iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Mmu-063710

UUCD1 version

UUC-MuM-01193

Ensembl Protein ID

ENSMUSP00000034508.7

UniProt Accession

O88623; Q6X4T9; Q6X4U1; Q8VD74; Q9JJ87; UBP2_MOUSE

Genbank Protein ID

AAQ83301.1; AAQ83302.1; AAQ83303.1; AAQ83304.1; BAA95110.1; BAE23782.1; AAH17517.1; AAC28393.1

Protein Name

Ubiquitin carboxyl-terminal hydrolase 2; 41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2

Genbank Nucleotide ID

AY255637; AY255638; AY255639; AY255640; AB041799; AK138799; BC017517; AF079565

Gene Name

Usp2; Ubp41; MNCb-0190

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000032010.15	ENSMUST00000034508.13	ENSMUSP00000034508.7
ENSMUSG00000032010.15	ENSMUST00000065461.8	ENSMUSP00000070264.7
ENSMUSG00000032010.15	ENSMUST00000176416.7	ENSMUSP00000135482.1
ENSMUSG00000032010.15	ENSMUST00000175816.1	ENSMUSP00000135788.1

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	miRTarBase	microRNA	TRANSFAC
RepTar	miRNAMap	RAID2
Protein-protein Interaction
IID	PINA	HINT	Mentha
Drug and target
GRAC
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
mUbiSiDa
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/USP	1.20e-97	328.2	282	611

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

DUB/USP

   S: 2    GlkNlgNtCYmNSvLQcLsnipelrellle.kkkeklneenpkepkgellkslqklfeelw.kskkkavsprellkalkkeaeefsgye 88
           Gl+NlgNtC+mNS+LQcLsn++elr+++l+  + ++l ++  ++ +++l+++++kl++ +w +s + +vsp+e++++++++a++f gy+
   Q: 282  GLRNLGNTCFMNSILQCLSNTRELRDYCLQrLYMRDLGHT--SSAHTALMEEFAKLIQTIWtSSPNDVVSPSEFKTQIQRYAPRFMGYN 368
           9*****************************7788888877..7899***************889999********************** PP
   S: 89   qqDaqEflaflldqleeelkk...eknkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlsl 174
           qqDaqEfl+flld+l++e+++   + + + e++ +  ++e+ ++ ++k  ++++s i dlF gqlks+++c +c+  s+ ++pf+dlsl
   Q: 369  QQDAQEFLRFLLDGLHNEVNRvaaRPKASPETLDHLPDEEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSL 457
           *******************99444222333333333344455559******************************************** PP
   S: 175  pvpgkeetkkeslqdcLesftkeeelegenkyycekckkkqeaekklklkklPevLilhLkRfsfseeqksrekinkkvefple.Ldls 262
           p+ ++    + +l dc++ ftke+ l+g++k  c +c++++++ kk++++++P++L+lhLkRf  se++ + +k+++ v+fpl+ Ldl+
   Q: 458  PIAKRGYP-EVTLMDCMRLFTKEDILDGDEKPTCCRCRARKRCIKKFSVQRFPKILVLHLKRF--SESRIRTSKLTTFVNFPLRdLDLR 543
           ****9976.779***************************************************..88899******************* PP
   S: 263  pfctspeekqkekkkYeLiavvvHsGslssGHYvayvkn.ekgkWllfdDskVtevkeeevleetgstaYvlfYk 336
           +f +++++    +  Y+L+av +HsG++ +GHY+ay+++  +g+W +f+Ds+Vt+++ ++v +   s+aY+lfY+
   Q: 544  EFASENTN----HAVYNLYAVSNHSGTTMGGHYTAYCRSpVTGEWHTFNDSSVTPMSSSQVRT---SDAYLLFYE 611
           ****9553....78*************************44666*******************...********8 PP

Organism

Mus musculus

Functional Description
(View)

Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues. Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (PubMed:23213472). Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (PubMed:25238854).

Protein Sequence
(Fasta)

MSQLSSTLKR YTESSRYTDA PYAKPGYGTY TPSSYGANLA ASFLEKEKLG FKPVSPTSFL 60
PRPRTYGPSS ILDCDRGRPL LRSDIIGSSK RSESQTRGNE RPSGSGLNGG SGFSYGVSSN 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GCTGGGTGGC GCCGATTTCC CGGGGAGGTC CTTTCTGGGC CCCCGGCGGA GGTGGGGGAG 60
AGGCGGGCAG GCACCGAGCG AGGCCAGGGA GCCCTCTGCG TCAGCCACTG CTCGCTGCGC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0025--Alternative splicing
KW-0090--Biological rhythms
KW-0131--Cell cycle
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0517--Myogenesis
KW-0539--Nucleus
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0833--Ubl conjugation pathway
KW-0862--Zinc

Interpro

IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF00443--UCH

Gene Ontology

GO:0005938--C:cell cortex
GO:0005813--C:centrosome
GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0048471--C:perinuclear region of cytoplasm
GO:0030332--F:cyclin binding
GO:0042802--F:identical protein binding
GO:0046872--F:metal ion binding
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0007049--P:cell cycle
GO:0048512--P:circadian behavior
GO:0032922--P:circadian regulation of gene expression
GO:0043153--P:entrainment of circadian clock by photoperiod
GO:0045475--P:locomotor rhythm
GO:0007517--P:muscle organ development
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045931--P:positive regulation of mitotic cell cycle
GO:0016579--P:protein deubiquitination
GO:0050821--P:protein stabilization
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

mmu:53376

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001988	Ailuropoda melanoleuca	90.95	0.00e+00	1067.00
IUUC-Aca-005132	Anolis carolinensis	60.51	0.00e+00	675.00
IUUC-Ame-011102	Astyanax mexicanus	74.49	6.00e-144	503.00
IUUC-Bta-012529	Bos taurus	88.37	0.00e+00	982.00
IUUC-Bra-016678	Brassica rapa	36.26	1.00e-46	180.00
IUUC-Cja-019016	Callithrix jacchus	86.84	0.00e+00	985.00
IUUC-Cfa-020714	Canis familiaris	90.63	0.00e+00	1073.00
IUUC-Cpo-021727	Cavia porcellus	88.05	0.00e+00	1022.00
IUUC-Csa-023071	Chlorocebus sabaeus	95.97	0.00e+00	673.00
IUUC-Cin-025797	Ciona intestinalis	50.72	1.00e-95	342.00
IUUC-Csv-026132	Ciona savignyi	52.07	3.00e-99	355.00
IUUC-Dre-027872	Danio rerio	80.94	1.00e-161	562.00
IUUC-Dno-029329	Dasypus novemcinctus	89.50	0.00e+00	1045.00
IUUC-Dor-030574	Dipodomys ordii	90.95	0.00e+00	1026.00
IUUC-Dme-032208	Drosophila melanogaster	47.21	2.00e-82	301.00
IUUC-Ete-032725	Echinops telfairi	78.64	5.00e-171	594.00
IUUC-Eca-033361	Equus caballus	91.11	0.00e+00	1070.00
IUUC-Eeu-035272	Erinaceus europaeus	76.36	0.00e+00	689.00
IUUC-Fca-036497	Felis catus	91.60	0.00e+00	1076.00
IUUC-Fal-036766	Ficedula albicollis	63.46	0.00e+00	678.00
IUUC-Gmo-039129	Gadus morhua	65.20	6.00e-122	430.00
IUUC-Gga-040889	Gallus gallus	82.84	8.00e-147	513.00
IUUC-Gac-042475	Gasterosteus aculeatus	68.39	4.00e-135	474.00
IUUC-Ggo-044814	Gorilla gorilla	90.23	6.00e-174	603.00
IUUC-Hsa-045937	Homo sapiens	87.40	0.00e+00	998.00
IUUC-Itr-048171	Ictidomys tridecemlineatus	92.57	0.00e+00	1092.00
IUUC-Lch-050527	Latimeria chalumnae	60.45	0.00e+00	632.00
IUUC-Loc-052697	Lepisosteus oculatus	78.00	3.00e-160	558.00
IUUC-Laf-054167	Loxodonta africana	84.08	0.00e+00	987.00
IUUC-Mcc-055584	Macaca mulatta	89.01	0.00e+00	1040.00
IUUC-Meu-056542	Macropus eugenii	83.71	0.00e+00	938.00
IUUC-Mga-060020	Meleagris gallopavo	59.78	0.00e+00	639.00
IUUC-Mmr-060989	Microcebus murinus	90.15	0.00e+00	1056.00
IUUC-Mdo-062761	Monodelphis domestica	82.61	0.00e+00	935.00
IUUC-Mpu-066573	Mustela putorius furo	91.11	0.00e+00	1071.00
IUUC-Nle-070013	Nomascus leucogenys	87.97	0.00e+00	972.00
IUUC-Opr-070907	Ochotona princeps	89.01	0.00e+00	1014.00
IUUC-Ont-071944	Oreochromis niloticus	80.98	1.00e-163	568.00
IUUC-Oan-073105	Ornithorhynchus anatinus	81.13	8.00e-145	506.00
IUUC-Ocu-073941	Oryctolagus cuniculus	90.63	0.00e+00	1064.00
IUUC-Ola-087412	Oryzias latipes	70.77	1.00e-151	529.00
IUUC-Oga-088973	Otolemur garnettii	90.79	0.00e+00	1065.00
IUUC-Oar-089916	Ovis aries	89.01	0.00e+00	1026.00
IUUC-Ptr-090998	Pan troglodytes	87.72	0.00e+00	1006.00
IUUC-Pan-092686	Papio anubis	89.18	0.00e+00	1042.00
IUUC-Psi-093284	Pelodiscus sinensis	69.50	2.00e-121	428.00
IUUC-Pma-094462	Petromyzon marinus	64.71	7.00e-74	269.00
IUUC-Pno-094986	Phaeosphaeria nodorum	32.11	6.00e-39	156.00
IUUC-Pfo-097352	Poecilia formosa	78.86	5.00e-166	577.00
IUUC-Pab-098688	Pongo abelii	81.42	0.00e+00	894.00
IUUC-Pca-100205	Procavia capensis	91.64	0.00e+00	636.00
IUUC-Pva-103188	Pteropus vampyrus	89.82	0.00e+00	1028.00
IUUC-Rno-105853	Rattus norvegicus	97.42	0.00e+00	1153.00
IUUC-Sha-107237	Sarcophilus harrisii	83.23	0.00e+00	934.00
IUUC-Sar-113103	Sorex araneus	90.49	0.00e+00	625.00
IUUC-Ssc-115955	Sus scrofa	91.11	0.00e+00	1040.00
IUUC-Tgu-116894	Taeniopygia guttata	67.77	3.00e-180	624.00
IUUC-Tru-117703	Takifugu rubripes	65.50	6.00e-134	470.00
IUUC-Tsy-118879	Tarsius syrichta	94.63	0.00e+00	821.00
IUUC-Tni-119866	Tetraodon nigroviridis	78.62	5.00e-153	533.00
IUUC-Tbe-127367	Tupaia belangeri	81.69	0.00e+00	652.00
IUUC-Ttr-128773	Tursiops truncatus	87.40	0.00e+00	1027.00
IUUC-Vpa-130402	Vicugna pacos	79.45	0.00e+00	761.00
IUUC-Xtr-132658	Xenopus tropicalis	57.33	2.00e-179	622.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence