iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-046473

UUCD1 version

UUC-HoS-00706

Ensembl Protein ID

ENSP00000462729.1

UniProt Accession

Q6GQQ9; B7Z643; D3DUZ8; Q5SZ60; Q8WWA7; Q9NQ53; Q9UFF4; OTU7B_HUMAN

Genbank Protein ID

CAB97494.1; BAH13129.1; CAI12651.1; EAW53586.1; EAW53587.1; AAH20622.1; AAH72681.1; CAB59268.1

Protein Name

OTU domain-containing protein 7B; Cellular zinc finger anti-NF-kappa-B protein; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne

Genbank Nucleotide ID

AJ293573; AK299790; AL590487; CH471121; CH471121; BC020622; BC072681; AL122102

Gene Name

OTUD7B; ZA20D1; CEZANNE

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000264522.5	ENST00000581312.5	ENSP00000462729.1

Annotation

Cancer Mutation
TCGA	COSMIC	CGAP	IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	TCGA	ICGC	COSMIC
THPA	HPM	SZDB
DNA & RNA Element
UTRdb	circBase	circRNADb	CircNet
miRTarBase	microRNA	TRANSFAC	RepTar
SomamiR	miRcode	RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Protein 3D Structure
PDB
Disease-associated Information
GWASdb	GWAS Central	OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	Phospho.ELM	UniProt	PHOSIDA
BioGRID	mUbiSiDa
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
UBD/ZnF/ZnF_A20	ZnF_A20	23827681

Classification

Family	E-value	Score	Start	End
DUB/OTU	6.60e-37	128.7	184	365
UBD/ZnF/ZnF_A20	3.10e-16	60.7	796	830

Active Site

Position(s)	Description	Evidence
191	N/A	{ECO:0000250}
194	Nucleophile	{ECO:0000269\|PubMed:12682062, ECO:0000269\|PubMed:14748687, ECO:0000269\|PubMed:22179831, ECO:0000269\|PubMed:27732584}
358	Proton acceptor	{ECO:0000269\|PubMed:27732584}

Domain Profile

DUB/OTU

   S: 2    lilktkgDGnCLlrAvsqsllgvfddds.lsdalveaLreltseylqkesdffsefieek...........edekefcqklvepe....... 74
           l+l+t+gDGnCLl+A+s +++g++d+d+ l++al+  + + +++++ k+++++++++++k           ++e++++ kl+++e       
   Q: 184  LPLATTGDGNCLLHAASLGMWGFHDRDLmLRKALYALMEKGVEKEALKRRWRWQQTQQNKesglvytedewQKEWNELIKLASSEprmhlgt 275
           6899********************************************************************************98887765 PP
   S: 75   akg.................eeihifaLaqiLsrpiivvdvdsles.........kvggillpllfpeek.lkkpivLlYrsgHYdaLval 138
             g                 ee+h+f+La++L+rpi+vv++++l++         ++ggi+lpl++p+++ +++p+vL+Y+++H++aLv++
   Q: 276  N-GancggvesseepvyeslEEFHVFVLAHVLRRPIVVVADTMLRDsggeafapiPFGGIYLPLEVPASQcHRSPLVLAYDQAHFSALVSM 365
           0.255579********************************************************************************986 PP

UBD/ZnF/ZnF_A20

   S: 1    dpskllCkk.gCgfYGnpetngyCSkCyreelrrr 34
           +p++++Ck+ +C+fYG+petn++CS+Cyreelrrr
   Q: 796  PPTQTKCKQpNCSFYGHPETNNFCSCCYREELRRR 830
           59********************************9 PP

Organism

Homo sapiens

Functional Description
(View)

Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections (By similarity). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (By similarity). Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin.

Protein Sequence
(Fasta)

MTLDMDAVLS DFVRSTGAEP GLARDLLEGK NWDVNAALSD FEQLRQVHAG NLPPSFSEGS 60
GGSRTPEKGF SDREPTRPPR PILQRQDDIV QEKRLSRGIS HASSSIVSLA RSHVSSNGGG 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGGCAAAAAG CCACTGGGGG AGGCCAGTCC CGATCAGTCC TTTCTGGCTC CAGCTCCAGA 60
TCCCGTGCGG AGCAACAGCT GCGGTCGCAG TCTCCACCAC CGCCTCCACC TCCATCGCCG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-1064--Adaptive immunity
KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0378--Hydrolase
KW-0391--Immunity
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR003323--OTU_dom
IPR002653--Znf_A20

PROSITE

PS50802--OTU
PS51036--ZF_A20

Pfam

PF02338--OTU
PF01754--zf-A20

SMART

SM00259--ZnF_A20

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0008234--F:cysteine-type peptidase activity
GO:0003677--F:DNA binding
GO:0070530--F:K63-linked polyubiquitin binding
GO:1990380--F:Lys48-specific deubiquitinase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0008270--F:zinc ion binding
GO:0002250--P:adaptive immune response
GO:0006955--P:immune response
GO:0002385--P:mucosal immune response
GO:0043124--P:negative regulation of I-kappaB kinase/NF-kappaB signaling
GO:0032717--P:negative regulation of interleukin-8 production
GO:1900181--P:negative regulation of protein localization to nucleus
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0016579--P:protein deubiquitination
GO:0071947--P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0035871--P:protein K11-linked deubiquitination
GO:0071108--P:protein K48-linked deubiquitination
GO:0070536--P:protein K63-linked deubiquitination

KEGG

hsa:56957

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002119	Ailuropoda melanoleuca	93.32	0.00e+00	1419.00
IUUC-Apl-003567	Anas platyrhynchos	67.82	0.00e+00	968.00
IUUC-Bta-013398	Bos taurus	93.71	0.00e+00	1383.00
IUUC-Cel-018468	Caenorhabditis elegans	50.67	2.00e-85	310.00
IUUC-Cja-019965	Callithrix jacchus	96.21	0.00e+00	1438.00
IUUC-Cfa-020191	Canis familiaris	93.36	0.00e+00	1421.00
IUUC-Cpo-022232	Cavia porcellus	90.46	0.00e+00	1333.00
IUUC-Csa-023593	Chlorocebus sabaeus	98.46	0.00e+00	1551.00
IUUC-Cho-024729	Choloepus hoffmanni	84.27	9.00e-171	593.00
IUUC-Csv-026177	Ciona savignyi	30.56	4.00e-03	35.00
IUUC-Dno-029900	Dasypus novemcinctus	92.83	0.00e+00	1362.00
IUUC-Dor-031121	Dipodomys ordii	85.38	0.00e+00	692.00
IUUC-Ete-032473	Echinops telfairi	88.99	0.00e+00	1040.00
IUUC-Eca-033946	Equus caballus	95.17	0.00e+00	1050.00
IUUC-Eeu-034670	Erinaceus europaeus	91.34	0.00e+00	1356.00
IUUC-Fca-035871	Felis catus	93.95	0.00e+00	1350.00
IUUC-Fso-038203	Fusarium solani	29.59	6.00e-02	33.50
IUUC-Gmo-039373	Gadus morhua	77.25	0.00e+00	682.00
IUUC-Gga-041105	Gallus gallus	67.72	0.00e+00	990.00
IUUC-Gac-041806	Gasterosteus aculeatus	77.78	0.00e+00	728.00
IUUC-Ggo-045472	Gorilla gorilla	99.76	0.00e+00	1523.00
IUUC-Itr-048841	Ictidomys tridecemlineatus	94.66	0.00e+00	1423.00
IUUC-Lch-050432	Latimeria chalumnae	63.59	0.00e+00	956.00
IUUC-Mcc-055261	Macaca mulatta	98.10	0.00e+00	1550.00
IUUC-Meu-056477	Macropus eugenii	79.45	0.00e+00	942.00
IUUC-Mga-059533	Meleagris gallopavo	69.42	0.00e+00	991.00
IUUC-Mdo-062847	Monodelphis domestica	82.82	0.00e+00	1169.00
IUUC-Mmu-064260	Mus musculus	88.98	0.00e+00	1319.00
IUUC-Mpu-066876	Mustela putorius furo	88.52	0.00e+00	1304.00
IUUC-Mlu-068039	Myotis lucifugus	93.72	0.00e+00	1396.00
IUUC-Nle-069260	Nomascus leucogenys	99.05	0.00e+00	1523.00
IUUC-Opr-070564	Ochotona princeps	91.61	0.00e+00	785.00
IUUC-Ocu-073824	Oryctolagus cuniculus	95.19	0.00e+00	954.00
IUUC-Opu-083273	Oryza punctata	24.36	5.80e-02	32.70
IUUC-Oga-088124	Otolemur garnettii	93.96	0.00e+00	1392.00
IUUC-Oar-090086	Ovis aries	94.31	0.00e+00	1389.00
IUUC-Ptr-091622	Pan troglodytes	99.84	0.00e+00	1149.00
IUUC-Pan-092724	Papio anubis	97.98	0.00e+00	1540.00
IUUC-Psi-093333	Pelodiscus sinensis	64.59	0.00e+00	957.00
IUUC-Pab-098667	Pongo abelii	99.17	0.00e+00	1502.00
IUUC-Pca-100701	Procavia capensis	92.34	0.00e+00	740.00
IUUC-Pva-102136	Pteropus vampyrus	92.29	0.00e+00	1369.00
IUUC-Rno-105546	Rattus norvegicus	89.81	0.00e+00	1338.00
IUUC-Sha-106732	Sarcophilus harrisii	82.60	0.00e+00	1077.00
IUUC-Sar-113562	Sorex araneus	81.40	0.00e+00	1106.00
IUUC-Ssc-116191	Sus scrofa	94.19	0.00e+00	1405.00
IUUC-Tgu-116942	Taeniopygia guttata	75.89	0.00e+00	783.00
IUUC-Tsy-119343	Tarsius syrichta	84.30	0.00e+00	1220.00
IUUC-Tur-126860	Triticum urartu	24.39	3.70e-02	33.10
IUUC-Tbe-127283	Tupaia belangeri	94.55	0.00e+00	755.00
IUUC-Ttr-128692	Tursiops truncatus	90.51	0.00e+00	1300.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence