iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-046438

UUCD1 version

UUC-HoS-01091

Ensembl Protein ID

ENSP00000302239.4

UniProt Accession

P40818; B4DKA8; Q2TB31; Q7Z3U2; Q86VA0; Q8IWI7; UBP8_HUMAN

Genbank Protein ID

BAA06225.2; BAG59120.1; CAD97662.1; AAH38801.1; AAH51345.1; AAI10591.1

Protein Name

Ubiquitin carboxyl-terminal hydrolase 8; Deubiquitinating enzyme 8; Ubiquitin isopeptidase Y; Ubiquitin thioesterase 8; Ubiquitin-specific-processing protease 8

Genbank Nucleotide ID

D29956; AK296480; BX537420; AC012170; BC051345; BC110590

Gene Name

USP8; KIAA0055; UBPY

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000138592.13	ENST00000307179.8	ENSP00000302239.4
ENSG00000138592.13	ENST00000425032.7	ENSP00000412682.3
ENSG00000138592.13	ENST00000560730.5	ENSP00000452950.1
ENSG00000138592.13	ENST00000561211.5	ENSP00000457345.1
ENSG00000138592.13	ENST00000561330.1	ENSP00000453460.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
miRWalk	RepTar	miRNAMap	miRcode
RAID2
Protein-protein Interaction
IID	PINA	HINT	Mentha
InWeb_IM
Protein 3D Structure
PDB	MMDB
Disease-associated Information
ClinVar	GWASdb	OMIM
Drug and target
TTD	CARLSBAD
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	Phospho.ELM	UniProt	PHOSIDA
BioGRID
DNA Methylation
MethyCancer	TCGA	ICGC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
DUB/USP	USP	15933713; 12704427

Classification

Family	E-value	Score	Start	End
DUB/USP	1.40e-108	364.7	777	1107

Active Site

Position(s)	Description	Evidence
786	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}
1067	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrellle.kkkeklneenpkepkgellkslqklfeelwkskkkavsprellkalkkeaeefsgyeq 89
           tGl+NlgNtCYmNS+LQcL n p l++++++  ++ ++n++n  ++kge+++++  ++++lw+++ +++sp++++ +++k++ +f gy+q
   Q: 777  TGLRNLGNTCYMNSILQCLCNAPHLADYFNRnCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKITIGKINDQFAGYSQ 866
           7*****************************9899******************************************************** PP
   S: 90   qDaqEflaflldqleeelkkeknkpkeeeeee...dkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpv 176
           qD+qE+l fl+d+l+e+l+k +n ++ +ee++   d+ ++ ++a++k+k+ n+s+i+ lFqgq+ks+++cl+c+++s ++e f++lslp+
   Q: 867  QDSQELLLFLMDGLHEDLNKADNRKRYKEENNdhlDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMYLSLPL 956
           ******************8855544444444401134566666*********************************************** PP
   S: 177  pgkeetkkeslqdcLesftkeeelegenkyycekckkkqeaekklklkklPevLilhLkRfsfseeqksrekinkkvefple.Ldlspfc 265
            + +++   +lqdcL+ f+kee+l+++n++yc++c++++++ kk++++klP+vL +hLkRfs++ + ++ +k++++v+fple Ldls+++
   Q: 957  ASTSKC---TLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGR-WK-QKLQTSVDFPLEnLDLSQYV 1041
           *****9...******************************************************996.55.6******************* PP
   S: 266  tspeekqkekkkYeLiavvvHsGslssGHYvayvknekgk.WllfdDskVtevkeeevleetgstaYvlfYk 336
           + p+++    kkY+L++v +H+G+l++GHY+ay+kn  ++ W++fdD++V+ ++ ++v++   s aY+lfY+
   Q: 1042 IGPKNN---LKKYNLFSVSNHYGGLDGGHYTAYCKNAARQrWFKFDDHEVSDISVSSVKS---SAAYILFYT 1107
           **9988...69*************************88888*******************...********8 PP

Organism

Homo sapiens

Functional Description
(View)

Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.

Protein Sequence
(Fasta)

MPAVASVPKE LYLSSSLKDL NKKTEVKPEK ISTKSYVHSA LKIFKTAEEC RLDRDEERAY 60
VLYMKYVTVY NLIKKRPDFK QQQDYFHSIL GPGNIKKAVE EAERLSESLK LRYEEAEVRK 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GCAATGCAAA TCGGGAAAAG GGGGTGAGCT GGGCTGGCTT CCGTCCTGGT AGCCAAGGCT 60
AATTCTCCCT CGAGTTCTTG GGAGATGGGC ATTTGGCGAG AAGGCTGGCG TTAGTGAAGC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0131--Cell cycle
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0967--Endosome
KW-0378--Hydrolase
KW-0472--Membrane
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-0645--Protease
KW-1185--Reference proteome
KW-0729--SH3-binding
KW-0788--Thiol protease
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR001394--Peptidase_C19_UCH
IPR001763--Rhodanese-like_dom
IPR015063--USP8_dimer
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS50206--RHODANESE_3
PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF00581--Rhodanese
PF00443--UCH
PF08969--USP8_dimer

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0043197--C:dendritic spine
GO:0005769--C:early endosome
GO:0031313--C:extrinsic component of endosome membrane
GO:0019897--C:extrinsic component of plasma membrane
GO:0030496--C:midbody
GO:0005634--C:nucleus
GO:0014069--C:postsynaptic density
GO:0045296--F:cadherin binding
GO:0004197--F:cysteine-type endopeptidase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0008283--P:cell proliferation
GO:0007032--P:endosome organization
GO:0000281--P:mitotic cytokinesis
GO:0016579--P:protein deubiquitination
GO:0071108--P:protein K48-linked deubiquitination
GO:0070536--P:protein K63-linked deubiquitination
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

hsa:9101

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001661	Ailuropoda melanoleuca	91.88	0.00e+00	1894.00
IUUC-Apl-003226	Anas platyrhynchos	69.08	0.00e+00	1442.00
IUUC-Aca-004936	Anolis carolinensis	65.54	0.00e+00	1296.00
IUUC-Ago-007959	Ashbya gossypii	35.62	1.00e-54	208.00
IUUC-Acl-008102	Aspergillus clavatus	31.32	1.00e-50	195.00
IUUC-Afl-008604	Aspergillus flavus	32.05	2.00e-49	191.00
IUUC-Afu-009001	Aspergillus fumigatus	31.39	8.00e-50	192.00
IUUC-Ani-009291	Aspergillus nidulans	31.16	1.00e-49	191.00
IUUC-Ang-009844	Aspergillus niger	32.43	8.00e-49	189.00
IUUC-Aor-010098	Aspergillus oryzae	32.05	7.00e-49	189.00
IUUC-Ate-010585	Aspergillus terreus	31.64	7.00e-49	189.00
IUUC-Ame-011029	Astyanax mexicanus	59.23	3.00e-70	258.00
IUUC-Bta-013008	Bos taurus	88.01	0.00e+00	1811.00
IUUC-Bol-015281	Brassica oleracea	38.21	1.00e-46	181.00
IUUC-Bra-017228	Brassica rapa	39.10	1.00e-48	187.00
IUUC-Cel-018482	Caenorhabditis elegans	33.82	1.00e-51	198.00
IUUC-Cja-019173	Callithrix jacchus	96.87	0.00e+00	2018.00
IUUC-Cfa-021243	Canis familiaris	91.86	0.00e+00	1897.00
IUUC-Cpo-021522	Cavia porcellus	85.60	0.00e+00	1739.00
IUUC-Cre-022582	Chlamydomonas reinhardtii	44.35	2.00e-82	299.00
IUUC-Csa-023616	Chlorocebus sabaeus	97.99	0.00e+00	1721.00
IUUC-Cho-024381	Choloepus hoffmanni	77.84	0.00e+00	1554.00
IUUC-Cin-025287	Ciona intestinalis	50.13	9.00e-99	355.00
IUUC-Csv-026253	Ciona savignyi	48.03	8.00e-87	315.00
IUUC-Cne-026821	Cryptococcus neoformans	39.89	3.00e-74	274.00
IUUC-Dre-028440	Danio rerio	68.80	0.00e+00	732.00
IUUC-Dno-029039	Dasypus novemcinctus	90.43	0.00e+00	1922.00
IUUC-Dor-031043	Dipodomys ordii	96.55	2.00e-133	470.00
IUUC-Dse-031463	Dothistroma septosporum	28.36	1.00e-41	165.00
IUUC-Dme-032089	Drosophila melanogaster	45.43	3.00e-84	306.00
IUUC-Ete-033040	Echinops telfairi	81.13	0.00e+00	1524.00
IUUC-Eca-033449	Equus caballus	92.67	0.00e+00	1911.00
IUUC-Eeu-034693	Erinaceus europaeus	96.12	8.00e-132	465.00
IUUC-Fca-035794	Felis catus	98.95	0.00e+00	931.00
IUUC-Fal-037322	Ficedula albicollis	80.69	0.00e+00	917.00
IUUC-Gmo-038584	Gadus morhua	66.94	0.00e+00	664.00
IUUC-Gga-041188	Gallus gallus	83.36	0.00e+00	935.00
IUUC-Gac-041850	Gasterosteus aculeatus	73.24	0.00e+00	745.00
IUUC-Ggo-045565	Gorilla gorilla	99.64	0.00e+00	2094.00
IUUC-Itr-048883	Ictidomys tridecemlineatus	89.53	0.00e+00	1823.00
IUUC-Kpa-049321	Komagataella pastoris	33.24	1.00e-48	187.00
IUUC-Lch-050485	Latimeria chalumnae	84.51	0.00e+00	867.00
IUUC-Loc-052769	Lepisosteus oculatus	74.95	0.00e+00	770.00
IUUC-Lma-053021	Leptosphaeria maculans	33.04	4.00e-53	203.00
IUUC-Laf-053432	Loxodonta africana	85.92	0.00e+00	1036.00
IUUC-Mcc-055823	Macaca mulatta	98.39	0.00e+00	2076.00
IUUC-Meu-056099	Macropus eugenii	97.03	8.00e-164	571.00
IUUC-Mla-059089	Melampsora laricipopulina	39.88	2.00e-71	264.00
IUUC-Mga-059739	Meleagris gallopavo	83.19	0.00e+00	930.00
IUUC-Mvi-060559	Microbotryum violaceum	40.84	9.00e-77	282.00
IUUC-Mmr-061158	Microcebus murinus	91.68	0.00e+00	1876.00
IUUC-Mdo-061801	Monodelphis domestica	90.54	0.00e+00	1019.00
IUUC-Mmu-063665	Mus musculus	81.66	0.00e+00	1671.00
IUUC-Mpu-066738	Mustela putorius furo	91.59	0.00e+00	1871.00
IUUC-Mlu-067307	Myotis lucifugus	88.39	0.00e+00	1825.00
IUUC-Nfi-068410	Neosartorya fischeri	31.39	4.00e-50	193.00
IUUC-Nle-069314	Nomascus leucogenys	99.02	0.00e+00	2077.00
IUUC-Opr-070378	Ochotona princeps	80.81	0.00e+00	1216.00
IUUC-Ont-071545	Oreochromis niloticus	76.67	0.00e+00	765.00
IUUC-Oan-073537	Ornithorhynchus anatinus	75.95	0.00e+00	1524.00
IUUC-Ocu-074193	Oryctolagus cuniculus	89.09	0.00e+00	1826.00
IUUC-Ola-086622	Oryzias latipes	57.27	0.00e+00	1083.00
IUUC-Oga-088271	Otolemur garnettii	89.46	0.00e+00	1841.00
IUUC-Oar-090431	Ovis aries	88.22	0.00e+00	1822.00
IUUC-Ptr-091339	Pan troglodytes	99.55	0.00e+00	2122.00
IUUC-Pan-091783	Papio anubis	97.52	0.00e+00	1141.00
IUUC-Psi-093187	Pelodiscus sinensis	64.03	7.00e-161	561.00
IUUC-Pma-094626	Petromyzon marinus	58.43	1.00e-141	496.00
IUUC-Pfo-096463	Poecilia formosa	75.66	0.00e+00	754.00
IUUC-Pab-097645	Pongo abelii	99.02	0.00e+00	2081.00
IUUC-Pva-103222	Pteropus vampyrus	83.33	0.00e+00	934.00
IUUC-Pgr-103408	Puccinia graminis	35.66	2.00e-53	205.00
IUUC-Ptt-103699	Puccinia triticina	45.80	1.00e-67	251.00
IUUC-Pyt-104711	Pyrenophora triticirepentis	31.11	1.00e-50	195.00
IUUC-Rno-105510	Rattus norvegicus	81.48	0.00e+00	1703.00
IUUC-Sce-106166	Saccharomyces cerevisiae	35.54	3.00e-51	197.00
IUUC-Sha-106879	Sarcophilus harrisii	89.24	0.00e+00	1007.00
IUUC-Sja-107836	Schizosaccharomyces japonicus	36.57	5.00e-48	186.00
IUUC-Sar-113019	Sorex araneus	83.54	0.00e+00	1756.00
IUUC-Sre-115031	Sporisorium reilianum	37.05	2.00e-68	254.00
IUUC-Ssc-116334	Sus scrofa	89.46	0.00e+00	1848.00
IUUC-Tgu-117062	Taeniopygia guttata	81.03	0.00e+00	920.00
IUUC-Tru-117908	Takifugu rubripes	57.50	0.00e+00	1151.00
IUUC-Tsy-118867	Tarsius syrichta	88.90	0.00e+00	1102.00
IUUC-Tni-120206	Tetraodon nigroviridis	74.85	0.00e+00	731.00
IUUC-Tme-127144	Tuber melanosporum	40.00	1.00e-78	288.00
IUUC-Tbe-127877	Tupaia belangeri	88.89	2.00e-101	364.00
IUUC-Ttr-128580	Tursiops truncatus	87.03	0.00e+00	1753.00
IUUC-Uma-129340	Ustilago maydis	35.99	5.00e-71	263.00
IUUC-Vpa-130541	Vicugna pacos	90.61	0.00e+00	1879.00
IUUC-Xtr-132161	Xenopus tropicalis	59.04	0.00e+00	1203.00
IUUC-Xma-134271	Xiphophorus maculatus	75.66	0.00e+00	753.00
IUUC-Yli-134561	Yarrowia lipolytica	36.10	5.00e-60	226.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence