PTM - CPLM Gene Name UniProt ID Type Position Code Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 127 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 212 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 516 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 588 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 667 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 812 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 816 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 822 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 843 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 849 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 863 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 940 K Ube2o; Kiaa1734 Q6ZPJ3 Ubiquitination 987 K

PTM - dbPAF Gene Name UniProt ID Position Type Species Ube2o; Kiaa1734 Q6ZPJ3 110 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 1110 T Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 1175 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 1177 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 1193 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 1196 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 1198 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 1227 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 1236 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 1239 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 269 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 394 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 406 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 424 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 426 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 43 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 436 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 445 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 470 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 483 T Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 486 T Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 487 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 488 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 494 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 501 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 510 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 514 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 638 Y Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 82 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 831 T Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 833 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 835 T Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 836 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 838 T Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 84 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 845 T Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 893 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 93 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 94 S Mus musculus(Mouse) Ube2o; Kiaa1734 Q6ZPJ3 97 S Mus musculus(Mouse)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q6ZPJ3 S68-p Phosphorylation VSGRYRGsVHFGLVR Q6ZPJ3 S82-p Phosphorylation RLIHGEDsDsEGDDD Q6ZPJ3 S84-p Phosphorylation IHGEDsDsEGDDDGR Q6ZPJ3 S93-p Phosphorylation GDDDGRGssGCsEAG Q6ZPJ3 S94-p Phosphorylation DDDGRGssGCsEAGG Q6ZPJ3 S97-p Phosphorylation GRGssGCsEAGGAGH Q6ZPJ3 S110-p Phosphorylation GHEEGRAsPLRRGYV Q6ZPJ3 S269-p Phosphorylation IGPAKIFsSVQWLSG Q6ZPJ3 S394-p Phosphorylation KQVVRIMsCTPDTQC Q6ZPJ3 S406-p Phosphorylation TQCPRDHsMEDPDKK Q6ZPJ3 S426-p Phosphorylation GSEIGSAsPEEQPDG Q6ZPJ3 S436-p Phosphorylation EQPDGSAsPVEMQDE Q6ZPJ3 S470-p Phosphorylation GGDDGLHsAEQDADD Q6ZPJ3 T483-p Phosphorylation DDEAADDtDDtssVT Q6ZPJ3 T486-p Phosphorylation AADDtDDtssVTSSA Q6ZPJ3 S487-p Phosphorylation ADDtDDtssVTSSAs Q6ZPJ3 S488-p Phosphorylation DDtDDtssVTSSAsS Q6ZPJ3 S494-p Phosphorylation ssVTSSAsSTTSSQs Q6ZPJ3 S501-p Phosphorylation sSTTSSQsGSGTGRK Q6ZPJ3 S510-p Phosphorylation SGTGRKKsIPLsIkN Q6ZPJ3 S514-p Phosphorylation RKKsIPLsIkNLKRK Q6ZPJ3 Y638-p Phosphorylation EEEDVSVyDIADHPD Q6ZPJ3 T825-p Phosphorylation LESLkNMtVEQLLTG Q6ZPJ3 S833-p Phosphorylation VEQLLTGsPtsPtVE Q6ZPJ3 T835-p Phosphorylation QLLTGsPtsPtVEPE Q6ZPJ3 S836-p Phosphorylation LLTGsPtsPtVEPEk Q6ZPJ3 T838-p Phosphorylation TGsPtsPtVEPEkPT Q6ZPJ3 S893-p Phosphorylation EEKPEVQsPVKAEWP Q6ZPJ3 T1110-p Phosphorylation IRVVQSMtQLVRRPP Q6ZPJ3 S1193-p Phosphorylation GMAPGEAsQGsDsEG Q6ZPJ3 S1196-p Phosphorylation PGEAsQGsDsEGGAQ Q6ZPJ3 S1198-p Phosphorylation EAsQGsDsEGGAQGP Q6ZPJ3 S1227-p Phosphorylation PDASAPPsVRPKRRR Q6ZPJ3 S1236-p Phosphorylation RPKRRRKsYRSFLPE Q6ZPJ3 K127-ub Ubiquitination QWYPEGVkQHVkETK Q6ZPJ3 K131-ub Ubiquitination EGVkQHVkETKLKLE Q6ZPJ3 K171-ub Ubiquitination VNIDCAVkLIGTNCI Q6ZPJ3 K212-ub Ubiquitination LGKVYDLkNQIILKL Q6ZPJ3 K516-ub Ubiquitination KsIPLsIkNLKRKHK Q6ZPJ3 K588-ub Ubiquitination PGDFVVDkRVQSCPD Q6ZPJ3 K667-ub Ubiquitination TEDGALPkEDEPSVG Q6ZPJ3 K812-ub Ubiquitination PKSFRELkEAIkILE Q6ZPJ3 K816-ub Ubiquitination RELkEAIkILESLkN Q6ZPJ3 K822-ub Ubiquitination IkILESLkNMtVEQL Q6ZPJ3 K843-ub Ubiquitination sPtVEPEkPTREKkF Q6ZPJ3 K849-ub Ubiquitination EkPTREKkFLDDIKK Q6ZPJ3 K863-ub Ubiquitination KLQENLKkTLDNVAI Q6ZPJ3 K940-ub Ubiquitination PSNHSFKkIEFQPPE Q6ZPJ3 K987-ub Ubiquitination DLFSALIkGPTRTPY Q6ZPJ3 K1259-ub Ubiquitination FPLFPLSkGFIKSIR

PTM - dbPTM UniProt ID Position Code Type Resources References Q6ZPJ3 82 S Phosphorylation Swiss-Prot 1010711 17242355;19367708 Q6ZPJ3 84 S Phosphorylation Swiss-Prot 1010711 17242355;19367708 Q6ZPJ3 833 S Phosphorylation Swiss-Prot 1010711 17242355 Q6ZPJ3 836 S Phosphorylation Swiss-Prot 1010711 17242355 Q6ZPJ3 893 S Phosphorylation Swiss-Prot 1010711 19367708 Q6ZPJ3 82 S Phosphorylation PhosphoELM.10011 17242355 Q6ZPJ3 84 S Phosphorylation PhosphoELM.10011 17242355 Q6ZPJ3 833 S Phosphorylation PhosphoELM.10011 17242355 Q6ZPJ3 836 S Phosphorylation PhosphoELM.10011 17242355 Q6ZPJ3 110 S Phosphorylation Phosphositeplus.1010730 21183079 Q6ZPJ3 1193 S Phosphorylation Phosphositeplus.1010730 21183079;21659605 Q6ZPJ3 1196 S Phosphorylation Phosphositeplus.1010730 21183079;21659605 Q6ZPJ3 1198 S Phosphorylation Phosphositeplus.1010730 21183079 Q6ZPJ3 394 S Phosphorylation Phosphositeplus.1010730 21183079 Q6ZPJ3 426 S Phosphorylation Phosphositeplus.1010730 21183079 Q6ZPJ3 436 S Phosphorylation Phosphositeplus.1010730 20415495;21183079 Q6ZPJ3 487 S Phosphorylation Phosphositeplus.1010730 21183079 Q6ZPJ3 510 S Phosphorylation Phosphositeplus.1010730 21183079;21659604 Q6ZPJ3 82 S Phosphorylation Phosphositeplus.1010730 17242355;19367708;19737024;19854140;20415495;21183079;21659605 Q6ZPJ3 833 S Phosphorylation Phosphositeplus.1010730 17242355;19737024;19854140;20415495;21183079;21659604;21659605 Q6ZPJ3 836 S Phosphorylation Phosphositeplus.1010730 17242355;19737024;20415495;21183079;21659604;21659605 Q6ZPJ3 84 S Phosphorylation Phosphositeplus.1010730 17242355;19367708;19737024;19854140;20415495;21183079;21659605 Q6ZPJ3 893 S Phosphorylation Phosphositeplus.1010730 18846507;19367708;19854140;20415495;21183079 Q6ZPJ3 93 S Phosphorylation Phosphositeplus.1010730 19854140;21183079;21659605 Q6ZPJ3 94 S Phosphorylation Phosphositeplus.1010730 19854140;21183079;21659605 Q6ZPJ3 97 S Phosphorylation Phosphositeplus.1010730 19854140;21183079 Q6ZPJ3 1110 T Phosphorylation Phosphositeplus.1010730 20531401 Q6ZPJ3 483 T Phosphorylation Phosphositeplus.1010730 21183079 Q6ZPJ3 835 T Phosphorylation Phosphositeplus.1010730 20531401;21183079;21659604;21659605 Q6ZPJ3 638 Y Phosphorylation Phosphositeplus.1010730 18563927 Q6ZPJ3 824 M Oxidation SysPTM 1.1 17242355 Q6ZPJ3 82 S Phosphorylation SysPTM 1.1 17242355 Q6ZPJ3 84 S Phosphorylation SysPTM 1.1 17242355 Q6ZPJ3 833 S Phosphorylation SysPTM 1.1 17242355 Q6ZPJ3 836 S Phosphorylation SysPTM 1.1 17242355 Q6ZPJ3 893 S Phosphorylation SysPTM 1.1 18846507 Q6ZPJ3 638 Y Phosphorylation SysPTM 1.1 18563927

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q6ZPJ3 836 S 17242355 HTP Phosphorylation Q6ZPJ3 82 S 17242355 HTP Phosphorylation Q6ZPJ3 833 S 17242355 HTP Phosphorylation Q6ZPJ3 84 S 17242355 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q6ZPJ3 82 Phosphoserine 17242355;21183079 Q6ZPJ3 84 Phosphoserine 17242355;21183079 Q6ZPJ3 394 Phosphoserine 21183079 Q6ZPJ3 436 Phosphoserine 21183079 Q6ZPJ3 833 Phosphoserine 17242355;21183079 Q6ZPJ3 835 Phosphothreonine 21183079 Q6ZPJ3 836 Phosphoserine 17242355;21183079

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type Ube2o IPI00453803 S 1196 GEASQGpSDSEGGA phosphorylation Ube2o IPI00453803 S 436 QPDGSApSPVEMQD phosphorylation Ube2o IPI00453803 S 82 LIHGEDpSDSEGDD phosphorylation Ube2o IPI00453803 S 833 EQLLTGpSPTSPTV phosphorylation Ube2o IPI00453803 S 836 LTGSPTpSPTVEPE phosphorylation Ube2o IPI00453803 S 84 HGEDSDpSEGDDDG phosphorylation Ube2o IPI00453803 S 893 EKPEVQpSPVKAEW phosphorylation Ube2o IPI00453803 S 93 DDDGRGpSSGCSEA phosphorylation Ube2o IPI00453803 S 94 DDGRGSpSGCSEAG phosphorylation Ube2o IPI00453803 S 97 RGSSGCpSEAGGAG phosphorylation Ube2o IPI00453803 T 835 LLTGSPpTSPTVEP phosphorylation Ube2o IPI00453803 T 838 GSPTSPpTVEPEKP phosphorylation

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID Ube2o Mus musculus (Mouse) 816; 822; 667; 843; 849; 940; 863; 987; 812; 516; 588; 127; 212 "816,ELKEAIKILESLK; 822,KILESLKNMTVEQ; 667,EDGALPKEDEPSV; 843,PTVEPEKPTREKK; 849,KPTREKKFLDDIK; 940,SNHSFKKIEFQPP; 863,LQENLKKTLDNVA; 987,LFSALIKGPTRTP; 812,KSFRELKEAIKIL; 516,SIPLSIKNLKRKH; 588,GDFVVDKRVQSCP; 127,WYPEGVKQHVKET; 212,GKVYDLKNQIILK" 816:22790023; 822:22790023; 667:22790023; 843:22790023; 849:22790023; 940:22790023; 863:22790023; 987:22790023; 812:22790023; 516:22790023; 588:22790023; 127:22790023; 212:22790023