PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus GALNT15 SPSB2 21988832 biogrid,intact exp GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G SPSB2 DDX4 20561531 biogrid exp GP MET SPSB2 15713673 biogrid exp GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G TTC1 SPSB2 26186194 biogrid exp GP GP G G G GP G G P G GP GP GP G GP GP G G G GP GP G GP G GP GP G NOS2 SPSB2 23438482,21549100,20603330,21199876 biogrid exp P P G G P G P P P P GP GP G GP G P P GP P P G SPSB2 PRMT1 23455924 biogrid,intact exp GP GP G G G GP G G P G GP G GP G GP GP G G G GP GP G GP G GP GP G EPHB2 SPSB2 26186194 biogrid exp P GP G G G GP G G P G GP P GP GP GP G GP G P GP G GP P P GP G ARHGEF10 SPSB2 26186194 biogrid exp GP GP G GP G G G GP G G G GP G P G GP G G G GP G GP G PAWR SPSB2 20561531 biogrid,intact exp GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G APP SPSB2 21832049 biogrid exp G GP G G G GP G G P G G G G G GP GP G GP G GP G G GP G G GP G PIK3R3 SPSB2 25814554 biogrid,intact exp GP GP G G G GP G G P G GP GP GP G GP GP G GP G G GP G GP GP GP GP G SPSB2 RNF7 15601820, 25402006 innatedb,biogrid,intact exp, pred GP GP G G G GP G G P G GP GP G G GP GP G GP G GP G G GP GP GP GP G CUL5 SPSB2 26186194,15601820, 25402006 innatedb,biogrid,intact exp, pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G TCEB2 SPSB2 15601820, 25402006 innatedb,biogrid,intact exp, pred G G G G G G G G G G G G G G G G G G G G G G G G G G TCEB1 SPSB2 19159283,15601820,21199876, 25402006 innatedb,biogrid,intact exp, pred G G G G G G G G G G G G G G G G G G G G G G G G G G COPS6 SPSB2 25402006 pred GP GP G G G GP G G P G GP G GP G GP GP G GP G GP GP G GP GP GP GP G RGL4 SPSB2 16082366 pred P G G P P P G P P P P G P SOCS4 SPSB2 25402006 pred GP GP G G G GP G G P G GP G GP G GP GP G G G GP GP G GP GP GP GP G SPSB4 SPSB2 25402006, 16082366 pred G TRIM38 SPSB2 16082366 pred GP GP G G P G G P GP GP GP G GP GP G GP GP GP G GP GP GP P G RGL2 SPSB2 16082366 pred GP GP G G G GP G G P G GP GP G G GP GP G GP G GP G G GP G GP GP G SPSB2 FEM1B 25402006 pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP G GP GP G ASB2 SPSB2 25402006 pred P P P G P P P P G P P GP P G G P G P G SOS1 SPSB2 16082366 pred GP G G G G G G G P G GP G GP G GP G G GP G GP GP G GP GP GP G G GPS2 SPSB2 16082366 pred G G G G G G G G G G G G G G G G G G G G G G G G G G CUL2 SPSB2 25402006 pred GP GP G G G GP G G P G GP GP GP G G GP G GP G GP GP G G G GP GP G RBX1 SPSB2 25402006 pred G G G G G GP G G G G G GP G G GP G GP G GP G G G G G GP G SOCS3 SPSB2 25402006 pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G G GP G GP G GP GP G BCAR3 SPSB2 16082366 pred GP GP G GP G G P G GP GP GP G GP GP G P G GP GP G GP G GP GP G SOS2 SPSB2 16082366 pred GP GP G G G GP G G P G GP G GP G GP GP G GP G GP GP G GP GP GP GP G RALGDS SPSB2 16082366 pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G RASGEF1B SPSB2 16082366 pred P P P P G P P P P P P P P RASGEF1C SPSB2 16082366 pred P P G GP P G P P P P GP GP P P P P GP RALGPS2 SPSB2 16082366 pred GP GP G G P G P GP GP P G GP P G P GP P GP GP GP GP SH2D3C SPSB2 16082366 pred GP G G G G G G G G GP G G G G G G G G G G G GP GP GP G LRRC41 SPSB2 25402006 pred G G G G G G G G P G GP G GP G GP G G GP G G G G GP G GP GP G RAPGEF3 SPSB2 16082366 pred GP P G GP G G P G GP P GP G P GP G GP GP GP G P GP GP P G SOCS5 SPSB2 25402006 pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP G GP GP G SPSB2 G3BP2 16082366 pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G RAPGEF1 SPSB2 16082366 pred G GP G G G GP G G P G GP G GP G GP GP G GP G GP G G GP GP GP GP G SPSB2 TRIM17 16082366 pred P P P P P P P P P P P P P GP SPSB2 RAPGEFL1 16082366 pred G G GP P G P P GP GP P P P P G3BP1 SPSB2 16082366 pred GP GP G G G GP G G P G GP G GP G GP GP G GP G GP GP G GP GP GP GP G MED8 SPSB2 25402006 pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G RASGRF2 SPSB2 16082366 pred GP GP G G G GP G G P G GP P GP G GP GP G GP G GP P G GP GP GP GP G LRR1 SPSB2 25402006 pred G G G G G G G G G G G G G G G G G G G G G G G G G NEDD8 SPSB2 25402006 pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G SPSB1 SPSB2 25402006, 16082366 pred GP GP G G G GP G G G GP GP GP G G GP G GP G GP G G G GP GP G RAB40C SPSB2 25402006 pred G G G G G GP G G P G GP G GP G GP GP G GP G G G G GP G GP GP G VHL SPSB2 25402006 pred G G G G G G G G G GP GP GP G G G G P G GP GP G G G G GP G SPSB2 TRIM39 16082366 pred P P P P P P P P P P SPSB2 ULBP2 16082366 pred P G GP G P G G GP G SPSB2 RGL1 16082366 pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G RASGRP1 SPSB2 16082366 pred G G G G G G G G G G G G G G G G RASGRP2 SPSB2 16082366 pred G GP G G G GP G P G GP GP GP G GP GP P G P G GP GP GP P G SOCS2 SPSB2 25402006 pred GP GP G G G GP G G P G GP GP GP G GP GP G GP G GP GP G GP GP GP GP G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q96IZ0 Q99619 unspecified method 20561531 direct interaction X 2 Q61496 Q99619 x-ray crystallography 20561531 association X 2 Q99619 Q9NQI0-4 unspecified method 20561531 direct interaction X 2 Q99619 Q15369-2 two hybrid 19159283 direct interaction X 2 Q99619 Q15369-2 affinity chromatography technology 19159283 physical association X 2 Q99619 Q9UBF6-2 affinity chromatography technology 15601820 physical association X 2 Q99619 Q15369-2 affinity chromatography technology 15601820 physical association X 2 Q99619 Q15369-2 affinity chromatography technology 21199876 physical association X 2 Q99619 B4DLF5 affinity chromatography technology 15713673 physical association X 2 Q99619 P05067-7 pull down 21832049 direct interaction X 2 Q99619 Q93034 affinity chromatography technology 15601820 physical association X 2 Q99619 Q15370-2 affinity chromatography technology 15601820 physical association X 2 Q99619 A0A087X1W2 affinity chromatography technology 23455924 physical association X 2 Q99619 A0A087X1W2 two hybrid 23455924 direct interaction X 2 Q8N3T1 Q99619 two hybrid 21988832 direct interaction X 2 P35228 Q99619 affinity chromatography technology 23438482 physical association X 2 P35228 Q99619 enzymatic study 20603330 direct interaction X 2 P35228 Q99619 affinity chromatography technology 21199876 physical association X 2 P35228 Q99619 affinity chromatography technology 21199876 physical association X 2 P35228 Q99619 affinity chromatography technology 21549100 physical association X 2 P35228 Q99619 pull down 21549100 direct interaction X 2 P35228 Q99619 affinity chromatography technology 21549100 physical association X 2 P35228 Q99619 pull down 20603330 direct interaction X 2 P35228 Q99619 affinity chromatography technology 20603330 physical association X 2 Q99619 anti tag coimmunoprecipitation 15601820 association C 5 Q99619 anti tag coimmunoprecipitation 15601820 association C 5 Q96IZ0 Q99619 isothermal titration calorimetry 20561531 direct interaction X 2 A0A024R7Y5 Q99619 anti tag coimmunoprecipitation 19159283 physical association X 2 P09052 Q99619 isothermal titration calorimetry 20561531 direct interaction X 2 P09052 Q99619 x-ray crystallography 20561531 direct interaction X 2 Q99873 Q99619 anti tag coimmunoprecipitation 23455924 physical association X 2 Q99873 Q99619 two hybrid 23455924 physical association X 2 Q8N3T1 Q99619 two hybrid array 21988832 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B PAWR SPSB2 MI:0686(unspecified method)|MI:0114(x-ray crystallography)|MI:0114(x-ray crystallography)|MI:0114(x-ray crystallography) 20561531 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction) (biogrid)|(intact)|(intact)|(intact) BIOGRID_196988|UniProt_43878|UniProt_106964|UniProt_170273 prey|neutral component|neutral component|neutral component bait|neutral component|neutral component|neutral component APP SPSB2 MI:0096(pull down) 21832049 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_228605 prey bait SPSB2 DDX4 MI:0686(unspecified method) 20561531 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_196987 bait prey NOS2 SPSB2 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0096(pull down)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0096(pull down) 20603330,21199876,21549100,23438482 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0407(direct interaction) (biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid) BIOGRID_258343|BIOGRID_146926|BIOGRID_147098|BIOGRID_146937|BIOGRID_163762|BIOGRID_147097|BIOGRID_147096|BIOGRID_163761 bait|bait|bait|prey|prey|prey|prey|prey prey|prey|prey|bait|bait|bait|bait|bait GALNTL2 SPSB2 MI:0397(two hybrid array)|MI:0004(affinity chromatography technology) 21988832 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction) (intact)|(biogrid) UniProt_118459|BIOGRID_285637 unspecified role|bait unspecified role|prey SPSB2 RNF7 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation) 15601820 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0914(association)|MI:0914(association)|MI:0914(association)|MI:0914(association) (biogrid)|(intact)|(intact)|(intact)|(intact) BIOGRID_250331|UniProt_164456|UniProt_80339|UniProt_80328|UniProt_164445 bait|bait|bait|bait|bait prey|prey|prey|prey|prey TCEB1 SPSB2 MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0004(affinity chromatography technology)|MI:0231(mammalian protein protein interaction trap)|MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation) 15601820,19159283,21199876 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0914(association)|MI:0914(association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0914(association)|MI:0914(association) (intact)|(intact)|(biogrid)|(biogrid)|(biogrid)|(intact)|(biogrid)|(intact)|(intact) UniProt_164456|UniProt_80339|BIOGRID_197020|BIOGRID_197009|BIOGRID_146930|UniProt_98782|BIOGRID_250333|UniProt_80328|UniProt_164445 prey|prey|prey|bait|bait|prey|prey|prey|prey bait|bait|bait|prey|prey|bait|bait|bait|bait TCEB2 SPSB2 MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation) 15601820 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0914(association)|MI:0914(association)|MI:0915(physical association)|MI:0914(association)|MI:0914(association) (intact)|(intact)|(biogrid)|(intact)|(intact) UniProt_164456|UniProt_80339|BIOGRID_250332|UniProt_80328|UniProt_164445 prey|prey|prey|prey|prey bait|bait|bait|bait|bait SPSB2 PRMT1 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 23455924 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction) (intact)|(intact)|(biogrid)|(biogrid) UniProt_129694|UniProt_129837|BIOGRID_274701|BIOGRID_297717 prey|bait|bait|bait bait|prey|prey|prey CUL5 SPSB2 MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 15601820 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0914(association)|MI:0914(association)|MI:0914(association)|MI:0914(association)|MI:0915(physical association) (intact)|(intact)|(intact)|(intact)|(biogrid) UniProt_164456|UniProt_80339|UniProt_80328|UniProt_164445|BIOGRID_250330 prey|prey|prey|prey|prey bait|bait|bait|bait|bait MET SPSB2 MI:0004(affinity chromatography technology) 15713673 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_200409 bait prey

PPI - HINT Gene A Gene B PMID Method Quality ARHGEF10 SPSB2 26186194 0004 HT EPHB2 SPSB2 26186194 0004 HT NOS2 SPSB2 20603330,21549100,21199876,21549100,23438482,20603330,20603330 0096,0004,0004,0096,0004,0004,0415 LC TCEB1 SPSB2 21199876,15601820,19159283,19159283,19159283,15601820 0004,0007,0004,0007,0018,0004 LC CUL5 SPSB2 15601820,15601820,26186194 0004,0007,0004 LC,HT TTC1 SPSB2 26186194 0004 HT ARHGEF10 SPSB2 26186194 0004 HT EPHB2 SPSB2 26186194 0004 HT CUL5 SPSB2 26186194 0004 HT TTC1 SPSB2 26186194 0004 HT NOS2 SPSB2 20603330,21549100,21199876,21549100,23438482,20603330,20603330 0096,0004,0004,0096,0004,0004,0415 LC TCEB1 SPSB2 21199876,15601820,19159283,19159283,19159283,15601820 0004,0007,0004,0007,0018,0004 LC CUL5 SPSB2 15601820,15601820 0004,0007 LC NOS2 SPSB2 20603330,20603330,20603330 0096,0004,0415 LC TCEB1 SPSB2 19159283,19159283,19159283 0004,0007,0018 LC GALNT15 SPSB2 21988832,21988832 0397,0018 HT PIK3R3 SPSB2 25814554,25814554,25814554,25814554,25814554 0729,1356,0397,0018,0004 HT PAWR SPSB2 20561531 0065 LC SPSB2 PRMT1 23455924,23455924,23455924 0018,0007,0004 HT

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q99619 P08581 SPSB2 MET 0.21 15713673 Q99619 Q92569 SPSB2 PIK3R3 0.523 25814554 Q99619 P09052 SPSB2 VAS 0.523 20561531 Q99619 Q15370 SPSB2 TCEB2 0.236 15601820 Q9UBF6 Q99619 RNF7 SPSB2 0.236 15601820 Q99619 P35228 SPSB2 NOS2 0.911 23438482,20603330,21199876,21549100 Q99619 Q15369 SPSB2 TCEB1 0.74 19159283,15601820,21199876 Q99619 Q93034 SPSB2 CUL5 0.332 15601820,26186194 Q99619 Q96IZ0 SPSB2 PAWR 0.523 20561531 Q99873 Q99619 PRMT1 SPSB2 0.49 23455924 Q99619 Q99614 SPSB2 TTC1 0.126 26186194 Q99619 P29323 SPSB2 EPHB2 0.126 26186194 Q99619 P05067 SPSB2 APP 0.183 21832049 Q99619 O15013 SPSB2 ARHGEF10 0.126 26186194 Q99619 Q61496 SPSB2 DDX4 0.309 20561531 Q99619 Q8N3T1 SPSB2 GALNT15 0.286 21988832 Q9NQI0 Q99619 DDX4 SPSB2 0.309 20561531

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 ARHGEF10 SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database APP SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database MET SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database EPHB2 SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database NOS2 SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database TCEB1 SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database TCEB2 SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database GALNT15 SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database PIK3R3 SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database CUL5 SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database PAWR SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database TTC1 SPSB2 MI:0045 experimental interaction detection MI:0461 interaction database SPSB2 PRMT1 MI:0045 experimental interaction detection MI:0461 interaction database SPSB2 DDX4 MI:0362 inference MI:0461 interaction database SPSB2 RNF7 MI:0045 experimental interaction detection MI:0461 interaction database