PTM - CPLM Gene Name UniProt ID Type Position Code USP5; ISOT P45974 Acetylation 184 K USP5; ISOT P45974 Acetylation 357 K USP5; ISOT P45974 Acetylation 353 K USP5; ISOT P45974 Ubiquitination 360 K USP5; ISOT P45974 Ubiquitination 793 K USP5; ISOT P45974 Ubiquitination 423 K USP5; ISOT P45974 Ubiquitination 357 K USP5; ISOT P45974 Ubiquitination 184 K USP5; ISOT P45974 Ubiquitination 318 K USP5; ISOT P45974 Ubiquitination 470 K USP5; ISOT P45974 Ubiquitination 574 K USP5; ISOT P45974 Ubiquitination 575 K USP5; ISOT P45974 Ubiquitination 743 K USP5; ISOT P45974 Ubiquitination 836 K USP5; ISOT P45974 Ubiquitination 845 K USP5; ISOT P45974 Ubiquitination 353 K USP5; ISOT P45974 Ubiquitination 163 K USP5; ISOT P45974 Ubiquitination 558 K USP5; ISOT P45974 Ubiquitination 476 K USP5; ISOT P45974 Ubiquitination 478 K USP5; ISOT P45974 Ubiquitination 516 K USP5; ISOT P45974 Ubiquitination 468 K USP5; ISOT P45974 Ubiquitination 418 K USP5; ISOT P45974 Ubiquitination 178 K USP5; ISOT P45974 Ubiquitination 20 K USP5; ISOT P45974 Ubiquitination 553 K USP5; ISOT P45974 Ubiquitination 586 K USP5; ISOT P45974 Ubiquitination 391 K USP5; ISOT P45974 Ubiquitination 80 K USP5; ISOT P45974 Ubiquitination 291 K USP5; ISOT P45974 Ubiquitination 27 K USP5; ISOT P45974 Ubiquitination 64 K USP5; ISOT P45974 Ubiquitination 406 K USP5; ISOT P45974 Ubiquitination 499 K USP5; ISOT P45974 Ubiquitination 585 K USP5; ISOT P45974 Ubiquitination 247 K USP5; ISOT P45974 Ubiquitination 379 K

PTM - dbPAF Gene Name UniProt ID Position Type Species USP5; ISOT P45974 148 T Homo sapiens(Human) USP5; ISOT P45974 149 S Homo sapiens(Human) USP5; ISOT P45974 156 S Homo sapiens(Human) USP5; ISOT P45974 161 S Homo sapiens(Human) USP5; ISOT P45974 213 T Homo sapiens(Human) USP5; ISOT P45974 216 S Homo sapiens(Human) USP5; ISOT P45974 289 T Homo sapiens(Human) USP5; ISOT P45974 292 T Homo sapiens(Human) USP5; ISOT P45974 294 T Homo sapiens(Human) USP5; ISOT P45974 389 Y Homo sapiens(Human) USP5; ISOT P45974 479 Y Homo sapiens(Human) USP5; ISOT P45974 485 Y Homo sapiens(Human) USP5; ISOT P45974 505 Y Homo sapiens(Human) USP5; ISOT P45974 559 T Homo sapiens(Human) USP5; ISOT P45974 623 T Homo sapiens(Human) USP5; ISOT P45974 652 S Homo sapiens(Human) USP5; ISOT P45974 658 S Homo sapiens(Human) USP5; ISOT P45974 717 S Homo sapiens(Human) USP5; ISOT P45974 779 S Homo sapiens(Human) USP5; ISOT P45974 783 S Homo sapiens(Human) USP5; ISOT P45974 785 S Homo sapiens(Human) USP5; ISOT P45974 787 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P45974 K163-ac Acetylation sADsASRkQEVQAWD P45974 K178-ac Acetylation GEVRQVSkHAFsLkQ P45974 K184-ac Acetylation SkHAFsLkQLDNPAR P45974 K353-ac Acetylation SIPDFQRkYVDkLEk P45974 K357-ac Acetylation FQRkYVDkLEkIFQN P45974 S5-p Phosphorylation ___MAELsEEALLSV P45974 Y57-p Phosphorylation FLGFGKQyVERHFNk P45974 T65-p Phosphorylation VERHFNktGQRVYLH P45974 Y128-p Phosphorylation KIVILPDyLEIARDG P45974 S156-p Phosphorylation SAVEALLsADsASRk P45974 S159-p Phosphorylation EALLsADsASRkQEV P45974 S182-p Phosphorylation QVSkHAFsLkQLDNP P45974 T213-p Phosphorylation ENLWLNLtDGsILCG P45974 S216-p Phosphorylation WLNLtDGsILCGRRY P45974 T289-p Phosphorylation DMLKMQKtDKtMTEL P45974 T292-p Phosphorylation KMQKtDKtMTELEID P45974 Y389-p Phosphorylation HGLLSGEySkPVPES P45974 S453-p Phosphorylation MVERNCRsSENPNEV P45974 Y479-p Phosphorylation LATEkVKyTQRVDyI P45974 Y485-p Phosphorylation KyTQRVDyIMQLPVP P45974 Y505-p Phosphorylation NkEELLEyEEKKRQA P45974 T559-p Phosphorylation AkSVAVktTRFASFP P45974 T623-p Phosphorylation DIAPPLVtPDEPKGS P45974 S658-p Phosphorylation TSPMLDEsVIIQLVE P45974 S779-p Phosphorylation MDISEGRsAAdsIsE P45974 S783-p Phosphorylation EGRsAAdsIsEsVPV P45974 S785-p Phosphorylation RsAAdsIsEsVPVGP P45974 S787-p Phosphorylation AAdsIsEsVPVGPkV P45974 K20-ub Ubiquitination LPTIRVPkAGDRVHk P45974 K27-ub Ubiquitination kAGDRVHkDECAFSF P45974 K64-ub Ubiquitination yVERHFNktGQRVYL P45974 K80-ub Ubiquitination LRRTRRPkEEDPATG P45974 K163-ub Ubiquitination sADsASRkQEVQAWD P45974 K184-ub Ubiquitination SkHAFsLkQLDNPAR P45974 K318-ub Ubiquitination QESGVPLkPLFGPGY P45974 K353-ub Ubiquitination SIPDFQRkYVDkLEk P45974 K357-ub Ubiquitination FQRkYVDkLEkIFQN P45974 K360-ub Ubiquitination kYVDkLEkIFQNAPT P45974 K391-ub Ubiquitination LLSGEySkPVPESGD P45974 K406-ub Ubiquitination GERVPEQkEVQDGIA P45974 K418-ub Ubiquitination GIAPRMFkALIGkGH P45974 K423-ub Ubiquitination MFkALIGkGHPEFST P45974 K468-ub Ubiquitination FRFLVEEkIkCLATE P45974 K470-ub Ubiquitination FLVEEkIkCLATEkV P45974 K476-ub Ubiquitination IkCLATEkVKyTQRV P45974 K499-ub Ubiquitination PMDAALNkEELLEyE P45974 K553-ub Ubiquitination WSTALQAkSVAVktT P45974 K558-ub Ubiquitination QAkSVAVktTRFASF P45974 K574-ub Ubiquitination DYLVIQIkkFTFGLD P45974 K575-ub Ubiquitination YLVIQIkkFTFGLDW P45974 K585-ub Ubiquitination FGLDWVPkkLDVSIE P45974 K586-ub Ubiquitination GLDWVPkkLDVSIEM P45974 K743-ub Ubiquitination FSRDQALkALRATNN P45974 K793-ub Ubiquitination EsVPVGPkVRDGPGK P45974 K836-ub Ubiquitination WVIYNDQkVCASEkP P45974 K842-ub Ubiquitination QkVCASEkPPkDLGY P45974 K845-ub Ubiquitination CASEkPPkDLGYIYF

PTM - dbPTM UniProt ID Position Code Type Resources References P45974 2 A Acetylation Swiss-Prot 1010711 19413330 P45974 184 K Acetylation Swiss-Prot 1010711 19608861 P45974 623 T Phosphorylation Swiss-Prot 1010711 16964243 P45974 783 S Phosphorylation Swiss-Prot 1010711 17924679;19690332 P45974 783 S Phosphorylation PhosphoELM.10011 18669648 P45974 623 T Phosphorylation PhosphoELM.10011 16964243 P45974 767 D Caspase Phosphositeplus.1010730 18722006;18724940 P45974 782 D Caspase Phosphositeplus.1010730 18722006 P45974 163 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 184 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P45974 20 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 27 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 318 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P45974 353 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 357 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P45974 360 K Ubiquitylation Phosphositeplus.1010730 20639865;21890473;21906983 P45974 391 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 406 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 418 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 423 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P45974 468 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 470 K Ubiquitylation Phosphositeplus.1010730 21890473 P45974 476 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 499 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 558 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 574 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 575 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P45974 586 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 64 K Ubiquitylation Phosphositeplus.1010730 21906983 P45974 743 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P45974 793 K Ubiquitylation Phosphositeplus.1010730 20639865;21890473;21906983 P45974 836 K Ubiquitylation Phosphositeplus.1010730 21890473 P45974 184 K Acetylation Phosphositeplus.1010730 19608861 P45974 216 S Phosphorylation Phosphositeplus.1010730 20068231 P45974 783 S Phosphorylation Phosphositeplus.1010730 16964243;18669648;19690332;20058876;20068231;20166139;20562096 P45974 213 T Phosphorylation Phosphositeplus.1010730 20068231 P45974 623 T Phosphorylation Phosphositeplus.1010730 16964243;18510355;20886841 P45974 389 Y Phosphorylation Phosphositeplus.1010730 20562096 P45974 485 Y Phosphorylation Phosphositeplus.1010730 20068231 P45974 505 Y Phosphorylation Phosphositeplus.1010730 20068231 P45974 184 K Acetylation HPRD 9.0 19608861 P45974 161 S Phosphorylation HPRD 9.0 20068231 P45974 216 S Phosphorylation HPRD 9.0 20068231 P45974 779 S Phosphorylation HPRD 9.0 20068231 P45974 783 S Phosphorylation HPRD 9.0 18669648,17924679,18212344,18767875,20166139,20068231,20058876 P45974 213 T Phosphorylation HPRD 9.0 20068231 P45974 623 T Phosphorylation HPRD 9.0 16964243,18510355 P45974 485 Y Phosphorylation HPRD 9.0 20068231 P45974 505 Y Phosphorylation HPRD 9.0 20068231 P45974 783 S Phosphorylation SysPTM 1.1 18212344 P45974 783 S Phosphorylation SysPTM 1.1 16964243 P45974 623 T Phosphorylation SysPTM 1.1 16964243

PTM - HPRD Symbol Position Code Type References USP5 161 S Phosphorylation 20068231 USP5 184 K Acetylation 19608861 USP5 213 T Phosphorylation 20068231 USP5 216 S Phosphorylation 20068231 USP5 485 Y Phosphorylation 20068231 USP5 505 Y Phosphorylation 20068231 USP5 623 T Phosphorylation 16964243,18510355 USP5 635 S Phosphorylation 20068231 USP5 756 S Phosphorylation 20068231 USP5 760 S Phosphorylation 18669648,17924679,18212344,18767875,20166139,20068231,20058876 USP5 779 S Phosphorylation 20068231 USP5 783 S Phosphorylation 18669648,17924679,18212344,18767875,20166139,20068231,20058876

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P45974 623 T 16964243 HTP Phosphorylation P45974 783 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P45974 2 N-acetylalanine 19413330;22223895;22814378 P45974 156 Phosphoserine 24275569 P45974 292 Phosphothreonine 23186163 P45974 779 Phosphoserine 23186163 P45974 783 Phosphoserine 19690332;20068231;23186163 P45974 785 Phosphoserine 23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type USP5 IPI00024664 K 184 KHAFSLaKQLDNPA acetylation USP5 IPI00375145 S 161 LSADSApSRKQEVQ phosphorylation USP5 IPI00375145 S 635 APMLDEpSVIIQLV phosphorylation USP5 IPI00375145 S 756 DISEGRpSAADSIS phosphorylation USP5 IPI00375145 S 760 GRSAADpSISESVP phosphorylation USP5 IPI00375145 Y 485 YTQRVDpYIMQLPV phosphorylation USP5 IPI00375145 Y 505 KEELLEpYEEKKRQ phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 8078 113751 - K Ubiquitination 16196087 8078 113751 - K Ubiquitination 15852347 8078 113751 - K Ubiquitination 20639865 8078 113751 - K Ubiquitination 22118674 8078 113751 - K Ubiquitination 18781797 8078 113751 - K Ubiquitination 21987572 8078 113751 - K Ubiquitination 21963094 8078 113751 - K Ubiquitination 21139048 8078 113751 - K Ubiquitination 21890473 8078 113751 - K Ubiquitination 21906983 8078 113751 - K Sumoylation 19471022 8078 113751 - K Sumoylation 21252943 8078 113751 - K Ubiquitination 22053931 8078 113751 - K Ubiquitination 22505724 8078 113751 - K Ubiquitination 23000965 8078 113751 - K Ubiquitination 24654937 8078 113751 - K Ubiquitination 24816145 8078 113751 - K Sumoylation 25027693 8078 113751 - K Ubiquitination 25015289 8078 113751 - K Ubiquitination 25147182

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID USP5 Homo sapiens (Human) 476; 64; 558; 470; 163; 585; 586; 468; 743; 406; 793; 318; 499; 357; 184; 353; 20; 836; 575; 574; 27; 418; 391; 360; 423; 845 "476,KCLATEKVKYTQR; 64,VERHFNKTGQRVY; 558,AKSVAVKTTRFAS; 470,LVEEKIKCLATEK; 163,ADSASRKQEVQAW; 585,GLDWVPKKLDVSI; 586,LDWVPKKLDVSIE; 468,RFLVEEKIKCLAT; 743,SRDQALKALRATN; 406,ERVPEQKEVQDGI; 793,SVPVGPKVRDGPG; 318,ESGVPLKPLFGPG; 499,MDAALNKEELLEY; 357,QRKYVDKLEKIFQ; 184,KHAFSLKQLDNPA; 353,IPDFQRKYVDKLE; 20,PTIRVPKAGDRVH; 836,VIYNDQKVCASEK; 575,LVIQIKKFTFGLD; 574,YLVIQIKKFTFGL; 27,AGDRVHKDECAFS; 418,IAPRMFKALIGKG; 391,LSGEYSKPVPESG; 360,YVDKLEKIFQNAP; 423,FKALIGKGHPEFS; 845,ASEKPPKDLGYIY" "476:21906983; 64:21906983; 558:21906983; 470:21890473; 163:21906983; 585:21906983; 586:21906983; 468:21906983; 743:21890473,21906983,22053931; 406:21906983; 793:21890473,21906983,22053931; 318:21890473,21906983; 499:21906983; 357:21890473,21906983; 184:21890473,21906983; 353:21906983; 20:21906983; 836:21890473; 575:21890473,21906983; 574:21890473,21906983; 27:21906983; 418:21906983; 391:21906983; 360:21890473,21906983,22053931; 423:21890473,21906983; 845:21890473" USP5 Homo sapiens (Human) 476; 822; 64; 558; 470; 468; 406; 20; 575; 574; 813; 27; 391; 163; 585; 586; 318; 499; 357; 184; 770; 353; 720; 418; 360; 423 "476,KCLATEKVKYTQR; 822,ASEKPPKDLGYIY; 64,VERHFNKTGQRVY; 558,AKSVAVKTTRFAS; 470,LVEEKIKCLATEK; 468,RFLVEEKIKCLAT; 406,ERVPEQKEVQDGI; 20,PTIRVPKAGDRVH; 575,LVIQIKKFTFGLD; 574,YLVIQIKKFTFGL; 813,VIYNDQKVCASEK; 27,AGDRVHKDECAFS; 391,LSGEYSKPVPESG; 163,ADSASRKQEVQAW; 585,GLDWVPKKLDVSI; 586,LDWVPKKLDVSIE; 318,ESGVPLKPLFGPG; 499,MDAALNKEELLEY; 357,QRKYVDKLEKIFQ; 184,KHAFSLKQLDNPA; 770,SVPVGPKVRDGPG; 353,IPDFQRKYVDKLE; 720,SRDQALKALRATN; 418,IAPRMFKALIGKG; 360,YVDKLEKIFQNAP; 423,FKALIGKGHPEFS" "476:21906983; 822:21890473; 64:21906983; 558:21906983; 470:21890473; 468:21906983; 406:21906983; 20:21890473,21906983; 575:21890473,21906983; 574:21890473,21906983; 813:21890473; 27:21906983; 391:21906983; 163:21906983; 585:21906983; 586:21906983; 318:21890473,21906983; 499:21906983; 357:21890473,21906983; 184:21890473,21906983; 770:21890473,21906983; 353:21906983; 720:21890473,21906983; 418:21906983; 360:21890473,21906983; 423:21890473,21906983" USP5 Homo sapiens (Human) 357; 743; 360; 423; 793 "357,QRKYVDKLEKIFQ; 743,SRDQALKALRATN; 360,YVDKLEKIFQNAP; 423,FKALIGKGHPEFS; 793,SVPVGPKVRDGPG" "357:21139048; 743:22053931; 360:21139048,22053931; 423:21139048; 793:21139048,22053931"