20068231 USP14 136 Y Phosphorylation 18669648 USP14 143 S Phosphorylation 18669648,19664995,19651622,18767875,20166139,20068231 USP14 200 T Phosphorylation 18669648,20068231 USP14 235 T Phosphorylation 18669648,20068231 USP14 250 Y Phosphorylation 18452278 USP14 256 K Acetylation 19608861 USP14 278 K Acetylation 19608861 USP14 285 Y Phosphorylation 18452278 USP14 291 K Acetylation 19608861 USP14 313 K Acetylation 19608861 USP14 414 K Acetylation 19608861 USP14 449 K Acetylation 19608861

PTM - UniProt UniProt ID Position Type PMIDs P54578 52 Phosphothreonine 23186163 P54578 143 Phosphoserine 19367720;19690332;20068231;21406692;23186163;24275569 P54578 235 Phosphothreonine 20068231;24275569 P54578 237 Phosphoserine 23186163 P54578 302 Phosphoserine 23186163 P54578 432 Phosphoserine 23186163 P54578 449 N6-acetyllysine 19608861

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type USP14 IPI00219913 K 290 YLFTGLaKLRLQEE acetylation USP14 IPI00219913 K 312 RNALYIaKSSKISR acetylation USP14 IPI00219913 K 448 KQDEWIaKFDDDKV acetylation USP14 IPI00219913 S 142 AGALRApSGEMASA phosphorylation USP14 IPI00219913 S 73 TLLMMGpSADALPE phosphorylation USP14 IPI00640357 T 7 MPLYSVpTVKWGKE phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 9097 114551 - K Ubiquitination 16196087 9097 114551 - K Ubiquitination 22118674 9097 114551 - K Ubiquitination 18781797 9097 114551 - K Ubiquitination 21987572 9097 114551 - K Ubiquitination 21139048 9097 114551 - K Ubiquitination 21890473 9097 114551 - K Ubiquitination 21906983 9097 114551 - K Sumoylation 19471022 9097 114551 - K Ubiquitination 16211010 9097 114551 - K Ubiquitination 22053931 9097 114551 - K Ubiquitination 22505724 9097 114551 - K Ubiquitination 21963094 9097 114551 - K Ubiquitination 23000965 9097 114551 - K Ubiquitination 24654937 9097 114551 - K Ubiquitination 24816145 9097 114551 - K Ubiquitination 25147182

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID USP14 Homo sapiens (Human) 479; 252; 168; 338; 380; 487; 481; 122; 135; 329 "479,HYVSWVKRKQDEW; 252,MRVLQQKLEAIED; 168,RSVPELKDALKRY; 338,LQEEITKQSPTLQ; 380,KESVNAKVLKDVK; 487,KQDEWIKFDDDKV; 481,VSWVKRKQDEWIK; 122,RQKVMVKGGTLKD; 135,DDWGNIKIKNMEL; 329,YLFTGLKLRLQEE" 479:21890473; 252:21890473; 168:21890473; 338:21890473; 380:21890473; 487:21890473; 481:21890473; 122:21890473; 135:21890473; 329:21890473 USP14 Homo sapiens (Human) 49; 256; 307; 408; 414; 179; 95; 265; 406; 62 "49,RQKVMVKGGTLKD; 256,YLFTGLKLRLQEE; 307,KESVNAKVLKDVK; 408,VSWVKRKQDEWIK; 414,KQDEWIKFDDDKV; 179,MRVLQQKLEAIED; 95,RSVPELKDALKRY; 265,LQEEITKQSPTLQ; 406,HYVSWVKRKQDEW; 62,DDWGNIKIKNMEL" 49:21890473; 256:21890473; 307:21890473; 408:21890473; 414:21890473; 179:21890473; 95:21890473; 265:21890473; 406:21890473; 62:21890473 USP14 Homo sapiens (Human) 238; 342; 441; 291; 443; 313; 454; 214; 130; 300; 62; 449; 224 "238,SAATPSKKKSLID; 342,KESVNAKVLKDVK; 441,HYVSWVKRKQDEW; 291,YLFTGLKLRLQEE; 443,VSWVKRKQDEWIK; 313,RNALYIKSSKISR; 454,IKFDDDKVSIVTP; 214,MRVLQQKLEAIED; 130,RSVPELKDALKRY; 300,LQEEITKQSPTLQ; 62,DDWGNIKIKNGMT; 449,KQDEWIKFDDDKV; 224,IEDDSVKETDSSS" "238:21906983; 342:21890473,21906983; 441:21890473,21906983; 291:21890473,21906983; 443:21890473; 313:21906983; 454:21906983; 214:21890473,21906983,22053931; 130:21890473; 300:21890473,21906983; 62:21906983; 449:21890473,21906983; 224:21906983" USP14 Homo sapiens (Human) 342; 441; 49; 291; 443; 214; 130; 300; 449 "342,KESVNAKVLKDVK; 441,HYVSWVKRKQDEW; 49,RQKVMVKGGTLKD; 291,YLFTGLKLRLQEE; 443,VSWVKRKQDEWIK; 214,MRVLQQKLEAIED; 130,RSVPELKDALKRY; 300,LQEEITKQSPTLQ; 449,KQDEWIKFDDDKV" 342:21890473; 441:21890473; 49:21890473; 291:21890473; 443:21890473; 214:21890473; 130:21890473; 300:21890473; 449:21890473 USP14 Homo sapiens (Human) 342; 441; 49; 291; 443; 214; 130; 300; 449 "342,KESVNAKVLKDVK; 441,HYVSWVKRKQDEW; 49,RQKVMVKGGTLKD; 291,YLFTGLKLRLQEE; 443,VSWVKRKQDEWIK; 214,MRVLQQKLEAIED; 130,RSVPELKDALKRY; 300,LQEEITKQSPTLQ; 449,KQDEWIKFDDDKV" 342:21890473; 441:21890473; 49:21890473; 291:21890473; 443:21890473; 214:21890473; 130:21890473; 300:21890473; 449:21890473 USP14 Homo sapiens (Human) 49; 256; 307; 408; 414; 179; 95; 265; 406; 62 "49,RQKVMVKGGTLKD; 256,YLFTGLKLRLQEE; 307,KESVNAKVLKDVK; 408,VSWVKRKQDEWIK; 414,KQDEWIKFDDDKV; 179,MRVLQQKLEAIED; 95,RSVPELKDALKRY; 265,LQEEITKQSPTLQ; 406,HYVSWVKRKQDEW; 62,DDWGNIKIKNMEL" 49:21890473; 256:21890473; 307:21890473; 408:21890473; 414:21890473; 179:21890473; 95:21890473; 265:21890473; 406:21890473; 62:21890473