PTM - CPLM Gene Name UniProt ID Type Position Code WDR74; NSA1 Q6RFH5 Ubiquitination 68 K WDR74; NSA1 Q6RFH5 Ubiquitination 156 K WDR74; NSA1 Q6RFH5 Ubiquitination 197 K WDR74; NSA1 Q6RFH5 Ubiquitination 266 K WDR74; NSA1 Q6RFH5 Ubiquitination 359 K WDR74; NSA1 Q6RFH5 Ubiquitination 281 K WDR74; NSA1 Q6RFH5 Ubiquitination 28 K WDR74; NSA1 Q6RFH5 Ubiquitination 115 K WDR74; NSA1 Q6RFH5 Ubiquitination 117 K WDR74; NSA1 Q6RFH5 Ubiquitination 311 K WDR74; NSA1 Q6RFH5 Ubiquitination 357 K WDR74; NSA1 Q6RFH5 Ubiquitination 307 K WDR74; NSA1 Q6RFH5 Ubiquitination 151 K WDR74; NSA1 Q6RFH5 Ubiquitination 21 K WDR74; NSA1 Q6RFH5 Ubiquitination 337 K

PTM - dbPAF Gene Name UniProt ID Position Type Species WDR74; NSA1 Q6RFH5 148 T Homo sapiens(Human) WDR74; NSA1 Q6RFH5 204 Y Homo sapiens(Human) WDR74; NSA1 Q6RFH5 214 S Homo sapiens(Human) WDR74; NSA1 Q6RFH5 312 S Homo sapiens(Human) WDR74; NSA1 Q6RFH5 320 S Homo sapiens(Human) WDR74; NSA1 Q6RFH5 361 S Homo sapiens(Human) WDR74; NSA1 Q6RFH5 382 S Homo sapiens(Human) WDR74; NSA1 Q6RFH5 383 T Homo sapiens(Human) WDR74; NSA1 Q6RFH5 384 S Homo sapiens(Human) WDR74; NSA1 Q6RFH5 52 T Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q6RFH5 K311-m1 Methylation LEHKVYLksQLNCLL Q6RFH5 T345-gl O-GlcNAc VPLEDTEtDELWAsL Q6RFH5 S351-gl O-GlcNAc EtDELWAsLEAAAkR Q6RFH5 T52-p Phosphorylation VSALCWGtGGETQML Q6RFH5 S71-p Phosphorylation DRTVkHFsTEDGIFQ Q6RFH5 Y204-p Phosphorylation kLVTCTGyHQVRVYD Q6RFH5 S214-p Phosphorylation VRVYDPAsPQRRPVL Q6RFH5 S312-p Phosphorylation EHKVYLksQLNCLLL Q6RFH5 S320-p Phosphorylation QLNCLLLsGRDNWED Q6RFH5 S361-p Phosphorylation AAAkRkLsGLEQPQG Q6RFH5 S382-p Phosphorylation RKKKRPGstsP____ Q6RFH5 T383-p Phosphorylation KKKRPGstsP_____ Q6RFH5 S384-p Phosphorylation KKRPGstsP______ Q6RFH5 K21-sm Sumoylation GTETGILkGVNLQRK Q6RFH5 K156-sm Sumoylation GGkENALkIWDLQGS Q6RFH5 K359-sm Sumoylation LEAAAkRkLsGLEQP Q6RFH5 K68-ub Ubiquitination GCADRTVkHFsTEDG Q6RFH5 K151-ub Ubiquitination HVVATGGkENALkIW Q6RFH5 K156-ub Ubiquitination GGkENALkIWDLQGS Q6RFH5 K197-ub Ubiquitination QFLPGSQkLVTCTGy Q6RFH5 K266-ub Ubiquitination GRLLGCLkGLAGSVR Q6RFH5 K281-ub Ubiquitination GLQCHPSkPLLASCG Q6RFH5 K337-ub Ubiquitination QEPQEPNkVPLEDTE Q6RFH5 K357-ub Ubiquitination AsLEAAAkRkLsGLE Q6RFH5 K359-ub Ubiquitination LEAAAkRkLsGLEQP

PTM - dbPTM UniProt ID Position Code Type Resources References Q6RFH5 361 S Phosphorylation Swiss-Prot 1010711 18669648 Q6RFH5 361 S Phosphorylation PhosphoELM.10011 18669648 Q6RFH5 156 K Ubiquitylation Phosphositeplus.1010730 21906983 Q6RFH5 266 K Ubiquitylation Phosphositeplus.1010730 21906983 Q6RFH5 337 K Ubiquitylation Phosphositeplus.1010730 21906983 Q6RFH5 357 K Ubiquitylation Phosphositeplus.1010730 21906983 Q6RFH5 359 K Ubiquitylation Phosphositeplus.1010730 20972266;21906983 Q6RFH5 68 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q6RFH5 214 S Phosphorylation Phosphositeplus.1010730 20068231 Q6RFH5 312 S Phosphorylation Phosphositeplus.1010730 21406692 Q6RFH5 320 S Phosphorylation Phosphositeplus.1010730 21406692 Q6RFH5 361 S Phosphorylation Phosphositeplus.1010730 18669648;19276368;19691289;20068231;20815410 Q6RFH5 384 S Phosphorylation Phosphositeplus.1010730 20068231;21406692 Q6RFH5 383 T Phosphorylation Phosphositeplus.1010730 21406692 Q6RFH5 214 S Phosphorylation HPRD 9.0 20068231 Q6RFH5 361 S Phosphorylation HPRD 9.0 18669648,19691289,20068231 Q6RFH5 382 S Phosphorylation HPRD 9.0 17081983,19651622 Q6RFH5 384 S Phosphorylation HPRD 9.0 17081983,19651622,20068231 Q6RFH5 361 S Phosphorylation SysPTM 1.1 18669648 Q6RFH5 382 S Phosphorylation SysPTM 1.1 17081983 Q6RFH5 384 S Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References WDR74 214 S Phosphorylation 20068231 WDR74 361 S Phosphorylation 18669648,19691289,20068231 WDR74 382 S Phosphorylation 17081983,19651622 WDR74 384 S Phosphorylation 17081983,19651622,20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q6RFH5 361 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q6RFH5 214 Phosphoserine 20068231;23186163 Q6RFH5 311 N6-methyllysine 24129315 Q6RFH5 361 Phosphoserine 18669648;20068231;23186163;24275569

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type WDR74 IPI00018192 S 361 AAKRKLpSGLEQPQ phosphorylation WDR74 IPI00018192 S 382 KKKRPGpSTSP___ phosphorylation WDR74 IPI00018192 S 384 KRPGSTpSP_____ phosphorylation WDR74 IPI00018192 T 148 HPHVVApTGGKENA phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 54663 120092 - K Ubiquitination 21987572 54663 120092 - K Ubiquitination 20972266 54663 120092 - K Ubiquitination 21890473 54663 120092 - K Ubiquitination 21906983 54663 120092 - K Ubiquitination 22505724 54663 120092 - K FAT10ylation 22797925 54663 120092 - K Ubiquitination 21963094 54663 120092 - K Ubiquitination 24816145

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID WDR74 Homo sapiens (Human) 68 "68,CADRTVKHFSTED" 68:21890473 WDR74 Homo sapiens (Human) 68; 357; 359; 156; 337; 266 "68,CADRTVKHFSTED; 357,SLEAAAKRKLSGL; 359,EAAAKRKLSGLEQ; 156,GKENALKIWDLQG; 337,EPQEPNKVPLEDT; 266,RLLGCLKGLAGSV" "68:21890473,21906983; 357:21906983; 359:21906983; 156:21906983; 337:21906983; 266:21906983" WDR74 Homo sapiens (Human) 68; 340; 156; 266 "68,CADRTVKHFSTED; 340,EAAAKRKLSGLEQ; 156,GKENALKIWDLQG; 266,RLLGCLKGLAGSV" "68:21890473,21906983; 340:21906983; 156:21906983; 266:21906983" WDR74 Homo sapiens (Human) 359 "359,EAAAKRKLSGLEQ" 359:20972266