iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000031930.11	ENSMUST00000166615.2	ENSMUSP00000132224.1
ENSMUSG00000031930.11	ENSMUST00000212205.1	ENSMUSP00000148679.1

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
miRTarBase	microRNA	TRANSFAC	RepTar
RAID2
Protein-protein Interaction
IID	iRefIndex	Mentha
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	mUbiSiDa
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	17496161; 17526739

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	9.70e-135	450.4	537	869

Active Site

Position(s)	Description	Evidence
838	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00104}

Domain Profile

E3 activity/HECT

   S: 2    ksdlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldl 90
           ++dl++rl++ ++gEegld+gG++re+++lls+e+ np+++lf+y  +++++lq+np+s + np+hl +F+f+Gr++++al++g+++d+
   Q: 537  PYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSI-NPDHLTYFRFIGRFIAMALYHGKFIDT 624
           6799********************************************************9.89************************* PP
   S: 91   plsksfyklllgkklsledleevdpelykslkeikenaesdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvve 179
            ++ +fyk++l+k+ +l+dle++dpe+y+s+ +iken   ++ee+gl+l f ++ eilg+++++eLk+gGeni+Vt+eNkeeY+ l+++
   Q: 625  GFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKEN---NLEECGLELFFIQDMEILGKVTTHELKEGGENIRVTEENKEEYIMLLTD 710
           ***********************************99...9************************************************ PP
   S: 180  yrlkegikeqveafreGfneviplellslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFv 268
           +r+++g++eq++af +Gfnev ple+l++F+e+elel++cG++eid++d++k++ y++ yt++s++iqwFw+v++e+++e+r ++LqFv
   Q: 711  WRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKNAIYRH-YTKSSKQIQWFWQVVKEMDNEKRIRLLQFV 798
           ********************************************************99.****************************** PP
   S: 269  TgssrlPlgGfaelk....prlfvirkdasddrlPssnTCfnyLkLPeYsskeilkekLlyAieetkgFel 335
           Tg++rlP+gGfael     p++f+i + +++++lP+s+TCfn+L+LP+Y+s+e+lkekLlyAieet+gF++
   Q: 799  TGTCRLPVGGFAELIgsngPQKFCIDRVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLYAIEETEGFGQ 869
           *********************************************************************96 PP

Organism

Mus musculus

Functional Description
(View)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; regulates POU5F1 protein level during differentiation of embryonal carcinoma cells (ECCs) but not in undifferentiated ECCs and embryonic stem cells (ESCs). Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.

Protein Sequence
(Fasta)

MASASSSRAG VALPFEKSQL TLKVVSAKPK VHNRQPRINS YVEVAVDGLP SETKKTGKRI 60
GSSELLWNEI IVLNVTAQSH LDLKVWSCHT LRNELLGTAS VNLSNVLKNN GGKMENTQLT 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ACGTGACCCG GCCCGAGTGC GGGCGCTGGA AGGCGGAAGT GGAGCGGAGT TAGGCGCCGC 60
TGGTGTGGCC GCGGCAGGTA GGTGGCGCGG GGGCACTGGG GGGCTCGGCA GCGAGCGGGC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR000008--C2_dom
IPR024928--E3_ub_ligase_SMURF1
IPR000569--HECT_dom
IPR001202--WW_dom

PROSITE

PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2

Pfam

PF00632--HECT
PF00397--WW

SMART

SM00239--C2
SM00119--HECTc
SM00456--WW

Gene Ontology

GO:0005737--C:cytoplasm
GO:0070062--C:extracellular exosome
GO:0016020--C:membrane
GO:0005634--C:nucleus
GO:0001085--F:RNA polymerase II transcription factor binding
GO:0008134--F:transcription factor binding
GO:0001190--F:transcriptional activator activity, RNA polymerase II transcription factor binding
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0010629--P:negative regulation of gene expression
GO:0051224--P:negative regulation of protein transport
GO:0043433--P:negative regulation of sequence-specific DNA binding transcription factor activity
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0032410--P:negative regulation of transporter activity
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0051865--P:protein autoubiquitination
GO:0070534--P:protein K63-linked ubiquitination
GO:0016567--P:protein ubiquitination
GO:0034765--P:regulation of ion transmembrane transport
GO:0042391--P:regulation of membrane potential
GO:1901016--P:regulation of potassium ion transmembrane transporter activity

KEGG

mmu:66894

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001575	Ailuropoda melanoleuca	94.84	0.00e+00	1641.00
IUUC-Apl-003912	Anas platyrhynchos	76.20	0.00e+00	1240.00
IUUC-Aca-004291	Anolis carolinensis	80.90	0.00e+00	1434.00
IUUC-Bta-013231	Bos taurus	94.25	0.00e+00	1599.00
IUUC-Cja-019455	Callithrix jacchus	95.63	0.00e+00	1683.00
IUUC-Cfa-021017	Canis familiaris	95.29	0.00e+00	1630.00
IUUC-Cpo-021988	Cavia porcellus	95.29	0.00e+00	1664.00
IUUC-Csa-023549	Chlorocebus sabaeus	95.52	0.00e+00	1691.00
IUUC-Dre-028513	Danio rerio	91.59	0.00e+00	1138.00
IUUC-Dno-028976	Dasypus novemcinctus	94.72	0.00e+00	1655.00
IUUC-Dor-030176	Dipodomys ordii	93.03	0.00e+00	1513.00
IUUC-Eca-033949	Equus caballus	95.17	0.00e+00	1644.00
IUUC-Eeu-034529	Erinaceus europaeus	86.81	0.00e+00	1360.00
IUUC-Fca-036236	Felis catus	95.06	0.00e+00	1667.00
IUUC-Fal-036948	Ficedula albicollis	78.21	0.00e+00	1332.00
IUUC-Gmo-038842	Gadus morhua	88.89	0.00e+00	1067.00
IUUC-Gga-040617	Gallus gallus	79.89	0.00e+00	1437.00
IUUC-Gac-041338	Gasterosteus aculeatus	69.44	0.00e+00	1253.00
IUUC-Ggo-045278	Gorilla gorilla	95.98	0.00e+00	1696.00
IUUC-Hsa-046148	Homo sapiens	95.98	0.00e+00	1697.00
IUUC-Itr-047945	Ictidomys tridecemlineatus	97.94	0.00e+00	1122.00
IUUC-Lch-050663	Latimeria chalumnae	78.03	0.00e+00	1390.00
IUUC-Loc-052604	Lepisosteus oculatus	76.22	0.00e+00	1321.00
IUUC-Laf-053944	Loxodonta africana	94.83	0.00e+00	1664.00
IUUC-Mcc-055655	Macaca mulatta	95.63	0.00e+00	1692.00
IUUC-Meu-055953	Macropus eugenii	79.47	0.00e+00	1385.00
IUUC-Mga-060190	Meleagris gallopavo	80.50	0.00e+00	1434.00
IUUC-Mmr-060896	Microcebus murinus	96.42	0.00e+00	1649.00
IUUC-Mdo-062536	Monodelphis domestica	90.60	0.00e+00	1613.00
IUUC-Mpu-066608	Mustela putorius furo	92.89	0.00e+00	1584.00
IUUC-Mlu-067623	Myotis lucifugus	92.08	0.00e+00	1591.00
IUUC-Nle-069496	Nomascus leucogenys	94.03	0.00e+00	1649.00
IUUC-Opr-071054	Ochotona princeps	83.51	0.00e+00	1423.00
IUUC-Ont-071279	Oreochromis niloticus	74.36	0.00e+00	1282.00
IUUC-Oan-073512	Ornithorhynchus anatinus	86.33	0.00e+00	1492.00
IUUC-Ocu-074271	Oryctolagus cuniculus	94.98	0.00e+00	1624.00
IUUC-Ola-087237	Oryzias latipes	69.43	0.00e+00	1228.00
IUUC-Oga-088164	Otolemur garnettii	96.09	0.00e+00	1678.00
IUUC-Oar-089698	Ovis aries	94.60	0.00e+00	1613.00
IUUC-Ptr-091306	Pan troglodytes	94.71	0.00e+00	1667.00
IUUC-Pan-092814	Papio anubis	92.09	0.00e+00	1601.00
IUUC-Pfo-096709	Poecilia formosa	72.89	0.00e+00	1283.00
IUUC-Pab-098494	Pongo abelii	90.39	0.00e+00	1602.00
IUUC-Pca-100596	Procavia capensis	83.10	0.00e+00	1379.00
IUUC-Pva-103154	Pteropus vampyrus	90.37	0.00e+00	1559.00
IUUC-Rno-104954	Rattus norvegicus	97.49	0.00e+00	1440.00
IUUC-Sha-106781	Sarcophilus harrisii	90.71	0.00e+00	1644.00
IUUC-Ssc-116307	Sus scrofa	83.63	0.00e+00	1436.00
IUUC-Tgu-116530	Taeniopygia guttata	75.74	0.00e+00	1185.00
IUUC-Tru-118287	Takifugu rubripes	89.63	0.00e+00	1068.00
IUUC-Tsy-119097	Tarsius syrichta	82.97	0.00e+00	1231.00
IUUC-Tni-120801	Tetraodon nigroviridis	68.29	0.00e+00	1151.00
IUUC-Tbe-127793	Tupaia belangeri	83.91	0.00e+00	1435.00
IUUC-Ttr-128275	Tursiops truncatus	90.34	0.00e+00	1548.00
IUUC-Xtr-132866	Xenopus tropicalis	82.70	0.00e+00	1134.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence