PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus Fzd4 Usp8 20495530 mint,intact exp G G G G G G G G G G G G G G G G G G G G G G G G G G G Dnajb3 Usp8 15342353 biogrid exp G G Usp8 Hdac6 16810319 mint,intact exp GP G G G G G G G G G G G G G G G G G G G G G G G G G G G Psmd4 Usp8 16810319 mint,intact exp GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Rnf41 19527720 biogrid,mint,intact exp, ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Rasgrf1 Usp8 11500497 biogrid exp, ortho G G G G G G G G G G G G G G G G Ubc Usp8 16810319,24533902 biogrid exp, ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ywhae Usp8 17720156,16944949 biogrid,mint,intact exp, ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Grap2 Usp8 12176364 i2d,mint,intact exp, ortho Stam2 Usp8 20130268,10982817 biogrid,i2d,mint,intact exp, ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Grb2 Usp8 10982817 i2d,mint,intact exp, ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Stam Usp8 i2d exp, ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Tardbp ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Akt1 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Mcph1 Usp8 ortho Ube2e1 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp22 Usp8 ortho G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Chmp1b1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Scnn1b ortho G G G G G G G G G Usp8 Chmp2a ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Dnajb6 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Eps15 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ube2g1 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Ywhab ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ywhaz Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Acaa2 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Trim54 ortho G G G G G G G Pja1 Usp8 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Krt36 Usp8 ortho P Usp8 Rnf128 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Kif23 Usp8 ortho G G G G G G G G G G G G G G MCG19772, Protein Trim55 Usp8 ortho Slbp Usp8 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ptpn23 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G Usp8 Chmp1a ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ntrk1 Usp8 ortho Pten Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Vps4b Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Ywhag Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Scnn1a Usp8 ortho G G G G G G G G G G G G G G G G G G Nbr1 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Scnn1g ortho G G Ywhah Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Birc6 Usp8 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Vamp8 Usp8 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Chmp2b ortho P Chmp6 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Hif1a Usp8 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Kcnn4 Usp8 ortho G G G G G G G G G G G G G G G G Usp28 Usp8 ortho Erbb4 Usp8 ortho P Usp8 Pold3 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Chmp5 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp4 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Chmp7 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Sfn Usp8 ortho P G G G G G G G G Usp8 Usp15 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Chmp4c ortho P G G G G G G Usp8 Chmp4b ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Chmp3 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Stat6 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G Usp8 Usp21 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Egfr Usp8 ortho GP G G G G G G G G G G G G G G G G G Htt Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Epas1 Usp8 ortho G G G G G G G G G G G G G G G G G G G G G Src Usp8 ortho G G G G G G G G G G G G G G G G G G G G G G G G G Tsen2 Usp8 ortho Usp8 Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Tatdn1 Usp8 ortho Ube2i Usp8 ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Trim63 Usp8 ortho Usp8 Farsb ortho GP G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Spp1 Usp8 ortho G G G G G G G G G G G G G G G G G G G G G G G G Lrig1 Usp8 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants P70297 Q80U87 - 10982817 - X 2 Q60631 Q80U87 - 10982817 - X 2 Q80U87 O89100 - 12176364 - X 2 Q80U87 - 11500497 - X 2 Q80U87 O88811 - 10982817 - X 2 Q80U87 anti tag coimmunoprecipitation 18329369 association C 3 Q80U87 P62259 coimmunoprecipitation 17720156 association X 2 Q60631 Q80U87 pull down 10982817 direct interaction X 2 Q80U87 O88811 coimmunoprecipitation 10982817 physical association X 2 Q80U87 O88811 filter binding 10982817 direct interaction X 2 Q80U87 O89100 lambda phage display 12176364 physical association X 2 Q80U87 O89100 lambda phage display 12176364 physical association X 2 Q80U87 O89100 filter binding 12176364 direct interaction X 2 Q80U87 O89100 filter binding 12176364 direct interaction X 2 Q80U87 Q8CGC3 pull down 16810319 direct interaction X 2 Q80U87 O35226 pull down 16810319 direct interaction X 2 Q80U87 Q8BH75 anti bait coimmunoprecipitation 19527720 association X 2 Q61088 Q80U87 anti tag coimmunoprecipitation 20495530 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B Stam2 Usp8 MI:0019(coimmunoprecipitation)|MI:0096(pull down)|MI:0096(pull down)|MI:0019(coimmunoprecipitation)|MI:0019(coimmunoprecipitation)|MI:0004(affinity chromatography technology) 10982817,20130268 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association) (intact)|MI:0471(mint)|MI:0471(mint)|(intact)|(intact)|(biogrid) UniProt_161829|MINT_85470|MINT_85471|UniProt_69463|UniProt_161830|BIOGRID_319328 prey|prey|prey|prey|prey|prey bait|bait|bait|bait|bait|bait Psmd4 Usp8 MI:0071(molecular sieving)|MI:0071(molecular sieving) 16810319 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction)|MI:0407(direct interaction) (intact)|MI:0471(mint) UniProt_167739|MINT_82170 prey|prey bait|bait Usp8 Hdac6 MI:0071(molecular sieving)|MI:0071(molecular sieving) 16810319 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction)|MI:0407(direct interaction) (intact)|MI:0471(mint) UniProt_167738|MINT_82167 bait|bait prey|prey Usp8 Rnf41 MI:0006(anti bait coimmunoprecipitation)|MI:0006(anti bait coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19527720 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0914(association)|MI:0914(association)|MI:0915(physical association) (intact)|MI:0471(mint)|(biogrid) UniProt_171004|MINT_71623|BIOGRID_315986 prey|prey|prey bait|bait|bait Fzd4 Usp8 MI:0416(fluorescence microscopy)|MI:0416(fluorescence microscopy)|MI:0416(fluorescence microscopy) 10,20495530 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association) MI:0471(mint)|(intact)|(intact) MINT_78570|UniProt_107459|UniProt_170986 bait|bait|bait prey|prey|prey Ubc Usp8 MI:0096(pull down) 16810319 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction) (biogrid) BIOGRID_324154 prey bait Dnajb3 Usp8 MI:0047(far western blotting)|MI:0047(far western blotting) 15342353 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction)|MI:0407(direct interaction) (biogrid)|(biogrid) BIOGRID_310965|BIOGRID_310964 bait|bait prey|prey Rasgrf1 Usp8 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 11500497 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0407(direct interaction) (biogrid)|(biogrid) BIOGRID_320079|BIOGRID_320078 prey|prey bait|bait Grap2 Usp8 MI:0096(pull down)|MI:0049(filter binding)|MI:0096(pull down)|MI:0096(pull down)|MI:0049(filter binding)|MI:0049(filter binding)|MI:0049(filter binding)|MI:0096(pull down)|MI:0049(filter binding)|MI:0049(filter binding) 12176364 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0407(direct interaction) MI:0471(mint)|(intact)|MI:0471(mint)|MI:0471(mint)|(intact)|(intact)|(intact)|MI:0471(mint)|(intact)|(intact) MINT_61722|UniProt_71085|MINT_61721|MINT_61711|UniProt_163552|UniProt_71083|UniProt_163551|MINT_60105|UniProt_163559|UniProt_163557 bait|bait|bait|prey|prey|bait|prey|prey|bait|bait prey|prey|prey|bait|bait|prey|bait|bait|prey|prey Ywhae Usp8 MI:0004(affinity chromatography technology)|MI:0019(coimmunoprecipitation)|MI:0019(coimmunoprecipitation)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 16944949,17720156 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0914(association)|MI:0914(association)|MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact)|MI:0471(mint)|(biogrid)|(biogrid) BIOGRID_319107|UniProt_169759|MINT_79165|BIOGRID_316583|BIOGRID_316061 bait|unspecified role|unspecified role|bait|bait prey|unspecified role|unspecified role|prey|prey Grb2 Usp8 MI:0019(coimmunoprecipitation)|MI:0096(pull down)|MI:0019(coimmunoprecipitation) 10982817 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction) (intact)|MI:0471(mint)|(intact) UniProt_69465|MINT_78194|UniProt_161832 prey|prey|prey bait|bait|bait

PPI - HINT Gene A Gene B PMID Method Quality STAM2 USP8 10982817,10982817 0019,0049 LC GRAP2 USP8 12176364,12176364,12176364,12176364,12176364 0047,0045,0004,0049,0066 LC UBC USP8 16810319 0415 LC YWHAE USP8 17720156 0019 LC DNAJB3 USP8 15342353,15342353 0047,0096 LC PSMD4 USP8 16810319 0096 LC STAM2 USP8 20130268 0004 LC GRAP2 USP8 12176364,12176364,12176364,12176364,12176364 0047,0045,0004,0049,0066 LC UBC USP8 24533902 0096 LC RASGRF1 USP8 11500497,11500497 0004,0096 LC YWHAE USP8 16944949,16944949 0096,0004 LC DNAJB3 USP8 15342353,15342353 0047,0096 LC GRB2 USP8 10982817 0096 LC FZD4 USP8 20495530 0007 LC USP8 RNF41 19527720,19527720 0004,0006 LC USP8 HDAC6 16810319 0096 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q80U87 Q61088 USP8 FZD4 0.21 20495530 Q8CGC3 Q80U87 HDAC6 USP8 0.309 16810319 Q80U87 O35226 USP8 PSMD4 0.309 16810319 Q80U87 O88811 USP8 STAM2 0.569 10982817,20130268 Q80U87 P61981 USP8 YWHAG 0.21 17720156 Q80U87 P62258 USP8 YWHAE 0.21 17720156 Q80U87 P62259 USP8 YWHAE 0.436 17720156,16944949 Q80U87 P63104 USP8 YWHAZ 0.21 17720156 Q80U87 Q02241 USP8 KIF23 0.21 18329369 Q80U87 Q60631 USP8 GRB2 0.309 10982817 Q9NR09 Q80U87 BIRC6 USP8 0.21 18329369 Q80U87 O89100 USP8 GRAP2 0.623 12176364 Q80U87 P00533 USP8 EGFR 0.702 16120644,17720156,17121848 Q80U87 P27671 USP8 RASGRF1 0.454 11500497 Q80U87 P0CG50 USP8 UBC 0.523 16810319,24533902 Q8BH75 Q80U87 RNF41 USP8 0.376 19527720 Q80U87 O35723 USP8 DNAJB3 0.523 15342353