iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Mmu-063146

UUCD1 version

UUC-MuM-01032

Ensembl Protein ID

ENSMUSP00000040431.8

UniProt Accession

Q9WUD1; Q9DCJ0; CHIP_MOUSE

Genbank Protein ID

AAD33401.1; BAB22329.1; BAB23315.1; BAC32489.1; BAE38905.1; AAH27427.1; AAH38939.1

Protein Name

STIP1 homology and U box-containing protein 1; Carboxy terminus of Hsp70-interacting protein; E3 ubiquitin-protein ligase CHIP; RING-type E3 ubiquitin transferase CHIP

Genbank Nucleotide ID

AF129086; AK002752; AK004464; AK045776; AK166630; BC027427; BC038939

Gene Name

Stub1; Chip

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000039615.8	ENSMUST00000044911.8	ENSMUSP00000040431.8

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
microRNA	TRANSFAC	RepTar	RAID2
Protein-protein Interaction
IID	iRefIndex	HINT	Mentha
Protein 3D Structure
PDB	MMDB
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
Phospho.ELM	UniProt	PHOSIDA
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/RING/U-box	U-box	23973223

Classification

Family	E-value	Score	Start	End
E3 activity/RING/U-box	1.90e-26	93.0	227	300

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/U-box

   S: 1    evpeeflCpIslelMrdPvvlpsGitydrssIekwlasggktcPvtrqplteedlvPnltlrrliqawceenss 74
           ++p+++++ Is+elMr+P+++psGitydr+ Ie++l++ g+ +Pvtr+plt+e+l+Pnl+++++i+a+++en++
   Q: 227  DIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGW 300
           69********************************************************************9986 PP

Organism

Mus musculus

Functional Description
(View)

E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner (PubMed:23973223). Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and promotes ubiquitin-mediated protein degradation. Negatively regulates TGF-beta signaling by modulating the basal level of SMAD3 via ubiquitin-mediated degradation (By similarity).

Protein Sequence
(Fasta)

MKGKEEKEGG ARLGTGGGGS PDKSPSAQEL KEQGNRLFVG RKYPEAAACY GRAITRNPLV 60
AVYYTNRALC YLKMQQPEQA LADCRRALEL DGQSVKAHFF LGQCQLEMES YDEAIANLQR 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGCCGCAGGC AGCTCCTCCC GATTCCCGAG GAAGCCCCTC GAGAGCGGTC TGATCTGCGG 60
GCCTCTCTTC TACCCTCAAT TCCGCCTTCC TACCGGCGGT CCAGACGTTC CCAGGCTGTC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0227--DNA damage
KW-0234--DNA repair
KW-1017--Isopeptide bond
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0802--TPR repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR023114--Elongated_TPR_rpt_dom
IPR013026--TPR-contain_dom
IPR011990--TPR-like_helical_dom
IPR019734--TPR_repeat
IPR003613--Ubox_domain
IPR013083--Znf_RING/FYVE/PHD

PROSITE

PS50005--TPR
PS50293--TPR_REGION
PS51698--U_BOX

Pfam

PF04564--U-box

SMART

SM00028--TPR
SM00504--Ubox

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005783--C:endoplasmic reticulum
GO:0070062--C:extracellular exosome
GO:0042405--C:nuclear inclusion body
GO:0005654--C:nucleoplasm
GO:0031371--C:ubiquitin conjugating enzyme complex
GO:0000151--C:ubiquitin ligase complex
GO:0030018--C:Z disc
GO:0019899--F:enzyme binding
GO:0001664--F:G-protein coupled receptor binding
GO:0031072--F:heat shock protein binding
GO:0030544--F:Hsp70 protein binding
GO:0051879--F:Hsp90 protein binding
GO:0019900--F:kinase binding
GO:0051787--F:misfolded protein binding
GO:0030674--F:protein binding, bridging
GO:0042803--F:protein homodimerization activity
GO:0046332--F:SMAD binding
GO:0030911--F:TPR domain binding
GO:0061630--F:ubiquitin protein ligase activity
GO:1904264--F:ubiquitin protein ligase activity involved in ERAD pathway
GO:0031625--F:ubiquitin protein ligase binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0034450--F:ubiquitin-ubiquitin ligase activity
GO:0071218--P:cellular response to misfolded protein
GO:0006281--P:DNA repair
GO:0030968--P:endoplasmic reticulum unfolded protein response
GO:0006515--P:misfolded or incompletely synthesized protein catabolic process
GO:0032091--P:negative regulation of protein binding
GO:0030512--P:negative regulation of transforming growth factor beta receptor signaling pathway
GO:0090035--P:positive regulation of chaperone-mediated protein complex assembly
GO:0032436--P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0031398--P:positive regulation of protein ubiquitination
GO:0051443--P:positive regulation of ubiquitin-protein transferase activity
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0051865--P:protein autoubiquitination
GO:0006457--P:protein folding
GO:0070534--P:protein K63-linked ubiquitination
GO:0051604--P:protein maturation
GO:0000209--P:protein polyubiquitination
GO:0016567--P:protein ubiquitination
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0031943--P:regulation of glucocorticoid metabolic process
GO:0031647--P:regulation of protein stability
GO:0006511--P:ubiquitin-dependent protein catabolic process
GO:0030579--P:ubiquitin-dependent SMAD protein catabolic process

KEGG

mmu:56424

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000537	Aegilops tauschii	35.71	1.00e-38	153.00
IUUC-Aml-001350	Ailuropoda melanoleuca	97.60	5.00e-140	489.00
IUUC-Atr-003168	Amborella trichopoda	35.38	1.00e-30	125.00
IUUC-Apl-003736	Anas platyrhynchos	87.65	9.00e-126	442.00
IUUC-Aca-004751	Anolis carolinensis	85.21	2.00e-136	478.00
IUUC-Aly-006292	Arabidopsis lyrata	33.33	1.00e-43	169.00
IUUC-Ath-007012	Arabidopsis thaliana	32.38	7.00e-41	160.00
IUUC-Acl-008025	Aspergillus clavatus	32.75	9.00e-33	133.00
IUUC-Afl-008721	Aspergillus flavus	31.47	4.00e-31	128.00
IUUC-Afu-008853	Aspergillus fumigatus	33.10	1.00e-32	133.00
IUUC-Ani-009349	Aspergillus nidulans	31.85	6.00e-32	130.00
IUUC-Ang-009674	Aspergillus niger	33.33	2.00e-29	122.00
IUUC-Aor-010014	Aspergillus oryzae	29.89	1.00e-25	109.00
IUUC-Ate-010467	Aspergillus terreus	30.85	1.00e-26	113.00
IUUC-Ame-011215	Astyanax mexicanus	58.64	2.00e-87	315.00
IUUC-Bgr-011971	Blumeria graminis	29.39	5.00e-24	104.00
IUUC-Bta-013025	Bos taurus	96.03	4.00e-160	556.00
IUUC-Bci-013945	Botrytis cinerea	39.51	6.00e-15	74.70
IUUC-Bdi-014483	Brachypodium distachyon	38.41	3.00e-44	171.00
IUUC-Bol-015676	Brassica oleracea	35.40	5.00e-45	175.00
IUUC-Bra-017897	Brassica rapa	32.54	2.00e-28	119.00
IUUC-Cel-018323	Caenorhabditis elegans	40.79	4.00e-54	204.00
IUUC-Cja-019657	Callithrix jacchus	96.84	1.00e-160	558.00
IUUC-Cfa-020470	Canis familiaris	96.97	1.00e-127	448.00
IUUC-Cpo-022033	Cavia porcellus	97.37	2.00e-165	574.00
IUUC-Cre-022896	Chlamydomonas reinhardtii	27.59	3.00e-28	118.00
IUUC-Csa-023038	Chlorocebus sabaeus	97.89	6.00e-159	553.00
IUUC-Cin-025381	Ciona intestinalis	53.02	4.00e-85	307.00
IUUC-Cgl-026524	Colletotrichum gloeosporioides	32.26	4.00e-38	151.00
IUUC-Dre-027610	Danio rerio	78.95	4.00e-131	460.00
IUUC-Dno-030087	Dasypus novemcinctus	94.41	3.00e-161	560.00
IUUC-Dor-031029	Dipodomys ordii	91.93	5.00e-151	526.00
IUUC-Dse-031275	Dothistroma septosporum	32.31	2.00e-25	109.00
IUUC-Dme-031856	Drosophila melanogaster	57.71	7.00e-93	333.00
IUUC-Ete-033087	Echinops telfairi	91.21	4.00e-131	460.00
IUUC-Eca-033512	Equus caballus	97.60	3.00e-140	490.00
IUUC-Eeu-034763	Erinaceus europaeus	90.76	4.00e-91	326.00
IUUC-Fca-036460	Felis catus	97.43	3.00e-153	533.00
IUUC-Fal-037441	Ficedula albicollis	87.06	2.00e-143	501.00
IUUC-Fox-038028	Fusarium oxysporum	30.72	1.00e-36	146.00
IUUC-Fso-038323	Fusarium solani	31.91	5.00e-28	117.00
IUUC-Gmo-038511	Gadus morhua	76.84	7.00e-125	439.00
IUUC-Ggr-039964	Gaeumannomyces graminis	31.25	2.00e-25	109.00
IUUC-Gga-040854	Gallus gallus	87.41	1.00e-142	499.00
IUUC-Gac-042175	Gasterosteus aculeatus	76.14	2.00e-121	427.00
IUUC-Gma-044046	Glycine max	35.14	5.00e-42	164.00
IUUC-Ggo-044512	Gorilla gorilla	97.89	6.00e-159	553.00
IUUC-Hsa-046872	Homo sapiens	97.89	6.00e-159	553.00
IUUC-Hvu-047661	Hordeum vulgare	34.19	7.00e-39	154.00
IUUC-Itr-048871	Ictidomys tridecemlineatus	97.89	9.00e-163	565.00
IUUC-Lch-049988	Latimeria chalumnae	81.82	3.00e-131	460.00
IUUC-Lpe-050693	Leersia perrieri	34.42	3.00e-42	164.00
IUUC-Loc-052195	Lepisosteus oculatus	78.04	5.00e-112	397.00
IUUC-Lma-053207	Leptosphaeria maculans	30.29	3.00e-27	117.00
IUUC-Laf-054584	Loxodonta africana	94.08	2.00e-160	557.00
IUUC-Mcc-054689	Macaca mulatta	97.89	6.00e-159	553.00
IUUC-Meu-055998	Macropus eugenii	88.95	2.00e-80	292.00
IUUC-Mor-057116	Magnaporthe oryzae	34.04	1.00e-39	156.00
IUUC-Mtr-057684	Medicago truncatula	31.79	5.00e-38	151.00
IUUC-Mga-059332	Meleagris gallopavo	87.65	1.00e-125	441.00
IUUC-Mmr-061377	Microcebus murinus	98.25	2.00e-159	554.00
IUUC-Mdo-061963	Monodelphis domestica	90.56	8.00e-145	506.00
IUUC-Mac-064932	Musa acuminata	33.66	5.00e-27	114.00
IUUC-Mpu-066418	Mustela putorius furo	97.54	3.00e-161	560.00
IUUC-Mlu-067066	Myotis lucifugus	96.05	2.00e-162	565.00
IUUC-Nfi-068601	Neosartorya fischeri	33.10	2.00e-32	132.00
IUUC-Ncr-068761	Neurospora crassa	41.67	4.00e-17	82.00
IUUC-Nle-069374	Nomascus leucogenys	97.89	6.00e-159	553.00
IUUC-Opr-070765	Ochotona princeps	92.90	5.00e-97	346.00
IUUC-Ont-071996	Oreochromis niloticus	79.30	6.00e-129	453.00
IUUC-Oba-075967	Oryza barthii	35.02	1.00e-42	166.00
IUUC-Obr-076440	Oryza brachyantha	34.84	2.00e-30	129.00
IUUC-Ogl-076959	Oryza glaberrima	35.53	6.00e-43	167.00
IUUC-Ogu-078247	Oryza glumaepatula	35.36	1.00e-39	156.00
IUUC-Oin-079616	Oryza indica	35.53	6.00e-43	167.00
IUUC-Olo-081083	Oryza longistaminata	29.23	8.00e-29	120.00
IUUC-Ome-081935	Oryza meridionalis	29.06	1.00e-20	95.90
IUUC-Oni-082535	Oryza nivara	35.36	1.00e-39	156.00
IUUC-Opu-083794	Oryza punctata	33.90	3.00e-42	165.00
IUUC-Oru-085153	Oryza rufipogon	34.78	1.00e-42	166.00
IUUC-Osa-085936	Oryza sativa	35.82	2.00e-16	79.00
IUUC-Ola-087081	Oryzias latipes	77.89	8.00e-129	452.00
IUUC-Olu-087790	Ostreococcus lucimarinus	31.18	8.00e-31	127.00
IUUC-Oga-088331	Otolemur garnettii	97.04	1.00e-161	562.00
IUUC-Oar-089987	Ovis aries	91.80	2.00e-134	471.00
IUUC-Ptr-090855	Pan troglodytes	97.89	6.00e-159	553.00
IUUC-Pan-092801	Papio anubis	92.28	1.00e-153	535.00
IUUC-Psi-093393	Pelodiscus sinensis	86.45	1.00e-124	439.00
IUUC-Pma-094680	Petromyzon marinus	68.93	2.00e-109	388.00
IUUC-Pno-094913	Phaeosphaeria nodorum	32.77	4.00e-33	135.00
IUUC-Ppa-095508	Physcomitrella patens	31.95	7.00e-41	160.00
IUUC-Pfo-096850	Poecilia formosa	77.54	3.00e-126	444.00
IUUC-Pab-097963	Pongo abelii	97.89	6.00e-159	553.00
IUUC-Pop-098805	Populus trichocarpa	42.86	1.00e-24	106.00
IUUC-Pca-100788	Procavia capensis	95.07	3.00e-162	563.00
IUUC-Ppe-101221	Prunus persica	33.81	6.00e-44	171.00
IUUC-Pva-103076	Pteropus vampyrus	95.39	5.00e-162	563.00
IUUC-Pte-104237	Pyrenophora teres	31.94	5.00e-32	131.00
IUUC-Pyt-104468	Pyrenophora triticirepentis	31.47	4.00e-32	131.00
IUUC-Rno-105204	Rattus norvegicus	99.34	2.00e-164	571.00
IUUC-Sha-106579	Sarcophilus harrisii	85.94	9.00e-125	439.00
IUUC-Smo-109525	Selaginella moellendorffii	35.77	7.00e-41	160.00
IUUC-Sit-110072	Setaria italica	35.02	5.00e-41	160.00
IUUC-Sly-111473	Solanum lycopersicum	35.59	1.00e-43	169.00
IUUC-Stu-112353	Solanum tuberosum	34.98	7.00e-42	164.00
IUUC-Sbi-114250	Sorghum bicolor	35.19	1.00e-43	169.00
IUUC-Ssc-115416	Sus scrofa	96.38	1.00e-162	565.00
IUUC-Tgu-117272	Taeniopygia guttata	93.52	1.00e-56	211.00
IUUC-Tru-117777	Takifugu rubripes	78.25	2.00e-127	447.00
IUUC-Tni-120572	Tetraodon nigroviridis	79.65	1.00e-128	452.00
IUUC-Tca-121149	Theobroma cacao	37.05	3.00e-47	181.00
IUUC-Tre-122230	Trichoderma reesei	33.21	4.00e-32	131.00
IUUC-Tvi-122678	Trichoderma virens	35.25	4.00e-40	158.00
IUUC-Tae-122986	Triticum aestivum	34.56	3.00e-42	165.00
IUUC-Tur-126009	Triticum urartu	34.40	5.00e-38	151.00
IUUC-Tme-126948	Tuber melanosporum	35.84	1.00e-40	159.00
IUUC-Ttr-129031	Tursiops truncatus	93.75	6.00e-160	556.00
IUUC-Vda-130023	Verticillium dahliae	31.90	1.00e-37	149.00
IUUC-Vvi-131091	Vitis vinifera	49.44	2.00e-25	108.00
IUUC-Xtr-132871	Xenopus tropicalis	82.86	5.00e-129	453.00
IUUC-Xma-134220	Xiphophorus maculatus	77.89	1.00e-126	446.00
IUUC-Yli-134462	Yarrowia lipolytica	28.27	5.00e-28	117.00
IUUC-Zma-135773	Zea mays	36.63	6.00e-45	174.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence