PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus RSPO1 RNF43 23756651 biogrid exp P P P P P P P G P P P P P P P P P P P G UBE2D1 RNF43 19690564 biogrid,intact exp P G P P GP GP G P P G G P GP GP G GP GP G P G P GP GP G RNF43 KDM5B 19336002 biogrid exp P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G UBB RNF43 18313049 intact exp P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G RNF43 HECW1 21108931 biogrid exp P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P P GP G UBE2E1 RNF43 19690564 biogrid,intact exp P GP P GP GP G P G GP P GP G G GP G G P GP GP GP G RNF43 RNF43 18313049 biogrid,intact exp P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G DVL2 RNF43 25891077 biogrid exp P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G TP53 RNF43 21108931 biogrid exp G G G G G GP G G G GP G G GP G GP G UBE2N RNF43 19690564 biogrid,intact exp P GP P P P GP GP G P P G GP P G GP G GP GP GP P GP P GP GP G RNF43 UBE2D4 19690564 biogrid,intact exp P G P P GP GP G P P G G P GP GP G GP GP G P G P GP GP G RNF43 UBE2E3 19690564 biogrid,intact exp P GP P GP GP G P G GP P GP G G GP G G P GP GP GP G UBE2D2 RNF43 18313049,19690564, 25402006 biogrid,intact exp, pred P G P P GP GP G P P G G P GP GP G GP GP G P G P GP GP G UBE2D3 RNF43 18313049,19690564, 25402006 biogrid,intact exp, pred P G P P GP GP G P P G G P GP GP G GP GP G P G P GP GP G SFPQ RNF43 18655028, 25402006 biogrid,intact exp, pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G NONO RNF43 18655028, 25402006 biogrid,intact exp, pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G RNF43 AKAP8L 18313049, 25402006 biogrid,intact exp, pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G FZD5 RNF43 ortho P GP P P G G G G GP P G GP G GP GP GP GP GP P G RAD23B RNF43 ortho P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G NEDD4 RNF43 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP P G RNF43 RFWD3 25402006 pred G G G G G G G G G G G G XIAP RNF43 25402006 pred P GP P P GP GP G P P G GP P G GP G G GP GP GP GP GP G RNF43 PSPC1 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G DHX9 RNF43 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G RNF43 RNF26 25402006 pred P GP P P P GP GP G P P G GP P G GP G GP GP G GP P GP GP G RNF43 DZIP3 25402006 pred P GP P P P GP GP G P P G GP P GP GP G P GP GP P GP GP GP G TMPO RNF43 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G CD2BP2 RNF43 25402006 pred P GP P P P GP GP P P G GP P G GP G GP GP GP GP P GP GP G RNF43 AMFR 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G RNF43 RNF11 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G RNF43 RNF25 25402006 pred P P P P P P P P P P P P P P P P RNF43 ZNRF1 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G SNRPA RNF43 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G RNF43 TBK1 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G RELA RNF43 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G RNF4 RNF43 25402006 pred P G P P GP GP G G G P GP G G GP GP GP P G GP GP G RNF43 DTX3L 25402006 pred P GP P P P GP GP G P P G GP P GP G G GP G GP P GP P GP GP G RNF43 SIAH1 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G ARRB2 RNF43 25402006 pred G G G G G G G G G G G G G G RNF43 TRAF6 25402006 pred P GP P P GP GP G P G GP GP G G GP GP GP GP P GP GP G RNF43 RNF5 25402006 pred P GP P P GP GP G P P G GP P G GP G GP GP GP P GP GP GP G RNF43 RNF111 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G RELB RNF43 25402006 pred P GP P P GP GP G P P G GP P G G G G G G GP P GP GP G CRTC2 RNF43 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G TRAF1 RNF43 25402006 pred G P P GP GP G P G G P G G G G G G P G G G TOP1 RNF43 25402006 pred P GP P P P GP GP G P P G GP P GP GP G GP GP GP P GP P GP GP G PITX3 RNF43 25402006 pred P P P P P P P P P P P P P P P P P

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q68DV7 Q76N89 two hybrid 21108931 direct interaction X 2 Q68DV7 Q76N89 affinity chromatography technology 21108931 physical association X 2 Q68DV7 P04637-7 affinity chromatography technology 21108931 physical association X 2 Q68DV7 P04637-7 affinity chromatography technology 21108931 physical association X 2 Q68DV7 Q15233-2 affinity chromatography technology 18655028 physical association X 2 Q68DV7 Q15233-2 affinity chromatography technology 18655028 physical association X 2 Q68DV7 A0A087WW00 two hybrid 19690564 direct interaction X 2 Q68DV7 P62837 two hybrid 19690564 direct interaction X 2 Q68DV7 P61077-3 two hybrid 19690564 direct interaction X 2 Q68DV7 A0A024RA90 two hybrid 19690564 direct interaction X 2 Q68DV7 A0A024R2K3 two hybrid 19690564 direct interaction X 2 Q68DV7 P61088 two hybrid 19690564 direct interaction X 2 Q969T4 Q68DV7 two hybrid 19690564 direct interaction X 2 Q9ULX6 Q68DV7 two hybrid 18313049 direct interaction X 2 Q9ULX6 Q68DV7 affinity chromatography technology 18313049 physical association X 2 Q68DV7 P23246 affinity chromatography technology 18655028 physical association X 2 Q68DV7 P23246 affinity chromatography technology 18655028 physical association X 2 Q68DV7 Q2MKA7-2 pull down 23756651 direct interaction X 2 Q68DV7 P0CG48 affinity chromatography technology 18313049 physical association X 2 Q68DV7 Q9UGL1 affinity chromatography technology 19336002 physical association X 2 Q68DV7 enzymatic study 18313049 ubiquitination reaction C 4 Q68DV7 enzymatic study 18313049 ubiquitination reaction C 4 Q68DV7 P0CG47 anti tag coimmunoprecipitation 18313049 physical association X 2 Q68DV7 P0CG47 anti tag coimmunoprecipitation 18313049 physical association X 2 Q68DV7 Q15233 confocal microscopy 18655028 colocalization X 2 Q68DV7 P62837 two hybrid array 19690564 physical association X 2 Q68DV7 A0A024RDH2 two hybrid array 19690564 physical association X 2 Q68DV7 A0A024RA90 two hybrid array 19690564 physical association X 2 Q68DV7 A0A024R2K3 two hybrid array 19690564 physical association X 2 Q68DV7 P61088 two hybrid array 19690564 physical association X 2 Q969T4 Q68DV7 two hybrid array 19690564 physical association X 2 Q68DV7 pull down 18655028 physical association C 3 Q68DV7 anti tag coimmunoprecipitation 18655028 physical association C 3 Q68DV7 enzymatic study 18313049 ubiquitination reaction C 4 Q68DV7 enzymatic study 18313049 ubiquitination reaction C 4 Q9ULX6 Q68DV7 anti tag coimmunoprecipitation 18313049 physical association X 2 Q9ULX6 Q68DV7 two hybrid 18313049 physical association X 2 Q68DV7 P23246 confocal microscopy 18655028 colocalization X 2 Q68DV7 A0A024QZJ2 two hybrid array 19690564 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B UBE2D1 RNF43 MI:0018(two hybrid)|MI:0397(two hybrid array) 19690564 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_123432|UniProt_102421 bait|bait prey|prey RNF43 AKAP8L MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 18313049 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction) (intact)|(intact)|(biogrid)|(biogrid) UniProt_94753|UniProt_94754|BIOGRID_117308|BIOGRID_117307 bait|bait|bait|prey prey|prey|prey|bait UBE2E1 RNF43 MI:0397(two hybrid array)|MI:0018(two hybrid) 19690564 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction) (intact)|(biogrid) UniProt_102541|BIOGRID_123552 bait|bait prey|prey RNF43 UBE2D4 MI:0397(two hybrid array)|MI:0018(two hybrid) 19690564 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction) (intact)|(biogrid) UniProt_102517|BIOGRID_123528 prey|prey bait|bait RNF43 HECW1 MI:0018(two hybrid)|MI:0018(two hybrid) 21108931 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(biogrid) BIOGRID_200162|BIOGRID_200163 bait|bait prey|prey UBE2D2 RNF43 MI:0018(two hybrid)|MI:0397(two hybrid array)|MI:0018(two hybrid) 18313049,19690564 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0220(ubiquitination reaction)|MI:0915(physical association)|MI:0407(direct interaction) (intact)|(intact)|(biogrid) UniProt_94757|UniProt_102451|BIOGRID_123463 ancillary|bait|bait unspecified role|prey|prey RSPO1 RNF43 MI:0096(pull down) 23756651 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_281364 prey bait UBC RNF43 MI:0004(affinity chromatography technology) 18313049 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_117306 prey bait UBE2D3 RNF43 MI:0397(two hybrid array)|MI:0018(two hybrid)|MI:0018(two hybrid) 18313049,19690564 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0220(ubiquitination reaction)|MI:0407(direct interaction) (intact)|(intact)|(biogrid) UniProt_102484|UniProt_94758|BIOGRID_123496 bait|ancillary|bait prey|unspecified role|prey UBB RNF43 MI:0018(two hybrid)|MI:0018(two hybrid) 18313049 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(intact) UniProt_94755|UniProt_94756 prey|prey bait|bait NONO RNF43 MI:0096(pull down)|MI:0096(pull down)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0096(pull down) 18655028 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0403(colocalization) (intact)|(intact)|(biogrid)|(biogrid)|(intact) UniProt_96967|UniProt_96966|BIOGRID_255269|BIOGRID_255267|UniProt_96969 prey|prey|prey|prey|neutral component bait|bait|bait|bait|neutral component SFPQ RNF43 MI:0096(pull down)|MI:0096(pull down)|MI:0004(affinity chromatography technology)|MI:0096(pull down)|MI:0004(affinity chromatography technology) 18655028 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0403(colocalization)|MI:0915(physical association) (intact)|(intact)|(biogrid)|(intact)|(biogrid) UniProt_96967|UniProt_96966|BIOGRID_255268|UniProt_96968|BIOGRID_255266 prey|prey|prey|neutral component|prey bait|bait|bait|neutral component|bait UBE2N RNF43 MI:0018(two hybrid)|MI:0397(two hybrid array) 19690564 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_123625|UniProt_102614 bait|bait prey|prey p53 RNF43 MI:0018(two hybrid)|MI:0018(two hybrid) 21108931 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid) BIOGRID_200168|BIOGRID_200167 prey|bait bait|prey RNF43 UBE2E3 MI:0397(two hybrid array)|MI:0018(two hybrid) 19690564 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction) (intact)|(biogrid) UniProt_102560|BIOGRID_123570 prey|prey bait|bait RNF43 KDM5B MI:0004(affinity chromatography technology) 19336002 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_122821 prey bait

PPI - HINT Gene A Gene B PMID Method Quality DVL2 RNF43 25891077,25825523 0004,0004 LC RSPO1 RNF43 25825523,23756651 0004,0096 LC DVL2 RNF43 25891077,25825523 0004,0004 LC RSPO1 RNF43 25825523,23756651 0004,0096 LC UBE2D1 RNF43 19690564,19690564 0397,0018 HT UBE2E1 RNF43 19690564,19690564 0397,0018 HT UBE2D3 RNF43 19690564,18313049,18313049,19690564 0397,0096,0415,0018 HT,LC UBE2N RNF43 19690564,19690564 0397,0018 HT UBE2D2 RNF43 19690564,18313049,18313049,19690564 0397,0096,0415,0018 HT,LC RNF43 RNF43 18313049 0415 LC RNF43 HECW1 21108931,21108931 0004,0018 LC RNF43 UBE2E3 19690564,19690564 0397,0018 HT RNF43 AKAP8L 18313049,18313049,18313049 0004,0007,0018 LC RNF43 UBE2D4 19690564,19690564 0397,0018 HT RNF43 HEL-S-71 19690564 0018 HT

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q68DV7 O14641 RNF43 DVL2 0.507 25891077,25825523 Q68DV7 P23246 RNF43 SFPQ 0.569 18655028 Q68DV7 P61088 RNF43 UBE2N 0.286 19690564 Q68DV7 P0CG47 RNF43 UBB 0.376 18313049 Q68DV7 Q29504 RNF43 UBA1 0.033 18313049 Q68DV7 P04637 RNF43 TP53 0.376 21108931 Q68DV7 P51965 RNF43 UBE2E1 0.376 19690564 Q9ULX6 Q68DV7 AKAP8L RNF43 0.702 18313049 Q9NQB0 Q68DV7 TCF7L2 RNF43 0.309 26350900 Q9Y2X8 Q68DV7 UBE2D4 RNF43 0.286 19690564 Q969T4 Q68DV7 UBE2E3 RNF43 0.286 19690564 Q68DV7 P61077 RNF43 UBE2D3 0.523 19690564,18313049 Q68DV7 Q68DV7 RNF43 RNF43 0.376 18313049 Q76N89 Q68DV7 HECW1 RNF43 0.454 21108931 Q9UGL1 Q68DV7 KDM5B RNF43 0.21 19336002 Q68DV7 P62837 RNF43 UBE2D2 0.523 19690564,18313049 Q68DV7 Q15233 RNF43 NONO 0.623 18655028 Q68DV7 Q13467 RNF43 FZD5 0.376 25825523 Q68DV7 P51668 RNF43 UBE2D1 0.286 19690564 Q68DV7 Q2MKA7 RNF43 RSPO1 0.454 23756651,25825523

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 DVL2 RNF43 MI:0045 experimental interaction detection MI:0461 interaction database LGR5 RNF43 MI:0362 inference MI:1106 pathways database LRP6 RNF43 MI:0362 inference MI:1106 pathways database TP53 RNF43 MI:0045 experimental interaction detection MI:0461 interaction database UBB RNF43 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database UBC RNF43 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database UBA1 RNF43 MI:0362 inference MI:0461 interaction database SFPQ RNF43 MI:0045 experimental interaction detection MI:0461 interaction database UBE2D1 RNF43 MI:0045 experimental interaction detection MI:0461 interaction database UBE2E1 RNF43 MI:0045 experimental interaction detection MI:0461 interaction database RAD23B RNF43 MI:0362 inference MI:0461 interaction database WNT3A RNF43 MI:0362 inference MI:1106 pathways database UBE2D3 RNF43 MI:0045 experimental interaction detection MI:0461 interaction database UBE2N RNF43 MI:0045 experimental interaction detection MI:0461 interaction database UBE2D2 RNF43 MI:0045 experimental interaction detection MI:0461 interaction database RPS27A RNF43 MI:0362 inference MI:1106 pathways database UBA52 RNF43 MI:0362 inference MI:1106 pathways database FZD5 RNF43 MI:0045 experimental interaction detection MI:0461 interaction database NONO RNF43 MI:0045 experimental interaction detection MI:0461 interaction database RSPO4 RNF43 MI:0362 inference MI:1106 pathways database RSPO1 RNF43 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database RNF43 RSPO2 MI:0362 inference MI:1106 pathways database RNF43 HECW1 MI:0045 experimental interaction detection MI:0461 interaction database RNF43 UBE2E3 MI:0045 experimental interaction detection MI:0461 interaction database RNF43 LGR4 MI:0362 inference MI:1106 pathways database RNF43 RSPO3 MI:0362 inference MI:1106 pathways database RNF43 FZD8 MI:0362 inference MI:0461 interaction database RNF43 LGR6 MI:0362 inference MI:1106 pathways database RNF43 TCF7L2 MI:0045 experimental interaction detection MI:0461 interaction database RNF43 KDM5B MI:0045 experimental interaction detection MI:0461 interaction database RNF43 ZNRF3 MI:0362 inference MI:1106 pathways database RNF43 FZD4 MI:0362 inference MI:1106 pathways database RNF43 AKAP8L MI:0045 experimental interaction detection MI:0461 interaction database RNF43 UBE2D4 MI:0045 experimental interaction detection MI:0461 interaction database