PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus Ywhae Trim25 16615898 intact exp GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Hsd3b7 16615898 intact exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ubc Trim25 23649778 biogrid exp GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Pgls 16615898 intact exp GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Eed 24457600 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Sfn Trim25 12075357 mint,intact exp, ortho P G G G G G G G G Trim25 Ddx58 24015671,23209422 biogrid exp, ortho P Rab8a Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Protein Ddx19b ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Ube2d2b ortho P G Trim25 Med4 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Smchd1 ortho P Pfkm Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Pitx2 Trim25 ortho G G G G G G G G G G G G Gata1 Trim25 ortho G G G G G G G Trim25 Rbck1 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Sec31a Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Ube2j2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Vcp Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Lax1 ortho Eef1a2 Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Otub2 ortho G G G G G G G G G G G Trim25 Commd4 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Wars Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Hspa1l Trim25 ortho P G G G Trim25 Rnf31 ortho P Trim25 Ubxn6 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Trim8 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Pax2 Trim25 ortho Trim25 Ube2q2 ortho Trim25 Picalm ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Ube2s ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Grb7 Trim25 ortho GP G G G G G G G G G G G G G G G G G G Jun Trim25 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Znf24 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Cda Trim25 ortho G G G G G G G G G G G G G G G G G Uba1 Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Uros Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Ube2l6 ortho G G G G G G G G G Uncharacterized protein C9orf78 homolog Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Esr1 Trim25 ortho G G G G G Trim25 Fbxw7 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Rnf2 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp39 Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Zfhx3 Trim25 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Txnrd1 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Tceal1 ortho G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Scly ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G Rps27a Trim25 ortho P Apc Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Usp15 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Protein Tfg, TFG protein, Trk-fused, Trk-fused protein ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Pcna Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Rnpep ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Grik2 Trim25 ortho G G G Trim25 Ptprj ortho GP G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Acly ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Hecw2 ortho Ezh2 Trim25 ortho G G G G G G G G G G G G G G G G G G G G G G G Trim25 UPF0505 protein C16orf62 homolog ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ube2n Trim25 ortho P Ctla4 Trim25 ortho G G Bmi1 Trim25 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Ccdc22 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Klf5 ortho G G G G G G G G G G G G G G G G G G G G G G G Sirt6 Trim25 ortho Trim25 Amfr ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Traf6 Trim25 ortho G G G G G G G G G G G G G G G G G G G G Trim25 Rpa1 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Rara Trim25 ortho G G Trim25 UPF0568 protein C14orf166 homolog ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Xrcc3 ortho Eef1a1 Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Isg15 ortho P Sars Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Igf1r Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Lix1l ortho Ube2d2 Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ccdc8 Trim25 ortho Meis2 Trim25 ortho G G G G G G G G G G G G G G G G G G G G Trim25 Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ube2d3 Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Tsg101 Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Cul7 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Tpd52l2 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Uap1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ercc2 Trim25 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Fam98b ortho P Ybx1 Trim25 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Suz12 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Kif5b ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ube2d1 Trim25 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Med20 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Stk38 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim25 Stk11 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q61510 O95786 enzymatic study 23209422 direct interaction X 2 Q61510 O95786 affinity chromatography technology 20818395 physical association X 2 Q61510 Q13501 affinity chromatography technology 23649778 physical association X 2 Q61510 P54787 affinity chromatography technology 23649778 physical association X 2 Q61510 Q921E6 affinity chromatography technology 24457600 physical association X 2 P48510 Q61510 affinity chromatography technology 23649778 physical association X 2 Q61510 P54727 affinity chromatography technology 23649778 physical association X 2 Q61510 A0A024R1V3 affinity chromatography technology 23649778 physical association X 2 Q61510 affinity chromatography technology 23649778 physical association X 2 Q61510 A0A024R284 affinity chromatography technology 23649778 physical association X 2 P55036 Q61510 affinity chromatography technology 23649778 physical association X 2 Q6Q899 Q61510 enzymatic study 23209422 direct interaction X 2 Q6Q899 Q61510 affinity chromatography technology 24015671 physical association X 2 Q61510 cosedimentation 16615898 colocalization C 1216 O14933 Q61510 coimmunoprecipitation 12075357 physical association X 2 O70456 Q61510 coimmunoprecipitation 12075357 physical association X 2 O70456 Q61510 experimental interaction detection 12075357 ubiquitination reaction X 2 O70456 Q61510 fluorescence microscopy 12075357 colocalization X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B Sfn Trim25 MI:0019(coimmunoprecipitation)|MI:0019(coimmunoprecipitation)|MI:0019(coimmunoprecipitation)|MI:0019(coimmunoprecipitation)|MI:0019(coimmunoprecipitation)|MI:0019(coimmunoprecipitation)|MI:0019(coimmunoprecipitation) 12075357 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0220(ubiquitination reaction)|MI:0915(physical association)|MI:0915(physical association)|MI:0403(colocalization)|MI:0915(physical association)|MI:0403(colocalization)|MI:0220(ubiquitination reaction) MI:0471(mint)|MI:0471(mint)|(intact)|MI:0471(mint)|(intact)|(intact)|(intact) MINT_86087|MINT_85412|UniProt_71233|MINT_85411|UniProt_163680|UniProt_163683|UniProt_163682 unspecified role|prey|prey|unspecified role|prey|neutral component|unspecified role unspecified role|bait|bait|neutral component|bait|neutral component|unspecified role Trim25 Eed MI:0004(affinity chromatography technology) 24457600 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_332470 prey bait Trim25 Ddx58 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 23209422,24015671 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(biogrid) BIOGRID_325046|BIOGRID_331605 bait|bait prey|prey

PPI - HINT Gene A Gene B PMID Method Quality SFN TRIM25 12075357,12075357,12075357 0416,0045,0019 LC YWHAE TRIM25 16615898 0027 LC TRIM25 TRIM25 24015671 0114 LC TRIM25 PGLS 16615898 0027 LC TRIM25 HSD3B7 16615898 0027 LC UBC TRIM25 23649778 0004 LC TRIM25 DDX58 23209422,24015671 0004,0004 LC TRIM25 EED 24457600 0004 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q61510 O14933 TRIM25 UBE2L6 0.21 12075357 Q6Q899 Q61510 DDX58 TRIM25 0.376 24015671,23209422 Q61510 P62259 TRIM25 YWHAE 0.081 16615898 Q61510 P0CG50 TRIM25 UBC 0.126 23649778 Q61510 O95786 TRIM25 DDX58 0.21 20818395 Q61510 O70456 TRIM25 SFN 0.332 12075357 Q921E6 Q61510 EED TRIM25 0.126 24457600 Q9CQ60 Q61510 PGLS TRIM25 0.081 16615898 Q9EQC1 Q61510 HSD3B7 TRIM25 0.081 16615898