PTM - CPLM Gene Name UniProt ID Type Position Code MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 Ubiquitination 602 K MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 Ubiquitination 360 K MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 Ubiquitination 385 K

PTM - dbPAF Gene Name UniProt ID Position Type Species MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 153 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 158 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 159 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 163 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 164 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 169 Y Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 239 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 240 Y Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 248 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 250 Y Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 26 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 283 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 290 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 293 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 294 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 297 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 300 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 302 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 304 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 309 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 310 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 311 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 312 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 314 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 315 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 331 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 337 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 339 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 344 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 347 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 349 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 37 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 432 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 493 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 514 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 516 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 520 S Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 522 T Homo sapiens(Human) MAP3K2; MAPKKK2; MEKK2 Q9Y2U5 524 T Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9Y2U5 K260-me Methylation PIFEKFGkGGtYPRR Q9Y2U5 K260-m1 Methylation PIFEKFGkGGtYPRR Q9Y2U5 S26-p Phosphorylation KASRPALsLQETRKA Q9Y2U5 S109-p Phosphorylation AVELLDRsIHMKSLK Q9Y2U5 S153-p Phosphorylation AERKKRLsIIGPtsR Q9Y2U5 T158-p Phosphorylation RLsIIGPtsRDRssP Q9Y2U5 S159-p Phosphorylation LsIIGPtsRDRssPP Q9Y2U5 S163-p Phosphorylation GPtsRDRssPPPGyI Q9Y2U5 S164-p Phosphorylation PtsRDRssPPPGyIP Q9Y2U5 Y169-p Phosphorylation RssPPPGyIPDELHQ Q9Y2U5 S239-p Phosphorylation SRMPRAQsyPDNHQE Q9Y2U5 Y240-p Phosphorylation RMPRAQsyPDNHQEF Q9Y2U5 S248-p Phosphorylation PDNHQEFsDyDNPIF Q9Y2U5 Y250-p Phosphorylation NHQEFsDyDNPIFEK Q9Y2U5 T263-p Phosphorylation EKFGkGGtYPRRYHV Q9Y2U5 T283-p Phosphorylation EYNDGRKtFPRARRt Q9Y2U5 T290-p Phosphorylation tFPRARRtQGTSLRs Q9Y2U5 S297-p Phosphorylation tQGTSLRsPVsFsPT Q9Y2U5 S300-p Phosphorylation TSLRsPVsFsPTDHS Q9Y2U5 S302-p Phosphorylation LRsPVsFsPTDHSLS Q9Y2U5 S311-p Phosphorylation TDHSLSTssGSsIFT Q9Y2U5 S312-p Phosphorylation DHSLSTssGSsIFTP Q9Y2U5 S315-p Phosphorylation LSTssGSsIFTPEYD Q9Y2U5 S331-p Phosphorylation SRIRRRGsDIDNPtL Q9Y2U5 T337-p Phosphorylation GsDIDNPtLTVMDIs Q9Y2U5 S344-p Phosphorylation tLTVMDIsPPsRsPR Q9Y2U5 S347-p Phosphorylation VMDIsPPsRsPRAPT Q9Y2U5 S349-p Phosphorylation DIsPPsRsPRAPTNW Q9Y2U5 T432-p Phosphorylation LRDPQEKtLSIFMEY Q9Y2U5 S493-p Phosphorylation GANILRDsTGNVKLG Q9Y2U5 S505-p Phosphorylation KLGDFGAsKRLQTIC Q9Y2U5 S514-p Phosphorylation RLQTICLsGtGMKsV Q9Y2U5 T516-p Phosphorylation QTICLsGtGMKsVtG Q9Y2U5 S520-p Phosphorylation LsGtGMKsVtGtPYW Q9Y2U5 T522-p Phosphorylation GtGMKsVtGtPYWMS Q9Y2U5 T524-p Phosphorylation GMKsVtGtPYWMSPE Q9Y2U5 K360-ub Ubiquitination PTNWRLGkLLGQGAF Q9Y2U5 K385-ub Ubiquitination TGRELAVkQVQFDPD Q9Y2U5 K602-ub Ubiquitination KRIFVEAkLRPSADE

PTM - dbPTM UniProt ID Position Code Type Resources References Q9Y2U5 26 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 153 S Phosphorylation Swiss-Prot 1010711 18669648;18691976;19369195 Q9Y2U5 158 T Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 159 S Phosphorylation Swiss-Prot 1010711 18669648 Q9Y2U5 163 S Phosphorylation Swiss-Prot 1010711 18220336;19369195 Q9Y2U5 164 S Phosphorylation Swiss-Prot 1010711 17192257;18088087;18220336;18669648 Q9Y2U5 239 S Phosphorylation Swiss-Prot 1010711 18691976;19369195;19690332 Q9Y2U5 248 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 293 T Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 294 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 297 S Phosphorylation Swiss-Prot 1010711 18220336;19369195 Q9Y2U5 300 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 302 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 309 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 310 T Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 312 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 314 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 315 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 331 S Phosphorylation Swiss-Prot 1010711 18691976;19369195;19690332 Q9Y2U5 337 T Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 344 S Phosphorylation Swiss-Prot 1010711 18691976;19369195;19690332 Q9Y2U5 347 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 349 S Phosphorylation Swiss-Prot 1010711 18691976;19369195 Q9Y2U5 514 S Phosphorylation Swiss-Prot 1010711 19369195 Q9Y2U5 26 S Phosphorylation PhosphoELM.10011 18220336 Q9Y2U5 153 S Phosphorylation PhosphoELM.10011 18669648 Q9Y2U5 159 S Phosphorylation PhosphoELM.10011 18669648 Q9Y2U5 163 S Phosphorylation PhosphoELM.10011 18669648;18220336 Q9Y2U5 164 S Phosphorylation PhosphoELM.10011 18669648;18220336;18088087;17192257 Q9Y2U5 239 S Phosphorylation PhosphoELM.10011 18669648 Q9Y2U5 297 S Phosphorylation PhosphoELM.10011 18220336 Q9Y2U5 300 S Phosphorylation PhosphoELM.10011 18220336 Q9Y2U5 331 S Phosphorylation PhosphoELM.10011 18669648 Q9Y2U5 344 S Phosphorylation PhosphoELM.10011 18669648 Q9Y2U5 347 S Phosphorylation PhosphoELM.10011 18669648 Q9Y2U5 385 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9Y2U5 602 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9Y2U5 153 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;19369195;20044836;20068231;20166139;20815410;22115753 Q9Y2U5 159 S Phosphorylation Phosphositeplus.1010730 18669648;20068231 Q9Y2U5 163 S Phosphorylation Phosphositeplus.1010730 18220336;19276368;19369195;20166139 Q9Y2U5 164 S Phosphorylation Phosphositeplus.1010730 17192257;18088087;18220336;18669648;18707149;19276368;20044836;20815410;21659604 Q9Y2U5 239 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;19276368;19369195;19690332;20044836;20166139;22115753 Q9Y2U5 26 S Phosphorylation Phosphositeplus.1010730 18220336;19369195 Q9Y2U5 297 S Phosphorylation Phosphositeplus.1010730 18220336;19276368;19369195 Q9Y2U5 300 S Phosphorylation Phosphositeplus.1010730 18220336 Q9Y2U5 302 S Phosphorylation Phosphositeplus.1010730 19369195 Q9Y2U5 312 S Phosphorylation Phosphositeplus.1010730 19369195 Q9Y2U5 315 S Phosphorylation Phosphositeplus.1010730 19369195 Q9Y2U5 331 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;19369195;19690332;20044836;20363803 Q9Y2U5 344 S Phosphorylation Phosphositeplus.1010730 18691976;19369195;19690332;20363803 Q9Y2U5 349 S Phosphorylation Phosphositeplus.1010730 18691976;19369195 Q9Y2U5 514 S Phosphorylation Phosphositeplus.1010730 19369195 Q9Y2U5 520 S Phosphorylation Phosphositeplus.1010730 19369195 Q9Y2U5 290 T Phosphorylation Phosphositeplus.1010730 19276368 Q9Y2U5 337 T Phosphorylation Phosphositeplus.1010730 20044836 Q9Y2U5 169 Y Phosphorylation Phosphositeplus.1010730 20393185 Q9Y2U5 153 S Phosphorylation HPRD 9.0 17322306,18669648,18691976,18212344,18767875,20166139,20068231 Q9Y2U5 159 S Phosphorylation HPRD 9.0 18669648,20068231 Q9Y2U5 163 S Phosphorylation HPRD 9.0 18669648,18691976,19651622,20166139,20068231 Q9Y2U5 164 S Phosphorylation HPRD 9.0 18669648,18088087,19651622,18707149,20068231 Q9Y2U5 239 S Phosphorylation HPRD 9.0 18669648,18691976,20166139 Q9Y2U5 311 S Phosphorylation HPRD 9.0 18691976 Q9Y2U5 312 S Phosphorylation HPRD 9.0 18691976 Q9Y2U5 314 S Phosphorylation HPRD 9.0 18691976 Q9Y2U5 315 S Phosphorylation HPRD 9.0 18691976 Q9Y2U5 331 S Phosphorylation HPRD 9.0 18669648,18691976 Q9Y2U5 344 S Phosphorylation HPRD 9.0 18669648,18691976,19651622 Q9Y2U5 347 S Phosphorylation HPRD 9.0 18669648,18691976 Q9Y2U5 349 S Phosphorylation HPRD 9.0 18691976,19651622 Q9Y2U5 158 T Phosphorylation HPRD 9.0 18691976,20068231 Q9Y2U5 310 T Phosphorylation HPRD 9.0 18691976 Q9Y2U5 153 S Phosphorylation SysPTM 1.1 18691976 Q9Y2U5 164 S Phosphorylation SysPTM 1.1 18088087 Q9Y2U5 239 S Phosphorylation SysPTM 1.1 18691976 Q9Y2U5 331 S Phosphorylation SysPTM 1.1 18691976 Q9Y2U5 344 S Phosphorylation SysPTM 1.1 18691976 Q9Y2U5 349 S Phosphorylation SysPTM 1.1 18691976

PTM - HPRD Symbol Position Code Type References MAP3K2 153 S Phosphorylation 17322306,18669648,18691976,18212344,18767875,20166139,20068231 MAP3K2 158 T Phosphorylation 18691976,20068231 MAP3K2 159 S Phosphorylation 18669648,20068231 MAP3K2 163 S Phosphorylation 18669648,18691976,19651622,20166139,20068231 MAP3K2 164 S Phosphorylation 18669648,18088087,19651622,18707149,20068231 MAP3K2 239 S Phosphorylation 18669648,18691976,20166139 MAP3K2 310 T Phosphorylation 18691976 MAP3K2 311 S Phosphorylation 18691976 MAP3K2 312 S Phosphorylation 18691976 MAP3K2 314 S Phosphorylation 18691976 MAP3K2 315 S Phosphorylation 18691976 MAP3K2 331 S Phosphorylation 18669648,18691976 MAP3K2 344 S Phosphorylation 18669648,18691976,19651622 MAP3K2 347 S Phosphorylation 18669648,18691976 MAP3K2 349 S Phosphorylation 18691976,19651622

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9Y2U5 159 S 18669648 HTP Phosphorylation Q9Y2U5 163 S 18669648 HTP Phosphorylation Q9Y2U5 163 S 18220336 HTP Phosphorylation Q9Y2U5 347 S 18669648 HTP Phosphorylation Q9Y2U5 331 S 18669648 HTP Phosphorylation Q9Y2U5 297 S 18220336 HTP Phosphorylation Q9Y2U5 164 S 18669648 HTP Phosphorylation Q9Y2U5 164 S 18220336 HTP Phosphorylation Q9Y2U5 164 S 18088087 HTP Phosphorylation Q9Y2U5 164 S 17192257 HTP Phosphorylation Q9Y2U5 26 S 18220336 HTP Phosphorylation Q9Y2U5 300 S 18220336 HTP Phosphorylation Q9Y2U5 153 S 18669648 HTP Phosphorylation Q9Y2U5 344 S 18669648 HTP Phosphorylation Q9Y2U5 239 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9Y2U5 26 Phosphoserine 19369195 Q9Y2U5 153 Phosphoserine 18669648;18691976;19369195;20068231;23186163 Q9Y2U5 159 Phosphoserine 18669648 Q9Y2U5 164 Phosphoserine 18669648 Q9Y2U5 239 Phosphoserine 19690332;23186163 Q9Y2U5 297 Phosphoserine 18220336 Q9Y2U5 311 Phosphoserine 23186163 Q9Y2U5 331 Phosphoserine 18691976;19369195;19690332;23186163;24275569 Q9Y2U5 344 Phosphoserine 18691976;19369195;19690332

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type MAP3K2 IPI00513803 S 153 ERKKRLpSIIGPTS phosphorylation MAP3K2 IPI00513803 S 159 SIIGPTpSRDRSSP phosphorylation MAP3K2 IPI00513803 S 163 PTSRDRpSSPPPGY phosphorylation MAP3K2 IPI00513803 S 164 TSRDRSpSPPPGYI phosphorylation MAP3K2 IPI00513803 S 239 RMPRAQpSYPDNHQ phosphorylation MAP3K2 IPI00513803 S 26 ASRPALpSLQETRK phosphorylation MAP3K2 IPI00513803 S 297 QGTSLRpSPVSFSP phosphorylation MAP3K2 IPI00513803 S 302 RSPVSFpSPTDHSL phosphorylation MAP3K2 IPI00513803 S 312 HSLSTSpSGSSIFT phosphorylation MAP3K2 IPI00513803 S 315 STSSGSpSIFTPEY phosphorylation MAP3K2 IPI00513803 S 331 RIRRRGpSDIDNPT phosphorylation MAP3K2 IPI00513803 S 344 LTVMDIpSPPSRSP phosphorylation MAP3K2 IPI00513803 S 349 ISPPSRpSPRAPTN phosphorylation MAP3K2 IPI00513803 S 514 LQTICLpSGTGMKS phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 10746 115969 - K Ubiquitination 20588253 10746 115969 - K Ubiquitination 21139048 10746 115969 - K Ubiquitination 21890473 10746 115969 - K Ubiquitination 21906983 10746 115969 - K Ubiquitination 22505724 10746 115969 - K Ubiquitination 23000965 10746 115969 - K Ubiquitination 24816145 10746 115969 - K Ubiquitination 24975362

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID MAP3K2 Homo sapiens (Human) 385; 602 "385,GRELAVKQVQFDP; 602,RIFVEAKLRPSAD" 385:21906983; 602:21890473 MAP3K2 Homo sapiens (Human) 146 "146,RIFVEAKLRPSAD" 146:21890473 MAP3K2 Homo sapiens (Human) 602 "602,RIFVEAKLRPSAD" 602:21890473