PTM - CPLM Gene Name UniProt ID Type Position Code APAF1; KIAA0413 O14727 Ubiquitination 820 K APAF1; KIAA0413 O14727 Ubiquitination 756 K APAF1; KIAA0413 O14727 Ubiquitination 198 K APAF1; KIAA0413 O14727 Ubiquitination 267 K APAF1; KIAA0413 O14727 Ubiquitination 277 K APAF1; KIAA0413 O14727 Ubiquitination 380 K APAF1; KIAA0413 O14727 Ubiquitination 768 K APAF1; KIAA0413 O14727 Ubiquitination 224 K APAF1; KIAA0413 O14727 Ubiquitination 733 K APAF1; KIAA0413 O14727 Ubiquitination 643 K APAF1; KIAA0413 O14727 Ubiquitination 916 K APAF1; KIAA0413 O14727 Ubiquitination 290 K APAF1; KIAA0413 O14727 Ubiquitination 288 K

PTM - dbPAF Gene Name UniProt ID Position Type Species APAF1; KIAA0413 O14727 1103 T Homo sapiens(Human) APAF1; KIAA0413 O14727 1108 T Homo sapiens(Human) APAF1; KIAA0413 O14727 204 T Homo sapiens(Human) APAF1; KIAA0413 O14727 238 S Homo sapiens(Human) APAF1; KIAA0413 O14727 24 Y Homo sapiens(Human) APAF1; KIAA0413 O14727 248 S Homo sapiens(Human) APAF1; KIAA0413 O14727 268 S Homo sapiens(Human) APAF1; KIAA0413 O14727 272 S Homo sapiens(Human) APAF1; KIAA0413 O14727 278 Y Homo sapiens(Human) APAF1; KIAA0413 O14727 31 S Homo sapiens(Human) APAF1; KIAA0413 O14727 320 S Homo sapiens(Human) APAF1; KIAA0413 O14727 357 S Homo sapiens(Human) APAF1; KIAA0413 O14727 369 S Homo sapiens(Human) APAF1; KIAA0413 O14727 371 S Homo sapiens(Human) APAF1; KIAA0413 O14727 382 Y Homo sapiens(Human) APAF1; KIAA0413 O14727 383 Y Homo sapiens(Human) APAF1; KIAA0413 O14727 48 T Homo sapiens(Human) APAF1; KIAA0413 O14727 901 S Homo sapiens(Human) APAF1; KIAA0413 O14727 905 T Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA O14727 K643-ac Acetylation DKTLQVFkAETGEKL O14727 K654-ac Acetylation GEKLLEIkAHEDEVL O14727 Y24-p Phosphorylation EKDIKTSyIMDHMIs O14727 S31-p Phosphorylation yIMDHMIsDGFLTIS O14727 T48-p Phosphorylation EKVRNEPtQQQRAAM O14727 S161-p Phosphorylation GMAGCGKsVLAAEAV O14727 S238-p Phosphorylation MLRKHPRsLLILDDV O14727 S248-p Phosphorylation ILDDVWDsWVLKAFD O14727 S268-p Phosphorylation LLTTRDKsVTDsVMG O14727 S272-p Phosphorylation RDKsVTDsVMGPKyV O14727 Y278-p Phosphorylation DsVMGPKyVVPVESS O14727 S320-p Phosphorylation IIKECKGsPLVVSLI O14727 S357-p Phosphorylation FKRIRKSsSYDYEAL O14727 S369-p Phosphorylation EALDEAMsIsVEMLR O14727 S371-p Phosphorylation LDEAMsIsVEMLRED O14727 Y382-p Phosphorylation LREDIKDyyTDLSIL O14727 Y383-p Phosphorylation REDIKDyyTDLSILQ O14727 S506-p Phosphorylation ELCALMFsLDWIKAK O14727 S901-p Phosphorylation GSSFLTSsDDQtIRL O14727 T905-p Phosphorylation LTSsDDQtIRLWETK O14727 T1103-p Phosphorylation DATKFSStSADKtAK O14727 T1108-p Phosphorylation SStSADKtAKIWSFD O14727 K224-ub Ubiquitination PLNIEEAkDRLRILM O14727 K288-ub Ubiquitination PVESSLGkEKGLEIL O14727 K733-ub Ubiquitination KLWDLNQkECRNTMF

PTM - dbPTM UniProt ID Position Code Type Resources References O14727 204 T Phosphorylation Swiss-Prot 1010711 17081983 O14727 238 S Phosphorylation Swiss-Prot 1010711 17081983 O14727 248 S Phosphorylation Swiss-Prot 1010711 17081983 O14727 238 S Phosphorylation PhosphoELM.10011 17081983 O14727 248 S Phosphorylation PhosphoELM.10011 17081983 O14727 204 T Phosphorylation PhosphoELM.10011 17081983 O14727 224 K Ubiquitylation Phosphositeplus.1010730 21906983 O14727 288 K Ubiquitylation Phosphositeplus.1010730 21906983 O14727 238 S Phosphorylation Phosphositeplus.1010730 17081983;20068231;21406692 O14727 248 S Phosphorylation Phosphositeplus.1010730 17081983;20068231;21406692 O14727 268 S Phosphorylation Phosphositeplus.1010730 22246185 O14727 357 S Phosphorylation Phosphositeplus.1010730 22246185 O14727 238 S Phosphorylation HPRD 9.0 17081983,20068231 O14727 248 S Phosphorylation HPRD 9.0 17081983,20068231 O14727 204 T Phosphorylation HPRD 9.0 17081983 O14727 238 S Phosphorylation SysPTM 1.1 17081983 O14727 248 S Phosphorylation SysPTM 1.1 17081983 O14727 204 T Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References APAF1 193 T Phosphorylation 17081983 APAF1 204 T Phosphorylation 17081983 APAF1 227 S Phosphorylation 17081983,20068231 APAF1 237 S Phosphorylation 17081983,20068231 APAF1 238 S Phosphorylation 17081983,20068231 APAF1 248 S Phosphorylation 17081983,20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type O14727 238 S 17081983 HTP Phosphorylation O14727 248 S 17081983 HTP Phosphorylation O14727 204 T 17081983 HTP Phosphorylation

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type APAF1 IPI00023630 S 238 LRKHPRpSLLILDD phosphorylation APAF1 IPI00023630 S 248 LDDVWDpSWVLKAF phosphorylation APAF1 IPI00023630 T 204 KLQNLCpTRLDQDE phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 317 106814 - K Ubiquitination 21139048 317 106814 - K Ubiquitination 21890473 317 106814 - K Ubiquitination 21906983 317 106814 - K Ubiquitination 24816145

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID APAF1 Homo sapiens (Human) 820 "820,MVAAKNKIFLWNT" 820:21890473 APAF1 Homo sapiens (Human) 820 "820,MVAAKNKIFLWNT" 820:21890473 APAF1 Homo sapiens (Human) 277; 213; 809 "277,VESSLGKEKGLEI; 213,LNIEEAKDRLRIL; 809,MVAAKNKIFLWNT" 277:21906983; 213:21906983; 809:21890473 APAF1 Homo sapiens (Human) 288; 820; 224 "288,VESSLGKEKGLEI; 820,MVAAKNKIFLWNT; 224,LNIEEAKDRLRIL" 288:21906983; 820:21890473; 224:21906983 APAF1 Homo sapiens (Human) 288; 828; 224 "288,VESSLGKEKGLEI; 828,MVAAKNKIFLWNT; 224,LNIEEAKDRLRIL" 288:21906983; 828:21890473; 224:21906983 APAF1 Homo sapiens (Human) 288; 224 "288,VESSLGKEKGLEI; 224,LNIEEAKDRLRIL" 288:21906983; 224:21906983 APAF1 Homo sapiens (Human) 277; 213 "277,VESSLGKEKGLEI; 213,LNIEEAKDRLRIL" 277:21906983; 213:21906983 APAF1 Homo sapiens (Human) 288; 224 "288,VESSLGKEKGLEI; 224,LNIEEAKDRLRIL" 288:21906983; 224:21906983