iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	miRTarBase	microRNA	TRANSFAC
RepTar	RAID2
Protein-protein Interaction
IID	iRefIndex	Mentha
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
PHOSIDA
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	18083927; 17934096

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	1.40e-134	449.9	585	917

Active Site

Position(s)	Description	Evidence
886	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00104}

Domain Profile

E3 activity/HECT

   S: 2    ksdlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldl 90
           ++dl++rl+v f+gEegld+gG++re+++lls+e+ np+++lf+y  +++++lq+np+s + np+hl++F f+Gr++++al++g+++d+
   Q: 585  PYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTI-NPDHLSYFCFIGRFIAMALFHGKFIDT 672
           6799*******************************************************98.89************************* PP
   S: 91   plsksfyklllgkklsledleevdpelykslkeikenaesdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvve 179
            +s +fyk++l+kkl+++dle++d e+y+sl +i++n   +iee+gl++ fsv+ eilg++++++Lk gG+ni Vt+eNk+eY+ l++e
   Q: 673  GFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDN---NIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTE 758
           ***********************************99...9************************************************ PP
   S: 180  yrlkegikeqveafreGfneviplellslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFv 268
           +r+ +g++eq++af +Gfnev+pl++l++F+e+ele+++cG++e+d+ d++++t y++ yt++s++i+wFw++++e ++e r ++LqFv
   Q: 759  WRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRH-YTRNSKQIIWFWQFVKETDNEVRMRLLQFV 846
           ********************************************************99.****************************** PP
   S: 269  TgssrlPlgGfaelk....prlfvirkdasddrlPssnTCfnyLkLPeYsskeilkekLlyAieetkgFel 335
           Tg++rlPlgGfael+    p++f+i+k ++d++lP+s+TCfn+L+LP+Y+s+e+lkekLl+Aieet+gF++
   Q: 847  TGTCRLPLGGFAELMgsngPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQ 917
           *********************************************************************96 PP

Organism

Mus musculus

Functional Description
(View)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF (By similarity). Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7.

Protein Sequence
(Fasta)

MATASPRSDT SDIHSGRLQL KVTVSSAKLK RKKNWFGTAI YTEVIVDGEV KKTAKSSSSS 60
NPKWDEQLIV NVTPQTTLEF RVWSHHTLKA DALLGKATVD LKQVLLTHNR KLEKVKEQLK 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGGTGTCGGC GCGCTTGGCG TGCGGGATCC GAGTGGCTGC GGGCGGCCTG AGCTGCCCGG 60
CCGACGCCTG CTGCGAGATG GCGATCTTGG GCGCGGAGGG CTGAGGAGGC GCCGCCGCGG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0472--Membrane
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR000008--C2_dom
IPR024928--E3_ub_ligase_SMURF1
IPR000569--HECT_dom
IPR001202--WW_dom

PROSITE

PS50004--C2
PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2

Pfam

PF00168--C2
PF00632--HECT
PF00397--WW

SMART

SM00239--C2
SM00119--HECTc
SM00456--WW

Gene Ontology

GO:0005737--C:cytoplasm
GO:0070062--C:extracellular exosome
GO:0005634--C:nucleus
GO:0005886--C:plasma membrane
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0030324--P:lung development
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0016567--P:protein ubiquitination
GO:0030217--P:T cell differentiation

KEGG

mmu:107568

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001213	Ailuropoda melanoleuca	90.67	0.00e+00	1676.00
IUUC-Apl-003310	Anas platyrhynchos	81.23	0.00e+00	1498.00
IUUC-Aca-005388	Anolis carolinensis	79.14	0.00e+00	1428.00
IUUC-Bta-012857	Bos taurus	89.48	0.00e+00	1636.00
IUUC-Cel-018299	Caenorhabditis elegans	67.36	0.00e+00	840.00
IUUC-Cja-019448	Callithrix jacchus	91.00	0.00e+00	1696.00
IUUC-Cfa-020728	Canis familiaris	91.32	0.00e+00	1669.00
IUUC-Cpo-021635	Cavia porcellus	87.23	0.00e+00	1642.00
IUUC-Csa-023805	Chlorocebus sabaeus	92.69	0.00e+00	1280.00
IUUC-Cho-024943	Choloepus hoffmanni	80.32	0.00e+00	1120.00
IUUC-Cin-025212	Ciona intestinalis	55.52	0.00e+00	956.00
IUUC-Csv-026256	Ciona savignyi	72.84	0.00e+00	902.00
IUUC-Dre-028245	Danio rerio	90.05	0.00e+00	1144.00
IUUC-Dno-029618	Dasypus novemcinctus	90.67	0.00e+00	1675.00
IUUC-Dor-030665	Dipodomys ordii	77.14	0.00e+00	1130.00
IUUC-Dme-032041	Drosophila melanogaster	71.19	0.00e+00	897.00
IUUC-Ete-032779	Echinops telfairi	95.07	0.00e+00	1115.00
IUUC-Eca-033313	Equus caballus	87.85	0.00e+00	1614.00
IUUC-Eeu-035259	Erinaceus europaeus	82.97	0.00e+00	1492.00
IUUC-Fca-035420	Felis catus	89.80	0.00e+00	1662.00
IUUC-Fal-037335	Ficedula albicollis	81.36	0.00e+00	1491.00
IUUC-Gmo-039172	Gadus morhua	86.62	0.00e+00	1050.00
IUUC-Gga-040246	Gallus gallus	81.21	0.00e+00	1488.00
IUUC-Gac-042211	Gasterosteus aculeatus	92.52	0.00e+00	1152.00
IUUC-Ggo-045001	Gorilla gorilla	90.51	0.00e+00	1492.00
IUUC-Hsa-046544	Homo sapiens	90.56	0.00e+00	1689.00
IUUC-Itr-048860	Ictidomys tridecemlineatus	91.11	0.00e+00	1662.00
IUUC-Lch-050398	Latimeria chalumnae	93.39	0.00e+00	1153.00
IUUC-Loc-052116	Lepisosteus oculatus	93.34	0.00e+00	1171.00
IUUC-Laf-053551	Loxodonta africana	88.41	0.00e+00	1625.00
IUUC-Mla-058996	Melampsora laricipopulina	45.32	3.00e-150	525.00
IUUC-Mga-059986	Meleagris gallopavo	81.12	0.00e+00	1496.00
IUUC-Mmr-060945	Microcebus murinus	87.54	0.00e+00	1618.00
IUUC-Mdo-063024	Monodelphis domestica	81.19	0.00e+00	1446.00
IUUC-Mpu-065773	Mustela putorius furo	90.37	0.00e+00	1652.00
IUUC-Mlu-067373	Myotis lucifugus	97.11	0.00e+00	649.00
IUUC-Nle-069188	Nomascus leucogenys	91.21	0.00e+00	1700.00
IUUC-Opr-070965	Ochotona princeps	86.52	0.00e+00	1591.00
IUUC-Ont-072226	Oreochromis niloticus	92.29	0.00e+00	1160.00
IUUC-Oan-073497	Ornithorhynchus anatinus	85.05	0.00e+00	1525.00
IUUC-Ocu-073891	Oryctolagus cuniculus	86.86	0.00e+00	1566.00
IUUC-Ola-087438	Oryzias latipes	91.65	0.00e+00	1162.00
IUUC-Oga-087922	Otolemur garnettii	89.94	0.00e+00	1644.00
IUUC-Oar-089317	Ovis aries	88.31	0.00e+00	1605.00
IUUC-Ptr-090484	Pan troglodytes	90.46	0.00e+00	1689.00
IUUC-Pan-092117	Papio anubis	90.89	0.00e+00	1694.00
IUUC-Psi-093338	Pelodiscus sinensis	81.49	0.00e+00	1506.00
IUUC-Pma-094279	Petromyzon marinus	75.09	0.00e+00	844.00
IUUC-Pfo-096171	Poecilia formosa	91.82	0.00e+00	1162.00
IUUC-Pab-098654	Pongo abelii	90.29	0.00e+00	1236.00
IUUC-Pva-102811	Pteropus vampyrus	78.63	0.00e+00	1387.00
IUUC-Rno-106125	Rattus norvegicus	97.39	0.00e+00	1768.00
IUUC-Sha-107243	Sarcophilus harrisii	83.93	0.00e+00	1524.00
IUUC-Sre-114929	Sporisorium reilianum	46.19	2.00e-151	530.00
IUUC-Ssc-115368	Sus scrofa	89.41	0.00e+00	1642.00
IUUC-Tgu-117079	Taeniopygia guttata	80.58	0.00e+00	1474.00
IUUC-Tru-118062	Takifugu rubripes	71.72	0.00e+00	1340.00
IUUC-Tsy-119507	Tarsius syrichta	85.47	0.00e+00	1556.00
IUUC-Tni-120151	Tetraodon nigroviridis	92.11	0.00e+00	1149.00
IUUC-Ttr-129260	Tursiops truncatus	80.15	0.00e+00	1411.00
IUUC-Uma-129332	Ustilago maydis	46.53	1.00e-145	510.00
IUUC-Vpa-130390	Vicugna pacos	78.63	0.00e+00	870.00
IUUC-Xtr-132877	Xenopus tropicalis	69.28	0.00e+00	1194.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence