PTM - CPLM Gene Name UniProt ID Type Position Code PRKAA2; AMPK; AMPK2 P54646 Acetylation 69 K PRKAA2; AMPK; AMPK2 P54646 Ubiquitination 154 K PRKAA2; AMPK; AMPK2 P54646 Ubiquitination 401 K PRKAA2; AMPK; AMPK2 P54646 Ubiquitination 141 K PRKAA2; AMPK; AMPK2 P54646 Ubiquitination 45 K

PTM - dbPAF Gene Name UniProt ID Position Type Species PRKAA2; AMPK; AMPK2 P54646 161 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 165 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 172 T Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 173 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 176 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 179 Y Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 232 Y Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 324 Y Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 377 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 481 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 483 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 484 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 485 T Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 491 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 500 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 501 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 509 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 511 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 521 T Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 524 T Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 527 S Homo sapiens(Human) PRKAA2; AMPK; AMPK2 P54646 534 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P54646 K69-ac Acetylation KREIQNLkLFRHPHI P54646 R227-m1 Methylation PTLFKKIrGGVFyIP P54646 S161-p Phosphorylation KIADFGLsNMMsDGE P54646 S165-p Phosphorylation FGLsNMMsDGEFLRt P54646 T172-p Phosphorylation sDGEFLRtsCGsPNy P54646 S173-p Phosphorylation DGEFLRtsCGsPNyA P54646 S176-p Phosphorylation FLRtsCGsPNyAAPE P54646 Y179-p Phosphorylation tsCGsPNyAAPEVIS P54646 Y232-p Phosphorylation KIrGGVFyIPEYLNR P54646 Y324-p Phosphorylation QDQLAVAyHLIIDNR P54646 S377-p Phosphorylation MPPLIADsPKARCPL P54646 Y436-p Phosphorylation EWKVVNAyHLRVRRK P54646 S481-p Phosphorylation DEVVEQRsGsStPQR P54646 S483-p Phosphorylation VVEQRsGsStPQRsC P54646 T485-p Phosphorylation EQRsGsStPQRsCsA P54646 S489-p Phosphorylation GsStPQRsCsAAGLH P54646 S491-p Phosphorylation StPQRsCsAAGLHRP P54646 S500-p Phosphorylation AGLHRPRssFDSTTA P54646 S501-p Phosphorylation GLHRPRssFDSTTAE P54646 S509-p Phosphorylation FDSTTAEsHsLSGsL P54646 S511-p Phosphorylation STTAEsHsLSGsLTG P54646 S515-p Phosphorylation EsHsLSGsLTGSLtG P54646 T521-p Phosphorylation GsLTGSLtGStLSsV P54646 T524-p Phosphorylation TGSLtGStLSsVSPR P54646 S527-p Phosphorylation LtGStLSsVSPRLGs P54646 S534-p Phosphorylation sVSPRLGsHTMDFFE P54646 K401-sm Sumoylation SLAVKKAkWHLGIRS P54646 K45-ub Ubiquitination TGHKVAVkILNRQKI

PTM - dbPTM UniProt ID Position Code Type Resources References P54646 69 K Acetylation Swiss-Prot 1010711 19608861 P54646 172 T Phosphorylation Swiss-Prot 1010711 15980064 P54646 173 S Phosphorylation Swiss-Prot 1010711 18220336 P54646 176 S Phosphorylation Swiss-Prot 1010711 18220336;18691976 P54646 377 S Phosphorylation Swiss-Prot 1010711 18669648;18691976;19369195;19413330 P54646 500 S Phosphorylation Swiss-Prot 1010711 19369195 P54646 173 S Phosphorylation PhosphoELM.10011 18669648;18220336 P54646 176 S Phosphorylation PhosphoELM.10011 18220336 P54646 377 S Phosphorylation PhosphoELM.10011 18669648 P54646 172 T Phosphorylation PhosphoELM.10011 10642499 P54646 173 S Phosphorylation Phosphositeplus.1010730 18220336;19276368;22322096 P54646 176 S Phosphorylation Phosphositeplus.1010730 18220336;18691976;19369195;22322096 P54646 377 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;19369195;19415658 P54646 172 T Phosphorylation Phosphositeplus.1010730 12391032;12413941;15312046;15371448;15465812;15967841;16051629;16600998;17459875;17567959;17728241;18056705;18195011;18482975;19276368;19369195;20231899;20538596;21228273;22137581;22304913;22322096;22456184;22561688 P54646 324 Y Phosphorylation Phosphositeplus.1010730 20068231 P54646 176 S Phosphorylation HPRD 9.0 18691976 P54646 377 S Phosphorylation HPRD 9.0 18669648,19413330,19415658,18691976 P54646 500 S Phosphorylation HPRD 9.0 18691976 P54646 172 T Phosphorylation HPRD 9.0 10642499,18691976 P54646 324 Y Phosphorylation HPRD 9.0 20068231 P54646 176 S Phosphorylation SysPTM 1.1 18691976 P54646 377 S Phosphorylation SysPTM 1.1 18691976;18669648

PTM - HPRD Symbol Position Code Type References PRKAA2 172 T Phosphorylation 10642499,18691976 PRKAA2 176 S Phosphorylation 18691976 PRKAA2 324 Y Phosphorylation 20068231 PRKAA2 377 S Phosphorylation 18669648,19413330,19415658,18691976 PRKAA2 500 S Phosphorylation 18691976

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P54646 377 S 18669648 HTP Phosphorylation P54646 172 T 10642499 AMPK_group LTP Phosphorylation P54646 173 S 18669648 HTP Phosphorylation P54646 173 S 18220336 HTP Phosphorylation P54646 176 S 18220336 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P54646 172 Phosphothreonine; by LKB1 and CaMKK2 15980064 P54646 377 Phosphoserine 18691976;19369195;23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type PRKAA2 IPI00307755 S 377 PPLIADpSPKARCP phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 5563 111550 - K Ubiquitination 21139048 5563 111550 - K Ubiquitination 23293026 5563 111550 - K Ubiquitination 21963094 5563 111550 - K Ubiquitination 24816145