PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus Btk Tuba1a 15102471 intact exp G G G G G Btk Luc7l3 15102471 mint,intact exp G G G G G Btk Ticam1 21441935 innatedb,intact exp Btk Inpp5d 16601135 biogrid exp G G G G G Btk Ighmbp2 15102471 mint,intact exp Pik3r1 Btk 18652890,17020802 innatedb exp G G G G G Btk Btk-PH-domain binding protein, Myeloid/lymphoid or mixed-lineage leukemia (Trithorax homolog, Drosophila) translocated to, 1, Protein Mllt1 11007757 innatedb,biogrid exp G G G Irf2 Btk 18652890 innatedb exp G G G G G Ftl1 Btk 15102471 intact exp Btk Actc1 15102471 intact exp G Btk Cbx1 15102471 mint,intact exp G G G G G H2afz Btk 15102471 intact exp G G G G G Smarce1 Btk 15102471 mint,intact exp G G G G G Btk Fthl17 mint exp Btk Pin1 16644721 innatedb exp Btk Dok3 24929003 innatedb exp G G G G G Btk Ddx5 15102471 mint,intact exp G G G G G Lox Btk 15102471 mint,intact exp G G G G Btk Ppp3cb 15102471 mint,intact exp G G G G G Btk Mrc1 15102471 mint,intact exp G G G G G Btk Ap3b1 15102471 mint,intact exp G G G G G Btk Kcmf1 15102471 mint,intact exp G G G G G Aldoa Btk 15102471 mint,intact exp G G G G G Man2b1 Btk 15102471 mint,intact exp G G G G G Rpl7 Btk 15102471 mint,intact exp G G G G G Btk Ppm1g 15102471 mint,intact exp G G G G G Cd40 Btk 21441935 innatedb,intact exp G G G G G Btk MCG2065, isoform CRA_c, PreS1 binding protein, Protein Gltscr2 15102471 mint,intact exp G G G G G Glul Btk 15102471 mint,intact exp G G G G G Btk Pfkp 15102471 mint,intact exp G G G G G Btk Arid3a 15456761 biogrid exp, ortho G G G G G Btk Acta1 mint exp, ortho G G G G G Btk Stat3 17367410 innatedb exp, ortho G G G G G Btk Ikzf1 23977012 innatedb exp, ortho G G G G Btk Btk 11007757,23977012,15102471 innatedb,intact exp, ortho G G G G G Btk Eef1a2 15102471 mint,intact exp, ortho G G G G G Btk Plcg2 19150402 innatedb exp, ortho G G G G G Cd19 Btk 12023340 innatedb exp, ortho G G G G Btk Gnb1 7972043 biogrid exp, ortho G G G G G Btk Blnk 15087455,18329366 biogrid,intact exp, ortho G G G G G Btk Grb2 15102471 mint,intact exp, ortho G G G G G Btk Gng2 7972043 biogrid exp, ortho G G G G G Btk Cmtm3 15087455 innatedb exp, ortho G G G G G Btk Was 22253930,15102471 innatedb,mint,intact exp, ortho G G G G G Btk Tirap 12724322 innatedb exp, ortho G G G G G Btk Tlr2 17020802 innatedb exp, ortho G G G G G Myd88 Btk 21441935 innatedb,intact exp, ortho G G G G G Btk Mylk2 ortho Gna12 Btk ortho G G G G G Btk Mapk8 ortho G G G G G Btk Cav1 ortho G G G G G Btk Tlr4 ortho G G G G G Btk Bmx ortho G G G Btk Pik3cg ortho G G G G Hsp90aa1 Btk ortho G G G G G Map3k5 Btk ortho G G G G G Btk Jak1 ortho G G G G G Pip4k2a Btk ortho G G G G G Btk Prkd1 ortho G G Btk Ndufs1 ortho G G G G G Btk Cblb ortho Btk Pik3r3 ortho Btk Irak1 ortho G G G G G Btk Plcg1 ortho Btk Fgfr4 ortho G Btk Ptk2b ortho G G G G G Btk Camkk2 ortho G G G G G Btk Krt5 ortho Btk Wasf2 ortho G G G G G Btk Tnfrsf10b ortho Tec Btk ortho G G G G G Btk Atp5b ortho G G G G G Btk Mpp7 ortho G G G G Btk Gab1 ortho G G G G G Pkd1 Btk ortho G G G G Btk Pip5k1a ortho G G G G G Btk Prkcz ortho G G Btk Atp5a1 ortho G G G G G Becn1 Btk ortho G G G G G Blk Btk ortho G G G G Btk Sfpq ortho G G G G G Lyn Btk ortho Btk Coro1b ortho G G G G G Btk Khdrbs1 ortho G G G G G Btk Tlr9 ortho Btk Stat5a ortho G G G G G Btk Tuba4a ortho G G G G G Met Btk ortho G G G G G Vav1 Btk ortho G G G G Btk Flna ortho G G G G G Btk Bcr ortho G G G G G Btk Ibtk ortho G G G G G Btk Tubb4b ortho G G G G G Fcer1g Btk ortho G G G G G App Btk ortho G G G G G Btk Hadha ortho G G G G G Btk Pip5k1b ortho G G G G Btk Tlr8 ortho G G G Pip5k1c Btk ortho G G G G G Btk Rps6kb1 ortho G G G G G Spta1 Btk ortho G G G Prkce Btk ortho G G G G G Cflar Btk ortho G G G G G Btk Twf1 ortho Hsp90ab1 Btk ortho G G G G G Kit Btk ortho G G G G G Lat Btk ortho G G G G G Btk Itk ortho G G Btk Tlr6 ortho G G G Btk Fyn ortho G G G G G Btk Matk ortho G G G Plau Btk ortho Btk Bcl2l1 ortho G G G G G Abl1 Btk ortho G G G G G Btk Ddx3x ortho G G G G G Btk Bax ortho G G G G G Btk Med28 ortho G G G G G Btk Pip4k2c ortho G G G G G Tyr Btk ortho Btk Sh3bp5 ortho G G G G G Btk Ccne1 ortho G G G Btk Coro1c ortho G G G G G Btk Pikfyve ortho G G Bcl2l11 Btk ortho G G G G G Btk Gtf2i ortho G G G G G Btk Sh2b2 ortho G Btk Rps6kb2 ortho G G G G G Btk Nck1 ortho G G G G G Protein Tnfrsf25, Tumor necrosis factor receptor superfamily, member 25, isoform CRA_b Btk ortho G G Btk Arrb1 ortho G G G G G Pdcd6 Btk ortho G G G G G Btk Prkcb ortho G G G G G Btk Afg3l2 ortho G G G G G Cbl Btk ortho G G G G G Btk Daam1 ortho Btk Csk ortho G G G G G Btk Faslg ortho G Btk Lcp2 ortho G G G G G Ar Btk ortho Btk Dbn1 ortho G G G G Tp53 Btk ortho G G G G G Hck Btk ortho G G G G G Btk Jak2 ortho G G G G G Gp6 Btk ortho Btk Rela ortho G G G G G Btk Prkcq ortho G G G G G Btk Arhgap17 ortho G G G G G Btk Rftn1 ortho Btk Dapp1 ortho G G G G G Btk Pip4k2b ortho Prkca Btk ortho G G G G G Btk Syk ortho G G G G G Gnaq Btk ortho G G G Btk Pik3ap1 ortho G G Btk Dapk1 ortho G G G G G Ccnd2 Btk ortho G G G G G Fas Btk ortho G G G G G Btk Hspg2 ortho G G Btk Actn1 ortho G G G G G Btk Rps3 ortho G G G G G Btk Mrps22 ortho G G G G G Mal Btk ortho G Btk Jak3 ortho G G G G G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants P35991 Q9QUN3 affinity chromatography technology 15087455 physical association X 2 P35991 affinity chromatography technology 11007757 physical association X 2 P35991 Q9ES52-4 affinity chromatography technology 16601135 physical association X 2 P35991 P62874 affinity chromatography technology 7972043 physical association X 2 P63213 P35991 affinity chromatography technology 7972043 physical association X 2 Q62431 P35991 affinity chromatography technology 15456761 physical association X 2 P35991 coimmunoprecipitation 15087455 association C 4 P35991 far western blotting 7972043 association C 3 Q61830 P35991 two hybrid 15102471 physical association X 2 P35991 Q61656 two hybrid 15102471 physical association X 2 P35991 P05064 two hybrid 15102471 physical association X 2 P35991 P15105 two hybrid 15102471 physical association X 2 P35991 Q80UY2 two hybrid 15102471 physical association X 2 P35991 O09159 two hybrid 15102471 physical association X 2 P35991 P48453 two hybrid 15102471 physical association X 2 P35991 Q8BTX4 two hybrid 15102471 physical association X 2 P35991 P83917 two hybrid 15102471 physical association X 2 P35991 Q9QUN3 fluorescence microscopy 18329366 colocalization X 2 P35991 Q9QUN3 anti bait coimmunoprecipitation 18329366 physical association X 2 P68369 P35991 two hybrid 15102471 physical association X 2 Q61074 P35991 two hybrid 15102471 physical association X 2 P14148 P35991 two hybrid 15102471 physical association X 2 P0C0S6 P35991 two hybrid 15102471 physical association X 2 P62631 P35991 two hybrid 15102471 physical association X 2 Q80UF7 P35991 anti tag coimmunoprecipitation 21441935 physical association X 2 Q9WUA3 P35991 two hybrid 15102471 physical association X 2 Q60631 P35991 two hybrid 15102471 physical association X 2 Q60631 P35991 two hybrid 15102471 physical association X 2 Q60631 P35991 two hybrid 15102471 physical association X 2 Q60631 P35991 two hybrid 15102471 physical association X 2 P78347 P35991 coimmunoprecipitation 9012831 physical association X 2 P35991 anti bait coimmunoprecipitation 21441935 association C 3 P29391 P35991 two hybrid 15102471 physical association X 2 P35991 O54941 two hybrid 15102471 physical association X 2 P35991 Q9Z1T1 two hybrid 15102471 physical association X 2 P35991 P68033 two hybrid 15102471 physical association X 2 P35991 P28301 two hybrid 15102471 physical association X 2 P35991 P40694 two hybrid 15102471 physical association X 2 P35991 Q5SUF2 two hybrid 15102471 physical association X 2 P35991 P27512 confocal microscopy 21441935 colocalization X 2 P35991 P27512 anti tag coimmunoprecipitation 21441935 physical association X 2 P35991 P27512 anti bait coimmunoprecipitation 21441935 physical association X 2 P22366 P35991 anti tag coimmunoprecipitation 21441935 physical association X 2 P70315 P35991 two hybrid 15102471 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B Btk Arid3a MI:0004(affinity chromatography technology) 15456761 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_328663 prey bait Btk Was MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_164783 prey bait Btk Gnb1 MI:0004(affinity chromatography technology) 7972043 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_328143 bait prey Btk Ppm1g MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162769 bait prey Btk Ap3b1 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_164880 bait prey Cd40 Btk MI:0006(anti bait coimmunoprecipitation)|MI:0006(anti bait coimmunoprecipitation)|MI:0006(anti bait coimmunoprecipitation) 21441935 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association)|MI:0403(colocalization) (intact)|(intact)|(intact) UniProt_171912|UniProt_171908|UniProt_171909 prey|prey|neutral component bait|bait|neutral component Ftl1 Btk MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162804 prey bait Man2b1 Btk MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162743 prey bait Smarce1 Btk MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162761 prey bait Btk Luc7l3 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_163020 bait prey Btk Ddx5 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_164881 bait prey Btk Actc1 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_164953 bait prey Btk Inpp5d MI:0004(affinity chromatography technology) 16601135 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_322039 prey bait Btk Eef1a2 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162728 bait prey Btk Gltscr2 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_163019 bait prey Lox Btk MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162829 prey bait Btk Mllt1 MI:0004(affinity chromatography technology) 11007757 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_325516 bait prey Btk Tuba1a MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162826 bait prey Btk Ighmbp2 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162737 bait prey Btk Gng2 MI:0004(affinity chromatography technology) 7972043 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (biogrid) BIOGRID_328145 bait prey H2afz Btk MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162807 prey bait Btk Blnk MI:0004(affinity chromatography technology)|MI:0416(fluorescence microscopy)|MI:0416(fluorescence microscopy) 15087455,18329366 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association)|MI:0403(colocalization) (biogrid)|(intact)|(intact) BIOGRID_319517|UniProt_168895|UniProt_168894 prey|prey|neutral component bait|bait|neutral component Myd88 Btk MI:0006(anti bait coimmunoprecipitation)|MI:0006(anti bait coimmunoprecipitation) 21441935 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0914(association) (intact)|(intact) UniProt_171910|UniProt_171907 prey|prey bait|bait Btk Cbx1 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_163021 bait prey Glul Btk MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_164830 prey bait Btk Kcmf1 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162820 bait prey Btk Pfkp MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162720 bait prey Btk Ppp3cb MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162747 bait null Btk Mrc1 MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162742 bait prey Aldoa Btk MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_164954 prey bait Btk Grb2 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association) (intact)|(intact)|(intact)|(intact) UniProt_164792|UniProt_164793|UniProt_164794|UniProt_164984 prey|prey|prey|prey bait|bait|bait|bait Rpl7 Btk MI:0018(two hybrid) 15102471 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association) (intact) UniProt_162754 prey bait Btk Ticam1 MI:0006(anti bait coimmunoprecipitation)|MI:0006(anti bait coimmunoprecipitation) 21441935 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0914(association)|MI:0915(physical association) (intact)|(intact) UniProt_171907|UniProt_171911 bait|bait prey|prey

PPI - HINT Gene A Gene B PMID Method Quality MAN2B1 BTK 15102471 0018 LC SMARCE1 BTK 15102471 0018 LC ALDOA BTK 15102471 0018 LC H2AFZ BTK 15102471 0018 LC RPL7 BTK 15102471 0018 LC GLUL BTK 15102471 0018 LC PIK3R1 BTK 17020802,17020802 0007,0059 LC LOX BTK 15102471 0018 LC FTL1 BTK 15102471 0018 LC BTK BTK 11007757 0424 LC BTK IGHMBP2 15102471 0018 LC BTK PPP3CB 15102471 0018 LC BTK EEF1A2 15102471 0018 LC BTK ACTC1 15102471 0018 LC BTK TUBA1A 15102471 0018 LC BTK WAS 15102471 0018 LC BTK CBX1 15102471 0018 LC BTK LUC7L3 15102471 0018 LC BTK GRB2 15102471 0018 LC BTK PPM1G 15102471 0018 LC BTK DDX5 15102471 0018 LC BTK MRC1 15102471 0018 LC BTK KCMF1 15102471 0018 LC BTK GLTSCR2 15102471 0018 LC BTK BLNK 18329366,18329366 0006,0416 LC BTK PFKP 15102471 0018 LC BTK AP3B1 15102471 0018 LC MYD88 BTK 21441935,21441935 0006,0007 LC IRF2 BTK 18652890 0007 LC CD19 BTK 12023340 0007 LC PIK3R1 BTK 17020802,17020802,18652890 0007,0059,0007 LC CD40 BTK 21441935,21441935,21441935 0663,0006,0007 LC BTK GNB1 7972043 0004 LC BTK GNG2 7972043 0004 LC BTK WAS 22253930,22253930 0007,0096 LC BTK ARID3A 15456761 0004 LC BTK TICAM1 21441935,21441935 0006,0007 LC BTK TIRAP 12724322 0007 LC BTK CMTM3 15087455 0007 LC BTK MLLT1 11007757 0004 LC BTK INPP5D 16601135 0004 LC BTK BLNK 18329366,18329366,15087455 0006,0416,0004 LC BTK TLR2 17020802 0007 LC BTK PIN1 16644721 0007 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q5SUF2 P35991 LUC7L3 BTK 0.183 15102471 Q61074 P35991 PPM1G BTK 0.183 15102471 Q61656 P35991 DDX5 BTK 0.183 15102471 Q61830 P35991 MRC1 BTK 0.183 15102471 P35991 O54941 BTK SMARCE1 0.183 15102471 Q9WUA3 P35991 PFKP BTK 0.183 15102471 Q9Z1T1 P35991 AP3B1 BTK 0.183 15102471 Q62431 P35991 ARID3A BTK 0.21 15456761 P35991 P0C0S6 BTK H2AFZ 0.183 15102471 Q80UY2 P35991 KCMF1 BTK 0.183 15102471 Q8BTX4 P35991 GLTSCR2 BTK 0.183 15102471 P35991 P14148 BTK RPL7 0.183 15102471 P35991 P05064 BTK ALDOA 0.183 15102471 P35991 P29391 BTK FTL1 0.183 15102471 P35991 P28301 BTK LOX 0.183 15102471 Q9ES52 P35991 INPP5D BTK 0.21 16601135 Q60631 P35991 GRB2 BTK 0.183 15102471 P78347 P35991 GTF2I BTK 0.21 9012831 P70315 P35991 WAS BTK 0.183 15102471 P63213 P35991 GNG2 BTK 0.21 7972043 P62874 P35991 GNB1 BTK 0.21 7972043 Q9QUN3 P35991 BLNK BTK 0.454 18329366,15087455 P40694 P35991 IGHMBP2 BTK 0.183 15102471 P62631 P35991 EEF1A2 BTK 0.183 15102471 P48453 P35991 PPP3CB BTK 0.183 15102471 P83917 P35991 CBX1 BTK 0.183 15102471 P68369 P35991 TUBA1A BTK 0.183 15102471 P35991 P15105 BTK GLUL 0.183 15102471 P68033 P35991 ACTC1 BTK 0.183 15102471 P35991 O09159 BTK MAN2B1 0.183 15102471