PTM - CPLM Gene Name UniProt ID Type Position Code UBA3; UBE1C Q8TBC4 Methylation 9 K UBA3; UBE1C Q8TBC4 Methylation 10 K UBA3; UBE1C Q8TBC4 Ubiquitination 147 K UBA3; UBE1C Q8TBC4 Ubiquitination 284 K UBA3; UBE1C Q8TBC4 Ubiquitination 307 K UBA3; UBE1C Q8TBC4 Ubiquitination 361 K UBA3; UBE1C Q8TBC4 Ubiquitination 124 K UBA3; UBE1C Q8TBC4 Ubiquitination 21 K UBA3; UBE1C Q8TBC4 Ubiquitination 409 K UBA3; UBE1C Q8TBC4 Ubiquitination 434 K UBA3; UBE1C Q8TBC4 Ubiquitination 431 K UBA3; UBE1C Q8TBC4 Ubiquitination 86 K UBA3; UBE1C Q8TBC4 Ubiquitination 260 K UBA3; UBE1C Q8TBC4 Ubiquitination 398 K

PTM - dbPAF Gene Name UniProt ID Position Type Species UBA3; UBE1C Q8TBC4 103 T Homo sapiens(Human) UBA3; UBE1C Q8TBC4 107 S Homo sapiens(Human) UBA3; UBE1C Q8TBC4 185 Y Homo sapiens(Human) UBA3; UBE1C Q8TBC4 203 T Homo sapiens(Human) UBA3; UBE1C Q8TBC4 377 S Homo sapiens(Human) UBA3; UBE1C Q8TBC4 390 T Homo sapiens(Human) UBA3; UBE1C Q8TBC4 392 S Homo sapiens(Human) UBA3; UBE1C Q8TBC4 394 S Homo sapiens(Human) UBA3; UBE1C Q8TBC4 399 S Homo sapiens(Human) UBA3; UBE1C Q8TBC4 91 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q8TBC4 K409-ac Acetylation ITATLEGkNRTLYLQ Q8TBC4 K9-m1 Methylation ADGEEPEkkRRRIEE Q8TBC4 K10-m1 Methylation DGEEPEkkRRRIEEL Q8TBC4 R135-m1 Methylation AAEFLNDrVPNCNVV Q8TBC4 R295-m1 Methylation RASQYNIrGVTYRLT Q8TBC4 S91-p Phosphorylation LLkNLALsGFRQIHV Q8TBC4 T103-p Phosphorylation IHVIDMDtIDVsNLN Q8TBC4 S107-p Phosphorylation DMDtIDVsNLNRQFL Q8TBC4 Y185-p Phosphorylation MLISLLNyEDGVLDP Q8TBC4 T203-p Phosphorylation VPLIDGGtEGFkGNA Q8TBC4 S377-p Phosphorylation LPQNIQFsPSAKLQE Q8TBC4 T390-p Phosphorylation QEVLDYLtNsAsLQM Q8TBC4 S392-p Phosphorylation VLDYLtNsAsLQMks Q8TBC4 S394-p Phosphorylation DYLtNsAsLQMksPA Q8TBC4 S399-p Phosphorylation sAsLQMksPAITATL Q8TBC4 K86-ub Ubiquitination GLGCELLkNLALsGF Q8TBC4 K147-ub Ubiquitination NVVPHFNkIQDFNDT Q8TBC4 K207-ub Ubiquitination DGGtEGFkGNARVIL Q8TBC4 K260-ub Ubiquitination VRMLQWPkEQPFGEG Q8TBC4 K284-ub Ubiquitination HIQWIFQkSLERASQ Q8TBC4 K307-ub Ubiquitination RLTQGVVkRIIPAVA Q8TBC4 K361-ub Ubiquitination YTFEAERkENCPACS Q8TBC4 K398-ub Ubiquitination NsAsLQMksPAITAT Q8TBC4 K409-ub Ubiquitination ITATLEGkNRTLYLQ Q8TBC4 K434-ub Ubiquitination PNLSKTLkELGLVDG

PTM - dbPTM UniProt ID Position Code Type Resources References Q8TBC4 147 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q8TBC4 260 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8TBC4 284 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q8TBC4 307 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q8TBC4 361 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q8TBC4 398 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8TBC4 409 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8TBC4 434 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8TBC4 86 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8TBC4 107 S Phosphorylation Phosphositeplus.1010730 21406692 Q8TBC4 399 S Phosphorylation Phosphositeplus.1010730 20068231 Q8TBC4 91 S Phosphorylation Phosphositeplus.1010730 20068231 Q8TBC4 103 T Phosphorylation Phosphositeplus.1010730 21406692 Q8TBC4 91 S Phosphorylation HPRD 9.0 20068231 Q8TBC4 399 S Phosphorylation HPRD 9.0 20068231 Q8TBC4 390 T Phosphorylation HPRD 9.0 18212344 Q8TBC4 390 T Phosphorylation SysPTM 1.1 18212344

PTM - HPRD Symbol Position Code Type References UBA3 290 T Phosphorylation 18212344 UBA3 299 S Phosphorylation 20068231 UBA3 376 T Phosphorylation 18212344 UBA3 385 S Phosphorylation 20068231 UBA3 390 T Phosphorylation 18212344 UBA3 399 S Phosphorylation 20068231 UBA3 77 S Phosphorylation 20068231 UBA3 91 S Phosphorylation 20068231

PTM - UniProt UniProt ID Position Type PMIDs Q8TBC4 2 N-acetylalanine 22814378

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 9039 114503 - K Ubiquitination 21963094 9039 114503 - K Ubiquitination 21139048 9039 114503 - K Ubiquitination 21890473 9039 114503 - K Ubiquitination 21906983 9039 114503 - K Ubiquitination 22505724 9039 114503 - K Ubiquitination 23000965 9039 114503 - K Neddylation 24654937 9039 114503 - K Ubiquitination 24816145 9039 114503 - K Ubiquitination 25015289 9039 114503 - K Ubiquitination 25147182 9039 114503 - K Neddylation 18247557

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID UBA3 Homo sapiens (Human) 307; 86; 409; 398; 260; 434; 147; 361; 284 "307,LTQGVVKRIIPAV; 86,LGCELLKNLALSG; 409,TATLEGKNRTLYL; 398,SASLQMKSPAITA; 260,RMLQWPKEQPFGE; 434,NLSKTLKELGLVD; 147,VVPHFNKIQDFND; 361,TFEAERKENCPAC; 284,IQWIFQKSLERAS" "307:21890473,21906983; 86:21906983; 409:21906983; 398:21906983; 260:21906983; 434:21906983; 147:21890473,21906983; 361:21890473,21906983; 284:21890473,21906983" UBA3 Homo sapiens (Human) 293; 133; 270; 347 "293,LTQGVVKRIIPAV; 133,VVPHFNKIQDFND; 270,IQWIFQKSLERAS; 347,TFEAERKENCPAC" 293:21890473; 133:21890473; 270:21890473; 347:21890473 UBA3 Homo sapiens (Human) 293; 133; 270; 347 "293,LTQGVVKRIIPAV; 133,VVPHFNKIQDFND; 270,IQWIFQKSLERAS; 347,TFEAERKENCPAC" 293:21890473; 133:21890473; 270:21890473; 347:21890473 UBA3 Homo sapiens (Human) 320; 266; 106; 243 "320,TFEAERKENCPAC; 266,LTQGVVKRIIPAV; 106,VVPHFNKIQDFND; 243,IQWIFQKSLERAS" 307:21906983; 86:21906983; 409:21906983; 398:21906983; 260:21906983; 434:21906983; 147:21906983; 361:21906983; 284:21906983 UBA3 Homo sapiens (Human) 307; 260; 147; 361; 284 "307,LTQGVVKRIIPAV; 260,RMLQWPKEQPFGE; 147,VVPHFNKIQDFND; 361,TFEAERKENCPAC; 284,IQWIFQKSLERAS" "307:21890473; 260:21906983; 147:21906983,21890473; 361:21890473; 284:21906983,21890473" UBA3 Homo sapiens (Human) 307; 147; 361; 284 "307,LTQGVVKRIIPAV; 147,VVPHFNKIQDFND; 361,TFEAERKENCPAC; 284,IQWIFQKSLERAS" 307:21890473; 147:21890473; 361:21890473; 284:21890473 UBA3 Homo sapiens (Human) 106; 243 "106,VVPHFNKIQDFND; 243,IQWIFQKSLERAS" 106:21890473; 243:21890473