iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-045959

UUCD1 version

UUC-HoS-01092

Ensembl Protein ID

ENSP00000229268.8

UniProt Accession

P45974; D3DUS7; D3DUS8; Q96J22; UBP5_HUMAN

Genbank Protein ID

CAA62690.1; AAC50465.1; AAB51314.1; AAB51315.1; AAA78934.1; EAW88724.1; EAW88725.1; EAW88726.1; EAW88727.1; AAH04889.1; AAH05139.1

Protein Name

Ubiquitin carboxyl-terminal hydrolase 5; Deubiquitinating enzyme 5; Isopeptidase T; Ubiquitin thioesterase 5; Ubiquitin-specific-processing protease 5

Genbank Nucleotide ID

X91349; U47927; U47924; U47924; U35116; CH471116; CH471116; CH471116; CH471116; BC004889; BC005139

Gene Name

USP5; ISOT

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000111667.13	ENST00000229268.12	ENSP00000229268.8
ENSG00000111667.13	ENST00000389231.9	ENSP00000373883.5
ENSG00000111667.13	ENST00000542087.1	ENSP00000444668.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	circBase	CircNet	miRTarBase
microRNA	TRANSFAC	TargetScan	RepTar
miRNAMap	SomamiR	miRcode	RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Protein 3D Structure
PDB	MMDB
Disease-associated Information
GWASdb	OMIM
Drug and target
TTD	GRAC
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	Phospho.ELM	UniProt	PHOSIDA
BioGRID	mUbiSiDa
DNA Methylation
TCGA	ICGC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
UBD/ZnF/ZnF_UBP	ZnF_UBP	16564012; 22283393
UBD/Alpha-Helix/UBA	UBA	16564012; 22283393
DUB/USP	USP	19489724; 21245847

Classification

Family	E-value	Score	Start	End
DUB/USP	2.80e-63	215.8	326	855
UBD/Alpha-Helix/UBA	3.30e-22	79.5	725	762

Active Site

Position(s)	Description	Evidence
335	Nucleophile
818	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrelllekkkeklneenpkepkgellkslqklfeel....................wkskkkavspre 72
           tG++NlgN+CY+NSv+Q+L++ip++++ + + k ek+ +++p++p+++++++++kl + l                    +k+ +  ++pr+
   Q: 326  TGIRNLGNSCYLNSVVQVLFSIPDFQRKYVD-KLEKIFQNAPTDPTQDFSTQVAKLGHGLlsgeyskpvpesgdgervpeQKEVQDGIAPRM 416
           7******************************.99999999********************67766666666666655443366666****** PP
   S: 73   llkalkkeaeefsgyeqqDaqEflaflldqleeelkkeknkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvss 164
           +++ ++k ++efs+++qqDaqEf+ +l++ +e+++                             ++++  +++F++ ++++ikcl+ ++v+ 
   Q: 417  FKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNC-----------------------------RSSENPNEVFRFLVEEKIKCLATEKVKY 479
           ***********************************.............................6788899********************* PP
   S: 165  reepfldlslpvpgkeetkke...........................slqdcLesftkeeelegenkyycekckkkqeaekklklkklPev 229
           ++   + ++lpvp++   +ke                            +++cLe++ ++e++++   ++++  ++k+ a+k++++ ++P++
   Q: 480  TQRVDYIMQLPVPMDAALNKEelleyeekkrqaeeekmalpelvraqvPFSSCLEAYGAPEQVDD---FWSTALQAKSVAVKTTRFASFPDY 568
           ****************999989*****************************************97...************************ PP
   S: 230  LilhLkRfsfseeqksrekinkkvefpleLdlspfcts...................................................... 267
           L++++k+f+f  + ++++k++ ++e+p+eLd+s+   +                                                      
   Q: 569  LVIQIKKFTFGLD-WVPKKLDVSIEMPEELDISQLRGTglqpgeeelpdiapplvtpdepkgslgfygnededsfcsphfssptspmldesv 659
           **********997.*******************98888788888888888888888888888888888888777777777777777777777 PP
   S: 268  .....................................................................peekqke................ 274
                                                                                +     +                
   Q: 660  iiqlvemgfpmdacrkavyytgnsgaeaamnwvmshmddpdfanplilpgssgpgstsaaadpppedcvT-----Tivsmgfsrdqalkalr 746
           7777777777777777777777666666666666666666666666666666665555555555555550.....05555555555555566 PP
   S: 275  ...................................................kkkYeLiavvvHsG.slssGHYvayvknekgkWllfdDskV 314
                                                              + kY+L+a ++H+G s+ +GHYv+++k+ +g+W+ ++D+kV
   Q: 747  atnnsleravdwifshiddldaeaamdisegrsaadsisesvpvgpkvrdgPGKYQLFAFISHMGtSTMCGHYVCHIKK-EGRWVIYNDQKV 837
           666666667777777777777777777777777778888888888888888****************************.9*********** PP
   S: 315  tevkeeevleetgstaYvlfYkr 337
            ++  e+  +   + +Y++fY+r
   Q: 838  CAS--EKPPK---DLGYIYFYQR 855
           999..78888...89******98 PP

UBD/Alpha-Helix/UBA

   S: 4    edkleqlveMGFdreealqaLraannnleaAveyLldg 41
           ed +  +v+MGF+r++al+aLra+nn+le+Av++++++
   Q: 725  EDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSH 762
           889999******************************97 PP

Organism

Homo sapiens

Functional Description
(View)

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Protein Sequence
(Fasta)

MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF LGFGKQYVER 60
HFNKTGQRVY LHLRRTRRPK EEDPATGTGD PPRKKPTRLA IGVEGGFDLS EEKFELDEDV 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGGACTGGGA ACGGTGGGAG CCGCCGTGTG TGGAGAAGCT GCTGCCGGTG TCATGGCGGA 60
GCTGAGTGAG GAGGCGCTGC TGTCAGTATT ACCGACGATC CGGGTCCCTA AGGCTGGAGA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-1015--Disulfide bond
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0597--Phosphoprotein
KW-0645--Protease
KW-1185--Reference proteome
KW-0677--Repeat
KW-0788--Thiol protease
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR001394--Peptidase_C19_UCH
IPR015940--UBA
IPR009060--UBA-like
IPR016652--Ubiquitinyl_hydrolase
IPR018200--USP_CS
IPR028889--USP_dom
IPR013083--Znf_RING/FYVE/PHD
IPR001607--Znf_UBP

PROSITE

PS50030--UBA
PS00972--USP_1
PS00973--USP_2
PS50235--USP_3
PS50271--ZF_UBP

Pfam

PF00627--UBA
PF00443--UCH
PF02148--zf-UBP

SMART

SM00165--UBA
SM00290--ZnF_UBP

Gene Ontology

GO:0005829--C:cytosol
GO:0005764--C:lysosome
GO:0004197--F:cysteine-type endopeptidase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0036459--F:thiol-dependent ubiquitinyl hydrolase activity
GO:0043130--F:ubiquitin binding
GO:0008270--F:zinc ion binding
GO:0032436--P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0016579--P:protein deubiquitination
GO:0071108--P:protein K48-linked deubiquitination
GO:0016567--P:protein ubiquitination
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

hsa:8078

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000394	Aegilops tauschii	44.31	0.00e+00	648.00
IUUC-Aml-002104	Ailuropoda melanoleuca	97.88	0.00e+00	1640.00
IUUC-Atr-002580	Amborella trichopoda	46.31	0.00e+00	739.00
IUUC-Apl-003774	Anas platyrhynchos	91.55	0.00e+00	1527.00
IUUC-Aca-005368	Anolis carolinensis	86.13	0.00e+00	1501.00
IUUC-Aly-005727	Arabidopsis lyrata	46.93	0.00e+00	729.00
IUUC-Ath-006974	Arabidopsis thaliana	47.26	0.00e+00	731.00
IUUC-Ago-007890	Ashbya gossypii	29.59	2.00e-84	307.00
IUUC-Acl-008057	Aspergillus clavatus	33.92	1.00e-123	437.00
IUUC-Afl-008547	Aspergillus flavus	36.54	1.00e-118	420.00
IUUC-Afu-008805	Aspergillus fumigatus	34.28	3.00e-114	406.00
IUUC-Ani-009441	Aspergillus nidulans	32.35	4.00e-98	352.00
IUUC-Ang-009720	Aspergillus niger	33.64	4.00e-118	419.00
IUUC-Aor-010111	Aspergillus oryzae	34.22	2.00e-127	449.00
IUUC-Ate-010531	Aspergillus terreus	32.17	1.00e-103	370.00
IUUC-Ame-011893	Astyanax mexicanus	65.10	0.00e+00	994.00
IUUC-Bgr-012183	Blumeria graminis	33.45	6.00e-121	428.00
IUUC-Bta-013179	Bos taurus	98.11	0.00e+00	1646.00
IUUC-Bci-013858	Botrytis cinerea	34.84	3.00e-130	459.00
IUUC-Bdi-014904	Brachypodium distachyon	46.97	0.00e+00	712.00
IUUC-Bol-015296	Brassica oleracea	47.67	0.00e+00	747.00
IUUC-Bra-017939	Brassica rapa	47.79	0.00e+00	751.00
IUUC-Cel-018625	Caenorhabditis elegans	34.81	2.00e-134	473.00
IUUC-Cja-019063	Callithrix jacchus	98.00	0.00e+00	1636.00
IUUC-Cfa-020976	Canis familiaris	95.75	0.00e+00	1591.00
IUUC-Cpo-022265	Cavia porcellus	95.75	0.00e+00	1585.00
IUUC-Cre-022640	Chlamydomonas reinhardtii	39.57	6.00e-113	401.00
IUUC-Csa-024177	Chlorocebus sabaeus	96.45	0.00e+00	1471.00
IUUC-Cho-024702	Choloepus hoffmanni	89.12	0.00e+00	991.00
IUUC-Cin-025411	Ciona intestinalis	50.00	0.00e+00	828.00
IUUC-Csv-026347	Ciona savignyi	46.30	0.00e+00	791.00
IUUC-Cgl-026667	Colletotrichum gloeosporioides	34.64	2.00e-113	403.00
IUUC-Cne-026831	Cryptococcus neoformans	35.49	1.00e-128	453.00
IUUC-Dre-027982	Danio rerio	67.36	0.00e+00	1104.00
IUUC-Dno-029034	Dasypus novemcinctus	98.47	0.00e+00	1667.00
IUUC-Dor-030479	Dipodomys ordii	93.91	0.00e+00	715.00
IUUC-Dse-031467	Dothistroma septosporum	36.11	2.00e-131	463.00
IUUC-Dme-032075	Drosophila melanogaster	49.25	0.00e+00	801.00
IUUC-Ete-032865	Echinops telfairi	86.95	0.00e+00	1462.00
IUUC-Eca-034059	Equus caballus	98.71	0.00e+00	1663.00
IUUC-Eeu-035304	Erinaceus europaeus	92.44	0.00e+00	1496.00
IUUC-Fca-035515	Felis catus	98.47	0.00e+00	1651.00
IUUC-Fal-037271	Ficedula albicollis	87.81	0.00e+00	1424.00
IUUC-Fox-037745	Fusarium oxysporum	35.01	2.00e-128	453.00
IUUC-Fso-038158	Fusarium solani	35.78	6.00e-128	451.00
IUUC-Gmo-039071	Gadus morhua	65.06	0.00e+00	1075.00
IUUC-Ggr-040098	Gaeumannomyces graminis	34.14	9.00e-119	421.00
IUUC-Gga-040635	Gallus gallus	91.03	0.00e+00	1595.00
IUUC-Gac-042528	Gasterosteus aculeatus	64.30	0.00e+00	1094.00
IUUC-Gma-043614	Glycine max	46.50	0.00e+00	701.00
IUUC-Ggo-044602	Gorilla gorilla	100.00	0.00e+00	1673.00
IUUC-Hvu-047563	Hordeum vulgare	47.66	4.00e-170	591.00
IUUC-Itr-048712	Ictidomys tridecemlineatus	98.11	0.00e+00	1644.00
IUUC-Kpa-049156	Komagataella pastoris	33.06	2.00e-115	410.00
IUUC-Lch-049648	Latimeria chalumnae	81.89	0.00e+00	1375.00
IUUC-Lpe-051523	Leersia perrieri	46.77	0.00e+00	717.00
IUUC-Loc-052520	Lepisosteus oculatus	62.82	0.00e+00	1016.00
IUUC-Lma-053216	Leptosphaeria maculans	34.44	5.00e-114	406.00
IUUC-Laf-054327	Loxodonta africana	97.40	0.00e+00	1635.00
IUUC-Mcc-055443	Macaca mulatta	99.65	0.00e+00	1666.00
IUUC-Meu-056298	Macropus eugenii	93.06	0.00e+00	1059.00
IUUC-Mor-057106	Magnaporthe oryzae	35.19	4.00e-130	459.00
IUUC-Mpo-057589	Magnaporthe poae	34.80	4.00e-123	435.00
IUUC-Mtr-058680	Medicago truncatula	48.20	0.00e+00	735.00
IUUC-Mla-058903	Melampsora laricipopulina	32.80	9.00e-121	427.00
IUUC-Mga-059669	Meleagris gallopavo	90.18	0.00e+00	1512.00
IUUC-Mvi-060476	Microbotryum violaceum	34.58	2.00e-119	423.00
IUUC-Mmr-060575	Microcebus murinus	98.47	0.00e+00	1651.00
IUUC-Mdo-062183	Monodelphis domestica	91.62	0.00e+00	1529.00
IUUC-Mmu-063592	Mus musculus	98.35	0.00e+00	1649.00
IUUC-Mac-065634	Musa acuminata	43.01	0.00e+00	689.00
IUUC-Mpu-066082	Mustela putorius furo	94.92	0.00e+00	1575.00
IUUC-Mlu-067345	Myotis lucifugus	96.12	0.00e+00	1609.00
IUUC-Nfi-068558	Neosartorya fischeri	35.06	1.00e-122	434.00
IUUC-Ncr-068960	Neurospora crassa	34.88	3.00e-116	412.00
IUUC-Nle-070173	Nomascus leucogenys	97.05	0.00e+00	1609.00
IUUC-Opr-070556	Ochotona princeps	97.28	0.00e+00	1638.00
IUUC-Ont-071502	Oreochromis niloticus	65.19	0.00e+00	1090.00
IUUC-Ocu-074861	Oryctolagus cuniculus	95.28	0.00e+00	1586.00
IUUC-Oba-075109	Oryza barthii	46.49	0.00e+00	719.00
IUUC-Obr-076292	Oryza brachyantha	47.78	0.00e+00	714.00
IUUC-Ogl-077065	Oryza glaberrima	47.78	0.00e+00	749.00
IUUC-Ogu-078776	Oryza glumaepatula	46.49	0.00e+00	731.00
IUUC-Oin-080048	Oryza indica	47.31	0.00e+00	743.00
IUUC-Olo-080711	Oryza longistaminata	44.66	0.00e+00	662.00
IUUC-Ome-081506	Oryza meridionalis	45.71	0.00e+00	674.00
IUUC-Oni-082320	Oryza nivara	45.93	0.00e+00	717.00
IUUC-Opu-083801	Oryza punctata	46.67	0.00e+00	712.00
IUUC-Oru-085139	Oryza rufipogon	45.93	0.00e+00	719.00
IUUC-Osa-085713	Oryza sativa	47.90	0.00e+00	753.00
IUUC-Ola-086951	Oryzias latipes	65.65	0.00e+00	1081.00
IUUC-Olu-087822	Ostreococcus lucimarinus	41.25	5.00e-180	624.00
IUUC-Oga-088625	Otolemur garnettii	97.76	0.00e+00	1633.00
IUUC-Oar-089995	Ovis aries	95.53	0.00e+00	1613.00
IUUC-Ptr-090640	Pan troglodytes	97.17	0.00e+00	1649.00
IUUC-Pan-091974	Papio anubis	98.71	0.00e+00	1649.00
IUUC-Psi-093957	Pelodiscus sinensis	89.88	0.00e+00	1595.00
IUUC-Pma-094242	Petromyzon marinus	71.65	1.00e-164	572.00
IUUC-Pno-094835	Phaeosphaeria nodorum	34.78	1.00e-107	384.00
IUUC-Ppa-095901	Physcomitrella patens	49.37	0.00e+00	785.00
IUUC-Pfo-097148	Poecilia formosa	66.32	0.00e+00	1100.00
IUUC-Pab-098068	Pongo abelii	92.09	0.00e+00	1491.00
IUUC-Pop-098943	Populus trichocarpa	46.85	0.00e+00	716.00
IUUC-Pca-100738	Procavia capensis	97.84	0.00e+00	1134.00
IUUC-Ppe-101315	Prunus persica	47.09	0.00e+00	724.00
IUUC-Pva-102154	Pteropus vampyrus	96.46	0.00e+00	1618.00
IUUC-Pgr-103343	Puccinia graminis	33.75	2.00e-108	387.00
IUUC-Ptt-103928	Puccinia triticina	30.40	3.00e-105	376.00
IUUC-Pte-104306	Pyrenophora teres	32.16	8.00e-104	371.00
IUUC-Pyt-104696	Pyrenophora triticirepentis	33.26	9.00e-107	381.00
IUUC-Rno-105801	Rattus norvegicus	98.23	0.00e+00	1650.00
IUUC-Sce-106304	Saccharomyces cerevisiae	28.11	1.00e-71	265.00
IUUC-Sha-107635	Sarcophilus harrisii	91.74	0.00e+00	1540.00
IUUC-Sja-107678	Schizosaccharomyces japonicus	32.60	2.00e-110	394.00
IUUC-Spo-108144	Schizosaccharomyces pombe	32.15	1.00e-112	400.00
IUUC-Smo-108848	Selaginella moellendorffii	48.06	0.00e+00	739.00
IUUC-Sit-110728	Setaria italica	48.14	0.00e+00	771.00
IUUC-Sly-111523	Solanum lycopersicum	47.62	0.00e+00	762.00
IUUC-Sbi-114419	Sorghum bicolor	47.15	0.00e+00	751.00
IUUC-Sre-115225	Sporisorium reilianum	35.06	6.00e-126	445.00
IUUC-Ssc-115686	Sus scrofa	97.17	0.00e+00	1634.00
IUUC-Tgu-117346	Taeniopygia guttata	90.57	0.00e+00	1563.00
IUUC-Tru-118350	Takifugu rubripes	65.08	0.00e+00	1108.00
IUUC-Tni-120658	Tetraodon nigroviridis	65.93	0.00e+00	1093.00
IUUC-Tca-121585	Theobroma cacao	47.39	0.00e+00	720.00
IUUC-Tre-122043	Trichoderma reesei	34.30	1.00e-119	423.00
IUUC-Tvi-122303	Trichoderma virens	34.49	1.00e-125	443.00
IUUC-Tae-125686	Triticum aestivum	47.19	0.00e+00	730.00
IUUC-Tur-126732	Triticum urartu	44.72	0.00e+00	685.00
IUUC-Tme-126909	Tuber melanosporum	34.95	2.00e-119	423.00
IUUC-Tbe-127261	Tupaia belangeri	91.18	0.00e+00	1036.00
IUUC-Uma-129599	Ustilago maydis	34.40	1.00e-128	454.00
IUUC-Vda-129688	Verticillium dahliae	33.49	2.00e-119	423.00
IUUC-Vvi-131116	Vitis vinifera	47.27	0.00e+00	704.00
IUUC-Xtr-131953	Xenopus tropicalis	79.16	0.00e+00	1348.00
IUUC-Xma-133837	Xiphophorus maculatus	66.43	0.00e+00	1103.00
IUUC-Yli-134510	Yarrowia lipolytica	35.50	4.00e-130	459.00
IUUC-Zma-135786	Zea mays	47.26	0.00e+00	718.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence