iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000123594.5	ENST00000380622.4	ENSP00000369996.2
ENSG00000123594.5	ENST00000622204.1	ENSP00000479386.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
AREsite	miRTarBase	microRNA	TRANSFAC
TargetScan	miRcode	RAID2
Protein-protein Interaction
IID	iRefIndex	Mentha	InWeb_IM
Protein 3D Structure
PDB	MMDB
Disease-associated Information
GWAS Central	OMIM
Post-translational Modifications (PTMs)
dbPAF	PhosphositePlus
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
DUB/Josephin	Josephin	19699092; 21118800

Classification

Family	E-value	Score	Start	End
DUB/Josephin	4.40e-74	249.7	1	180
UBD/Alpha-Helix/UIM	0.016	16.9	224	241

Active Site

Position(s)	Description	Evidence
14	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU00331}
119	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU00331}
134	N/A	{ECO:0000255\|PROSITE-ProRule:PRU00331}

Domain Profile

DUB/Josephin

   S: 11   pesiyhekqrrelCalhalnnvlqdelfsreeldeiakrldeeerl.................qPskslldtGnydvnvisaalkvlGleav 85
           +++i+hekq+++lCa+h+lnn+lq+e+fs++el++ia++ldeeer+                 qPs++++dtG+++++vis+alk++Gle++
   Q: 1    MDFIFHEKQEGFLCAQHCLNNLLQGEYFSPVELASIAHQLDEEERMrmaeggvtseeylaflqQPSENMDDTGFFSIQVISNALKFWGLEII 92
           799***************************************************************************************** PP
   S: 86   wfdkrrelqklaldnv..lgfilnlpsslslgllklplkkqhWvtvrkvgkayynldsklkaPeligdetelrkflakllqqkyselllvvk 175
           +f++ +e+qkl++d++  ++fi+n+              kqhW+t+rk+gk+++nl+s+l++Peli+d t+l++fla+l+qq+ys  ++vvk
   Q: 93   HFNN-PEYQKLGIDPIneRSFICNY--------------KQHWFTIRKFGKHWFNLNSLLAGPELISD-TCLANFLARLQQQAYS--VFVVK 166
           ****.********************..............*****************************.****************..***** PP
   S: 176  eevedkesdqrldv 189
           ++++d+e+dq+l++
   Q: 167  GDLPDCEADQLLQI 180
           ***********996 PP

UBD/Alpha-Helix/UIM

   S: 1    dEdeeLqlAlalSlqEee 18
           +++e++q+Al+lS+qE +
   Q: 224  QDEEDFQRALELSRQETN 241
           69**************87 PP

Organism

Homo sapiens

Functional Description
(View)

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Protein Sequence
(Fasta)

MDFIFHEKQE GFLCAQHCLN NLLQGEYFSP VELASIAHQL DEEERMRMAE GGVTSEEYLA 60
FLQQPSENMD DTGFFSIQVI SNALKFWGLE IIHFNNPEYQ KLGIDPINER SFICNYKQHW 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

AAAATCCCAT GGTGTCCATT CGGTTAAAGC CCATGTTATT TCTCTCCCAG TTACCTGCCA 60
AATCTTCTGT TTAGTGCTGT GGTAAATTTC ACCTTTATTT ACTTACTAAA ACCATGCTGA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0539--Nucleus
KW-0621--Polymorphism
KW-0645--Protease
KW-1185--Reference proteome
KW-0677--Repeat
KW-0788--Thiol protease
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0833--Ubl conjugation pathway

Interpro

IPR033865--Ataxin-3
IPR006155--Josephin
IPR009068--S15_NS1_RNA-bd
IPR003903--UIM_dom

PROSITE

PS50957--JOSEPHIN
PS50330--UIM

Pfam

PF02099--Josephin

SMART

SM01246--Josephin
SM00726--UIM

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0036459--F:thiol-dependent ubiquitinyl hydrolase activity
GO:0016579--P:protein deubiquitination
GO:0006355--P:regulation of transcription, DNA-templated
GO:0006351--P:transcription, DNA-templated

KEGG

hsa:92552

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Atr-002735	Amborella trichopoda	52.08	2.00e-06	43.50
IUUC-Afu-009018	Aspergillus fumigatus	27.12	3.00e-01	30.40
IUUC-Cja-019310	Callithrix jacchus	69.86	2.00e-134	472.00
IUUC-Csa-023257	Chlorocebus sabaeus	90.70	0.00e+00	639.00
IUUC-Cme-027158	Cyanidioschyzon merolae	31.87	8.00e-16	77.00
IUUC-Mcc-054793	Macaca mulatta	85.62	3.00e-161	560.00
IUUC-Mor-057117	Magnaporthe oryzae	34.62	2.30e-01	27.30
IUUC-Nle-069430	Nomascus leucogenys	91.55	1.00e-176	612.00
IUUC-Ptr-090897	Pan troglodytes	92.68	1.00e-178	619.00
IUUC-Pan-092742	Papio anubis	91.27	0.00e+00	644.00
IUUC-Pab-098435	Pongo abelii	96.06	0.00e+00	661.00
IUUC-Tvi-122628	Trichoderma virens	40.00	1.20e+00	29.60

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence