PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus Nsfl1c Rad23b 19182904 biogrid exp G G G G G G G G Nsfl1c Vcp 16601695,20691684,12847084,9214505,10811609 biogrid,mint,intact exp, ortho G G G G G G G G Nsfl1c Nploc4 10811609 biogrid exp, ortho G G G G G G G G Nsfl1c Nsfl1c 16601695 mint,intact exp, ortho G G G G G G G G Nsfl1c Yod1 ortho Protein Tsr1, RCG34104, isoform CRA_a Nsfl1c ortho G G G G G G G G Nsfl1c Uba3 ortho G G G G G G G G Nsfl1c Nae1 ortho G G G G G G G G Nsfl1c Hk1 ortho G G G G G G G G Nsfl1c Cdk2 ortho FKBP-associated protein, isoform CRA_a, Protein Glmn Nsfl1c ortho G G G G G G G G Nsfl1c Fbp1 ortho G G Nsfl1c Arhgef7 ortho G G G G G G G G Nsfl1c Ppp1ca ortho G G G G G G G G Nsfl1c Cct4 ortho G G G G G G G G Nsfl1c GDP-mannose 4, 6-dehydratase, Protein Gmds, RCG44147 ortho Fam32a Nsfl1c ortho G G G G G G G G RGD1309519_predicted Nsfl1c ortho Nsfl1c Med4 ortho G G G G G G G G Nsfl1c Phax ortho G G G G G G G G Nsfl1c Capn2 ortho G G G G G G G G Nsfl1c Nuf2 ortho Nsfl1c Ywhae ortho G G G G G G G G Nsfl1c Ufd1l ortho G G G G G G G G Nsfl1c Plaa ortho G G G G G G G G Sprtn Nsfl1c ortho Ldb2_predicted Nsfl1c ortho Ribosomal protein S6 kinase Nsfl1c ortho G G G G G G G G Nsfl1c Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 14, Protein Psmd14, Psmd14 protein, RCG26455, isoform CRA_b ortho Nsfl1c Cnbp ortho G G G G G G G G Ubr1 Nsfl1c ortho G G G G G G G G Pfdn5_predicted Nsfl1c ortho G G G G G G G G Nsfl1c Gsk3b ortho G G Nsfl1c Stx5 ortho G G G G G G G G Protein Srp14, RCG26543, isoform CRA_b, Signal recognition particle 14 Nsfl1c ortho G G G G G G G G Nsfl1c Cdv3 ortho Hif1a Nsfl1c ortho G G G G G G G G Nsfl1c Crk ortho G G G G G G G G Nsfl1c Prmt1 ortho Nsfl1c Eif2b2 ortho G G G G G G G Cul2_predicted Nsfl1c ortho G G G G G Nsfl1c Prkacb ortho Nsfl1c Psmc1 ortho G G G G G G G G Nsfl1c Ubc ortho Protein Sfn, RCG31390 Nsfl1c ortho Dnaja2 Nsfl1c ortho G G G G G G G G Nsfl1c Ubxn4 ortho G G G G G G G G Nsfl1c Fbxo7 ortho G G G G G G G G Cul1_predicted Nsfl1c ortho G G G G G G G G Nsfl1c Bax ortho G G G G G G G Nsfl1c Uba5 ortho G G G G G G G G Protein Parp6 Nsfl1c ortho G G G G G G Nsfl1c Fh ortho G G G G G G G G Nsfl1c Cdk1 ortho G G G Nsfl1c Vcpip1 ortho Nsfl1c Cct2 ortho G G G G G G G G Nsfl1c Rpl22 ortho G G G G G G G G Nsfl1c Dbi ortho G G G G G G G G Nsfl1c Intestinal-type alkaline phosphatase 2, IAP-2, Intestinal alkaline phosphatase 2 ortho Sh3bgrl_predicted Nsfl1c ortho Cct8_predicted Nsfl1c ortho G G G G G G G G Nsfl1c Nf1 ortho Atic Nsfl1c ortho Itpa Nsfl1c ortho G G G G G G G G Nsfl1c Gabarapl1 ortho G G G G G G G G Nsfl1c Btrc ortho G G G G G G G G Nsfl1c Lin54 ortho Nsfl1c Ester hydrolase C11orf54 homolog ortho G G G G G G G G Nsfl1c Ikbkg ortho Nsfl1c Hsph1 ortho G G G G G G G G Dnajb1_predicted Nsfl1c ortho G G G G G G G G Msn Nsfl1c ortho G G G G G G G G Nsfl1c Abi1 ortho G G G G G G G G Protein Shfm1, RCG28037 Nsfl1c ortho G G G G G G G G Nsfl1c Tufm ortho G G G G G G G G Nsfl1c Aldoc ortho G G G G Nsfl1c Cdk5 ortho G G G G G G G Nsfl1c Cct3 ortho G G G G G G G G Protein Pdxdc1, Similar to expressed sequence AA415817 (Predicted) Nsfl1c ortho Nsfl1c Dlg4 ortho G G G G G G Nsfl1c Hspa4 ortho G G G G G G G G Nsfl1c Ngly1 ortho Cul3 Nsfl1c ortho G G G G G G G G Il7r_predicted Nsfl1c ortho Nsfl1c Tubb2a ortho G G G G G G G G Nsfl1c Ahcy ortho G G G G G G G G Nsfl1c Lipe ortho G Nsfl1c Psmd2 ortho G G G G G G G G Prmt5_predicted Nsfl1c ortho G G G G G G G G Nsfl1c Pdk1 ortho G G G G G Slk Nsfl1c ortho DEAH (Asp-Glu-Ala-His) box polypeptide 15 (Predicted), isoform CRA_b, Protein Dhx15 Nsfl1c ortho Nsfl1c Ppp1r2 ortho G G G G G G G G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants O35987 - 10811609,9214505 - C 9 O35987 - 10811609,9214505 - C 9 O35987 - 10811609,9214505 - C 9 O35987 - 9214505 - C 3 O35987 - 9214505 - C 3 O35987 - 9214505 - C 3 P46462 O35987 affinity chromatography technology 12847084 physical association X 2 P46462 O35987 affinity chromatography technology 10811609 physical association X 2 P46462 O35987 biochemical 10811609 colocalization X 2 P46462 O35987 affinity chromatography technology 10811609 physical association X 2 Q9ES54 O35987 affinity chromatography technology 10811609 physical association X 2 Q4KMA2 O35987 affinity chromatography technology 19182904 physical association X 2 O35987 electron microscopy 10930451 association C 3 P46462 O35987 coimmunoprecipitation 11740563 association X 2 P46462 O35987 coimmunoprecipitation 10811609 association X 2 P46462 O35987 molecular sieving 9214505 association X 2 O35987 anti tag coimmunoprecipitation 18775313 association C 4 O35987 anti tag coimmunoprecipitation 18775313 association C 4 O35987 anti tag coimmunoprecipitation 18775313 association C 5 P46462 O35987 isothermal titration calorimetry 16601695 direct interaction X 2 P46462 O35987 isothermal titration calorimetry 16601695 direct interaction X 2 P46462 O35987 electron microscopy 16601695 direct interaction X 2 P46462 O35987 molecular sieving 16601695 colocalization X 2 P46462 O35987 competition binding 20691684 direct interaction X 2 P46462 O35987 pull down 20691684 direct interaction X 2 P46462 O35987 molecular sieving 9214505 physical association X 2 P46462 O35987 anti tag coimmunoprecipitation 9214505 physical association X 2 P46462 O35987 anti bait coimmunoprecipitation 9214505 physical association X 2 P46462 O35987 molecular sieving 9214505 physical association X 2 Q01853 O35987 pull down 20691684 direct interaction X 2 Q01853 O35987 pull down 20691684 direct interaction X 2 Q01853 O35987 pull down 20691684 direct interaction X 2 O35987 anti tag coimmunoprecipitation 18775313 association C 5 O35987 anti tag coimmunoprecipitation 18775313 association C 6 O35987 pull down 18656546 physical association C 3 O35987 anti tag coimmunoprecipitation 18775313 association C 3 O35987 O35987 biochemical 16601695 direct interaction X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B Nsfl1c Rad23b MI:0004(affinity chromatography technology) 19182904 taxid:10116(Rattus norvegicus) taxid:10116(Rattus norvegicus) MI:0915(physical association) (biogrid) BIOGRID_334900 prey bait Nsfl1c Nploc4 MI:0004(affinity chromatography technology) 10811609 taxid:10116(Rattus norvegicus) taxid:10116(Rattus norvegicus) MI:0915(physical association) (biogrid) BIOGRID_336152 bait prey Nsfl1c Vcp MI:0096(pull down)|MI:0096(pull down)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0415(enzymatic study)|MI:0096(pull down)|MI:0071(molecular sieving)|MI:0401(biochemical)|MI:0071(molecular sieving)|MI:0071(molecular sieving)|MI:0071(molecular sieving)|MI:0096(pull down)|MI:0401(biochemical)|MI:0401(biochemical)|MI:0096(pull down)|MI:0401(biochemical)|MI:0401(biochemical)|MI:0401(biochemical)|MI:0401(biochemical)|MI:0401(biochemical) 10811609,12847084,16601695,20691684,9214505 taxid:10116(Rattus norvegicus) taxid:10116(Rattus norvegicus) MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0403(colocalization)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0403(colocalization)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0403(colocalization)|MI:0407(direct interaction) MI:0471(mint)|(intact)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(intact)|(intact)|MI:0471(mint)|(intact)|(intact)|(intact)|(intact)|(intact)|(intact)|MI:0471(mint)|(intact)|MI:0471(mint)|(intact)|MI:0471(mint)|MI:0471(mint) MINT_78644|UniProt_173356|BIOGRID_336151|BIOGRID_336139|BIOGRID_336153|BIOGRID_335266|UniProt_185733|UniProt_186450|MINT_80888|UniProt_186451|UniProt_186452|UniProt_186453|UniProt_185737|UniProt_183335|UniProt_183334|MINT_78643|UniProt_183336|MINT_80890|UniProt_183333|MINT_80894|MINT_80892 neutral component|prey|prey|prey|bait|prey|prey|neutral component|neutral component|bait|bait|neutral component|prey|neutral component|neutral component|prey|neutral component|neutral component|neutral component|neutral component|neutral component bait|bait|bait|bait|prey|bait|bait|neutral component|neutral component|prey|prey|neutral component|bait|neutral component|neutral component|bait|neutral component|neutral component|neutral component|neutral component|neutral component Nsfl1c Nsfl1c MI:0401(biochemical)|MI:0401(biochemical) 16601695 taxid:10116(Rattus norvegicus) taxid:10116(Rattus norvegicus) MI:0407(direct interaction)|MI:0407(direct interaction) (intact)|MI:0471(mint) UniProt_183332|MINT_80887 neutral component|neutral component neutral component|neutral component

PPI - HINT Gene A Gene B PMID Method Quality NSFL1C NSFL1C 16601695 0401 LC NSFL1C VCP 10811609,9214505,10811609,10811609,9214505,10811609,16601695,9214505,10811609,16601695,20691684,20691684,11740563,16601695 0006,0006,0004,0401,0071,0071,0071,0007,0019,0065,0096,0405,0019,0040 LC NSFL1C NPLOC4 10811609,10811609,10811609 0071,0405,0004 LC NSFL1C NSFL1C 16601695 0401 LC NSFL1C VCP 10811609,9214505,10811609,10811609,9214505,10811609,9214505,10811609,12847084,20691684,20691684 0006,0006,0004,0401,0071,0071,0007,0019,0004,0096,0405 LC NSFL1C RAD23B 19182904 0004 LC NSFL1C NPLOC4 10811609,10811609,10811609 0071,0405,0004 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q96JH7 O35987 VCPIP1 NSFL1C 0.126 18775313 Q8TAT6 O35987 NPLOC4 NSFL1C 0.126 18775313 Q9UKV5 O35987 AMFR NSFL1C 0.126 18775313 Q9ES54 O35987 NPLOC4 NSFL1C 0.21 10811609 Q9Y263 O35987 PLAA NSFL1C 0.126 18775313 Q5T4S7 O35987 UBR4 NSFL1C 0.126 18775313 Q16531 O35987 DDB1 NSFL1C 0.126 18775313 P0CG50 O35987 UBC NSFL1C 0.309 12411482 Q13618 O35987 CUL3 NSFL1C 0.126 18775313 Q13617 O35987 CUL2 NSFL1C 0.126 18775313 Q13616 O35987 CUL1 NSFL1C 0.126 18775313 Q08380 O35987 LGALS3BP NSFL1C 0.126 18775313 O35987 O35987 NSFL1C NSFL1C 0.332 16601695 P55072 O35987 VCP NSFL1C 0.126 18775313 O95071 O35987 UBR5 NSFL1C 0.126 18775313 P46462 O35987 VCP NSFL1C 0.914 10811609,20691684,12847084,16601695 O95155 O35987 UBE4B NSFL1C 0.126 18775313 Q01853 O35987 VCP NSFL1C 0.309 20691684 Q4KMA2 O35987 RAD23B NSFL1C 0.21 19182904