iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
DNA & RNA Element
microRNA	RAID2
Protein-protein Interaction
IID	Mentha
Protein 3D Structure
PDB	MMDB
Post-translational Modifications (PTMs)
dbPAF	PHOSIDA
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	18628966

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	6.60e-132	440.0	618	947

Active Site

Position(s)	Description	Evidence
917	Glycyl thioester intermediate	{ECO:0000305}

Domain Profile

E3 activity/HECT

   S: 4    dlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldlplsksfyklll 101
           +l++rl++ f+gEegld+gGv+re+++lls+e+ np+++lf+y ++++++lq+np+s++ np+hl++F+f+Gr++++al++gr++   ++ +fyk++l
   Q: 618  ELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYV-NPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRML 714
           6899******************************************************9.89************************************ PP
   S: 102  gkklsledleevdpelykslkeikenaesdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvveyrlkegikeqveafreGfne 199
           +kkl+++d+e++dpe+y+sl ++k+n   +i+e+gl+l fsv+ e+lg++ ++eLk++Ge+++Vt+eNkeeY+ l++e+r+++gi++q++ f eGfne
   Q: 715  NKKLTIKDIETIDPEFYNSLIWVKDN---NIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNE 809
           ************************99...9******************************************************************** PP
   S: 200  viplellslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFvTgssrlPlgGfaelk....prlfvirkda 293
           v+ple+l++F+e+elel++cG++++dved++++t y++ y+++s+++ wFw++++e ++e+r+++LqFvTg++r+P+gGfael+    p++f+i+k +
   Q: 810  VVPLEWLKYFDERELELILCGMQDVDVEDWQRNTIYRH-YNRNSKQVVWFWQFVRETDNEKRARLLQFVTGTCRVPVGGFAELMgsngPQRFCIEKVG 906
           ************************************99.*********************************************************** PP
   S: 294  sddrlPssnTCfnyLkLPeYsskeilkekLlyAieetkgFe 334
           ++++lP+s+TCfn+L+LP+Y+s+++l ekL++Aieet+gF 
   Q: 907  KETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGFC 947
           ****************************************6 PP

Organism

Drosophila melanogaster

Functional Description
(View)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Notch/N signaling pathway, probably by promoting Notch ubiquitination, endocytosis and degradation. Involved in wing growth and leg joint formation.

Protein Sequence
(Fasta)

MADGNGLPAG AASGGMEAGQ TVNGAGSASP TPTSSSGAGA SGSANQGYHQ LSVTIEEASL 60
RNNGFLKPNP YVELLIDSKS KRKTDLVKNS YLPKWNEEFT VLITPNSTLH FKVLDHSSFR 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

TGTATCGAAA AACATCGTCC AAAGTTGCAG CTCTAGAAAT TGCTGCTTGC AAGCGAAGCA 60
AAATAACAAA TAAACAATTA GTGCAGCGAT ATCAACTGCT CGCGATTACG TGGACCAAAG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0217--Developmental protein
KW-0914--Notch signaling pathway
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR000008--C2_dom
IPR000569--HECT_dom
IPR001202--WW_dom

PROSITE

PS50004--C2
PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2

Pfam

PF00168--C2
PF00632--HECT
PF00397--WW

SMART

SM00239--C2
SM00119--HECTc
SM00456--WW

Gene Ontology

GO:0005938--C:cell cortex
GO:0005737--C:cytoplasm
GO:0005112--F:Notch binding
GO:0019904--F:protein domain specific binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0016348--P:imaginal disc-derived leg joint morphogenesis
GO:0008587--P:imaginal disc-derived wing margin morphogenesis
GO:0008586--P:imaginal disc-derived wing vein morphogenesis
GO:0019915--P:lipid storage
GO:0045746--P:negative regulation of Notch signaling pathway
GO:0007219--P:Notch signaling pathway
GO:0016567--P:protein ubiquitination
GO:0035209--P:pupal development
GO:0031623--P:receptor internalization
GO:0032880--P:regulation of protein localization
GO:0048190--P:wing disc dorsal/ventral pattern formation

KEGG

dme:Dmel_CG4244

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001213	Ailuropoda melanoleuca	53.80	0.00e+00	927.00
IUUC-Apl-003310	Anas platyrhynchos	71.72	0.00e+00	894.00
IUUC-Aca-005388	Anolis carolinensis	69.90	0.00e+00	887.00
IUUC-Ago-007742	Ashbya gossypii	45.09	3.00e-150	525.00
IUUC-Acl-008273	Aspergillus clavatus	46.83	8.00e-151	526.00
IUUC-Afl-008713	Aspergillus flavus	45.67	2.00e-146	512.00
IUUC-Afu-009103	Aspergillus fumigatus	47.17	5.00e-152	530.00
IUUC-Ani-009259	Aspergillus nidulans	45.20	6.00e-147	513.00
IUUC-Ang-009738	Aspergillus niger	44.37	3.00e-146	511.00
IUUC-Ate-010529	Aspergillus terreus	46.41	2.00e-149	522.00
IUUC-Ame-010601	Astyanax mexicanus	65.05	0.00e+00	830.00
IUUC-Bgr-012148	Blumeria graminis	44.48	9.00e-142	496.00
IUUC-Bta-012857	Bos taurus	70.29	0.00e+00	897.00
IUUC-Cel-018299	Caenorhabditis elegans	67.85	0.00e+00	843.00
IUUC-Cja-019448	Callithrix jacchus	71.52	0.00e+00	899.00
IUUC-Cfa-020728	Canis familiaris	70.13	0.00e+00	899.00
IUUC-Cpo-021988	Cavia porcellus	68.76	0.00e+00	892.00
IUUC-Csa-023805	Chlorocebus sabaeus	71.29	0.00e+00	885.00
IUUC-Cho-024695	Choloepus hoffmanni	63.99	0.00e+00	835.00
IUUC-Cin-025212	Ciona intestinalis	69.04	0.00e+00	860.00
IUUC-Csv-026256	Ciona savignyi	69.07	0.00e+00	845.00
IUUC-Cgl-026642	Colletotrichum gloeosporioides	44.62	2.00e-143	502.00
IUUC-Cne-026849	Cryptococcus neoformans	46.09	2.00e-150	525.00
IUUC-Dre-028245	Danio rerio	70.45	0.00e+00	907.00
IUUC-Dno-029618	Dasypus novemcinctus	71.36	0.00e+00	897.00
IUUC-Dor-030176	Dipodomys ordii	69.91	0.00e+00	871.00
IUUC-Dse-031241	Dothistroma septosporum	46.79	4.00e-149	521.00
IUUC-Ete-032779	Echinops telfairi	68.49	0.00e+00	824.00
IUUC-Eca-033313	Equus caballus	70.98	0.00e+00	889.00
IUUC-Eeu-035259	Erinaceus europaeus	63.56	0.00e+00	754.00
IUUC-Fca-035420	Felis catus	70.29	0.00e+00	900.00
IUUC-Fal-037335	Ficedula albicollis	53.97	0.00e+00	948.00
IUUC-Fox-037774	Fusarium oxysporum	45.49	7.00e-145	506.00
IUUC-Fso-038393	Fusarium solani	45.03	5.00e-142	497.00
IUUC-Gmo-038842	Gadus morhua	67.52	0.00e+00	819.00
IUUC-Ggr-040107	Gaeumannomyces graminis	44.54	1.00e-143	503.00
IUUC-Gga-040246	Gallus gallus	71.57	0.00e+00	898.00
IUUC-Gac-042211	Gasterosteus aculeatus	71.24	0.00e+00	914.00
IUUC-Ggo-045001	Gorilla gorilla	71.19	0.00e+00	896.00
IUUC-Hsa-046544	Homo sapiens	71.19	0.00e+00	897.00
IUUC-Itr-048860	Ictidomys tridecemlineatus	71.36	0.00e+00	897.00
IUUC-Kpa-049273	Komagataella pastoris	46.63	9.00e-155	540.00
IUUC-Lch-050398	Latimeria chalumnae	71.29	0.00e+00	902.00
IUUC-Loc-052116	Lepisosteus oculatus	71.03	0.00e+00	920.00
IUUC-Laf-053551	Loxodonta africana	69.72	0.00e+00	889.00
IUUC-Mcc-055655	Macaca mulatta	69.57	0.00e+00	878.00
IUUC-Meu-055953	Macropus eugenii	60.58	0.00e+00	737.00
IUUC-Mor-057097	Magnaporthe oryzae	44.65	2.00e-143	502.00
IUUC-Mla-058996	Melampsora laricipopulina	51.33	5.00e-145	507.00
IUUC-Mga-059986	Meleagris gallopavo	54.28	0.00e+00	950.00
IUUC-Mmr-060896	Microcebus murinus	70.26	0.00e+00	883.00
IUUC-Mdo-062536	Monodelphis domestica	69.49	0.00e+00	881.00
IUUC-Mmu-063202	Mus musculus	71.36	0.00e+00	894.00
IUUC-Mpu-065773	Mustela putorius furo	70.06	0.00e+00	898.00
IUUC-Mlu-067623	Myotis lucifugus	67.69	0.00e+00	835.00
IUUC-Nfi-068427	Neosartorya fischeri	47.17	8.00e-152	530.00
IUUC-Ncr-068918	Neurospora crassa	43.61	4.00e-143	501.00
IUUC-Nle-069188	Nomascus leucogenys	71.52	0.00e+00	899.00
IUUC-Opr-070965	Ochotona princeps	70.52	0.00e+00	877.00
IUUC-Ont-072226	Oreochromis niloticus	71.74	0.00e+00	922.00
IUUC-Oan-073497	Ornithorhynchus anatinus	71.33	0.00e+00	892.00
IUUC-Ocu-074271	Oryctolagus cuniculus	69.40	0.00e+00	877.00
IUUC-Ola-087438	Oryzias latipes	70.69	0.00e+00	915.00
IUUC-Oga-087922	Otolemur garnettii	70.06	0.00e+00	895.00
IUUC-Oar-089429	Ovis aries	70.13	0.00e+00	893.00
IUUC-Ptr-090484	Pan troglodytes	71.19	0.00e+00	897.00
IUUC-Pan-092117	Papio anubis	71.52	0.00e+00	899.00
IUUC-Psi-093338	Pelodiscus sinensis	54.19	0.00e+00	952.00
IUUC-Pma-094279	Petromyzon marinus	62.14	0.00e+00	710.00
IUUC-Pno-095118	Phaeosphaeria nodorum	45.95	2.00e-149	522.00
IUUC-Pfo-096171	Poecilia formosa	71.17	0.00e+00	921.00
IUUC-Pab-098654	Pongo abelii	70.02	0.00e+00	869.00
IUUC-Pca-100596	Procavia capensis	74.06	0.00e+00	775.00
IUUC-Pva-102811	Pteropus vampyrus	70.15	0.00e+00	890.00
IUUC-Pgr-103620	Puccinia graminis	50.72	5.00e-142	497.00
IUUC-Pte-104016	Pyrenophora teres	46.53	9.00e-150	523.00
IUUC-Pyt-104550	Pyrenophora triticirepentis	46.53	9.00e-150	523.00
IUUC-Rno-106125	Rattus norvegicus	71.36	0.00e+00	894.00
IUUC-Sce-106289	Saccharomyces cerevisiae	45.73	8.00e-152	530.00
IUUC-Sha-107243	Sarcophilus harrisii	69.49	0.00e+00	881.00
IUUC-Sja-107786	Schizosaccharomyces japonicus	48.95	7.00e-149	520.00
IUUC-Spo-108171	Schizosaccharomyces pombe	47.41	2.00e-154	539.00
IUUC-Sar-113761	Sorex araneus	65.19	0.00e+00	820.00
IUUC-Sre-114929	Sporisorium reilianum	44.83	6.00e-148	517.00
IUUC-Ssc-115368	Sus scrofa	70.06	0.00e+00	892.00
IUUC-Tgu-117079	Taeniopygia guttata	71.24	0.00e+00	894.00
IUUC-Tru-118062	Takifugu rubripes	69.67	0.00e+00	920.00
IUUC-Tsy-119507	Tarsius syrichta	51.45	0.00e+00	872.00
IUUC-Tni-120151	Tetraodon nigroviridis	70.03	0.00e+00	905.00
IUUC-Tvi-122595	Trichoderma virens	44.16	6.00e-141	494.00
IUUC-Tbe-127793	Tupaia belangeri	64.09	0.00e+00	774.00
IUUC-Ttr-128275	Tursiops truncatus	69.91	0.00e+00	878.00
IUUC-Vda-129753	Verticillium dahliae	44.39	1.00e-142	499.00
IUUC-Vpa-130103	Vicugna pacos	51.78	0.00e+00	671.00
IUUC-Xtr-132866	Xenopus tropicalis	67.33	0.00e+00	859.00
IUUC-Xma-133778	Xiphophorus maculatus	71.17	0.00e+00	920.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence