PTM - dbPAF Gene Name UniProt ID Position Type Species Tnk2; Ack1 O54967 102 S Mus musculus(Mouse) Tnk2; Ack1 O54967 104 T Mus musculus(Mouse) Tnk2; Ack1 O54967 107 S Mus musculus(Mouse) Tnk2; Ack1 O54967 113 T Mus musculus(Mouse) Tnk2; Ack1 O54967 284 Y Mus musculus(Mouse) Tnk2; Ack1 O54967 33 T Mus musculus(Mouse) Tnk2; Ack1 O54967 40 Y Mus musculus(Mouse) Tnk2; Ack1 O54967 528 T Mus musculus(Mouse) Tnk2; Ack1 O54967 532 T Mus musculus(Mouse) Tnk2; Ack1 O54967 533 Y Mus musculus(Mouse) Tnk2; Ack1 O54967 578 S Mus musculus(Mouse) Tnk2; Ack1 O54967 579 S Mus musculus(Mouse) Tnk2; Ack1 O54967 618 T Mus musculus(Mouse) Tnk2; Ack1 O54967 622 S Mus musculus(Mouse) Tnk2; Ack1 O54967 624 T Mus musculus(Mouse) Tnk2; Ack1 O54967 650 Y Mus musculus(Mouse) Tnk2; Ack1 O54967 737 T Mus musculus(Mouse) Tnk2; Ack1 O54967 741 T Mus musculus(Mouse) Tnk2; Ack1 O54967 743 S Mus musculus(Mouse) Tnk2; Ack1 O54967 772 S Mus musculus(Mouse) Tnk2; Ack1 O54967 776 S Mus musculus(Mouse) Tnk2; Ack1 O54967 789 S Mus musculus(Mouse) Tnk2; Ack1 O54967 800 S Mus musculus(Mouse) Tnk2; Ack1 O54967 804 S Mus musculus(Mouse) Tnk2; Ack1 O54967 806 T Mus musculus(Mouse) Tnk2; Ack1 O54967 808 S Mus musculus(Mouse) Tnk2; Ack1 O54967 815 S Mus musculus(Mouse) Tnk2; Ack1 O54967 842 Y Mus musculus(Mouse) Tnk2; Ack1 O54967 844 T Mus musculus(Mouse) Tnk2; Ack1 O54967 870 S Mus musculus(Mouse) Tnk2; Ack1 O54967 871 S Mus musculus(Mouse) Tnk2; Ack1 O54967 874 Y Mus musculus(Mouse) Tnk2; Ack1 O54967 875 Y Mus musculus(Mouse) Tnk2; Ack1 O54967 896 S Mus musculus(Mouse) Tnk2; Ack1 O54967 951 S Mus musculus(Mouse)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA O54967 R854-m1 Methylation VIQAPGPrAGPCILP O54967 Y40-p Phosphorylation TRLSHFEyVKNEDLE O54967 S102-p Phosphorylation QSTFRKPsPTPGSLP O54967 T113-p Phosphorylation GSLPGEGtLQSLTCL O54967 Y284-p Phosphorylation LPQNDDHyVMQEHRK O54967 T528-p Phosphorylation PPQPAIFtQKTtyDP O54967 T532-p Phosphorylation AIFtQKTtyDPVSED O54967 Y533-p Phosphorylation IFtQKTtyDPVSEDP O54967 T618-p Phosphorylation ACSLLDKtPPQsPTR O54967 S622-p Phosphorylation LDKtPPQsPTRALPR O54967 Y650-p Phosphorylation PLPPPPAyDDVAQDE O54967 T741-p Phosphorylation QVPTGQLtPsPTPGG O54967 S743-p Phosphorylation PTGQLtPsPTPGGDD O54967 S772-p Phosphorylation TRPRVELsPAPsGEE O54967 S776-p Phosphorylation VELsPAPsGEEETSR O54967 S789-p Phosphorylation SRWPGPAsPPRVPPR O54967 S800-p Phosphorylation VPPREPLsPQGSRTP O54967 Y842-p Phosphorylation SFASDPKyAtPQVIQ O54967 T844-p Phosphorylation ASDPKyAtPQVIQAP O54967 S870-p Phosphorylation VRDGRKVssTHyyLL O54967 S871-p Phosphorylation RDGRKVssTHyyLLP O54967 Y874-p Phosphorylation RKVssTHyyLLPERP O54967 Y875-p Phosphorylation KVssTHyyLLPERPP O54967 S896-p Phosphorylation RFLREAQsPEEPAAL O54967 S951-p Phosphorylation NPGARPPsLRAAARL O54967 K131-ub Ubiquitination KDLRLLEkLGDGSFG O54967 K565-ub Ubiquitination PRGLWLAkPSARVPG

PTM - dbPTM UniProt ID Position Code Type Resources References O54967 533 Y Phosphorylation Swiss-Prot 1010711 18034455;21169560 O54967 874 Y Phosphorylation Swiss-Prot 1010711 18034455 O54967 842 Y Phosphorylation PhosphoELM.10011 14739304 O54967 622 S Phosphorylation Phosphositeplus.1010730 20415495 O54967 772 S Phosphorylation Phosphositeplus.1010730 16512673;18268350;19367708;19854140;21659604 O54967 789 S Phosphorylation Phosphositeplus.1010730 20415495;21183079;21659604;21659605 O54967 871 S Phosphorylation Phosphositeplus.1010730 18563927 O54967 896 S Phosphorylation Phosphositeplus.1010730 20415495 O54967 113 T Phosphorylation Phosphositeplus.1010730 20415495;21183079;21659605 O54967 33 T Phosphorylation Phosphositeplus.1010730 18056256 O54967 618 T Phosphorylation Phosphositeplus.1010730 20415495 O54967 284 Y Phosphorylation Phosphositeplus.1010730 16873679;18515860;18563927;20116462;20333297;20623637 O54967 650 Y Phosphorylation Phosphositeplus.1010730 18563927 O54967 842 Y Phosphorylation Phosphositeplus.1010730 14739304;18268350;18515860;18563927;20116462 O54967 874 Y Phosphorylation Phosphositeplus.1010730 16873679;18515860;18563927;20116462 O54967 875 Y Phosphorylation Phosphositeplus.1010730 18515860;18563927 O54967 33 T Phosphorylation SysPTM 1.1 18056256 O54967 284 Y Phosphorylation SysPTM 1.1 18515860;18563927 O54967 650 Y Phosphorylation SysPTM 1.1 18563927 O54967 842 Y Phosphorylation SysPTM 1.1 18515860;18563927 O54967 874 Y Phosphorylation SysPTM 1.1 18515860;18034455;18563927 O54967 875 Y Phosphorylation SysPTM 1.1 18515860;18563927

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type O54967 842 Y 14739304 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs O54967 113 Phosphothreonine 21183079 O54967 533 Phosphotyrosine 21169560 O54967 854 Omega-N-methylarginine 24129315

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type Tnk2 IPI00119965 S 622 DKTPPQpSPTRALP phosphorylation Tnk2 IPI00119965 S 789 RWPGPApSPPRVPP phosphorylation Tnk2 IPI00119965 S 896 FLREAQpSPEEPAA phosphorylation Tnk2 IPI00119965 T 113 SLPGEGpTLQSLTC phosphorylation Tnk2 IPI00119965 T 618 CSLLDKpTPPQSPT phosphorylation

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID Tnk2 Mus musculus (Mouse) 131; 565 "131,DLRLLEKLGDGSF; 565,RGLWLAKPSARVP" 131:22790023; 565:22790023