PTM - CPLM Gene Name UniProt ID Type Position Code RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 335 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 1097 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 43 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 108 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 1221 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 297 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 603 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 1157 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 932 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 97 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 1008 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 840 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 120 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 131 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 973 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 1056 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 1104 K RPTOR; KIAA1303; RAPTOR Q8N122 Ubiquitination 511 K

PTM - dbPAF Gene Name UniProt ID Position Type Species RPTOR; KIAA1303; RAPTOR Q8N122 1189 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 1193 Y Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 122 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 159 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 692 Y Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 696 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 699 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 700 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 704 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 706 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 711 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 714 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 719 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 721 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 722 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 723 Y Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 738 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 771 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 792 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 793 Y Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 795 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 8 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 853 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 854 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 855 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 857 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 859 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 863 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 865 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 877 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 881 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 882 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 883 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 884 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 885 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 886 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 887 S Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 889 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 908 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 916 Y Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 917 T Homo sapiens(Human) RPTOR; KIAA1303; RAPTOR Q8N122 982 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q8N122 R718-m1 Methylation sPCtPRLrsVssyGN Q8N122 S8-p Phosphorylation MESEMLQsPLLGLGE Q8N122 S48-p Phosphorylation GSKSLAQsWRMKDRM Q8N122 T159-p Phosphorylation GHGVPRPtVNGEVWV Q8N122 Y692-p Phosphorylation FIEEEKNyALPsPAt Q8N122 S696-p Phosphorylation EKNyALPsPAttEGG Q8N122 T699-p Phosphorylation yALPsPAttEGGsLt Q8N122 T700-p Phosphorylation ALPsPAttEGGsLtP Q8N122 S704-p Phosphorylation PAttEGGsLtPVRDs Q8N122 T706-p Phosphorylation ttEGGsLtPVRDsPC Q8N122 S711-p Phosphorylation sLtPVRDsPCtPRLr Q8N122 T714-p Phosphorylation PVRDsPCtPRLrsVs Q8N122 S719-p Phosphorylation PCtPRLrsVssyGNI Q8N122 S721-p Phosphorylation tPRLrsVssyGNIRA Q8N122 S722-p Phosphorylation PRLrsVssyGNIRAV Q8N122 Y723-p Phosphorylation RLrsVssyGNIRAVA Q8N122 S738-p Phosphorylation TARSLNKsLQNLSLT Q8N122 S771-p Phosphorylation SASSTLGsPENEEHI Q8N122 S792-p Phosphorylation DKMRRASsYSsLNSL Q8N122 S795-p Phosphorylation RRASsYSsLNSLIGV Q8N122 T853-p Phosphorylation RPQRVLDtssLtQsA Q8N122 S854-p Phosphorylation PQRVLDtssLtQsAP Q8N122 S855-p Phosphorylation QRVLDtssLtQsAPA Q8N122 T857-p Phosphorylation VLDtssLtQsAPAsP Q8N122 S859-p Phosphorylation DtssLtQsAPAsPtN Q8N122 S863-p Phosphorylation LtQsAPAsPtNKGVH Q8N122 T865-p Phosphorylation QsAPAsPtNKGVHIH Q8N122 S877-p Phosphorylation HIHQAGGsPPAssts Q8N122 S881-p Phosphorylation AGGsPPAsstsSssL Q8N122 S882-p Phosphorylation GGsPPAsstsSssLt Q8N122 T883-p Phosphorylation GsPPAsstsSssLtN Q8N122 S884-p Phosphorylation sPPAsstsSssLtND Q8N122 S886-p Phosphorylation PAsstsSssLtNDVA Q8N122 S887-p Phosphorylation AsstsSssLtNDVAK Q8N122 T889-p Phosphorylation stsSssLtNDVAKQP Q8N122 T908-p Phosphorylation LPSGRPGtTGPAGAQ Q8N122 Y916-p Phosphorylation TGPAGAQytPHSHQF Q8N122 T917-p Phosphorylation GPAGAQytPHSHQFP Q8N122 S982-p Phosphorylation PEEHDLEsQIRKERE Q8N122 S1189-p Phosphorylation VAGLGDGsIRVyDRR Q8N122 Y1193-p Phosphorylation GDGsIRVyDRRMALS Q8N122 K108-ub Ubiquitination TIGANLQkQYENWQP Q8N122 K120-ub Ubiquitination WQPRARYkQSLDPTV Q8N122 K131-ub Ubiquitination DPTVDEVkKLCTSLR Q8N122 K335-ub Ubiquitination LPRDLFQkLFRQDLL Q8N122 K603-ub Ubiquitination VRDSAHEkLYSLLSD Q8N122 K973-ub Ubiquitination YFAQPVMkIPEEHDL Q8N122 K1008-ub Ubiquitination QAQQVIQkGITRLDD Q8N122 K1056-ub Ubiquitination WDWEKGEkLDYFHNG Q8N122 K1097-ub Ubiquitination DGAIRVWkNFADLEK

PTM - dbPTM UniProt ID Position Code Type Resources References Q8N122 719 S Phosphorylation Swiss-Prot 1010711 18220336;18669648;18722121 Q8N122 721 S Phosphorylation Swiss-Prot 1010711 18722121;19369195 Q8N122 722 S Phosphorylation Swiss-Prot 1010711 18220336;18439900;18669648;18722121;19369195;19413330 Q8N122 792 S Phosphorylation Swiss-Prot 1010711 18439900 Q8N122 865 T Phosphorylation Swiss-Prot 1010711 18187866 Q8N122 877 S Phosphorylation Swiss-Prot 1010711 18669648;18691976;19369195;19413330;19690332 Q8N122 792 S Phosphorylation PhosphoELM.10011 20019839 Q8N122 855 S Phosphorylation PhosphoELM.10011 18669648;19864431 Q8N122 859 S Phosphorylation PhosphoELM.10011 18669648;17081983 Q8N122 863 S Phosphorylation PhosphoELM.10011 18669648;19864431;16964243;17081983;15302935 Q8N122 877 S Phosphorylation PhosphoELM.10011 18669648;19864431 Q8N122 881 S Phosphorylation PhosphoELM.10011 18669648 Q8N122 696 S Phosphorylation PhosphoELM.10011 19864431;20169205 Q8N122 719 S Phosphorylation PhosphoELM.10011 18669648;18220336;19276368;18722121 Q8N122 722 S Phosphorylation PhosphoELM.10011 18669648;18220336;19276368;18722121 Q8N122 853 T Phosphorylation PhosphoELM.10011 18669648 Q8N122 865 T Phosphorylation PhosphoELM.10011 18669648 Q8N122 883 T Phosphorylation PhosphoELM.10011 18669648 Q8N122 706 T Phosphorylation PhosphoELM.10011 19864431;20169205 Q8N122 723 Y Phosphorylation PhosphoELM.10011 18669648 Q8N122 1008 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8N122 1056 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8N122 108 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8N122 1097 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q8N122 120 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8N122 131 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8N122 335 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q8N122 603 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8N122 973 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8N122 696 S Phosphorylation Phosphositeplus.1010730 19864431;20169205;21071439;21325048;21460630;22493283 Q8N122 704 S Phosphorylation Phosphositeplus.1010730 21325048 Q8N122 711 S Phosphorylation Phosphositeplus.1010730 20169205 Q8N122 719 S Phosphorylation Phosphositeplus.1010730 18220336;18669648;18722121;19276368;20169205;21071439;22115753;22322096 Q8N122 721 S Phosphorylation Phosphositeplus.1010730 18722121;20169205;21071439;21325048 Q8N122 722 S Phosphorylation Phosphositeplus.1010730 18220336;18439900;18669648;18722121;19276368;19369195;20169205;20363803;21071439;21325048;21900751;22115753 Q8N122 738 S Phosphorylation Phosphositeplus.1010730 20169205 Q8N122 771 S Phosphorylation Phosphositeplus.1010730 20169205;21757713 Q8N122 792 S Phosphorylation Phosphositeplus.1010730 18439900;20169205;20227367;20354225;21460630;21900751 Q8N122 795 S Phosphorylation Phosphositeplus.1010730 20169205 Q8N122 8 S Phosphorylation Phosphositeplus.1010730 21071439 Q8N122 854 S Phosphorylation Phosphositeplus.1010730 21325048 Q8N122 855 S Phosphorylation Phosphositeplus.1010730 19864431;20169205;21325048;21460630 Q8N122 859 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;19346248;19369195;19415658;19664995;19690332;19864431;20068231;20169205;20230923;20363803;21071439;21325048;21460630;22115753 Q8N122 863 S Phosphorylation Phosphositeplus.1010730 15302935;16964243;18187866;18491316;18669648;18691976;19276368;19346248;19369195;19415658;19664994;19664995;19690332;19864431;20058876;20068231;20164059;20166139;20169205;20230923;20363803;20562096;20886841;21071439;21325048;21406692;21460630;21659604;21757713;21900751;22115753;22493283 Q8N122 877 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;18722121;19276368;19369195;19690332;19864431;20068231;20169205;20363803;20562096;21071439;21325048;21406692;21460630;21900751 Q8N122 881 S Phosphorylation Phosphositeplus.1010730 20169205;21325048 Q8N122 882 S Phosphorylation Phosphositeplus.1010730 21325048 Q8N122 884 S Phosphorylation Phosphositeplus.1010730 21325048 Q8N122 886 S Phosphorylation Phosphositeplus.1010730 19276368;20169205;21325048 Q8N122 887 S Phosphorylation Phosphositeplus.1010730 21325048 Q8N122 699 T Phosphorylation Phosphositeplus.1010730 21325048 Q8N122 700 T Phosphorylation Phosphositeplus.1010730 21325048 Q8N122 706 T Phosphorylation Phosphositeplus.1010730 19864431;20169205;21071439;21325048;21460630;21900751;22493283 Q8N122 853 T Phosphorylation Phosphositeplus.1010730 18669648;20169205 Q8N122 857 T Phosphorylation Phosphositeplus.1010730 19415658;19664995;20169205;21325048 Q8N122 865 T Phosphorylation Phosphositeplus.1010730 18187866;18669648;19415658;20169205;21325048;22115753 Q8N122 883 T Phosphorylation Phosphositeplus.1010730 20169205;21325048 Q8N122 889 T Phosphorylation Phosphositeplus.1010730 19276368;20169205;21325048 Q8N122 908 T Phosphorylation Phosphositeplus.1010730 22356909 Q8N122 917 T Phosphorylation Phosphositeplus.1010730 22356909 Q8N122 692 Y Phosphorylation Phosphositeplus.1010730 21325048 Q8N122 723 Y Phosphorylation Phosphositeplus.1010730 20169205 Q8N122 719 S Phosphorylation HPRD 9.0 18669648 Q8N122 722 S Phosphorylation HPRD 9.0 18669648,19413330,18767875 Q8N122 855 S Phosphorylation HPRD 9.0 18669648 Q8N122 859 S Phosphorylation HPRD 9.0 17081983,18669648,19413330,19415658,18691976,19664995,19651622,18452278,20230923,20068231 Q8N122 863 S Phosphorylation HPRD 9.0 15302935,18669648,19413330,19415658,18691976,18491316,19664995,19651622,19664994,16964243,18452278,20230923,20166139,20068231,20058876 Q8N122 877 S Phosphorylation HPRD 9.0 18669648,19413330,18691976,19651622,20068231 Q8N122 881 S Phosphorylation HPRD 9.0 18669648 Q8N122 884 S Phosphorylation HPRD 9.0 17081983 Q8N122 853 T Phosphorylation HPRD 9.0 18669648,20068231 Q8N122 857 T Phosphorylation HPRD 9.0 19415658,19664995,20230923,20068231 Q8N122 865 T Phosphorylation HPRD 9.0 18669648,19415658,18767875,20230923,20068231 Q8N122 883 T Phosphorylation HPRD 9.0 18669648 Q8N122 723 Y Phosphorylation HPRD 9.0 18669648 Q8N122 719 S Phosphorylation SysPTM 1.1 18669648 Q8N122 722 S Phosphorylation SysPTM 1.1 18669648 Q8N122 859 S Phosphorylation SysPTM 1.1 18669648;17081983;18691976 Q8N122 863 S Phosphorylation SysPTM 1.1 18669648;17081983;18691976;16964243;18491316 Q8N122 877 S Phosphorylation SysPTM 1.1 18669648;18691976 Q8N122 884 S Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References RPTOR 719 S Phosphorylation 18669648 RPTOR 722 S Phosphorylation 18669648,19413330,18767875 RPTOR 723 Y Phosphorylation 18669648 RPTOR 853 T Phosphorylation 18669648,20068231 RPTOR 855 S Phosphorylation 18669648 RPTOR 857 T Phosphorylation 19415658,19664995,20230923,20068231 RPTOR 859 S Phosphorylation 17081983,18669648,19413330,19415658,18691976,19664995,19651622,18452278,20230923,20068231 RPTOR 863 S Phosphorylation 15302935,18669648,19413330,19415658,18691976,18491316,19664995,19651622,19664994,16964243,18452278,20230923,20166139,20068231,20058876 RPTOR 865 T Phosphorylation 18669648,19415658,18767875,20230923,20068231 RPTOR 877 S Phosphorylation 18669648,19413330,18691976,19651622,20068231 RPTOR 881 S Phosphorylation 18669648 RPTOR 883 T Phosphorylation 18669648 RPTOR 884 S Phosphorylation 17081983

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q8N122 865 T 18669648 HTP Phosphorylation Q8N122 706 T 19864431 LTP Phosphorylation Q8N122 706 T 20169205 CDK1 LTP Phosphorylation Q8N122 877 S 18669648 HTP Phosphorylation Q8N122 877 S 19864431 LTP Phosphorylation Q8N122 792 S 20019839 LTP Phosphorylation Q8N122 719 S 18669648 HTP Phosphorylation Q8N122 719 S 18220336 HTP Phosphorylation Q8N122 719 S 19276368 LTP Phosphorylation Q8N122 719 S 18722121 RSK_group LTP Phosphorylation Q8N122 881 S 18669648 HTP Phosphorylation Q8N122 722 S 18669648 HTP Phosphorylation Q8N122 722 S 18220336 HTP Phosphorylation Q8N122 722 S 19276368 LTP Phosphorylation Q8N122 722 S 18722121 RSK_group LTP Phosphorylation Q8N122 883 T 18669648 HTP Phosphorylation Q8N122 853 T 18669648 HTP Phosphorylation Q8N122 723 Y 18669648 HTP Phosphorylation Q8N122 855 S 18669648 HTP Phosphorylation Q8N122 855 S 19864431 LTP Phosphorylation Q8N122 696 S 19864431 LTP Phosphorylation Q8N122 696 S 20169205 CDK1 LTP Phosphorylation Q8N122 859 S 18669648 HTP Phosphorylation Q8N122 859 S 17081983 HTP Phosphorylation Q8N122 863 S 18669648 HTP Phosphorylation Q8N122 863 S 19864431 LTP Phosphorylation Q8N122 863 S 16964243 HTP Phosphorylation Q8N122 863 S 17081983 HTP Phosphorylation Q8N122 863 S 15302935 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q8N122 696 Phosphoserine; by MAPK8 23186163;19864431;22493283 Q8N122 706 Phosphothreonine; by MAPK8 19864431;22493283 Q8N122 719 Phosphoserine; by RPS6KA1 18669648;23186163;18722121 Q8N122 721 Phosphoserine; by RPS6KA1 18722121 Q8N122 722 Phosphoserine; by AMPK and RPS6KA1 23186163;18439900;18722121 Q8N122 738 Phosphoserine 24275569 Q8N122 792 Phosphoserine; by AMPK 18439900 Q8N122 855 Phosphoserine 19864431 Q8N122 859 Phosphoserine; by MTOR 18669648;19690332;20068231;23186163;19864431 Q8N122 863 Phosphoserine; by MAPK8 and MTOR 16964243;18669648;19690332;20068231;21406692;23186163;19864431;22493283 Q8N122 865 Phosphothreonine 23186163 Q8N122 877 Phosphoserine 18669648;18691976;19369195;19690332;20068231;21406692;23186163;24275569;19864431

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 57521 121582 - K Ubiquitination 21139048 57521 121582 - K Ubiquitination 21890473 57521 121582 - K Ubiquitination 21906983 57521 121582 - K FAT10ylation 22797925 57521 121582 - K Ubiquitination 23297343 57521 121582 - K Ubiquitination 21963094 57521 121582 - K Ubiquitination 25044116 57521 121582 - K Neddylation 18247557

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID RPTOR Homo sapiens (Human) 939; 335 "939,GAIRVWKNFADLE; 335,PRDLFQKLFRQDL" 939:21890473; 335:21890473 RPTOR Homo sapiens (Human) 939; 335 "939,GAIRVWKNFADLE; 335,PRDLFQKLFRQDL" 939:21890473; 335:21890473 RPTOR Homo sapiens (Human) 1097; 973; 1008; 335; 108; 131; 603; 1056; 120 "1097,GAIRVWKNFADLE; 973,FAQPVMKIPEEHD; 1008,AQQVIQKGITRLD; 335,PRDLFQKLFRQDL; 108,IGANLQKQYENWQ; 131,PTVDEVKKLCTSL; 603,RDSAHEKLYSLLS; 1056,DWEKGEKLDYFHN; 120,QPRARYKQSLDPT" "1097:21890473,21906983; 973:21906983; 1008:21906983; 335:21890473,21906983; 108:21906983; 131:21906983; 603:21906983; 1056:21906983; 120:21906983" RPTOR Homo sapiens (Human) 939; 335; 108; 131; 120 "939,GAIRVWKNFADLE; 335,PRDLFQKLFRQDL; 108,IGANLQKQYENWQ; 131,PTVDEVKKLCTSL; 120,QPRARYKQSLDPT" 939:21890473; 335:21890473; 108:21906983; 131:21906983; 120:21906983 RPTOR Homo sapiens (Human) 939; 335 "939,GAIRVWKNFADLE; 335,PRDLFQKLFRQDL" 939:21890473; 335:21890473 RPTOR Homo sapiens (Human) 1097; 335; 108; 131; 120 "1097,GAIRVWKNFADLE; 335,PRDLFQKLFRQDL; 108,IGANLQKQYENWQ; 131,PTVDEVKKLCTSL; 120,QPRARYKQSLDPT" "1097:21890473; 335:21906983,21890473; 108:21906983; 131:21906983; 120:21906983"