PTM - CPLM Gene Name UniProt ID Type Position Code UBE2O; KIAA1734 Q9C0C9 Ubiquitination 132 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 825 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 283 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 521 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 620 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 815 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 819 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 866 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 990 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 809 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 859 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 373 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 360 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 1154 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 672 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 852 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 865 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 1248 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 1263 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 1267 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 593 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 365 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 846 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 878 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 1233 K UBE2O; KIAA1734 Q9C0C9 Ubiquitination 1235 K

PTM - dbPAF Gene Name UniProt ID Position Type Species UBE2O; KIAA1734 Q9C0C9 102 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 115 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1166 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1168 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1178 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1196 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1199 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1220 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1227 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1231 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1240 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1241 Y Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 1243 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 180 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 185 Y Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 189 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 322 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 381 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 399 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 401 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 404 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 407 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 411 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 423 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 429 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 431 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 435 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 439 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 441 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 475 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 488 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 491 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 50 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 515 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 519 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 643 Y Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 73 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 782 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 823 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 828 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 834 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 836 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 838 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 839 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 841 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 87 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 89 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 896 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 906 T Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 98 S Homo sapiens(Human) UBE2O; KIAA1734 Q9C0C9 99 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9C0C9 R981-m1 Methylation MVKTFEDrMDLFSAL Q9C0C9 R1242-m1 Methylation KKRRKsyrsFLPEkS Q9C0C9 S50-p Phosphorylation ASGPSSDsGPEAGSQ Q9C0C9 S73-p Phosphorylation VSGRYRGsVHFGLVR Q9C0C9 S87-p Phosphorylation RLIHGEDsDsEGEEE Q9C0C9 S89-p Phosphorylation IHGEDsDsEGEEEGR Q9C0C9 S98-p Phosphorylation GEEEGRGssGCsEAG Q9C0C9 S99-p Phosphorylation EEEGRGssGCsEAGG Q9C0C9 S102-p Phosphorylation GRGssGCsEAGGAGH Q9C0C9 S115-p Phosphorylation GHEEGRAsPLRRGYV Q9C0C9 T180-p Phosphorylation CAVKLIGtNCIIyPV Q9C0C9 Y185-p Phosphorylation IGtNCIIyPVNsKDL Q9C0C9 S189-p Phosphorylation CIIyPVNsKDLQHIW Q9C0C9 S381-p Phosphorylation NCAQGEGsMAKKVKR Q9C0C9 S399-p Phosphorylation KQVVRIMsCsPDtQC Q9C0C9 S401-p Phosphorylation VVRIMsCsPDtQCsR Q9C0C9 T404-p Phosphorylation IMsCsPDtQCsRDHs Q9C0C9 S407-p Phosphorylation CsPDtQCsRDHsMED Q9C0C9 S411-p Phosphorylation tQCsRDHsMEDPDKK Q9C0C9 S431-p Phosphorylation KSEAESAsPEEtPdG Q9C0C9 T435-p Phosphorylation ESAsPEEtPdGSAsP Q9C0C9 S441-p Phosphorylation EtPdGSAsPVEMQDE Q9C0C9 T488-p Phosphorylation DDEAADDtDDtSSVT Q9C0C9 T491-p Phosphorylation AADDtDDtSSVTSSA Q9C0C9 S515-p Phosphorylation SGTSRKKsIPLsIkN Q9C0C9 S519-p Phosphorylation RKKsIPLsIkNLKRK Q9C0C9 Y643-p Phosphorylation EEEDVSVyDIADHPD Q9C0C9 T782-p Phosphorylation VISEEAAtAAVQGAV Q9C0C9 S823-p Phosphorylation EAIkILEsLkNMtVE Q9C0C9 T828-p Phosphorylation LEsLkNMtVEQLLTG Q9C0C9 S836-p Phosphorylation VEQLLTGsPtsPtVE Q9C0C9 T838-p Phosphorylation QLLTGsPtsPtVEPE Q9C0C9 S839-p Phosphorylation LLTGsPtsPtVEPEk Q9C0C9 T841-p Phosphorylation TGsPtsPtVEPEkPT Q9C0C9 S896-p Phosphorylation EDKPEGQsPVKAEWP Q9C0C9 T906-p Phosphorylation KAEWPSEtPVLCQQC Q9C0C9 S1196-p Phosphorylation GPAPGEAsQGsDSEG Q9C0C9 S1199-p Phosphorylation PGEAsQGsDSEGGAQ Q9C0C9 S1220-p Phosphorylation RDHTDQTsETAPdAs Q9C0C9 S1227-p Phosphorylation sETAPdAsVPPsVKP Q9C0C9 S1231-p Phosphorylation PdAsVPPsVKPKKRR Q9C0C9 S1240-p Phosphorylation KPKKRRKsyrsFLPE Q9C0C9 Y1241-p Phosphorylation PKKRRKsyrsFLPEk Q9C0C9 S1243-p Phosphorylation KRRKsyrsFLPEkSG Q9C0C9 K132-ub Ubiquitination QWYPEGVkQHVKETK Q9C0C9 K283-ub Ubiquitination VQWLSGVkPVLSTKS Q9C0C9 K360-ub Ubiquitination CLYVFPAkVEPAkIA Q9C0C9 K365-ub Ubiquitination PAkVEPAkIAWECPE Q9C0C9 K521-ub Ubiquitination KsIPLsIkNLKRKHK Q9C0C9 K593-ub Ubiquitination PGDFVVDkRVQSCPD Q9C0C9 K620-ub Ubiquitination IGRTCMVkWFKLRPS Q9C0C9 K672-ub Ubiquitination TEDGAPHkEDEPSVG Q9C0C9 K809-ub Ubiquitination AGKDGPPkSFRELkE Q9C0C9 K815-ub Ubiquitination PkSFRELkEAIkILE Q9C0C9 K819-ub Ubiquitination RELkEAIkILEsLkN Q9C0C9 K825-ub Ubiquitination IkILEsLkNMtVEQL Q9C0C9 K846-ub Ubiquitination sPtVEPEkPTREKKF Q9C0C9 K866-ub Ubiquitination KLQENLKkTLDNVAI Q9C0C9 K878-ub Ubiquitination VAIVEEEkMEAVPDV Q9C0C9 K990-ub Ubiquitination DLFSALIkGPTRTPY Q9C0C9 K1038-ub Ubiquitination PNLYDNGkVCVSLLG Q9C0C9 K1248-ub Ubiquitination yrsFLPEkSGYPDIG Q9C0C9 K1263-ub Ubiquitination FPLFPLSkGFIkSIR Q9C0C9 K1267-ub Ubiquitination PLSkGFIkSIRGVLT

PTM - dbPTM UniProt ID Position Code Type Resources References Q9C0C9 87 S Phosphorylation Swiss-Prot 1010711 18669648;19413330 Q9C0C9 89 S Phosphorylation Swiss-Prot 1010711 18669648;19413330 Q9C0C9 399 S Phosphorylation Swiss-Prot 1010711 18669648;19413330 Q9C0C9 401 S Phosphorylation Swiss-Prot 1010711 18669648;19413330 Q9C0C9 435 T Phosphorylation Swiss-Prot 1010711 18669648 Q9C0C9 441 S Phosphorylation Swiss-Prot 1010711 18669648 Q9C0C9 836 S Phosphorylation Swiss-Prot 1010711 18669648;19413330;19690332 Q9C0C9 838 T Phosphorylation Swiss-Prot 1010711 18220336;18669648;19413330 Q9C0C9 839 S Phosphorylation Swiss-Prot 1010711 18220336;19413330 Q9C0C9 841 T Phosphorylation Swiss-Prot 1010711 18220336 Q9C0C9 896 S Phosphorylation Swiss-Prot 1010711 17287340 Q9C0C9 896 S Phosphorylation PhosphoELM.10011 17287340 Q9C0C9 1225 D Caspase Phosphositeplus.1010730 18722006 Q9C0C9 437 D Caspase Phosphositeplus.1010730 18724940 Q9C0C9 1248 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9C0C9 132 K Ubiquitylation Phosphositeplus.1010730 20639865;21890473;21906983 Q9C0C9 283 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9C0C9 521 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9C0C9 620 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9C0C9 672 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9C0C9 815 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9C0C9 819 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9C0C9 825 K Ubiquitylation Phosphositeplus.1010730 20639865;21890473;21906983 Q9C0C9 846 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9C0C9 866 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9C0C9 878 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9C0C9 990 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9C0C9 102 S Phosphorylation Phosphositeplus.1010730 21659604 Q9C0C9 1240 S Phosphorylation Phosphositeplus.1010730 21406692 Q9C0C9 189 S Phosphorylation Phosphositeplus.1010730 20068231 Q9C0C9 399 S Phosphorylation Phosphositeplus.1010730 18669648 Q9C0C9 401 S Phosphorylation Phosphositeplus.1010730 18669648;19664994;19664995;22115753 Q9C0C9 441 S Phosphorylation Phosphositeplus.1010730 18669648;22115753 Q9C0C9 515 S Phosphorylation Phosphositeplus.1010730 20363803 Q9C0C9 836 S Phosphorylation Phosphositeplus.1010730 18669648;18847512;19276368;19690332;20363803;21406692;21659604;22115753 Q9C0C9 839 S Phosphorylation Phosphositeplus.1010730 18220336;18669648;19276368;19415658;20363803;20562096 Q9C0C9 87 S Phosphorylation Phosphositeplus.1010730 18669648;19690332;20068231;20164059;20363803;21406692;21659604 Q9C0C9 89 S Phosphorylation Phosphositeplus.1010730 18669648;19690332;20068231;20164059;20363803;21406692;21659604 Q9C0C9 896 S Phosphorylation Phosphositeplus.1010730 17287340;19276368;20068231;20164059;20363803;20562096;21406692;22115753 Q9C0C9 98 S Phosphorylation Phosphositeplus.1010730 21659604 Q9C0C9 99 S Phosphorylation Phosphositeplus.1010730 21659604 Q9C0C9 180 T Phosphorylation Phosphositeplus.1010730 20068231 Q9C0C9 838 T Phosphorylation Phosphositeplus.1010730 18220336;20562096;20815410;21659604 Q9C0C9 841 T Phosphorylation Phosphositeplus.1010730 18220336;19276368 Q9C0C9 185 Y Phosphorylation Phosphositeplus.1010730 20068231 Q9C0C9 87 S Phosphorylation HPRD 9.0 18669648,19413330,19651622,20068231 Q9C0C9 89 S Phosphorylation HPRD 9.0 18669648,19413330,19651622,20068231 Q9C0C9 189 S Phosphorylation HPRD 9.0 20068231 Q9C0C9 399 S Phosphorylation HPRD 9.0 18669648,19413330 Q9C0C9 401 S Phosphorylation HPRD 9.0 18669648,19413330,19664995,19664994 Q9C0C9 429 S Phosphorylation HPRD 9.0 18669648 Q9C0C9 431 S Phosphorylation HPRD 9.0 18669648 Q9C0C9 441 S Phosphorylation HPRD 9.0 18669648 Q9C0C9 836 S Phosphorylation HPRD 9.0 18669648,19413330,19651622,20068231 Q9C0C9 839 S Phosphorylation HPRD 9.0 18669648,19413330,20068231 Q9C0C9 896 S Phosphorylation HPRD 9.0 17287340,19651622,20068231 Q9C0C9 180 T Phosphorylation HPRD 9.0 20068231 Q9C0C9 423 T Phosphorylation HPRD 9.0 18669648 Q9C0C9 435 T Phosphorylation HPRD 9.0 18669648 Q9C0C9 834 T Phosphorylation HPRD 9.0 20068231 Q9C0C9 838 T Phosphorylation HPRD 9.0 18669648,19413330,19651622,20068231 Q9C0C9 841 T Phosphorylation HPRD 9.0 18669648,20068231 Q9C0C9 185 Y Phosphorylation HPRD 9.0 20068231 Q9C0C9 836 S Phosphorylation SysPTM 1.1 18669648 Q9C0C9 838 T Phosphorylation SysPTM 1.1 18669648

PTM - HPRD Symbol Position Code Type References UBE2O 180 T Phosphorylation 20068231 UBE2O 185 Y Phosphorylation 20068231 UBE2O 189 S Phosphorylation 20068231 UBE2O 399 S Phosphorylation 18669648,19413330 UBE2O 401 S Phosphorylation 18669648,19413330,19664995,19664994 UBE2O 423 T Phosphorylation 18669648 UBE2O 429 S Phosphorylation 18669648 UBE2O 431 S Phosphorylation 18669648 UBE2O 435 T Phosphorylation 18669648 UBE2O 441 S Phosphorylation 18669648 UBE2O 834 T Phosphorylation 20068231 UBE2O 836 S Phosphorylation 18669648,19413330,19651622,20068231 UBE2O 838 T Phosphorylation 18669648,19413330,19651622,20068231 UBE2O 839 S Phosphorylation 18669648,19413330,20068231 UBE2O 841 T Phosphorylation 18669648,20068231 UBE2O 87 S Phosphorylation 18669648,19413330,19651622,20068231 UBE2O 89 S Phosphorylation 18669648,19413330,19651622,20068231 UBE2O 896 S Phosphorylation 17287340,19651622,20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9C0C9 896 S 17287340 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9C0C9 50 Phosphoserine 24275569 Q9C0C9 87 Phosphoserine 19690332;20068231;21406692;23186163;24275569 Q9C0C9 89 Phosphoserine 20068231;21406692;23186163;24275569 Q9C0C9 399 Phosphoserine 18669648;23186163 Q9C0C9 401 Phosphoserine 18669648;23186163 Q9C0C9 441 Phosphoserine 18669648 Q9C0C9 488 Phosphothreonine 24275569 Q9C0C9 491 Phosphothreonine 24275569 Q9C0C9 515 Phosphoserine 23186163 Q9C0C9 836 Phosphoserine 18669648;19690332;23186163 Q9C0C9 896 Phosphoserine 21406692;23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type UBE2O IPI00028307 S 836 EQLLTGpSPTSPTV phosphorylation UBE2O IPI00028307 S 839 LTGSPTpSPTVEPE phosphorylation UBE2O IPI00028307 S 87 LIHGEDpSDSEGEE phosphorylation UBE2O IPI00028307 S 89 HGEDSDpSEGEEEG phosphorylation UBE2O IPI00028307 T 838 LLTGSPpTSPTVEP phosphorylation UBE2O IPI00028307 T 841 GSPTSPpTVEPEKP phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 63893 121973 - K Ubiquitination 20639865 63893 121973 - K Ubiquitination 21139048 63893 121973 - K Ubiquitination 21890473 63893 121973 - K Ubiquitination 21906983 63893 121973 - K Ubiquitination 22053931 63893 121973 - K Ubiquitination 22505724 63893 121973 - K Ubiquitination 21963094 63893 121973 - K Ubiquitination 23000965 63893 121973 - K Ubiquitination 24703950 63893 121973 - K Ubiquitination 24816145 63893 121973 - K Ubiquitination 25015289 63893 121973 - K Ubiquitination 12938929

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID UBE2O Homo sapiens (Human) 593 "593,GDFVVDKRVQSCP" 593:22053931 UBE2O Homo sapiens (Human) 866; 990; 819; 1248; 283; 815; 825; 846; 132; 521; 620; 878; 672 "866,LQENLKKTLDNVA; 990,LFSALIKGPTRTP; 819,ELKEAIKILESLK; 1248,RSFLPEKSGYPDI; 283,QWLSGVKPVLSTK; 815,KSFRELKEAIKIL; 825,KILESLKNMTVEQ; 846,PTVEPEKPTREKK; 132,WYPEGVKQHVKET; 521,SIPLSIKNLKRKH; 620,GRTCMVKWFKLRP; 878,AIVEEEKMEAVPD; 672,EDGAPHKEDEPSV" "866:21890473,21906983; 990:21890473,21906983; 819:21890473; 1248:21906983; 283:21890473; 815:21890473; 825:21890473,21906983; 846:21906983; 132:21890473,21906983; 521:21890473; 620:21890473; 878:21906983; 672:21906983"