PTM - CPLM Gene Name UniProt ID Type Position Code SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 362 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 29 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 47 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 62 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 82 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 370 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 377 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 447 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 465 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 475 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 507 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 578 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 615 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 97 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 481 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 515 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 527 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 489 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 390 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 412 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 519 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 440 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 656 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 610 K SPG20; KIAA0610; TAHCCP1 Q8N0X7 Ubiquitination 526 K

PTM - dbPAF Gene Name UniProt ID Position Type Species SPG20; KIAA0610; TAHCCP1 Q8N0X7 101 T Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 102 S Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 114 Y Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 357 S Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 358 S Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 392 T Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 393 S Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 394 S Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 400 S Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 45 Y Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 46 Y Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 470 S Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 474 T Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 524 S Homo sapiens(Human) SPG20; KIAA0610; TAHCCP1 Q8N0X7 628 Y Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q8N0X7 K489-ac Acetylation QATGGAAkVSQFLVD Q8N0X7 R334-m1 Methylation LRQMSDLrLQANWNR Q8N0X7 K362-m1 Methylation RPssDQLkEASGTDV Q8N0X7 K370-m1 Methylation EASGTDVkQLDQGNk Q8N0X7 K382-m1 Methylation GNkDVRHkGKRGKRA Q8N0X7 Y45-p Phosphorylation QKEEAKNyykQGIGH Q8N0X7 Y46-p Phosphorylation KEEAKNyykQGIGHL Q8N0X7 S58-p Phosphorylation GHLLRGIsISSkESE Q8N0X7 T101-p Phosphorylation ILEKGLAtsLQNDLQ Q8N0X7 S102-p Phosphorylation LEKGLAtsLQNDLQE Q8N0X7 Y114-p Phosphorylation LQEVPKLyPEFPPKD Q8N0X7 S357-p Phosphorylation IPGRTRPssDQLkEA Q8N0X7 S358-p Phosphorylation PGRTRPssDQLkEAS Q8N0X7 S393-p Phosphorylation GKRAkDTsSEEVNLs Q8N0X7 S400-p Phosphorylation sSEEVNLsHIVPCEP Q8N0X7 S470-p Phosphorylation EEkPVEVsPAVTkGL Q8N0X7 S524-p Phosphorylation GSkLVPEsLKKDkDG Q8N0X7 Y628-p Phosphorylation GHTLLEDyQIVDNSQ Q8N0X7 K29-ub Ubiquitination KAFLFVNkGLNTDEL Q8N0X7 K47-ub Ubiquitination EEAKNyykQGIGHLL Q8N0X7 K62-ub Ubiquitination RGIsISSkESEHTGP Q8N0X7 K82-ub Ubiquitination RQMQQKMkETLQNVR Q8N0X7 K287-ub Ubiquitination PDRSPVLkCTAGAYM Q8N0X7 K362-ub Ubiquitination RPssDQLkEASGTDV Q8N0X7 K370-ub Ubiquitination EASGTDVkQLDQGNk Q8N0X7 K377-ub Ubiquitination kQLDQGNkDVRHkGK Q8N0X7 K390-ub Ubiquitination GKRGKRAkDTsSEEV Q8N0X7 K422-ub Ubiquitination ELPEWSEkVAHNILS Q8N0X7 K440-ub Ubiquitination WVSWGLVkGAEITGk Q8N0X7 K447-ub Ubiquitination kGAEITGkAIQKGAS Q8N0X7 K465-ub Ubiquitination ERIQPEEkPVEVsPA Q8N0X7 K475-ub Ubiquitination EVsPAVTkGLYIAkQ Q8N0X7 K481-ub Ubiquitination TkGLYIAkQATGGAA Q8N0X7 K489-ub Ubiquitination QATGGAAkVSQFLVD Q8N0X7 K507-ub Ubiquitination TVANCVGkELAPHVk Q8N0X7 K514-ub Ubiquitination kELAPHVkKHGSkLV Q8N0X7 K519-ub Ubiquitination HVkKHGSkLVPEsLK Q8N0X7 K529-ub Ubiquitination PEsLKKDkDGkSPLD Q8N0X7 K532-ub Ubiquitination LKKDkDGkSPLDGAM Q8N0X7 K578-ub Ubiquitination TVQTVRYkYGYNAGE Q8N0X7 K615-ub Ubiquitination GIKAMVKkTATQTGH

PTM - dbPTM UniProt ID Position Code Type Resources References Q8N0X7 1 M Acetylation Swiss-Prot 1010711 19413330 Q8N0X7 362 K Ubiquitylation Swiss-Prot 1010711 18781797 Q8N0X7 470 S Phosphorylation Swiss-Prot 1010711 18669648 Q8N0X7 470 S Phosphorylation PhosphoELM.10011 18669648 Q8N0X7 29 K Ubiquitylation Phosphositeplus.1010730 21890473 Q8N0X7 362 K Ubiquitylation Phosphositeplus.1010730 18781797;20972266;21890473;21906983 Q8N0X7 370 K Ubiquitylation Phosphositeplus.1010730 21890473 Q8N0X7 377 K Ubiquitylation Phosphositeplus.1010730 21890473 Q8N0X7 440 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8N0X7 465 K Ubiquitylation Phosphositeplus.1010730 21890473 Q8N0X7 47 K Ubiquitylation Phosphositeplus.1010730 21890473 Q8N0X7 475 K Ubiquitylation Phosphositeplus.1010730 21890473 Q8N0X7 578 K Ubiquitylation Phosphositeplus.1010730 21890473 Q8N0X7 62 K Ubiquitylation Phosphositeplus.1010730 21890473 Q8N0X7 82 K Ubiquitylation Phosphositeplus.1010730 21890473 Q8N0X7 357 S Phosphorylation Phosphositeplus.1010730 20736484 Q8N0X7 470 S Phosphorylation Phosphositeplus.1010730 18669648;20068231 Q8N0X7 46 Y Phosphorylation Phosphositeplus.1010730 21049007 Q8N0X7 628 Y Phosphorylation Phosphositeplus.1010730 20007894;20562096 Q8N0X7 470 S Phosphorylation HPRD 9.0 18669648,20068231 Q8N0X7 499 C S-nitrosylation dbSNO 1.0 19483679 Q8N0X7 504 C S-nitrosylation dbSNO 1.0 19483679 Q8N0X7 362 K Ubiquitylation SysPTM 1.1 18781797 Q8N0X7 470 S Phosphorylation SysPTM 1.1 18669648

PTM - HPRD Symbol Position Code Type References SPG20 470 S Phosphorylation 18669648,20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q8N0X7 470 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q8N0X7 1 N-acetylmethionine 19413330;22814378 Q8N0X7 470 Phosphoserine 18669648;20068231;23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type SPG20 IPI00430622 S 470 EKPVEVpSPAVTKG phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 23111 116734 - K Ubiquitination 19580544 23111 116734 - K Ubiquitination 20504295 23111 116734 - K Ubiquitination 22118674 23111 116734 - K Ubiquitination 18781797 23111 116734 - K Ubiquitination 21987572 23111 116734 - K Ubiquitination 20972266 23111 116734 - K Ubiquitination 21139048 23111 116734 - K Ubiquitination 21890473 23111 116734 - K Ubiquitination 21906983 23111 116734 - K Sumoylation 19471022 23111 116734 - K Ubiquitination 19307600 23111 116734 - K Ubiquitination 22053931 23111 116734 - K Ubiquitination 22505724 23111 116734 - K Ubiquitination 21963094 23111 116734 - K Ubiquitination 23000965 23111 116734 - K Ubiquitination 24816145 23111 116734 - K Ubiquitination 25015289 23111 116734 - K Ubiquitination 25147182

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID SPG20 Homo sapiens (Human) 578; 615; 97; 370; 362 "578,VQTVRYKYGYNAG; 615,IKAMVKKTATQTG; 97,RLEILEKGLATSL; 370,ASGTDVKQLDQGN; 362,PSSDQLKEASGTD" 578:22053931; 615:22053931; 97:22053931; 370:22053931; 362:22053931 SPG20 Homo sapiens (Human) 578; 475; 82; 370; 447; 507; 29; 377; 47; 62; 362; 465 "578,VQTVRYKYGYNAG; 475,VSPAVTKGLYIAK; 82,QMQQKMKETLQNV; 370,ASGTDVKQLDQGN; 447,GAEITGKAIQKGA; 507,VANCVGKELAPHV; 29,AFLFVNKGLNTDE; 377,QLDQGNKDVRHKG; 47,EAKNYYKQGIGHL; 62,GISISSKESEHTG; 362,PSSDQLKEASGTD; 465,RIQPEEKPVEVSP" 578:21890473; 475:21890473; 82:21890473; 370:21890473; 447:21890473; 507:21890473; 29:21890473; 377:21890473; 47:21890473; 62:21890473; 362:21890473; 465:21890473 SPG20 Homo sapiens (Human) 578; 475; 82; 370; 447; 507; 29; 377; 47; 62; 362; 465 "578,VQTVRYKYGYNAG; 475,VSPAVTKGLYIAK; 82,QMQQKMKETLQNV; 370,ASGTDVKQLDQGN; 447,GAEITGKAIQKGA; 507,VANCVGKELAPHV; 29,AFLFVNKGLNTDE; 377,QLDQGNKDVRHKG; 47,EAKNYYKQGIGHL; 62,GISISSKESEHTG; 362,PSSDQLKEASGTD; 465,RIQPEEKPVEVSP" 578:21890473; 475:21890473; 82:21890473; 370:21890473; 447:21890473; 507:21890473; 29:21890473; 377:21890473; 47:21890473; 62:21890473; 362:21890473; 465:21890473 SPG20 Homo sapiens (Human) 578; 475; 82; 370; 447; 507; 29; 377; 47; 62; 362; 465 "578,VQTVRYKYGYNAG; 475,VSPAVTKGLYIAK; 82,QMQQKMKETLQNV; 370,ASGTDVKQLDQGN; 447,GAEITGKAIQKGA; 507,VANCVGKELAPHV; 29,AFLFVNKGLNTDE; 377,QLDQGNKDVRHKG; 47,EAKNYYKQGIGHL; 62,GISISSKESEHTG; 362,PSSDQLKEASGTD; 465,RIQPEEKPVEVSP" 578:21890473; 475:21890473; 82:21890473; 370:21890473; 447:21890473; 507:21890473; 29:21890473; 377:21890473; 47:21890473; 62:21890473; 362:21890473; 465:21890473 SPG20 Homo sapiens (Human) 578; 475; 82; 370; 447; 507; 29; 377; 47; 62; 362; 465 "578,VQTVRYKYGYNAG; 475,VSPAVTKGLYIAK; 82,QMQQKMKETLQNV; 370,ASGTDVKQLDQGN; 447,GAEITGKAIQKGA; 507,VANCVGKELAPHV; 29,AFLFVNKGLNTDE; 377,QLDQGNKDVRHKG; 47,EAKNYYKQGIGHL; 62,GISISSKESEHTG; 362,PSSDQLKEASGTD; 465,RIQPEEKPVEVSP" 578:21890473; 475:21890473; 82:21890473; 370:21890473; 447:21890473; 507:21890473; 29:21890473; 377:21890473; 47:21890473; 62:21890473; 362:21890473; 465:21890473