PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus Tekt4 Sharpin 21829440 biogrid exp Eya2 Sharpin 20956555 biogrid,intact exp G G G G G Kmt2d Sharpin 21829440 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Psmd1 Sharpin 21829440 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Znf106 Sharpin 21829440 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Capn13 Sharpin 21829440 biogrid exp Sharpin Scn11a 21829440 biogrid exp G G G Surf6 Sharpin 21829440 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G Cux1 Sharpin 21829440 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Eya1 Sharpin 20956555 intact exp Traf2 Sharpin 21829440,15102471 biogrid,intact exp G G G G G G G G G G G G G G G G G G G G G G G G G Sharpin Uchl1 21829440 biogrid exp G G G G G G G G G G G G G G G G G G Rnf20 Sharpin 21829440 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Cebpa Sharpin 21829440 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G Sharpin Aifm1 21829440 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Sharpin Stk24 21829440 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Adcyap1 Sharpin 21829440 biogrid exp G G G G G G Asap2 Sharpin 21829440 biogrid exp Fyb Sharpin 21829440 biogrid exp G G G G G G G G G G G G G G G G G G Sharpin Eya4 20956555 biogrid exp G Sharpin Rnf31 21455180,24461064,21455181,23942237 biogrid,intact,dip exp, ortho Itga5 Sharpin 21947080 biogrid exp, ortho G G G G G G G G G G Sharpin Rbck1 21455180,21455181,23942237 biogrid,intact,dip exp, ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ikbkg Sharpin 21455181 dip exp, ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Itga2 Sharpin ortho G G G Pten Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Sharpin Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Otulin Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Tnfaip3 Sharpin ortho G G G G G Ikbkb Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Dlg4 Sharpin ortho G G G G G G G G G G Bdkrb2 Sharpin ortho Shank1 Sharpin ortho Tnfrsf1a Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Itgam Sharpin ortho G G G G G G G G G Tnf Sharpin ortho Ltbr Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Itgad Sharpin ortho Chuk Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Shank2 Sharpin ortho Il1a Sharpin ortho G Nod2 Sharpin ortho Shank3 Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G Tfap2c Sharpin ortho G G G G G Aurkb Sharpin ortho G G G G G G G G G G G G G Sharpin Grip1 ortho G G G G G G G G G G G Itgb1 Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Txn2 Sharpin ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Sharpin Lipf ortho Itgal Sharpin ortho G G G G Itga1 Sharpin ortho

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q5DTM8 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q91WA6 Q9R053 affinity chromatography technology 21829440 physical association X 2 Q91WA6 P53564-2 affinity chromatography technology 21829440 physical association X 2 Q91WA6 Q924T7 affinity chromatography technology 24461064 physical association X 2 Q91WA6 P97767-2 two hybrid 20956555 direct interaction X 2 Q91WA6 P97767-2 affinity chromatography technology 20956555 physical association X 2 Q91WA6 P97767-2 affinity chromatography technology 20956555 physical association X 2 Q91WA6 Q924T7 affinity chromatography technology 21455181 physical association X 2 Q91WA6 Q924T7 affinity chromatography technology 21455181 physical association X 2 O35601 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q9R0P9 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q99KH8 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q91WA6 affinity chromatography technology 21829440 physical association X 2 P39429 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q3UW68 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q91WA6 P53566 affinity chromatography technology 21829440 physical association X 2 Q91WA6 Q3TXS7 affinity chromatography technology 21829440 physical association X 2 Q91WA6 Q9Z0X1 affinity chromatography technology 21829440 physical association X 2 Q91WA6 P11688 fluorescent resonance energy transfer 21947080 direct interaction X 2 Q91WA6 O08575 two hybrid 20956555 direct interaction X 2 Q91WA6 O88522 enzymatic study 21455181 direct interaction X 2 Q9WUB0 Q91WA6 affinity chromatography technology 21455181 physical association X 2 Q9WUB0 Q91WA6 affinity chromatography technology 21455181 physical association X 2 P70279 Q91WA6 affinity chromatography technology 21829440 physical association X 2 O70176 Q91WA6 affinity chromatography technology 21829440 physical association X 2 Q91WA6 anti bait coimmunoprecipitation 21455181 - C 3 Q91WA6 Q924T7 molecular sieving 21455180 physical association X 2 Q91WA6 Q924T7 anti bait coimmunoprecipitation 21455181 physical association X 2 Q91WA6 Q924T7 anti bait coimmunoprecipitation 21455180 physical association X 2 Q91WA6 Q924T7 anti bait coimmunoprecipitation 21455181 physical association X 2 Q91WA6 Q924T7 anti bait coimmunoprecipitation 21455181 physical association X 2 Q91WA6 Q924T7 anti bait coimmunoprecipitation 21455180 physical association X 2 Q91WA6 Q924T7 anti bait coimmunoprecipitation 21455180 physical association X 2 Q91WA6 Q924T7 anti bait coimmunoprecipitation 21455180 physical association X 2 Q91WA6 Q924T7 anti bait coimmunoprecipitation 21455181 physical association X 2 Q91WA6 anti bait coimmunoprecipitation 21455180 physical association C 3 Q91WA6 molecular sieving 21455180 physical association C 3 Q91WA6 anti bait coimmunoprecipitation 21455180 physical association C 3 Q91WA6 anti bait coimmunoprecipitation 21455181 physical association C 3 Q91WA6 molecular sieving 21455180 physical association C 3 Q91WA6 anti bait coimmunoprecipitation 21455180 physical association C 3 Q91WA6 O88522 anti bait coimmunoprecipitation 21455181 physical association X 2 Q9WUB0 Q91WA6 anti bait coimmunoprecipitation 21455180 physical association X 2 Q9WUB0 Q91WA6 anti bait coimmunoprecipitation 21455180 physical association X 2 Q91WA6 anti bait coimmunoprecipitation 23942237 association C 4 Q91WA6 P97767 pull down 20956555 physical association X 2 Q91WA6 P97767 two hybrid 20956555 physical association X 2 Q91WA6 P97767 anti tag coimmunoprecipitation 20956555 physical association X 2 Q91WA6 P97767 two hybrid 20956555 physical association X 2 Q91WA6 molecular sieving 23942237 association C 3 P39429 Q91WA6 two hybrid 15102471 physical association X 2 P39429 Q91WA6 two hybrid 15102471 physical association X 2 Q91WA6 O08575 two hybrid 20956555 physical association X 2 Q91WA6 O08575 pull down 20956555 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B Rnf20 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321371 prey bait Sharpin Rbck1 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 21455181 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid) BIOGRID_316228|BIOGRID_316229 prey|bait bait|prey Sharpin Uchl1 MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321373 bait prey Traf2 Sharpin MI:0018(two hybrid)|MI:0004(affinity chromatography technology)|MI:0018(two hybrid) 15102471,21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid)|(intact) UniProt_163226|BIOGRID_321364|UniProt_163225 bait|prey|bait prey|bait|prey Sharpin Scn11a MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321360 bait prey Cebpa Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321368 prey bait Sharpin Stk24 MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321362 bait prey Adcyap1 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321370 prey bait Sharpin Aifm1 MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321372 bait prey Cux1 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321363 prey bait Psmd1 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321369 prey bait Nsd1 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321366 prey bait Eya2 Sharpin MI:0018(two hybrid)|MI:0096(pull down)|MI:0096(pull down) 20956555 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact)|(intact) BIOGRID_314695|UniProt_172358|UniProt_172360 bait|prey|bait prey|bait|prey Ikbkg Sharpin MI:0004(affinity chromatography technology) 21455181 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction) (biogrid) BIOGRID_316231 prey bait Asap2 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321367 prey bait Capn13 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321375 prey bait Tekt4 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321365 prey bait Surf6 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321374 prey bait Fyb Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321359 prey bait Itga5 Sharpin MI:0004(affinity chromatography technology) 21947080 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction) (biogrid) BIOGRID_322973 bait prey Rnf31 Sharpin MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 21455181,24461064 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid)|(biogrid) BIOGRID_332973|BIOGRID_316230|BIOGRID_316226 bait|prey|bait prey|bait|prey Eya1 Sharpin MI:0096(pull down)|MI:0096(pull down)|MI:0096(pull down)|MI:0096(pull down) 20956555 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association) (intact)|(intact)|(intact)|(intact) UniProt_172359|UniProt_172362|UniProt_172361|UniProt_172365 prey|bait|bait|bait bait|prey|prey|prey Zfp106 Sharpin MI:0004(affinity chromatography technology) 21829440 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_321361 prey bait

PPI - HINT Gene A Gene B PMID Method Quality EYA2 SHARPIN 20956555,20956555 0018,0096 LC IKBKG SHARPIN 21455181,21455181 0006,0415 LC ITGA5 SHARPIN 21947080 0055 LC TRAF2 SHARPIN 15102471 0018 LC EYA1 SHARPIN 20956555,20956555,20956555,20956555 0018,0096,0004,0007 LC SHARPIN RNF31 21455180,21455180 0071,0006 LC SHARPIN RBCK1 23942237,23942237 0006,0071 LC EYA2 SHARPIN 20956555,20956555 0018,0096 LC FYB SHARPIN 21829440 0004 LC ADCYAP1 SHARPIN 21829440 0004 LC ZNF106 SHARPIN 21829440 0004 LC NSD1 SHARPIN 21829440 0004 LC IKBKG SHARPIN 21455181,21455181 0006,0415 LC TRAF2 SHARPIN 21829440 0004 LC CUX1 SHARPIN 21829440 0004 LC CEBPA SHARPIN 21829440 0004 LC SURF6 SHARPIN 21829440 0004 LC EYA1 SHARPIN 20956555,20956555,20956555,20956555 0018,0096,0004,0007 LC TEKT4 SHARPIN 21829440 0004 LC PSMD1 SHARPIN 21829440 0004 LC CAPN13 SHARPIN 21829440 0004 LC RNF20 SHARPIN 21829440 0004 LC ASAP2 SHARPIN 21829440 0004 LC SHARPIN RNF31 21455180,24461064,23942237,21455181,21455180,21455181 0071,0004,0006,0004,0006,0006 LC SHARPIN STK24 21829440 0004 LC SHARPIN SCN11A 21829440 0004 LC SHARPIN UCHL1 21829440 0004 LC SHARPIN RBCK1 23942237,21455181,21455180,23942237 0006,0004,0006,0071 LC SHARPIN AIFM1 21829440 0004 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q91WA6 P11688 SHARPIN ITGA5 0.309 21947080 Q9WUB0 Q91WA6 RBCK1 SHARPIN 0.61 21455181,23942237 Q91WA6 P39429 SHARPIN TRAF2 0.354 15102471,21829440 Q924T7 Q91WA6 RNF31 SHARPIN 0.61 21455181,24461064,23942237 Q91WA6 O08575 SHARPIN EYA2 0.623 20956555 Q91WA6 Q5DTM8 SHARPIN RNF20 0.21 21829440 Q91WA6 P53564 SHARPIN CUX1 0.21 21829440 Q91WA6 O88466 SHARPIN ZNF106 0.21 21829440 Q91WA6 O35601 SHARPIN FYB 0.21 21829440 Q91WA6 O70176 SHARPIN ADCYAP1 0.21 21829440 Q91WA6 Q3UW68 SHARPIN CAPN13 0.21 21829440 Q91WA6 P70279 SHARPIN SURF6 0.21 21829440 Q91WA6 P53566 SHARPIN CEBPA 0.21 21829440 Q91WA6 Q3TXS7 SHARPIN PSMD1 0.21 21829440 Q91WA6 P97767 SHARPIN EYA1 0.814 20956555 Q91WA6 Q149S1 SHARPIN TEKT4 0.21 21829440 Q91WA6 Q7SIG6 SHARPIN ASAP2 0.21 21829440 Q99KH8 Q91WA6 STK24 SHARPIN 0.21 21829440 Q9Z0X1 Q91WA6 AIFM1 SHARPIN 0.21 21829440 Q9R053 Q91WA6 SCN11A SHARPIN 0.21 21829440 Q9R0P9 Q91WA6 UCHL1 SHARPIN 0.21 21829440